• Korean Journal of Soil Science and Fertilizer
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• v.49 no.5
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• pp.456-460
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• 2016

Biofertilizers are increasingly available in the market as one of the alternatives to chemical fertilizers. The supply of a high number of viable microorganisms is important for farmers. Lysobacter capsici YS1215 producing chitinases and gelatinases, isolated from soil in Korea, was evaluated for the establishment of an optimal medium condition of its shelf life under an in vitro condition. In this study, the population density of a biofertilizer (L. capsici YS1215) in media containing crab shell and gelatin powder (M1, M2, M3 and M4) was observed to be higher than that of populations grown in TSB (Tryptic soy broth) media (M5, M6 and M7) during experimental period. In addition, the population density at 11 months was over $10^6\;CFU\;mL^{-1}$ in M1, M3 and M4, but under $10^6\;CFU\;mL^{-1}$ in M2, M5, M6 and M7. The best optimal medium for the shelf life was M1 ($2.6{\times}10^6\;CFU\;mL^{-1}$) containing both chitinous and gelatinous materials at 11 months. Therefore, this study provided results of the appropriate medium composition for the enhancement of the shelf life of L. capsici YS1215.

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.4
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• pp.299-305
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• 2014

In this paper, fractional factorial screening design (FFSD) and central composition design (CCD) were used to optimize the medium components for producing chitinase and gelatinase by Lysobacter capsici YS1215. Crab shell powder, nutrient broth and gelatin were proved to have significant effects on chitinase and gelatinase activity by FFSD first. An optimal medium was obtained by using a three factor CCD, which consisted of nutrient broth of $2.0gL^{-1}$, crab shell powder of $2.0gL^{-1}$ and gelatin of $1.0gL^{-1}$, respectively with the highest chitinase activity ($3.34UmL^{-1}$) and gelatinase activity ($14.15UmL^{-1}$). This value was 3.76 and 1.11 fold of the chitinase and gelatinase activity, respectively, compared to the lowest productive medium in the design matrix. In investigating potential of these enzymes partially purified from L. capsici YS1215 for biotechnological use, the crude enzymes was found to be inhibition against pathogenic fungal mycelia: Colletotrichum gleosporioides, Phytophthora capsici, and Rhizoctonia solani. In this study, we demonstrated the optimal medium for producing the chitinolytic and gelatinolytic enzymes by the strain YS1215 and the role of their enzymes that may be useful for further development of a biotechnological use and agricultural use for biological control of phytopathogenic fungi.

• Korean journal of food and cookery science
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• v.26 no.4
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• pp.481-489
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• 2010

This study was conducted to identify the optimal mixing ratios of turmeric powder or beet powder for the production of jelly. To establish the amount of turmeric powder or beet powder that could be added to jelly, physicochemical sensory characteristics and textural properties were measured. Specifically, jellies were prepared using gelatin containing turmeric powder or beet powder at ratios of 0.5, 1, 1.5 and 2%(w/w). Sensory evaluation of color, appearance, sweetness, chewiness, springiness, hardness, transparency and overall acceptability of jelly prepared using 0.5% turmeric powder resulted in a high score. Similarly, the color, appearance, sweetness, chewiness, springiness, transparency and overall acceptability of jelly prepared using 1% beet powder received a high score. Taken together, the results of this study suggest that turmeric and beet can be useful in the production of high quality jelly.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.2
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• pp.162-168
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• 2012

An nematophagous fungi Arthrobotrys thaumasia Nema-1 and Bacillus subtilis C-9, which degrade the collagen and gelatin, were isolated from horticulture plantation soil in Kyungpook Sungju-gun Seonnam-myun and Chungnam Gongju-gun Woosung-myun to develop biological nematode pesticide. When $5,000mg\;kg^{-1}$ of A. thaumasia Nema-1 nematicide powder ($7.0{\times}10^3cfu\;g^{-1}$) was treated to pot including Meloidogyne incognita, the number of nematode's egg mass, which is a index of nematicidal activity, decreased to 35% compared to control. While the number of nematode's egg mass decreased to 67% by treating the nematicide powder mixture of $5,000mg\;kg^{-1}$ Nema-1 and B. subtilis C-9 ($8.5{\times}10^5cfu\;g^{-1}$). Furthermore the number of nematode's egg mass of the mixture containing cinnamon extract $10mg\;kg^{-1}$, each $5,000mg\;kg^{-1}$ of Nema-1 and C-9 nematicide powder was decreased to 84%, comparing to the result showed the number of nematode's egg mass decreased to 24%, by the treatment of chemical nemato pesticide Fosthiazate $24mg\;kg^{-1}$. These results suggested the mixture of microorganisms and plant extract was more effective biological nematicide than the case of only microorganism or plant extract for nematode control.

• Journal of the Korean Professional Engineers Association
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• v.16 no.3
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• pp.5-23
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• 1983

On the Seoul Metropolitan Subway Construction of No. 3, 4 Line, the total length is 57 Km and it is now undergoing almost 55％ progress. The working method is classified into Open Cut of 70% and the rest of 30% tunnelling method in the 48 job site. Above tunnelling method is execute by American Steel Support System and the rest of 10 job site carried out by New Austria Tunnelling Method. This paper describes Blasting Standardizations works on the above Tunnelling ' Open Cut Method under big slogan, first safety, second execution. As a superintendent, I strived standardization of works with Better powder, Better Drills ' Better Pattern. Geological structure of Seoul area is composed by Jurassic Granite and also the above rockgroup are over burden by Alluviums as a Unconformity. First of all, I carried out the standard amount of powder and burden through experimental standard blasting by each powder as following Blasting works in the subway construction is surrounding shop Building, under pass the city river and also under pass highest building basement floor. I made allowable Blasting Vibration Value by West-Germany Vornorm DIN 4150, Teil 3 and should measure each blasting works as fellows all of powder is used basically Low-Gravity and Low Velocity such as Slurry, Ammonium Nitrate ' Finex I, II. for Smooth Blasting Instead of Gelatin Dynamite. Electric Detonation Cap is used basically M/S Delay Cup instead of Electric delay ' Simultaneous cap. I applied following formula V=KW3/4 $D^{-2}$ V=Particle Velocity (Cm/sec) K=Ginh Huh's Value W=Delay Charge (Kg) D=Distance(m) In the Open Cut, within 1m distance from H-pile I made to use the Concrete breaker, as following V=7W/$^{0.5}$V/$^{-1.75}$ On the Concentrate Building area, I advise to use Light class drill ø36m Bit and advance 1.1m per round blasting the three boom jumbo drill over ø45mm used only suburb of city.e Light class drill ø36m Bit and advance 1.1m per round blasting the three boom jumbo drill over ø45mm used only suburb of city.

• Korean Journal of Materials Research
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• v.19 no.6
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• pp.330-336
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• 2009

The size of crystallites in mono-dispersed cubic silver bromide grains was measured by applying a powder X-ray diffraction method and Scherrer's equation to grains that were suspended in swollen gelatin layers. In order to evaluate the existence of defects, the measured crystallite size was compared to those measured by using a scanning electron microscope. In the case of the grains prepared by the controlled double jet method, the size of crystallites was equal to the edge length of the grains that had edge lengths smaller than 400 nm. This result proved the usefulness of the above-stated method for measuring the size of crystallites and also evaluating the presence of any crystal defect in each grain. In the case of the grains, which were precipitated in the presence of a sensitizing dye and potassium iodide, the size of crystallites was smaller than the edge's length, indicating the discontinuities in the grains introduced during the precipitation process.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.55 no.4
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• pp.408-416
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• 2022

This study investigated the physicochemical properties and physiological activities of jelly prepared from gelatin, sugar, bokbunja extract, and different amounts (0, 0.5, 1, 2 and 3%) of fermented sea tangle Saccharina japonica powder (FSP). The jelly moisture, pH, and sugar content slightly increased with increasing the FSP content. Hardness, springiness, gumminess, chewiness, and cohesiveness also increased with increasing FSP concentration. Jelly antioxidant activity did not change significantly with increasing FSP. In contrast, alcohol dehydrogenase and aldehyde dehydrogenase activities in the jellies increased significantly with increasing FSP concentration. β-secretase inhibitory activity in jellies also increased with increasing FSP concentration. Jellies containing 0.5 or 1% FSP achieved the highest overall sensory acceptance scores. Taken together, these data indicate that addition of FSP to jelly appears to improve its quality and physiological activities.

• Journal of Korean Neurosurgical Society
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• v.29 no.5
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• pp.664-667
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• 2000

Objective : Excessive bleeding from the exposed cancellous bone surface may cause serious problem such as hematoma formation, infection, transfusion reaction during operation and postoperative period. There are several kinds of bleeding control agent on the cancellous bone surface including bone wax, gelatin sponge ($Gelfoam^{(R)}$), oxidized cellulose($Oxycel^{(R)}$, $Surgicel^{(R)}$), thrombin, microfibrillar collagen($Avitene^{(R)}$) etc. In the past, bone wax was used to control bone bleeding but it is associated with increased infection rate and fusion failure. Recently, gelfoam paste has been used to control cancellous bone bleeding. We controlled the cancellous bone bleeding with the mixture of gelfoam powder and thrombin powder. Material and Methods : Seventeen patients of posterior fusion on the 4 motion segments of thoracolumbar spine were selected to compare the result of bone bleeding control. In the test group of 9 patients, the cancellous bone bleeding was controlled with the mixture of Gelfoam and thrombin powder during operation. In the control group of 8 cases, no chemical hemostatic agent was used to manage the bone bleeding during operation. We calculated the total amount of bleeding from cancellous bone surface during and after operation in the two groups and compared their statistic significance of the result which was judged by student t-test. Results : The average amount of intraoperative bleeding was 1825ml in control group, 811ml in test group(p<0.01). The amount of postoperative bleeding was 943ml in control group and 812ml in test group, there were no significant difference in the amount of bleeding during postoperative period between two groups(p>0.5). Total amount of blood was decreased in as much as 1150ml in test group(p<0.01). Conclusion : We concluded that the application of the mixture of thrombin and gelfoam powder on the cancellous bone surface is the effective control method of cancellous bone bleeding for multilevel posterior spinal fusion.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1997.04a
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• pp.118-118
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• 1997

Solid dispersions were used to increase the dissolution rate of biphenyl dimethyl dicarboxylate (DDB) in water, with the ultimate goal of optimizing its bioavailability when incoporated into pharmaceuticals. Carriers used were Kollidon 30, Kollidon VA 64, 2-hydroxypropyl-${\beta}$-cyclodextrin (HPCD), sodium salicylate or sodium benzoate. DDB solid dispersions were prepared at drug to carrier proportions ranging from 1 : 5 to 1 : 20 (w/w) by solvent evaporation method. DDB tablets (7.5 mg) were prepared by compressing the powder mixture composed of solid dispersions, lactose, corn starch, crospovidone and magnesium stearate using a single-punch press. DDB capsules (7.5 mg) were prepared by filing the mixture into empty hard gelatin capsules (size #1). Dissolution studies of DDB from powdered solid dispersions, tablets and capsules were performed in 900 $m\ell$ of water at 100 rpm and 37$^{\circ}C$ by the paddle method. The dissolved amount was assayed by HPLC and expressed as the mean(%)of three determinations.

• Journal of Conservation Science
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• v.35 no.5
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• pp.385-391
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• 2019

In order to examine the material characteristics of a black and white photograph of the Borobudur temple, in Indonesia, several analyses were conducted. A sample was taken from a black and white photograph; microscopy, Fourier transform-infrared spectroscopy, scanning electron microscopy/energy dispersive spectroscopy, X-ray diffraction, and X-ray fluorescence analyses showed that the black and white photograph was composed of four layers including a paper substrate, baryta, binder and surface layers. It was confirmed that the substrate was paper made of cellulose fiber from Cannabis sativa, the baryta layer was made of barium sulfate(BaSO₄) in powder form, the binder was an emulsion containing silver halide, and the surface protective layer was made of gelatin. Since photographs have different characteristics and require different preservation environments depending on the material of construction, it is necessary to study various black and white photographic material characteristics corresponding to each time period.