• Title/Summary/Keyword: genetic variability

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Characterization of Indian Riverine Buffaloes by Microsatellite Markers

  • Sukla, Soumi;Yadav, B.R.;Bhattacharya, T.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.11
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    • pp.1556-1560
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    • 2006
  • Six breeds of riverine buffalo viz. Murrah, Mehsana, Jaffrabadi, Nagpuri, Nili-Ravi and Bhadawari were characterized using FAO-recommended cattle specific microsatellite markers. Among the total of twenty microsatellite markers screened to explore genomic variability of six buffalo breeds, only ten were polymorphic in nature. Four out of ten polymorphic microsatellite loci were rated as informative. The numbers of alleles detected ranged from 2 to 7, with a mean of $5.5{\pm}0.07$ per microsatellite marker. The most polymorphic marker was BM1818 with a total of 7 alleles present at this locus. One breed specific marker was found in each of Mehsana (BM1818) and Bhadawari (ILSTS030) and four were found in Jaffarabadi (BM1818, ILSTS030, ILSTS054 and ILSTS011). Genetic distance (Ds) between the Mehsana and Bhadawari breed was the maximum (0.29), followed by Murrah and Mehsana (0.27), and Nili-Ravi and Bhadawari (0.26). The lowest Ds was found between the Jaffrabadi and Nagpuri breeds which was only 0.05. The highest divergence time of 1318 years was established between Mehsana and Bhadawari breeds whereas it was found to be lowest (272 years) between the Jaffrabadi and Nagpuri breeds.

Molecular Phylogenetics of Centrocestus formosanus (Digenea: Heterophyidae) Originated from Freshwater Fish from Chiang Mai Province, Thailand

  • Wongsawad, Chalobol;Wongsawad, Pheravut;Sukontason, Kom;Maneepitaksanti, Worawit;Nantarat, Nattawadee
    • Parasites, Hosts and Diseases
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    • v.55 no.1
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    • pp.31-37
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    • 2017
  • This study aimed to investigate the morphology and reconstruct the phylogenetic relationships of Centrocestus formosanus originating from 5 species of freshwater fish, i.e., Esomus metallicus, Puntius brevis, Anabas testudineus, Parambassis siamensis, and Carassius auratus, in Chiang Mai province, Thailand. Sequence-related amplified polymorphism (SRAP) and phylogeny based on internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome c oxidase subunit 1 (CO1) were performed. The results showed similar morphologies of adult C. formosanus from day 5 after infection in chicks. C. formosanus originated from 4 species of freshwater fish had the same number of circumoral spines on the oral sucker, except for those from C. auratus which revealed 34 circumoral spines. The phylogenetic tree obtained from SRAP profile and the combination of ITS2 and CO1 sequence showed similar results that were correlated with the number of circumoral spines in adult worms. Genetic variability of C. formosanus also occurred in different species of freshwater fish hosts. However, more details of adult worm morphologies and more sensitive genetic markers are needed to confirm the species validity of C. formosanus with 34 circumoral spines originating from C. auratus in the future.

Study on Inheritance and Transgressions at Cocoon and Shell Weight Characters between Genetically Sex-limited Silkworm Bombyx mori L. Lines

  • Petkov N.;Petkov Z.;Grekov D.;Arnaudova K.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.11 no.1
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    • pp.75-78
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    • 2005
  • Inheritance of cocoon and shell weight characters in silkworm crosses between sex-limited at larva stage lines, including three (NIG$_{1}$ $\times$ TV$_{3/2}$ ,NIG$_{1}$ $\times$ TBV$_{2/24}$ and NIG$_{1}$ $\times$ B$_{2/6}$ ) from Japanese type and three (NIG$_{2}$ $\times$ B$_{2/6}$ ,TV$_{3/2}$ $\times$ NIG$_{1}$ and NIG$_{2}$ $\times$ TBV$_{2/24}$ ) from Chinese type was studied at Regional Centre for Scientific­Applied Service - Vratza during the period of 2000 ­2002. Inheritance of tested characters in F I was deter­mined through genetic parameters, additiveness (a), dominance (d) and their ratio (d/a). Degree and frequency of transgressions in F$_{2}$ were calculated also. Over dominance (d/a(>) 1) with bigger contribution of the parent with higher value was determined at inheritance tested characters. Transgressions obtained at cocoon and shell weight characters were positive and varied in wide limits. The productivity of parent's lines had defined the variability of genetic parameters for degree and frequency of transgressions. Combination of positive transgressions at cocoon and shell weight characters in F2 hybrid populations from sex-limited at larva stage allow us to select individuals with high productive potential for further use in selection programs.

The Distribution of Genetic Polymorphism in the ACE2 Gene in Korean Essential Hypertensives (한국인 본태성 고혈압 환자군에서 ACE2유전자에 존재하는 A1075G다형성의 분포에 관한 연구)

  • Jang Min Hee;Kang Byung Yong;Lee Jae Koo;Lee Kang Oh
    • Environmental Analysis Health and Toxicology
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    • v.20 no.4 s.51
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    • pp.303-309
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    • 2005
  • Essential hypertension has been considered as multifactorial disease resulted from the interaction of both environmental and genetic factors. The renin-angiotensin system (RAS) plays an important role in the regulation of blood pressure homeostasis. Recently, a homologue of angiotensin I converting enzyme, ACE2 has been focused on as a candidate gene of essential hypertension in the experiments using animal model and human being. In this study, we carried out an association study in order to clarify the relationship between the A 1075G polymorphism in the ACE2 gene and essential hypertension in Korean subjects. Because this polymorphism is located on human chromosome X, the statistical analysis for each gender was performed separately. There were no significant differences in allele distribution of the A 1075G polymorphism in the ACE2 gene between normotensives and hypertensives in the both gender groups, respectively. However, this polymorphism was significantly associated with systolic blood pressure (SBP) and diastolic blood pressure (DBP) values in only female groups (P< 0.05). Thus, these results may suggest the probable role of ACE2 gene in the inter-individual susceptibility of female group to blood pressure variability.

Mitochondrial DNA Diversity and Origin of Red Chittagong Cattle

  • Bhuiyan, M.S.A.;Bhuiyan, A.K.F.H.;Yoon, D.H.;Jeon, J.T.;Park, C.S.;Lee, J.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.10
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    • pp.1478-1484
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    • 2007
  • To determine the origin and genetic diversity of Red Chittagong (RC) cattle in Bangladesh, we analyzed mitochondrial DNA displacement loop (D-loop) sequences of 48 samples along with 22 previously published sequences from Bos indicus and Bos taurus breeds. Twenty five haplotypes were identified in RC cattle that were defined by 44 polymorphic sites and nucleotide diversity was $0.0055{\pm}0.0026$. The estimated sequence divergence times between RC and other zebu cattle breeds studied ranged between 22,700-26,900 years before present (YBP) which, it is suggested, predate domestication of RC cattle. Furthermore, it is assumed that introgressions have occurred in this breed mainly from Indian zebu breeds in the recent millennia. The phylogenetic studies showed RC cattle clustered with Bos indicus lineage with two distinct haplogroups representing high genetic variability of this breed. These findings can be used for designing proper breeding and conservation strategies for RC cattle in Bangladesh.

Growth and RAPD Variation of Enteromorpha prolifera (Oeder) J. Agardh, (Ulvaceae, Chlorophyta) from Korea

  • Yoon Jang-Taek;Chung Gyu-Hwa
    • Fisheries and Aquatic Sciences
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    • v.5 no.3
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    • pp.156-164
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    • 2002
  • Enteromorpha prolifera of the isomorphic diploid sporophyte and the haploid gametophyte generations inhabit rocks, tidal flats and tidal pools in the middle parts of intertidal zones. In this experiment, their thalli were observed by bare eyes from October and experienced $74\pm16.5cm$ maximum growth the following March and April. The rate of occurrence of the thalli per month was highest in March, while their biomass peaked at $1,464\pm41.5 g/m^2$ in Jangheung in April. Genetic similarity was investigated samples of E. prolifera collected from Muan, Wando, Jangheung, Yosu and Jinhae, at the south coast of Korea. Random amplified polymorphic DNA (RAPD) markers were used. For the RAPD analysis, 3 ng of the DNA extracted from the thalli using he phenol/chloroform method was amplified by PCR with a 25 {\mu}L$ reaction solution, arbitrary primers and 36 cycles. Among the 60 primers used, 31 yielded products, most of which showed diverse electrophoresis patterns. Similarities among the groups compared ranged from 0.37 to 0.58. We conclude that the use of RAPD analysis is appropriate to characterize the genetic variability of this commercial species along its geographical distribution.

Epidemiological Concepts and Strategies in Breeding Soybeans for Disease Resistance

  • Seung Man, Lim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.35 no.1
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    • pp.97-107
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    • 1990
  • The epidemiology of plant disease deals with the dynamic processes of host-pathogen interactions, which determine the prevalence and severity of the disease. Epidemic processes for most foliar diseases of plants follow a series of steps: arrival of pathogens on plant surfaces, initial infection, incubation period, latent period, sporulation, dissemination of secondary inoculum, and infectious period. These complex biological processes are influenced by the environment-Man also often interfers with these processes by altering the host and pathogen populations and the environment. Slowing or halting any of the epidemic processes can delay the development of the epidemic, so that serious losses in yield due to disease do not occur. It is generally recognized that the most effective and efficient method of minimizing disease damage is through the use of resistant cultivars, particularly when other methods such as fungicide applications are not economically feasible-Populations of plant pathogens are not genetically uniform nor are they necessarily stable. Cultivars bred for resistance to current populations of a pathogen may not be resistant in the future due to selection pressures placed on the pathogen populations. Understanding population development and genetic variability in the pathogen, and knowledge of the genetics of resistance in the plant should help in developing breeding strategies that wi1l provide effective and stable disease control through genetic resistance. In the United States, soybeans have ranked first in value of crops sold off the farm in recent years. Soybeans have been the leading U. S.

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Current Status of Wheat Transformation

  • Rakszegi, Marrian;Tamas, Cecilia;Szucs, Peter;Tamas, Laszlo;Bedo, Zoltan
    • Journal of Plant Biotechnology
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    • v.3 no.2
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    • pp.67-81
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    • 2001
  • Traditionally, genetic variability is generated by an extensive crossing program, which is complemented by strict selection to identify useful new recombinants. Plant biotechnology offers many opportunities for breeders to solve certain breeding problems at the molecular level. The tissue culture methodology and the genetic modification of economically important monocotyledons have undergone a revolution in the last decade. As the production of transgenic plants is a complex procedure, including the uptake of DNA molecules into the cells, the integration of foreign nucleotide sequences into the host genomic DNA and the expression of new genes in a controlled way, and as there are still many unsolved questions, further development is necessary. The methodology opens up the possibility of introducing novel genes that may induce resistance to diseases and abiotic stresses, allow the modification of dough quality and the dietetic quality of proteins, and increase the levels of micronutrients such as iron, zinc, and vitamins. In the present review, the authors would like to summarise the most important advances in wheat transformation.

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Gene Constitution of Egg White Proteins of Native Chicken in Asian Countries

  • Kinoshita, K.;Okamoto, S.;Shimogiri, T.;Kawabe, K.;Nishida, T.;Kakizawa, R.;Yamamoto, Y.;Maeda, Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.2
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    • pp.157-165
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    • 2002
  • Genetic variations of seven egg white protein loci in 1,112 samples from eight Asian countries (Yunnan province of China, Mongolia, Nepal, Vietnam, Laos, Thailand, Myanmar, Indonesia) and 360 samples from two improved breeds (Isa Brown, Boris Brown) were investigated by using starch gel and polyacrylamide gel electrophoresis. Five egg white protein loci (Ov, $G_3$, $G_2$, $G_1$ and $Tf_{EW}$) were found to be polymorphic in Asian native chicken populations. The proportion of polymorphic loci ($P_{poly}$) and average heterozygosity ($\bar{H}$) of Asian native populations varied from 0.143 to 0.714 and 0.014 to 0.225, respectively, and were higher than those of improved breeds. The subdivision index ($G_{ST}$) value among 18 native chicken populations in Asia is lower (0.0827) than among improved chicken populations (0.1693). This value means that the degree of subdivision among Asian native populations is lower than among improved breeds and gene constitutions among populations in Asia are similar.

Adaptive Application Component Mapping for Parallel Computation Offloading in Variable Environments

  • Fan, Wenhao;Liu, Yuan'an;Tang, Bihua
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.9 no.11
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    • pp.4347-4366
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    • 2015
  • Distinguished with traditional strategies which offload an application's computation to a single server, parallel computation offloading can promote the performance by simultaneously delivering the computation to multiple computing resources around the mobile terminal. However, due to the variability of communication and computation environments, static application component multi-partitioning algorithms are difficult to maintain the optimality of their solutions in time-varying scenarios, whereas, over-frequent algorithm executions triggered by changes of environments may bring excessive algorithm costs. To this end, an adaptive application component mapping algorithm for parallel computation offloading in variable environments is proposed in this paper, which aims at minimizing computation costs and inter-resource communication costs. It can provide the terminal a suitable solution for the current environment with a low incremental algorithm cost. We represent the application component multi-partitioning problem as a graph mapping model, then convert it into a pathfinding problem. A genetic algorithm enhanced by an elite-based immigrants mechanism is designed to obtain the solution adaptively, which can dynamically adjust the precision of the solution and boost the searching speed as transmission and processing speeds change. Simulation results demonstrate that our algorithm can promote the performance efficiently, and it is superior to the traditional approaches under variable environments to a large extent.