• Title/Summary/Keyword: gamma irradiation

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An ESR Study of Amino Acid and Protein Free Radicals in Solution Part Ⅴ. an ESR Study of Gamma-Irradiated Lysozyme in Frozen Aqueous Solutions

  • Sun Joo Hong;L. H. Pitte
    • Journal of the Korean Chemical Society
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    • v.16 no.1
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    • pp.40-45
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    • 1972
  • An electron spin resonance study has been made on lysozyme in. frozen aqueous solutions irradiated with $_{60}Co$ r-rays in air at $77^{circ}K.$ Water resonances are dominant when the concentration and the temperature are both below 20% and $130^{circ}K$ respectively. More solute radicals are produced in the solution of higher concentration. Majority of the solute radicals results from direct hit of the radiation. The same types of radicals are induced at $77^{circ}K$ whether the substances are irradiated in the dry state or in frozen aqueous solution. Based on these results, it is assumed that the number of ESR centers produced by the secondary intermolecular radical reacions and stabilized in aqueous solutions may depend on the concentration of the solution, and the presence of water may facilitate the secondary radical reactions occuring in the solute molecules after heat treatment. Majority of the solute radicals above around $193^{circ}K$ are believed to react with oxygen to form peroxytype radicals. However, when the solution is subiected to heat-treatment at $265^{circ}K$ after irradiation at $195^{circ}K$ the peroxy-type resonance was not observed, suggestin that an appreciable amount of oxygen is condensed into the ice, at $77^{circ}K.$ in addition to the oxygen that has already been dissolved in solution and react with solute free radicals during the process of heat-treatment. When the solution contains $H_2O_2$, no water resonance but $HO_2$, type resonance was observed probably indicating that the radiation-induced OH radicals are trapped in $H_2O_2$ aggregates and react readily with $H_2O_2$ molecules to poroduce $HO_2$ type radicals even at $77^{circ}K.$.

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Toxicity of 30 kGy Irradiated Cereal Powders for Three Months (30 kGy 감마선 조사된 곡류 분말의 아급성 독성평가)

  • Jeon, Young-Eun;Kim, Hye-Mi;Lee, Ju-Woon;Byun, Myung-Woo;Kang, Il-Jun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.10
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    • pp.1264-1270
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    • 2008
  • In order to evaluate their possible subacute toxicity, the male and female of SD rats were given to 30 kGy irradiated cereal powders for three months. During the experimental periods, appearance, behavior, mortality, body weight, organ weight, and food consumption of rats fed the 30 kGy cereal powders were not affected compared to the non-irradiated control. In urine analysis, histopathological examination, hematological as well as in serum biochemical findings, no significant differences were found between the rats administered with non-irradiated control and 30 kGy cereal powders for three months. These results indicate that cereal powders irradiated at 30 kGy did not show any toxic effects under these experimental conditions.

A study on point defects induced with neutron irradiation in silicon wafer (중성자 조사에 의해 생성된 점결함 연구)

  • 김진현;류근걸
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2002.07a
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    • pp.62-66
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    • 2002
  • The conventional floating zone(FZ) crystal and Czochralski(CZ) silicon crystal have resistivity variations longitudinally as well as radially The resistivity variations of the conventional FZ and CZ crystal are not conformed to requirement of dopant distribution for power devices and thyristors. These resistivity variations in conventional cystals limits the reverse breakdown voltage that could be achieved and forced designers of high power diodes and thyristors to compromise the desired current-voltage characteristics. So to produce high Power diodes and thyristors, Neutron Transmutation Doping(NTD) technique is the one method just because NTD silicon provides very homogeneous distribution of doping concentration. This procedure involves the nuclear transmutation of silicon to phosphorus by bombardment of neutron to the crystal according to the reaction $^{30}$ Si(n,${\gamma}$)longrightarrow$^{31}$ Silongrightarrow(2.6 hr)$^{31}$ P+$\beta$$^{[-10]}$ . The radioactive isotope $^{31}$ Si is formed by $^{31}$ Si capturing a neutron, which then decays into the stable $^{31}$ P isotope (i.e., the donor atom), whose distribution is not dependent on the crystal growth parameters. In this research, neutron was irradiated on FZ silicon wafers which had high resistivity(1000~2000 Ω cm), for 26 and 8.3hours for samples of HTS-1 and HTS-2, and 13, 3.2, 2.0 hours for samples of IP-1, IP-2 and IP-3, respectively, to compare resistivity changes due to time differences. The designed resistivities were approached, which were 2.l Ωcm for HTS-1, 7.21 Ω cm for HTS-2, 1.792cm for IP-1, 6.83 Ωcm for IP-2, 9.23 Ωcm for IP-3, respectively. Point defects were investigated with Deep Level Transient Spectroscopy(DLTS). Four different defects were observed at 80K, 125K, 230K, and above 300K.

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Studies of radiosensitivity (6) Radiosensitivity of insect pests (방사선 감수성에 관한 연구 (6) 해충의 방사선감수성)

  • Bai D. H.
    • Korean journal of applied entomology
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    • v.4
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    • pp.67-74
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    • 1965
  • The work on the 'Studies of radiosensitivity' in Korea has been initiated since 1959 by the author at various institutions in home and foreign countries, and more than 20000 of plant materials in 73 varieties and 41 kinds are treated under varied conditions for the studies of radiosensitivity in plants. However, a study of the susceptibility to radiation in insect pests as well as their biological effects has not been previously reported in this country. During the year of 1964 and 1965, near 50000 of insects at the stages of adult, egg, larva and pupa in 14 different kinds are irradiated under acute X and gamma rays to investigate the behavior of growth and lethality after irradiation, and thrir general tolerances to radiations are compared by the kind of insects, stage of growth, radiation sources and exposured dosages. Through the experiments, it is shown that the lethality in most of insects are high as several kilo roentgens above and no significant effects on pupation. emergence, and oviposition in the treated generation at the dose of 3 Kr. below, but in the following generation , the rice stem borer for instance, no oviposition or decreased laying is observed from the dosages at 3 Kr. to 1 Kr., and the hatching was possible from 1.5 Kr. below of irradiated larvae although laying and hatching are seemed in normal. In general, the lethal dose $50\%$ of tested infect materials at the larval stage can he classified some what 60-80 Kr. for Pine caterpillar, Fall webworm, Camphor silk moth and their relatives, at 40-60 Kr. for Gypsy moth, Monley prominent and some relatives and at 20-30 Kr. for House fly, Rice weevil, Pine sawfly etc. Plant parasitic nematodes such as Hirschmannia and Paratylenchus shown very high resistance to radiation and the LD-50 is observed from 100 Kr. or above. A concept to evaluate the radiosensitivity in insects by taxological family line as a practical measure is not answerable yet, but it ran he solved within the near future through the continuing experiments.

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Development of Dose Verification Method for In vivo Dosimetry in External Radiotherapy (방사선치료에서 투과선량을 이용한 체내선량 검증프로그램 개발)

  • Hwang, Ui-Jung;Baek, Tae Seong;Yoon, Myonggeun
    • Progress in Medical Physics
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    • v.25 no.1
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    • pp.23-30
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    • 2014
  • The purpose of this study is to evaluate the developed dose verification program for in vivo dosimetry based on transit dose in radiotherapy. Five intensity modulated radiotherapy (IMRT) plans of lung cancer patients were used in the irradiation of a homogeneous solid water phantom and anthropomorphic phantom. Transit dose distribution was measured using electronic portal imaging device (EPID) and used for the calculation of in vivo dose in patient. The average passing rate compared with treatment planning system based on a gamma index with a 3% dose and a 3 mm distance-to-dose agreement tolerance limit was 95% for the in vivo dose with the homogeneous phantom, but was reduced to 81.8% for the in vivo dose with the anthropomorphic phantom. This feasibility study suggested that transit dose-based in vivo dosimetry can provide information about the actual dose delivery to patients in the treatment room.

A Study on the Neutron Activation Analysis of Noble Metals in the Ancient Coin (고전(古錢)내 귀금속 원소의 중성자 방사화 분석에 관한 연구)

  • Kwon Soo Chun;Chul Lee;Myung-Zoon Czae;Jong Du Lee;Koo Soon Chung
    • Journal of the Korean Chemical Society
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    • v.37 no.11
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    • pp.961-966
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    • 1993
  • The determination of noble metals such as Ir, Au and Ag in the ancient coins has been studied. For the measurement of the activity of $^{192}Ir,\;^{198}Au\;and\;^{110m}Ag$, radiochemical separations including solvent extraction and ion-exchange chromatography were applied to reduce the interference of high energy ${\gamma}$-ray emitted from various radionuclides with long half-life. As a results, $10^{-11}$ g/g level of Ir could be detected and it was found that the three kinds of the detection limits, i.e., critical, detection, quantitative limit, calculated by the method proposed by Currie, were enhanced. Prior to the re-irradiation with neutron, inactive carrier was added in order to determine the recovery yield of Ir in the radiochemical separation. The average recovery yields of Ir, Au and Ag in the 5 coins were 65.3%, 98.5%, 99.5%, respectively.

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Studies of Silyl-Transfer Photochemical Reactions of N-[(Trimethylsilyl)alkyl]saccharins

  • Cho, Dae-Won;Oh, Sun-Wha;Kim, Dong-Uk;Park, Hea-Jung;Xue, Jin-Ying;Yoon, Ung-Chan;Mariano, Patrick S.
    • Bulletin of the Korean Chemical Society
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    • v.31 no.9
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    • pp.2453-2458
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    • 2010
  • Photochemical studies of N-[(trimethylsilyl)alkyl]saccharins were carried out to investigate their photochemical behavior. Depending on the nature of the substrate and the solvent system employed, reactions of these substances can take place by either SET-promoted silyl migration from carbon to either the amide carbonyl or sulfonyl oxygen or by a N-S homolysis route. The results of the current studies show that an azomethine ylide, arising from a SET-promoted silyl migration pathway, is generated in photoreactions of N-[(trimethylsilyl)methyl]saccharin and this intermediate reacts to give various photoproducts depending on the conditions employed. In addition, irradiation of N-[(trimethylsily)ethyl]saccharin produces an excited state that reacts through two pathways, the relative importance is governed by solvent polarity and protic nature. Finally, photoirradiation of N-[(trimethylsilyl)propyl]saccharin in a highly polar solvent system comprised of 35% aqueous MeOH gives rise to formation of a tricyclic pyrrolizidine and saccharin that generated via competitive SET-promoted silyl transfer and $\gamma$-hydrogen abstraction pathways.

Radiolysis of Oxygenated and Deoxygenated Glycylglycylglycine in Aqueous Solution and in the Solid State (酸素의 存在下와 無酸素下에서의 水溶液 및 固體 Glycylglycylglycine의 放射線分解)

  • Kang, Man-Sik
    • The Korean Journal of Zoology
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    • v.13 no.3
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    • pp.75-84
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    • 1970
  • Gamma-radiolyses of oxygenated and deoxygenated glycylglycylclycine in aqueous solution and in the solid state are observed, with special regards to peptied bond rupture for elucidation of radiolytic mechanism of proteins, by means of chromatorgraphic separation of degradation products, spectrophotometric quantitation of carbonyl compounds, micro-titration of amide formation, infrared spectrophptometry, and ultraviolet spectrophotometry for evaluation of radiation damage. Essential difference of peptide bond rupture is observed in solution and in the solid state, being high in the former and negligible in the latter. On the other hand, the presence of and obsence of oxygen in solution during irradiation are not so significant with respect to peptide bond rupture, except the recombination of free-radicals produced in deoxygenated solution. Peptide bond rupture in solution is attributable to the mechanisms proposed by Garrison et al.; dehydrogenation followed by hydrolysis to yield acid amide and carbonyl function as found on the basis of radiolytic products. Peptide bond attack at $\\alpha$-carbon locus might be suggestive for irradiated solid but not significant in view of low degree of peptide bond rupture.

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Genetic improvement of potato plants

  • Suharsono, Sony
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.12-12
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    • 2017
  • Genetic improvement in potato can be carried out through several approaches, as sexual crosses, somatic hybridization, mutation and genetic engineering. Although the approach is different, but the goal is the same, to get a superior cultivar. Mutation and genetic engineering are very interesting methods for genetic improvement of potato plants. Mutation by gamma-ray irradiation have been performed to get some new potato cultivars which are more resistant to disease and have higher productivity. We have carried out a mutation of some potato cultivars and obtained some excellent clones to be potentially released as new superior cultivars. By the mutation method, we have released one potato cultivar for the French fries industry, and we registered one cultivar of potato for chips, and two cultivar for vegetable potatoes. Actually we are doing multi-location trial for three clones to be released as new cultivars. Through genetic engineering, several genes have been introduced into the potato plant, and we obtained several clones of transgenic potato plants. Transgenic potato plants containing FBPase gene encoding for fructose bisphosphatase, have a higher rate of photosynthesis and higher tuber productivity than non-transgenic plants. This result suggests that FBPase plays an important role in increasing the rate of photosynthesis and potato tuber productivity. Some transgenic potatoes containing the Hd3a gene are currently being evaluated for their productivity. Over expression of the Hd3a gene is expected to increase tuber productivity and induce flowering in potatoes. Transgenic potato plants containing MmPMA gene encoding for plasma membrane ATPse are more tolerant to low pH than non-transgenic plants, indicating that plasma membrane ATPase plays an important role in the potato plant tolerance to low pH stress. Transgenic potato plants containing c-lysozyme genes, are highly tolerant of bacterial wilt diseases caused by Ralstonia solanacearum and bacterial soft rot disease caused by Pectobacterium carotovorum. Expression of c-lyzozyme gene plays an important role in increasing the resistance of potato plants to bacterial diseases.

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Genetics, Agronomic, and Molecular Study of Leaflet Mutants in Mungbean(Vigna radiata(L.) Wilczek)

  • Soehendi, Rudy;Chanprame, Sontichai;Toojinda, Theerayut;Ngampongsai, Sumana;Srinives, Peerasak
    • Journal of Crop Science and Biotechnology
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    • v.10 no.3
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    • pp.193-200
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    • 2007
  • Mungbean plants generally have a relatively close canopy, thus a large amount of self-shading can reduce yield due to poor light penetration. Modification of leaflet type can affect leaf canopy and could alter seed yield. Two multiple leaflet mutants were obtained from gamma-ray irradiation and used to study the mode of inheritance related to leaflet types and to evaluate their agronomic features. The cross between large-heptafoliate leaflet with small-pentafoliate leaflet mutants produce all $F_1$ plants with normal trifoliate leaflets. The $F_2$ plants segregated in leaflet size and leaflet number into a 9:3:3:1 ratio of large-trifoliate: large-heptafoliate: small-pentafoliate: small-heptafoliate plants, suggesting that independent loci control leaflet size and leaflet number. Regarding leaflet number, the $F_2$ population can be classified into normal-trifoliate, small-pentafoliate, large-heptafoliate, and small-heptafoliate at the dihybrid ratio of 9:3:3:1. The gene symbols $N_1,n_1$ and $N_2,n_2$ are proposed to represent leaflet number. Since no plant was found with large-pentafoliate leaflets, we hypothesize that the $N_2$ allele expresses pleiotropic effect on both leaflet number and leaflet size. Another possibility is that an additional locus with S and s alleles controls leaflet size and S is tightly linked with $N_2$. The effect of multifoliate leaflet on yield and yield components was evaluated in four mungbean families each with four leaflet isolines under three environments. Averaging across the families and environments, the normal-trifoliate and large-heptafoliate lines gave higher yield than small pentafoliate and heptafoliate ones. These two large leaflet lines also had higher leaf area per plant than the other multifoliate lines. Therefore, the mungbean lines with a greater leaf area, which were likely to intercept more sunlight, gave greater yield. Three AFLP markers that were found to be linked to number of leaflets per leaf, corresponded to the N1 allele of the smallpentafoliate parent.

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