• 제목/요약/키워드: genetic relatedness

검색결과 82건 처리시간 0.027초

Genetic Relatedness within Streptococcus pneumoniae Serotype 19F and 23F Isolates in Korea by Pulsed-Field Gel Electrophoresis

  • Lee, Kwang-Jun;Bae, Song-Mee;Hwang, Kyu-Jam;Lee, Young-Hee;Kim, Ki-Sang
    • Journal of Microbiology
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    • 제41권1호
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    • pp.1-6
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    • 2003
  • The genetic relatedness of multidrug-resistant pneumococcal isolates of serotypes 19F and 23F was investigated. The DNA fragments digested with Sma I were resolved by pulsed-field gel electrophoresis (PFGE). PFGE analysis of 365. pneumoniae isolates showed 13 different patterns. Among 22 isolates of serotype 19F, 9 different PFGE patterns were present and 14 isolates of serotype 23F isolates represented 5 distinct PFGE patterns. Two isolates of serotype 19F and six isolates of serotype 23F shared the same PFGE pattern (Pattern I). Based on the genetic relatedness within the strains (one genetic cluster was defined as having more than 85% homology), we divided the pneumococcal strains into genefic clusters (Ⅰ, II, III, IV, V, and VI). The 22 strains of serotype 19F belonged to five distinct genetic clusters (I, II, III, IV, V and VI) and 14 strains of serotype 23F represented two genetic clusters (I and II ). These results showed that strains of serotype 19F are genetically more diverse than those of serotype 23F, Serotype 19F isolates with PFGE patterns H and I appeared to be less related to those of the remaining PFCE patterns (A to G) (less than 60% genetic relatedness), but those strains were genetically closely related with serotype 23f. These results suggest that the latter isolates originated from horizontal transfer of the capsular type 19F gene locus to 23F pneumococcal genotypes. In conclusion, the multidrug-resistant pneumococcal isolates of serotype 19f and 23F isolated in Korea are the result of the spread of a limited number of resistant clones.

Fuarium oxysporum의 유연관계 분석을 위한 Recombinant DNA의 Probe로서의 이용 가능성 (Potential Applications of Recombinant DNA Probes for Relatedness Analysis of Fusarium oxysporum)

  • 김홍기;김영태;유승헌
    • 한국식물병리학회지
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    • 제10권1호
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    • pp.1-6
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    • 1994
  • Randomly chosen recombinant clones of Fusarium oxysporum were analysed to select useful probes for relatedness analysis of Fusarium oxysporum. Genomic DNA of F. oxysproum f. sp. cubense, digested with HindIII, was ligated to pUC118 and used to transform Escherichia coli strai DH5$\alpha$. Three clones were identified that hybridized to mutiple restriction fragments of some formae speciales of F. oxysporum. These probes detected repetitive sequences in HindIII or EcoRI digested DNAs. Repeated copy clone pFC46, pFC52 and pFC54 showed evident polymorphisms among ten formae speciales of this fungus. Since clone pFC 52 strongly hybridized to multiple EcoRI-digested restriction fragments of f. sp. cubense, it may be useful as a probe for analysis of other genetic characteristics of this forma specialis. The results suggest that our clones might be very useful as probes for relatedness analysis between or within formae speciales of Fusarium oxysporum.

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한국산 까마귀과(참새목) 조류의 계통 진화 (Systematics and Evolutionary Study of the Family Corvidae(Passeriformes) in Korea)

  • 심재한;박병상;양서영
    • 한국환경생태학회지
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    • 제11권3호
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    • pp.277-289
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    • 1997
  • 참새목(Passeriformes) 조류인 까마귀과 조류 4속 4종과 물까마귀과의 물까마귀를 대상으로 전기영동실험방법을 이용하여 유전적 근연관계와 종간의 분화ㅏ연대를 분석한 결과, 까마귀과 4속과 두 개의 과는 다른 조류 분류군과 유사한 정도의 유전적 근연치를 나타내었으나, 조류를 제외한 다른 분류군보다는 근연치가 낮았다. 유전적 근연관계를 분석한 결과 어치(Garrulus glandarius brandtii)와 까마귀(Corvus corone orientalis)는 S=0.81로 가장 가까웠으며, 다음으로 까치(Pica pica sericea)가 S=0.72로 나타났다. 한편 물까치(Cyanopica cyanus koreensis)는 S=0.68로 까마귀과 조류 중 가장 근연치가 멀게 나타났다. 유전적 차이치로 5종간의 종 분화연대를 추산한 결과 물까마귀과 조류는 까마귀와 약 390만년 전에 분화된 것으로 나타났고 까마귀과 조류 4종의 겨웅 물까치는 약 190만년 전에, 까치는 약 160만년 전에 각각 분화되었고, 약 100만년 전에 어치와 까마귀가 분화된 것으로 추정된다.

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Molecular Analysis of Exophiala Species Using Molecular Markers

  • Chee, Hee-Youn;Kim, Yoon-Kyoung
    • Mycobiology
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    • 제30권1호
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    • pp.1-4
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    • 2002
  • Genetic relatedness of medically important Exophiala species such as E. dermatitidis, E. mansonii, and three E. jeanselmei varieties: jeanselmei, lecanii-corni, and heteromorpha was examined using PCR-RFLP(restriction fragment length polymorphism) of ribosomal DNA, M-13, $(GTG)_5$ and nucleotide sequences of ribosomal ITS(internal transcribed space) II regions. Three E. jeanselmei varieties showing distinct band patterns for each DNA markers as well as different nucleotide sequences of ribosomal ITS II regions could be considered as a separate species. E. dermatitidis and E. mansonii demonstrated the identical band patterns of RFLP of ribosomal DNA, M-13, and $(GTG)_5$ markers. However, nucleotides sequences of ribosomal ITS II region were different between these two species.

Multi Trait Selection with Restriction for Cutup Carcass Value in Broiler Chicken: Genetic Relatedness of Lines Involved Based on Randomly Amplified Polymorphic DNA

  • Khosravinia, Heshmatollah;Murthy, H.N.N.;Ramesha, K.P.;Govindaiah, M.G.
    • Asian-Australasian Journal of Animal Sciences
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    • 제18권11호
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    • pp.1535-1541
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    • 2005
  • Five broiler chicken lines, namely HC, BPB2, CPB2, PB2 and UM1, involving in a selection program and differing in selection intensity and genetic background, were screened for randomly amplified polymorphic DNA (RAPD) polymorphism using 10 selected decamer primers. Nine primers amplified the genomic DNA, generating 200 to 2,500 bp and all detected polymorphism between lines. Out of 74 bands scored using these primers, 34 (50.0%) were found to be polymorphic. The number of polymorphic loci ranged from 3 to 6 with an average of 4.33. Lines differed considerably for within-population genetic similarity estimated by band frequency (WS = 93.55 to 99.25). Between-line genetic similarity estimates based on band sharing as well as on band frequency ranged from 71.35 to 86.45 and from 73.38 to 87.68, respectively. Lines HC and PB2 were the most closely related to the other, while BPB2 and CPB2 appeared to be more distant from each other. The between-line genetic distance based on both band sharing and band frequency revealed the similar trends as for Between-line genetic similarity. Based on BS and BF criteria, BPB2 and CPB2 as well as PB2 and UM1 lines can be merged to launch a new genetic group for further progress in biometrical objectives. A phylogenetic tree, derived using Nei's coefficient of similarity revealed the different pattern of genetic distance between lines.

한국산 박새속(참새목, 박새과) 조류의 계통진화 (Systematic and Evolutionary Study on the Genus Parus(Passeriformes : Paridae) in Korea)

  • 박병상;현재범;양서영
    • Animal Systematics, Evolution and Diversity
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    • 제6권1호
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    • pp.17-28
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    • 1990
  • 한국산 박새속(Parus) 6종 및 아종 11개 집단 159개체를 재료로 전기영동을 실시, 이들의 계통분류 및 종분화를 조사하였다. 박새(P. major) 3아종의 평균 유전적 변이정도는 일반적인 조류의 유전적 변이정도와 대체로 유사하였으나 나머지 좋은 변이정도가 낮았다. 박새 3아종의 집단 간 및 아종 간 유전적 근연치는 공히 S=0.95 이상으로 높았으며 이들은 집단간 근연치와 아종 간 근연치가 유사하였다. 종간에는 박새(P. major)와 곤줄박이(P. varius varius)의 유전적 근연치가 S=0.80으로 제일 높았고 쇠박새(P. palustrius hellmayri)와 진박새(P. ater amurensis)가 S=0.67로 제일 낮았다. 4종 평균 유전적 근연치는 S=0.72로 일반적인 조류의 근연치와 유사하였다. 이들의 분화연대를 추정한 결과 쇠박새는 약 180만년 전에 박새속 조류의 공통조상에서 분화되었다고 추정되며 그후 진박새는 약 160만년전, 곤줄박이는 약 100만년 전에 각각 분화되었고 박새 3아종은 약 10만년 전후에 분화되었을 것으로 추정된다.

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A preliminary study of genetic structure and relatedness analysis of Nutria (Myocastor coypus) in Upo Wetland

  • Jung, Jongwoo;Jo, Yeong-Seok
    • Journal of Species Research
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    • 제1권1호
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    • pp.100-103
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    • 2012
  • Nutria Myocastor coypus is one of a well known invasive riparian mammal found species around world from North America to Eurasia and Africa. In South Korea, feral nutrias inhabit areas from the Nakdonggang and Namgang (River) to their tributaries and Upo Wetland where they have had devastating effects on environment. Nevertheless, there has been little research about nutrias in Korea. This study is to analyze the genetic structure of the nutria population in the Upo Wetland and identify the origin of the source populations. Twenty individuals from the Upo Wetland were genotyped using 25 microsatellite loci. When compared with another introduced population, that of the Blackwater Nation Wildlife Refuge in U.S., the Upo population contains considerable genetic variations. Tests for Hardy-Weinberg equilibrium and Bayesian clustering analysis suggest the Upo population is genetically structured and has at least two source populations. This preliminary study presents the need for further in-depth studies about this species which should combine genetic and ecological studies.

DNA Marker Traceability Techniques for Hanwoo Industry

  • Lee, Jea-Young;Bae, Jung-Hwan;Lee, Yoon-Seok;Yeo, Jung-Sou
    • Journal of the Korean Data and Information Science Society
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    • 제17권4호
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    • pp.1041-1051
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    • 2006
  • The effectiveness of genetic markers on Hanwoo traceability systems was applied and evaluated from Korean 33 Hanwoo elite sire families. Five microsatellite markers were selected finally, which were located on chromosomes different chromosomes with the end sequencing of 100 HW-YUBAC that were recorded in the NCBI by Yeungnam University. Eleven major microsatellite markers were selected from allele amplified, their frequencies, H(Heterozygosity) and PIC(Polymorphism information content) with Hardy-Weinberg equilibrium. Next, in order to evaluate the power of the markers selected on the individual animal identification with experimental condition, the match probability(MP) and the relatedness coefficient(R) were computed.

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Hanwoo(Korean Cattle) Traceability Using DNA Markers

  • Yeo, Jung-Sou;Rhee, Sung-Won;Choi, Yu-Mi;Kwon, Jae-Chul;Lee, Jea-Young
    • Communications for Statistical Applications and Methods
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    • 제13권3호
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    • pp.733-743
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    • 2006
  • To apply and evaluate the effectiveness of genetic markers on Hanwoo traceability systems, samples of 33 Hanwoo individuals from Korean elite sire families were used, and five microsatellite markers were selected finally, which were located on chromosomes different chromosomes with the end sequencing of 100 HW-YUBAC that were recorded in the NCBI by Yeungnam University. Ten major microsatellite markers were selected from alleles amplified, their frequencies, H(Heterozygosity) and PIC(Polymorphism information content) with Hardy-Weinberg equilibrium. Next, in order to evaluate the power of the markers selected on the individual animal identification, the match probability(MP) and the relatedness coefficient(R) were computed.

U라인에서의 작업관련성을 고려한 작업부하 평활화에 관한 연구 (A Study on Workload Smoothness Considering Work Relatedness In the U-Line)

  • 김우열;김용주;김동묵
    • 한국국방경영분석학회지
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    • 제28권2호
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    • pp.116-124
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    • 2002
  • In just-in-time production systems, U-shaped production lines rather than traditional straight lines are often adopted since they have some advantages. The advantages of U-lines over straight lines are that the workstations required can be reduced and the necessary number of workers can be easily adjusted when the demand rates are changed. In this paper, we present a new genetic algorithm(GA) to minimize the number of workstations primarily and improve the work relatedness secondarily in the U-line production systems. Also, a new heuristic method is presented according to the work related factors and characteristics of U-line balancing. Some major aspects of the proposed GA are discussed, with emphasis on representation, decoding and evaluation function. Extensive experiments are carried out on well-known test-bed problems in the literature to verify the performance of our algorithm . The computational results show that our algorithm is a promising alternative to existing heuristics.