Dimethylsulfoxide and Sodium Butyrate Enhance the Production of Recombinant Cyclooxygenase 2 in Stably Transformed Drosophila melanogaster S2 Cells

  • Lee, Jong-Min (Department of Genetic Engineering, Kyung Hee University) ;
  • Sohn, Bong-Hee (Department of Sericulture & Entomology, National Institute of Agricultural Science & Technology, RD) ;
  • Kim, Yong-Soon (Department of Sericulture & Entomology, National Institute of Agricultural Science & Technology, RD) ;
  • Kang, Pil-Don (Department of Sericulture & Entomology, National Institute of Agricultural Science & Technology, RD) ;
  • Lee, Sang-Uk (Department of Sericulture & Entomology, National Institute of Agricultural Science & Technology, RD) ;
  • Chung, In-Sik (Department of Genetic Engineering, Kyung Hee University)
  • Published : 2003.10.01

Abstract

The purpose of this experiment is to optimize the yield of the recombinant Cox2 from the stably transformed Drosophila melanogaster S2 cells, using dimethylsulfoxide and sodium butyrale. Materials and Methods : Materials - Cell line : Drosophila melanogaster Schneider 2 (S2) cells - vector pMT/BiP/V5-His and pCoHygro (Invitrogen) (omitted)

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