• Reproductive and Developmental Biology
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• 제29권4호
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• pp.207-212
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• 2005

In the present study, we investigated the effects of genotypes on in vitro maturation and fertilization in porcine fresh/frozen-thawed oocytes. The porcine cumulus-oocyte complexes (COCs) were divided into four groups according to whether they were: (1) in vitro matured; (2) cryopreserved and in vitro matured; (3) in vitro fertilized and (4) cryopreserved, and in vitro fertilized. Maturation of porcine COCs was accomplished by incubation in NCSU23 medium. Immature oocytes were cryopreserved by Open Pulled Straws (OPS) method according to Vajta et al., (1998). Oocytes stained by Acetic-Orcein method were observed under the microscope. DNA extracted from the ovaries was analyzed by RAPD (random amplified polymorphic DNA) and SSCP (single strand conformational polymorphisrrt) method. The rates of oocytes maturation and fertilization were significantly high in AA genotype. The results indicated that in vitro maturation and fertilization in porcine fresh/frozen-thawed oocytes may be affected by genotypes in pigs.

• 한국경영과학회:학술대회논문집
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• 대한산업공학회/한국경영과학회 2004년도 춘계공동학술대회 논문집
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• pp.167-170
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• 2004

This paper proposes resource allocation method for achieving investment goals in manufacturing system. In order to align resource allocation and manufacturing system design, the system design decomposition (SDD) approach is used. In this paper, a mathematical formulation for resource allocation based on SDD approach is analyzed and a genetic algorithm application is discussed.

• Journal of Microbiology and Biotechnology
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• 제14권6호
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• pp.1318-1323
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• 2004

This study describes the purification and characterization of a novel antihypertensive angiotensin 1­converting enzyme (ACE) inhibitory peptide from Saccharomyces cerevisiae. Maximal production of the ACE inhibitor from Saccharomyces cerevisiae was obtained from 24 h of cultivation at $30^{\circ}C$ and its ACE inhibitory activity was increased by about 1.5 times after treatment of the cell-free extract with pepsin. After the purification of ACE inhibitory peptides with ultrafiltration, Sephadex G-25 column chromatography, and reverse-phase HPLC, an active fraction with an $IC_{50}$ of 0.07 mg and $3.5\%$ yield was obtained. The purified peptide was a novel decapeptide, showing very low similarity to other ACE inhibitory peptide sequences, and its amino acid sequence was Tyr-Asp-Gly-Gly-Val-Phe-Arg-Val-Tyr-Thr. The purified inhibitor competitively inhibited ACE and also showed a clear antihypertensive effect in spontaneously hypertensive rats (SHR) at a dosage of 1 mg/kg body weight.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제11권4호
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• pp.1972-1986
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• 2017

With the advent of the knowledge economy, science and technology resources have played an important role in economic competition, and their optimal allocation has been regarded as very important across the world. Thus, allocation optimization research for regional science and technology resources is significant for accelerating the reform of regional science and technology systems. Regional science and technology resource allocation optimization is modeled as a double-layer optimization model: the entire system is characterized by top-layer optimization, whereas the subsystems are characterized by bottom-layer optimization. To efficaciously solve this optimization problem, we propose a mixed search method based on the orthogonal genetic algorithm and sensitivity analysis. This novel method adopts the integrated modeling concept with a combination of the knowledge model and heuristic search model, on the basis of the heuristic search model, and simultaneously highlights the effect of the knowledge model. To compare the performance of different methods, five methods and two channels were used to address an application example. Both the optimized results and simulation time of the proposed method outperformed those of the other methods. The application of the proposed method to solve the problem of entire system optimization is feasible, correct, and effective.

• Asian-Australasian Journal of Animal Sciences
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• 제23권7호
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• pp.848-854
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• 2010

The objectives of this study were to estimate genetic parameters for the carcass price and carcass traits contributing to carcass grading and to investigate the influence of each carcass trait on the carcass price using multiple regression and path analyses. Data for carcass traits and carcass prices were collected from March 2003 to January 2009 on steers of Korean cattle raised at private farms. The analytical mixed animal model, including slaughter house-year-month combination, linear and quadratic slaughter age as fixed effects and random animal and residual effects, was used to estimate genetic parameters. The effects of carcass traits on the carcass price were evaluated by applying multiple regression analyses. Heritability estimates of carcass traits were $0.20{\pm}0.08$ for carcass weight (CWT), $0.33{\pm}0.10$ for back fat thickness (BFT), $0.07{\pm}0.05$ for eye-muscle area (EMA) and $0.25{\pm}0.10$ for marbling score (MS), and those of carcass prices were $0.21{\pm}0.10$ for auction price per 1 kg of carcass weight (AP) and $0.13{\pm}0.07$ for total price (CP). Genetic correlation coefficients of AP with CWT and MS were $-0.35{\pm}0.29$ and $0.99{\pm}0.04$, respectively, and those of CP with CWT and MS were $0.59{\pm}0.22$ and $0.39{\pm}0.29$ respectively. If an appropriate adjustment for temporal economic value is available, the moderate heritability estimates of AP and CP might suggest their potential use as the breeding objectives for improving the gross incomes of beef cattle farms. The large genetic correlation estimates of carcass price variables with CWT and MS implied that simultaneous selection for both CWT and MS would be also useful in enhancing income.

• 한국초지조사료학회지
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• 제18권4호
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• pp.277-284
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• 1998

재배콩과 돌콩의 유전적 다양성과 유연관계를 밝히고, 콩을 사료작물로 개량하는데 필요한 유전정보를 얻기 위하여, RAPD 분석방법을 이용한 결과는 다음과 같다. 1. 증폭된 polymorphicband의 총 수는 74개였으며, 이중 다형현상을 보인 것은 50개로 67.6%, 증폭된 band의 크기는 0.13~2.0 Kb 범위에 있었다. 2. Random primer의 sequence 5'-CAG GCC CTT C-3'를 사용하였을 때, 0.56-2.0 Kb에서 콩의 종피 색깔에 따른 변이 (누런색과 검은색)가 나타났다. 그리고 Sequence 5'-TGC TCT GCC C-3'과 5'-GTC CAC ACG G-3'인 primer를 사용하였을 때, 각각 0.63~0.94, 0.94~2.0 Kb에서 검정콩 2호에 특정적인 genetic marker가 검출되었다. 3. Genetic similarity 값에 의한 품종간 유전적 변이성은 검정콩 2호 (0.81)가 가장 낮았고 검정콩 1호 (0.46)에서 가장 높았다. 4. 콩의 품종과 돌콩의 유연관계는 황금콩과 석량붓콩이 0.52로 가장 가까웠고 검정올콩 (0.47), 검정콩 l호 (0.46), 검정콩 2호 (0.42), 돌콩 (0.38)의 순서로 멀었다.

• The Plant Pathology Journal
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• 제40권4호
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• pp.390-398
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• 2024

The Chinese artichoke (Stachys affinis syn. S. sieboldii) is a widely cultivated crop, and its rhizome is used as a medicinal vegetable. To investigate the causes of viral diseases in Chinese artichokes, the infection rates of four virus species infecting Chinese artichoke were investigated. Since the Chinese artichoke propagates through its tuber, this study aimed to determine whether viral transmission to the progeny is possible through the tuber, by identifying the virus present in the tuber and investigating its accumulation. First, reverse transcription polymerase chain reaction analysis was performed to detect viruses using total RNA extracted from the flowers, leaves, and tubers of Chinese artichoke plants. Alfalfa mosaic virus (AMV) and Chinese artichoke mosaic virus (ChAMV) had high infectivity in Chinese artichoke and most plants were simultaneously infected with AMV and ChAMV. These viruses were present in all tissues, but their detection frequency and accumulation rates varied across different tissues of the Chinese artichoke. Also, we sequenced the coat protein (CP) genes of AMV and ChAMV to investigate genetic variations of virus between the leaf and tuber. It provides information on CP gene sequences and genetic diversity of isolates identified from new hosts of AMV and ChAMV. This study offers valuable insights into the distribution and spread of the ChAMV and AMV within Chinese artichoke plants, which have implications for the management and control of viral infections in crops.

• Asian-Australasian Journal of Animal Sciences
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• 제16권7호
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• pp.939-945
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• 2003

Seventeen Chinese indigenous pig breeds and three introduced pig breeds had been carried out by means of vertical polyacrylamide gel electrophoresis (PAGE). According to the results, eight serum protein loci were highly polymorphic except Pi-2 and Cp. The polymorphism information content (PIC) of Hpx was the highest (0.5268), while that of Cp was the lowest (0.0257). The population genetic variation index showed that about 84% genetic variation existed in the population, and the rest of 16% distributed between the populations. The genetic variation of Yimeng black pig and Duroc were the highest and the lowest, respectively. The genetic variation of Chinese indigenous pig breeds was much more than that of exotic groups. Genetic distance results showed that Chinese indigenous pig breeds were classified into four groups with the three introduced pig breeds clustered into another group. The results also supported the geographic distribution of Chinese indigenous pig breeds in certain extent.

• 한국건설관리학회논문집
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• 제8권6호
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• pp.235-245
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• 2007

This research study presents the development and application of an Elitist Genetic Algorithm (Elitist GA) for solving the resource-constrained project scheduling problem, which is one of the most challenging problems in construction engineering. Main features of the developed algorithm are that the elitist roulette selection operator is developed to preserve the best individual solution for the next generation so as to obtain the improved solution, and that parallel schedule generation scheme is used to generate a feasible solution to the problem. The experimental results on standard problem sets indicate that the proposed algorithm not only produces reasonably good solutions to the problems over the heuristic method and other GA, but also can find the optimal and/or near optimal solutions for the large-sized problems with multiple resources within a reasonable amount of time that will be applicable to the construction industry. This paper will help researchers and/or practitioners in the construction project scheduling software area with alternative means to find the optimal schedules by utilizing the advantages of the Elitist GA.

• International Journal of Internet, Broadcasting and Communication
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• 제16권3호
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• pp.16-26
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• 2024

Mobile edge computing (MEC) plays a crucial role in improving the performance of resource-constrained mobile devices by offloading computation-intensive tasks to nearby edge servers. However, existing methods often neglect the critical consideration of future task requirements when making offloading decisions. In this paper, we propose an innovative approach that addresses this limitation. Our method leverages recurrent neural networks (RNNs) to predict task sizes for future time slots. Incorporating this predictive capability enables more informed offloading decisions that account for upcoming computational demands. We employ genetic algorithms (GAs) to fine-tune fitness functions for current and future time slots to optimize offloading decisions. Our objective is twofold: minimizing total processing time and reducing energy consumption. By considering future task requirements, our approach achieves more efficient resource utilization. We validate our method using a real-world dataset from Google-cluster. Experimental results demonstrate that our proposed approach outperforms baseline methods, highlighting its effectiveness in MEC systems.