• Journal of Plant Biology
• /
• v.15 no.1
• /
• pp.42-42
• /
• 1972

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2003.10a
• /
• pp.131.2-132
• /
• 2003

White rot on garlic caused by Sclerotium cepivorum firstly occurred at Goheoung, Jeonnam in 1998. Thereafter, the disease rapidly spread throughout the country except Gangwon and became a major limiting factor for the cultivation of various Allium species such as garlic, onion, and welsh onion. The disease that has not been reported on a wild garlic(Allium monanthum) previously occurred severely at Seosan, Choongnam in 2003. Among cultivation areas in the region, 10.7％ were infected by the disease and the ratio of diseased plant reached up to 55.0％ in some heavily infected fields. Two species of Sclerotium were consistently isolated from infected samples and identified as S. cepivorum or another Sclerotium sp. Averaged size of sclerotium of the former was 455.0x562.2 urn, while the later was 374.4${\times}$347.2$\mu\textrm{m}$. Patogenicity to Allium species and mycological characteristics such as sclerotium size, growth temperature, and microconidia of the fungi were similar to those reported on other Allium species previously. Consequently, the wild garlic is a newly reported host of the two pathogenic fungi in Korea.

• Research in Plant Disease
• /
• v.9 no.3
• /
• pp.121-124
• /
• 2003

Occurrence of rust on 2 representative garlic cultivars Daeseo and N므애 in major cultivating regions of Goheung-gun, Jeonnam and Namhae-gun, Gyeongnam was investigated during 1998-1999. Rust disease severities of garlic ranging from 1.2 to 85.8 were quite different according to the investigating years and cultivars. Relatively high rust severities of garlic were observed in 1998 compared with 1999. This might be due to remarkably frequent rainfalls and high humidities during the growing seasons of garlic in 1998. Rust occurred severely on cultivar Daeseo but not on cultivar Namdo. This shows quite different susceptibility or resistance to rust among the cultivars of garlic. Increase of rust severities resulted in remarkable decrease in yields of garlic. It is assumed that rust might cause serious damages in the cultivation of garlic.

• Korean Journal of Plant Resources
• /
• v.11 no.3
• /
• pp.264-271
• /
• 1998

This study was conducted to investigate morphological characteristics of Korean local garlic varieties and to define the genetic variability and the correlations amongthe characters associated with yield. The plant height, the leaf sheath diameter, the nubmer of leaves, and bulb weight were significantly increased in the garlic plants from heavier seed bulbs. The proportation of six clove bulbs was more than 60% in Seosan variety and this was not realted with the number of cloves in seed bulb. While more than 70% bulbs in Jeoksung and Danyang varieties cultivated in paddy field were composed of 6 or 7 cloves, majority of the bulbs of Euisung and Danyang varieties cultivated in upland were composed of bulbs with 7 cloves or more. The highest genotypic coefficient of variation (GCV) and phenotypic coefficient of variation (PCV) were observed in bulb weight. GCV and PCV also showed high value in leaf sheath diameter. Thus, there is a greater scope for selection of these characters. The small difference between PCV and GCV values in plant height indicates that the environmental influence would be limited in this character. High heritability was observed of plant height (56.76%) and bulb weight (45.95%). And the weight per bulb (34.24%) exhibited highest genitic advance followed by leaf sheath diameter (18.8%) and plant height (9.61%), and those would be the ideal characters for selelction.

• Korean Journal of Plant Resources
• /
• v.28 no.4
• /
• pp.541-545
• /
• 2015

This study investigated the effect of planting density on growth and yield of elephant garlic. Three planting densities of 20 × 20, 20 × 15, and 20 × 10 ㎝ were tested with the furrow width fixed at 120 ㎝ for the evaluation of elephant galic growth and yield. The average date of emergence was middle and late November, requiring about 30 days for the all emergence. For the flowering, 221 days after sowing were required in all the treatments. Plant height and leaf growth were not significantly different according to the planting density. Flower stalk was shorter when planting density was narrow. The L/D ratio was decreased to form oval shape when planting density was narrow. The yield of elephant garlic was 1,811 ㎏ /10a in planting density 20 × 20 ㎝, 2,375 ㎏/10a in 20 × 15 ㎝, and 2,838 ㎏/10a in 20 × 10 ㎝ plot. The marketable garlic ratio was highest as 1,593 ㎏/10a in planting density of 20 × 15 ㎝.

• Korean Journal of Plant Resources
• /
• v.14 no.2
• /
• pp.139-147
• /
• 2001

This study was conducted to clarify of relationship with major physiological characters and RAPD patterns of garlic(Allium sativum L.) germplasm collected from the worldwide using randomly amplified polymorphic DNA(RAPD) analysis. Eighty-four garlic accessions were classified into ten varietal groups by physiological characters with the single linkage clustering based on Q correlations. The majority was early maturing varieties collected from East-Asia, late maturing varieties were Europe. RAPD marker, $WE61_{1,630}$ was amplified with late maturing varieties and high correlation have shown, though three accessions weren't amplified. Clove undifferentiation and secondary growth had mainly occur accessions collected from Europe, but hadn't shown perfect linkage to RAPD. RAPD marker, $WF70_{1,400}$ appeared in bolting garlic and $WF64_{1,400}$ appeared only in fertile garlic. Unknown garlic amplified in $WF64_{1,400}$ might be fertile garlic, because of their collection site were from Central-Asia.

• Asian-Australasian Journal of Animal Sciences
• /
• v.25 no.6
• /
• pp.806-811
• /
• 2012

This study was conducted to evaluate effects of plant extracts on methanogenesis and rumen microbial diversity in in vitro. Plant extracts (Artemisia princeps var. Orientalis; Wormwood, Allium sativum for. Pekinense; Garlic, Allium cepa; Onion, Zingiber officinale; Ginger, Citrus unshiu; Mandarin orange, Lonicera japonica; Honeysuckle) were obtained from the Plant Extract Bank at Korea Research Institute of Bioscience and Biotechnology. The rumen fluid was collected before morning feeding from a fistulated Holstein cow fed timothy and commercial concentrate (TDN; 73.5%, crude protein; 19%, crude fat; 3%, crude fiber; 12%, crude ash; 10%, Ca; 0.8%, P; 1.2%) in the ratio of 3 to 2. The 30 ml of mixture, comprising McDougall buffer and rumen liquor in the ratio of 4 to 1, was dispensed anaerobically into serum bottles containing 0.3 g of timothy substrate and plant extracts (1% of total volume, respectively) filled with $O_2$-free $N_2$ gas and capped with a rubber stopper. The serum bottles were held in a shaking incubator at $39^{\circ}C$ for 24 h. Total gas production in all plant extracts was higher (p<0.05) than that of the control, and total gas production of ginger extract was highest (p<0.05). The methane emission was highest (p<0.05) at control, but lowest (p<0.05) at garlic extract which was reduced to about 20% of methane emission (40.2 vs 32.5 ml/g DM). Other plant extracts also resulted in a decrease in methane emissions (wormwood; 8%, onion; 16%, ginger; 16.7%, mandarin orange; 12%, honeysuckle; 12.2%). Total VFAs concentration and pH were not influenced by the addition of plant extracts. Acetate to propionate ratios from garlic and ginger extracts addition samples were lower (p<0.05, 3.36 and 3.38 vs 3.53) than that of the control. Real-time PCR indicted that the ciliate-associated methanogen population in all added plant extracts decreased more than that of the control, while the fibrolytic bacteria population increased. In particular, the F. succinogens community in added wormwood, garlic, mandarin orange and honeysuckle extracts increased more than that of the others. The addition of onion extract increased R. albus diversity, while other extracts did not influence the R. albus community. The R. flavefaciens population in added wormwood and garlic extracts decreased, while other extracts increased its abundance compared to the control. In conclusion, the results indicated that the plant extracts used in the experiment could be promising feed additives to decrease methane gas emission from ruminant animals while improving ruminal fermentation.

• The Plant Pathology Journal
• /
• v.40 no.1
• /
• pp.40-47
• /
• 2024

Garlic can be infected by a variety of viruses, but mixed infections with leek yellow stripe virus, onion yellow dwarf virus, and allexiviruses are the most damaging, so an easy, inexpensive on-site method to simultaneously detect at least these three viruses with a certain degree of accuracy is needed to produce virus-free plants. The most common laboratory method for diagnosis is multiplex reverse transcription polymerase chain reaction (RT-PCR). However, allexiviruses are highly diverse even within the same species, making it difficult to design universal PCR primers for all garlic-growing regions in the world. To solve this problem, we developed an inexpensive on-site detection system for the three garlic viruses that uses a commercial mobile PCR device and a compact electrophoresis system with a blue light. In this system, virus-specific bands generated by electrophoresis can be identified by eye in real time because the PCR products are labeled with a fluorescent dye, FITC. Because the electrophoresis step might eventually be replaced with a lateral flow assay (LFA), we also demonstrated that a uniplex LFA can be used for virus detection; however, multiplexing and a significant cost reduction are needed before it can be used for on-site detection.

• Journal of Plant Biotechnology
• /
• v.5 no.2
• /
• pp.95-99
• /
• 2003

The leaf segments of garlic (Allium sativum L.) were cultured in vitro and determined optimal concentration of plant growth regulators and sugars for callus induction, multiple shoots regeneration and in vitro bulblet formation. Highest yield of callus was observed in the leaf segment culture on Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4-D, 30 g/L sucrose and 8 g/L agar. Regeneration rate of multiple shoots from callus was high in the MS medium supplemented with kinetin 3.0 + NAA 3.0 mg/L or SA 1.0 + NAA 3.0 mg/L, containing 30 g/L sucrose. High rate of bulblet formation was observed as the concentration of jasmonic acid increased from 0.5 to 2.0 mg/L in medium, whereas addition of gibberellic acid significantly suppressed bulblet formation. The rate of in vitro bulbing was as high as $96\%$ in MS medium supplemented with 2.0 mg/L jasmonic acid and 120 g/l sucrose after two month culture at $25{\pm}1^{\circ}C$ under 16 hours day light.

• Research in Plant Disease
• /
• v.11 no.1
• /
• pp.28-34
• /
• 2005

This study was conducted to investigate the control possibility of garlic white rot causing severe yield losses of Allium species and cultivars using cultural practices such as optimal sowing date and burial depth, and lime application. Inoculum density in infested field soil was investigated at different soil depth, and that on the diseased plant debris was done. Inoculum density and recovery ratio of white rot pathogen of garlic was highly different between two species of Sclerotium cepivorum forming comparatively small sclerotia and Sclerotium sp. forming comparatively large ones. It was confirmed that S. cepivorum formed more sclerotia on bulbs of garlic than S. sp., and sclerotial recovery of S. cepivorum was higher than that of S. sp. Inoculum density of white rot pathogen in the infested field at garlic seeding period ranged from one to thirteen sclerotia per 30 g soil. Inoculum density of white rot pathogen decreased remarkably with increasing soil depth and above 95% of sclerotia were distributed within 5 cm of soil depth. Disease severity of white rot was higher on slightly planted garlics than deeply-planted ones. Garlic seed bulbs infected by white rot pathogens were confirmed to be one of main inoculum sources of white rot in the field and the disease incidences caused by garlic seed transmission showed big differences among garlic varieties. When nine garlic varieties harvested from infested plots were sown in the field, highly susceptible varieties, ‘Wando’, ‘Daeseo’, ‘Namdo’ and ‘Kodang’ showed high disease incidences, whereas other five varieties were not infected at all. It was confirmed that white rot occurred higher on early-sown garlics, before middle October, than on late-sown ones, after late October. Meanwhile, increasing application rate of lime ranged from 100 to 300 g reduced disease severity of white rot.