• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.2
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• pp.131-141
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• 2005

Molecular-based genetic diversity for a set of 141 accessions of Korean barley cultivars and 24 accessions of foreign exotic cultivars were analyzed using random amplified polymorphic DNAs (RAPDs). Different level of genetic variability was observed with 30 random decamer primers in the Korean barley varieties and breeding lines which were preliminarily classified by morphological (hulled & hulless barley) and end-use (malting barley) and/or by the released periods. A total of 74 RAPD bands were scored, and the number of bands per primer varied from 1 to 7 with an average of 2.74. The hulled barley pool had one more marker genotype per primer than the hulless barley pool. The polymorphic information content (PIC) values based on the band pattern frequencies among genotypes varied depending on genetic pools where mean PICs of hulled, hulless and malting barleys were 0.62, 0.57, and 0.43, respectively. Certain genomic loci amplified by opR04, opF01, opB05, and opC13 were highly polymorphic with PIC>0.8. Patterns and temporal trends of genetic diversity assessed over the period from 1970s to 1990s had a tendency to increase, and in particular, this upward slant was quite clear and significant for the hulless barley pool. In the cluster analysis using genetic similarity matrix calculated from RAPD profiles, two major groups and several small subgroups were classified. Major grouping of materials was not affected by the presence of the husk but by their genetic background and the spike-row type. The validity of information on the genetic diversity and relationships between genotypes will have been reviewed to predict their yield potential.

• Biomedical Science Letters
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• v.19 no.1
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• pp.32-40
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• 2013

Pluripotent stem cells (PSCs) have enormous potential in the biomedical sciences because they can grow continuously and differentiate into any kind of cell in the body. However, for future application in regenerative medicine, it is still a challenge to control the differentiation of PSCs without using genetic materials. To control the differentiation of PSCs, small molecules might be the best substitute for genetic materials considering the following advantages: small size, which enables penetration of plasma membrane; easy-to-modify structure; and low chance of genetic recombination in treated cells. Herein, we introduce small molecules that induce the neuroectodermal differentiation of mouse embryonic stem cells (ESCs). The small molecules were identified via ESC-based consecutive screenings of small-molecule libraries composed of 324 natural compounds or 93 selected drugs. The natural compounds discovered in the first screening were used to select 93 structurally similar drugs out of 1,200 approved drugs. In the second screening, among the 93 compounds, we found 4 drugs that induced the neuroectodermal differentiation of ESCs. These drugs were progesteroneor corticoid-derivatives. Our results suggest that small molecules targeting the progesterone receptor or glucocorticoid receptor could be used as chemical tools to induce the differentiation of PSCs into a specific germ lineage.

• Proceedings of the Korean Society For Composite Materials Conference
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• 2002.10a
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• pp.233-236
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• 2002

An efficient method was developed in this study to obtain optimal stacking sequences, thicknesses, and minimum weights of stiffened laminated composite shells under combined loading conditions and stiffener layouts using genetic algorithms (GAs) and finite element analyses. Among many parameters in designing composite laminates determining a optimal stacking sequence that may be formulated as an integer programming problem is a primary concern. Of many optimization algorithms, GAs are powerful methodology for the problem with discrete variables. In this paper the optimal stacking sequence was determined, which gives the maximum critical buckling load factor and the minimum weight as well. To solve this problem, both the finite element analysis by ABAQUS and the GA-based optimization procedure have been implemented together with an interface code. Throughout many parametric studies using this analysis tool, the influences of stiffener sizes and three different types of stiffener layouts on the stacking sequence changes were throughly investigated subjected to various combined loading conditions.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.17 no.5
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• pp.131-136
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• 2017

Recently, with the development of Internet media, many document materials have become exponentially increasing on the web. These materials are described, and the information on what is the most by this text are classified according. However, the text has meant that many have room for ambiguous interpretation must look at it from various angles in order to interpret them correctly. In conventional classification methods it was simply a classification only have the appearance of the text. In this paper, we analyze it in terms genetic algorithm and local preserving based techniques and implemented a clustering system fragmentation them. Finally, the performance of this paper was evaluated based on the implementation results compared to traditional methods.

• Journal of the Semiconductor & Display Technology
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• v.20 no.4
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• pp.16-22
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• 2021

We search for an appropriate initial adsorption coordination of precursor on surface by using genetic algorithm (GA) and density functional theory. SiH₄ and Al(CH₃)₃ as precursor, and OH-terminated Si (001) as surface are used for this study. Selection, crossover, and mutation as hyperparameters of GA are applied to search for the adsorption coordination of the precursors on the surface as a function of generation. Bond distances between precursors and the surface are used to explain the adsorption behavior of the precursors.

• Journal of Marine Bioscience and Biotechnology
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• v.1 no.2
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• pp.59-62
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• 2006

Seaweed biotechnology is a multidisciplinary subject to produce food, pharmaceuticals, chemicals, and environmental remediation materials from seaweed resources. It uses various techniques of cell culture, enzyme reaction and genetic manipulation to increase the production efficiency of useful seaweeds or their products. Firstly, an overview of key topics will be introduced in the fields of seaweed tissue culture, strain improvement, genetic analysis briefly as basic techniques. Secondly, some biologically active substances such as anti-inflammatory and antifouling substances that have been screened in my laboratory will be focused.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.85-85
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• 2017

Potato is important carbohydrate source over the world in that revealing high productivity per the unit area, and their cultivation area is estimated to be increased to cope with a scarcity of food according to the population increase. Major cultivated species of potato is Solanum tuberosum (2n = 4x = 48) and regarded as being originated in Andes region of South America. The diverse potato genetic resources has been collected and perserved in Highland Agricultural Research Institute (NICS, RDA), and the genetic materials as DNA stock is conserved in National Agrobiodiversity Center(NAS, RDA). The understanding of genetic constitution of conserved diversity is the basis for the germplam management and further utilization. In this study, we analyzed the genetic diversity of potato germplasm(479 accessions) using 24 microsatellite markers which have been internationally used for fingerprinting of potato accession. The allele number and polymorphic information content (PIC) of total accessions per locus was ranged from 2 to 18 (mean = 8.2) and from 0.214 to 0.771 (mean = 0.595), respectively. Especially, the accession originated from Korea revealed average allele number of 6.0 (2 - 11) and average PIC value of 0.58 (0.193 - 0.763). Three groups were deduced by phylogenic analysis (Group-1, -2, -3); Korean accessions showed close genetic similarity to Japanese and USA accessions, and Korean landraces were mainly included in Group-3. We try to elaborate the genetic diversity analysis of conserved potato germplasm by acquiring more genotypes using applicable molecular markers.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.10a
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• pp.149-150
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• 2003

The purpose of this experiment is to optimize the yield of the recombinant Cox2 from the stably transformed Drosophila melanogaster S2 cells, using dimethylsulfoxide and sodium butyrale. Materials and Methods : Materials - Cell line : Drosophila melanogaster Schneider 2 (S2) cells - vector pMT/BiP/V5-His and pCoHygro (Invitrogen) (omitted)

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.10a
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• pp.147-148
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• 2003

The purpose of this experiment is to confirm whether the recombinant tumstatin revealed from the stably transformed Drosophila melanogaster S2 cells has in vitro capacity. Materials and Methods : Materials - Cell line : Drosophila melanogaster Schneider 2 (S2) cells - vector pMT/BiP/V5-His and pCoHygro (Invitrogen) (omitted)

• Proceedings of the Korean Society For Composite Materials Conference
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• 2001.05a
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• pp.9-12
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• 2001

An active control of the vibration transmitted by longitudinal load in flight control system is investigated numerically. The flight control system is modeled as a finite, thin shell cylinder with constant thickness. A vibration source is generated by exterior monopole source. Distributed piezoelectric actuator is used to control of the vibration. Thin shell theory is used to formulate the numerical models. The amplitude of vibration at discrete location and power transmission are minimized by analytical optimization method. Genetic algorithm is used as numerical optimization method to search optimal actuator position and size which amplitude of vibration is minimized.