• Journal of Microbiology
• /
• 제35권4호
• /
• pp.341-346
• /
• 1997

In order to express recombinant porcine TGF-${\beta}1$ protein in a baculovirus expression system the entire TGF-${\beta}1$gene containing extra amino acids at the N terminus was cloned into pFBa and pFBb of the Bac-To-$Bac^{TM}$ baculovirus expression system. One of the clones contained 106 extra amino acids and was designated pFBa-106 TGF-${\beta}1$, and the other had 28 extra amino acids and was designated pFBb-28 TGF-${\beta}1$. The orientation of the gene was identified with restriction enzyme mapping and PCR with internal TGF-${\beta}1$ primers. Sf-9 cells were infected at a m.o.i. of 10 by the recombinant viruses generated from the two expected sizes of 55 kD and 46.4kD. these precursor forms of TGF-${\beta}1$ with a polyclonal antibody against human TGF-${\beta}1$. No mature form of TGF-${\beta}1$ protein was detected on SDS gels and an immunoblot indicated that TGF-${\beta}1$ precursor is not properlu processed in insect cells.

• Journal of Microbiology
• /
• 제35권4호
• /
• pp.264-270
• /
• 1997

The structure of plasmid mlp1, a linear 10.2kb mitochondrial plasmid of Pleurotus ostreatus NFF A2 was determined by restriction enzyme mapping and partial sequencing. The plasmid encodes at least two proteins; a putative RNA polymerase showing homology to yeast mitochondrial RNA polymerase and to viral-encoded RNA polymerases, and a putative DNA polymerase showing significant homology to the family B thpe DNA polymerases. It also contains terminal inverted repeat sequences at both ends which are longer than 274 bp. A 1.6 kb EcoRI restriction fragment of m1p1 containing the putative RNA polymerase gene did not hybridize to the nuclear or motochondrial genomes from P. ostreatus, suggesting that it may encode plasmidspecific RNA polymerase. The gene fragment also did not hybridize with the RNA polymerase gene (RPO41) from Saccaromyces cerevisiae. The relationship between genes in m1p1 and those in another linear plasmid pC1K1 of Claviceps purpurea was examined by DNA hybridization. The result indicates that the genes for DNA and RNA polymerases are not closely related with those in C. purpurea.

• Asian-Australasian Journal of Animal Sciences
• /
• 제18권11호
• /
• pp.1524-1528
• /
• 2005

Molecular genetic markers were used to detect chromosomal regions which contain economically important traits such as growth, carcass, and meat quality traits in pigs. A three generation resource population was constructed from a cross between Korean native boars and Landrace sows. A total of 240 F2 animals from intercross of F1 was produced. Phenotypic data on 17 traits, birth weight, body weights at 3, 5, 12, and 30 weeks of age, teat number, carcass weight, backfat thickness, body fat, backbone number, muscle pH, meat color, drip loss, cooking loss, water holding capacity, shear force, and intramuscular fat content were collected for F2 animals. Animals including grandparents (F0), parents (F1), and offspring (F2) were genotyped for 80 microsatellite markers covering from chromosome 1 to 10. Least squares regression interval mapping was used for quantitative trait loci (QTL) identification. Significance thresholds were determined by permutation tests. A total of 10 QTL were detected at 5% chromosome-wide significance levels for growth traits on SSCs 2, 4, 5, 6, and 8.

• Asian-Australasian Journal of Animal Sciences
• /
• 제21권5호
• /
• pp.624-627
• /
• 2008

An enrichment library of GATA-repeats from genomic DNA was constructed in this study to isolate and characterize microsatellite loci in Tsaiya duck (Anas platyrhynchos). Thirty-three microsatellite markers were developed and used to detect polymorphisms in 30 Tsaiya ducks. A total of 177 alleles were observed and all loci except APT022 were polymorphic. The number of alleles ranged from 2 to 9 with an average of 5.5 per microsatellite locus. The observed and expected heterozygosity of these polymorphic markers ranged from 0.07 to 0.93 with an average number of 0.60 and 0.10 to 0.86 with an average number of 0.61, respectively. Among the polymorphic markers, the observed heterozygosities of 23 loci were higher than 0.50 (69.70%). The polymorphism information content (PIC) in the 32 loci ranged from 0.09 to 0.83 with an average of 0.57. Seven of the 33 duck microsatellite loci had orthologs in the chicken genome, but only APT004 had a similar core repeat to chickens. These microsatellite markers will be useful in constructing a genetic linkage map for the duck and a comparative mapping with the chicken can also provide a valuable tool for studies related to biodiversity and population genetics in this duck species.

• Journal of Plant Biotechnology
• /
• 제44권4호
• /
• pp.349-355
• /
• 2017

The amino acid composition of rice is a major concern of rice breeders because amino acids are among the most important nutrient components in rice. In this study, a genetic map was constructed with a population of 134 recombinant inbred lines (RILs) from a cross between Dasanbyeo (Tongil-type indica) and TR22183 (temperate japonica), as a means to detect the main and epistatic effect quantitative trait loci (QTLs) for the amino acid content (AAC). Using a linkage map which covered a total of 1458 cM based on 239 molecular marker loci, a total of six main-effect QTLs (M-QTLs) was identified for the content of six amino acids that were mapped onto chromosome 3. For all the M-QTLs, the TR22183 allele increased the trait values. The QTL cluster (flanked by id3015453 and id3016090) on chromosome 3 was associated with the content of five amino acids. The phenotypic variation, explained by the individual QTLs located in this cluster, ranged from 10.2 to 12.4%. In addition, 26 epistatic QTLs (Ep-QTLs) were detected and the 25 loci involved in this interaction were distributed on all nine chromosomes. Both the M-QTLs and Ep-QTLs detected in this study will be useful in breeding programs which target the development of rice with improved amino acid composition.

• Asian-Australasian Journal of Animal Sciences
• /
• 제28권11호
• /
• pp.1525-1531
• /
• 2015

Using next-generation sequencing, we conducted a genome-wide scan of selective sweeps associated with selection toward genetic improvement in Thoroughbreds. We investigated potential phenotypic consequence of putative candidate loci by candidate gene association mapping for the finishing time in 240 Thoroughbred horses. We found a significant association with the trait for Ral GApase alpha 2 (RALGAP2) that regulates a variety of cellular processes of signal trafficking. Neighboring genes around RALGAP2 included insulinoma-associated 1 (INSM1), pallid (PLDN), and Ras and Rab interactor 2 (RIN2) genes have similar roles in signal trafficking, suggesting that a co-evolving gene cluster located on the chromosome 22 is under strong artificial selection in racehorses.

• 한국정보과학회논문지:시스템및이론
• /
• 제35권3호
• /
• pp.120-132
• /
• 2008

네트워크-온-칩은 미래 시스템-온-칩 제품을 위한 실용적인 개발 플랫폼으로서 부각되고 있다. 우리는 전압 조절이 가능한 회선을 가진 네트워크-온-칩에서 태스크간 통신으로 인한 전력 소모를 최소화하기 위한 정적 알고리즘을 제시한다. 최적의 통신 속도를 찾기 위해 제시된 (유전자 알고리즘에 기반한) 기법은 네트워크 망 구조, 태스크 할당, 타일 매핑, 라우팅 경로 할당, 태스크 스케줄링과 회선 속도할당을 포함한다. 제시된 설계 기법은 기존의 기법과 비교하여 평균 28%까지 전력 소비를 감소시킬 수 있다는 것을 실험 결과는 보여 준다.

• 한국컴퓨터그래픽스학회논문지
• /
• 제4권2호
• /
• pp.1-13
• /
• 1998

캐릭터 애니메이션의 가장 중요한 목표는 효율적으로 캐릭터의 동작을 제어하는 것이다. 지금까지 인간 보행 애니메이션을 위한 많은 기법들이 제안되었고, 파라미터 보간이나 동작 데이터 매핑 을 사용하여 지친 걸음이나 활기찬 걸음과 같은 보행의 감정을 표현하는 기법도 제안되었다. 본 논문은 하나의 다리를 가진 평면 뜀뛰기 모델에 기반한 인간 주행 모델을 제시한다. 본 논문의 기법은 동작 최적화를 위해 유전자 프로그래밍을 사용하며 다양한 캐릭터 모델에 쉽게 적용될 수 있다. 본 논문은 에너지 최소화 기법을 확장하여 감정 표현을 위한 파라미터 설정에 따라 다양한 동작을 생성할 수 있도록 한다.

• 제어로봇시스템학회논문지
• /
• 제6권3호
• /
• pp.273-283
• /
• 2000

In this paper we suggest an optimal design method of Fuzzy-Neural Networks(FNN) model for complex and nonlinear systems. The FNNs use the simplified inference as fuzzy inference method and Error Back Propagation Algorithm as learning rule. And we use a HCM(Hard C-Means) Clustering Algorithm to find initial parameters of the membership function. The parameters such as parameters of membership functions learning rates and momentum weighted value is proposed to achieve a sound balance between approximation and generalization abilities of the model. According to selection and adjustment of a weighting factor of an aggregate objective function which depends on the number of data and a certain degree of nonlinearity (distribution of I/O data we show that it is available and effective to design and optimal FNN model structure with a mutual balance and dependency between approximation and generalization abilities. This methodology sheds light on the role and impact of different parameters of the model on its performance (especially the mapping and predicting capabilities of the rule based computing). To evaluate the performance of the proposed model we use the time series data for gas furnace the data of sewage treatment process and traffic route choice process.

• The Plant Pathology Journal
• /
• 제19권3호
• /
• pp.159-161
• /
• 2003

The coat protein (CP) gene of Apple mosaic virus (ApMV), a member of the genus Ilarvirus, was selected for the design of virus-specific primers for amplification and molecular detection of the virus in cultivated apple. A combined assay of reverse transcription and polymerase chain reaction (RT-PCR) was performed with a single pair of ApMV-specific primers and crude nucleic acid extracts from virus-infected apple for rapid detection of the virus. The PCR product was verified by restriction mapping analysis and by sequence determination. The lowest concentration of template viral RNA required for detection was 100 fg. This indicates that the RT-PCR for detection of the virus is a 10$^3$times more sensitive, reproducible and time-saving method than the enzyme-linked immunosorbent assay. The specificity of the primers was verified using other unrelated viral RNAs. No PCR product was observed when Cucumber mosaic virus (Cucumovirus) or a crude extract of healthy apple was used as a template in RT-PCR with the same primers. The PCR product (669 bp) of the CP gene of the virus was cloned into the plasmid vector and result-ant recombinant (pAPCP1) was selected for molecule of apple transformation to breed virus-resistant transgenic apple plants as the next step. This method can be useful for early stage screening of in vitro plantlet and genetic resources of resistant cultivar of apple plants.