• Journal of the Korean Society of Manufacturing Process Engineers
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• 제18권3호
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• pp.66-73
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• 2019

Recently, FDM-type 3D printer technology has been developed, and efforts have been made to improve the output formability and characteristics further. Through this, 3D printers are used in various fields, and printer technologies are suggested according to usage, such as FDM, SLA, DLP, and SLM. In particular, the FDM method is the most widely used, and the FDM method technology is being developed further. The characteristics of the output are produced by the FDM-type 3D printer, which is determined by various factors, and particularly the perspective of the Inter-Layer Fill Factor, which is the volume ratio of the laminated material that exerts a direct influence. In this study, the Inter-Layer Fill Factor is theoretically obtained by presenting the internal space between each layer according to the laminate thickness as a cross-sectional shape model, and the cross section of the actual laminated sample is compared with the theoretical model through experiments. Then, the equation for the theoretical model is defined, and the strength change according to each condition (tensile strength of material, reduction slope, strength reduction rate, and output strength) is confirmed. In addition, we investigated the influence on the correlation and strength between laminate thickness and the Inter-Layer Fill Factor.

• BMB Reports
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• 제29권2호
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• pp.116-121
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• 1996

In earlier studies, the genes encoding Escherichia coli thioredoxin and Corynebacterium nephridii thioredoxin C-3 were fused via a common restriction site in the nucleotide sequence coding for the active site of the proteins to generate two chimeric thioredoxins, designated E-C3 (N to C-terminal) and C3-E. The hybrid thioredoxins were overexpressed in E. coli from the cloned chimeric thioredoxin genes by a T7 promoter/polymerase system. To investigate the structure-function relationship of thioredoxin, we purified the E-C3 hybrid thioredoxin through ammonium sulfate fractionation, DEAE-cellulose chromatography, and Sephadex G-50 gel filtration. Its purity was examined on SDS-polyacrylamide gel electrophoresis and the molecular weight of the purified E-C3 hybrid thioredoxin was estimated to be 12,000. On native polyacrylamide gels, the purified E-C3 hybrid thioredoxin shows a much lower mobility than E. coli thioredoxin. E-C3 hybrid thioredoxin exhibits a 40-fold lower catalytic efficiency with E. coli thioredoxin reductase than E. coli thioredoxin. It was shown to catalyze the reduction of insulin disulfide by dithiothreitol. The purified E-C3 hybrid thioredoxin was also characterized in other aspects.

• Journal of the Korean Society of Manufacturing Process Engineers
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• 제19권12호
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• pp.14-20
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• 2020

Currently, the automotive market is intensively researching eco-friendly vehicles such as EV vehicles and hydrogen vehicles. Further, research and developments for the future markets such as autonomous vehicles and the connective cars are coped up continuously along with the rising fuel economy regulations and the emission regulations. In this development, various sensors, batteries, and control devices are fused in order to decrease the weight of the vehicle. Moreover, since the fuel economy regulation is an issue, research on the weight reduction of body parts is underway. Therefore, in this work, a study is conducted to obtain the optimal design of the Dash part that separates the engine room and the passenger seat of the vehicle body by combining lightweight materials with high rigidity materials. The optimal design was obtained using the Finite Element Analysis. Further, AL5083 was used as the lightweight material and ASBC1470 was used for high strength materials. The parts made with this combination of materials had strength equivalent to that of the existing steel and the weight was reduced by 10%.

• Advances in aircraft and spacecraft science
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• 제9권4호
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• pp.301-318
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• 2022

This paper presents the free vibration analysis of 3D printed sandwich beams by using high-order theories based on the Carrera Unified Formulation (CUF). In particular, the component-wise (CW) approach is adopted to achieve a high fidelity model of the printed part. The present model has been used to build an accurate database for collecting first natural frequency of the beams, then predicting Young's modulus based on an inverse problem formulation. The database is built from a set of randomly generated material properties of various values of modulus of elasticity. The inverse problem then allows finding the elastic modulus of the input parameters starting from the information on the required set of the output achieved experimentally. The natural frequencies evaluated during the experimental test acquired using a Digital Image Correlation method have been compared with the results obtained by the means of CUF-CW model. The results obtained from the free-vibration analysis of the FDM beams, performed by higher-order one-dimensional models contained in CUF, are compared with ABAQUS results both first five natural frequency and degree of freedoms. The results have shown that the proposed 1D approach can provide 3D accuracy, in terms of free vibration analysis of FDM printed sandwich beams with a significant reduction in the computational costs.

• Korean Journal of Soil Science and Fertilizer
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• 제21권4호
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• pp.426-433
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• 1988

A pot experiment was conducted to find out the effects of application of slacked lime and fused super-phosphate on the lead uptake of upland crops in a lead added soil. Lead concentration of the soils were adjusted to 0, 150, 300mg/kg respectively. The slacked lime was applied at the equivalent amount of lime requirement with extra 150kg/10a, and 2 times for the fused superphosphate. The results obtained were as follows: 1. Lead contents in crops increased in the order: sesame > maize > potato > sweet potato > soybean > green perilla > peanut > red bean. 2. Lead contents in parts of crops were increased in the order; root > stem > leaf > grain. 3. Increasing lead concentration in soils, lead content in the plant was increased and crops yield were decreased. 4. Lead contents in soybean and green perlilla were decreased in slacked lime application treatment. 5. The lead contents in leaf and grain of soybean and green perllila decreased with decreasing in the ratio of Pb/Ca+Mg equivalent in soil. 6. Grain yield were increased in slacked lime, but were decreased in fused superphosphate application treatment. 7. With increasing the soil Pb contents, calcium and phosphate contents were increased in leaf and stem, but calcium was decreased in roots. 8. $1N-NH_4$ OAC soluble Pb contents in soil were 26-50 ppm and 42-70 ppm, respectively, for 150mg/kg and 300mg/kg lead treatments. 9. The soil pH was increased in the order of slacked lime, fused superphosphate and nontreatment.

• Journal of the Institute of Electronics Engineers of Korea SD
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• 제48권10호
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• pp.82-90
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• 2011

Because of the development of Smart phone devices, the demands of high performance FPU(Floating-point Unit) becomes increasing. Therefore, we propose the high-speed single-/double-precision FPU design that includes an elementary add/sub unit and improved multiplier and compare and convert units. The most commonly used add/sub unit is optimized by the parallel rounding unit. The matrix operation is used in complex calculation something like a graphic calculation. We designed the Multiply-Add Fused(MAF) instead of multiplier to calculate the matrix more quickly. The branch instruction that is decided by the compare operation is very frequently used in various programs. We bypassed the result of the compare operation before all the pipeline processes ended to decrease the total execution time. And we included additional convert operations that are added in IEEE754-2008 standard. To verify our RTL designs, we chose four hundred thousand test vectors by weighted random method and simulated each unit. The FPU that was synthesized by Samsung's 45-nm low-power process satisfied the 600-MHz operation frequency. And we confirm a reduction in area by comparing the improved FPU with the existing FPU.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.105-105
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• 2003

In previous reports, pVPSV.IGR2.1 transgenic mouse were described that brain tumor and lymphoma by reason of Vasopressin-SV40 T antigen. In this study, we produced pVPSV.IGR3.6 transgenic mouse that used pVPSV.IGR3.6 vector. Expression of transgene was vary different in transgenic mouse. We obtained 6 transgenic mouse line, moreover they had died at the age of 2-6 weeks without transmitting the transgene to their offspring, and had tumorigenesis on same location with pVPSV.IGR2.1 transgenic mouse. Only a founder mouse was investigated for expression of fusion gene. Here we extended this transgenic approach to the study of tumor progression. From the mouse, we confirmed brain tumor cell, after then cultured for investigate characterization. In this report, we demonstrate that reduction of survival rate in transgenic mouse fused vasopressin gene length, acquisition of brain tumor cell, composition with astrocyte cells and neuronal cells. Finally, cells had no change with increase of passage.

• The Journal of Korean Academy of Prosthodontics
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• 제33권2호
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• pp.358-394
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• 1995

The author studied the actual conditions on the production of dental prosthesis made in laboratories, and also studied interrelationships between dentists and laboratory technicians in both personal and technical aspects. Two hundred-eighty four technicians, work in dental laboratories presently, were surveyed via mail and direct contact during the period from June 1 to June 30 and August 27 to August 28 in 1994 respectively. The obtained results were as follows : 1. Among the respondents, 90.5% we re working in commercial dental laboratories and their laboratories were mainly located in the Seoul area(40.9%, P<0.05). The numbers of employees in these laboratories were less than 10 persons(70.0%, P<0.01), and 75.9% of these laboratories have been in operation less than 15 years. 2. Most laboratory procedures were accomplished according to established disciplines. However, procedures such as die trimming in fixed restorations and the qualifications of the people designing removable partial dentures were not. Other problem areas were boxing of the working cast, the person determining the posterior palatal seal area, selection and arrangement of artificial teeth, occlusion rim correction and laboratory remounting of the processed denture in complete denture restorations. 3. Only half of the requesting dentists could send work authorizations to the laboratories with their work and even so, its contents were quite lacking. Consequently, there must be some standards in writing work authorization. 4. Technicians most desired clean and accurate impressions in fixed and removable dentures, and enough tooth reduction in porcelain fused to metal restorations. 5. For the establishment of better relationships between dentist and dental technician, the respondents desired the establishment of equal footing first(33.5%), and frequent conversations and muture understanding second(25.9%).

• Journal of Microbiology and Biotechnology
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• 제13권6호
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• pp.976-983
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• 2003

Seven Escherichia coli cells defective with aerobic growth were isolated by the insertion of ${\lambda}placMu53$, a hybrid bacteriophage of ${\lambda}$ and Mu, which created a transcriptional fusion to lacZY. These insertion mutant cells were tested on an XG ($5-bromo-4-chloro-3-indolyl-{\beta}-D-galactopyranoside$) medium for anaerobic expression of lacZ by fusion to a promoter. The chromosomal DNA from these strains were digested by EcoRI, and the EcoRI fragments that contained the fused gene and lacZ sequence were identified by Southern hybridization, using lacZ containing plasmid as a probe. The EcoRI fragment from each strain was cloned and sequenced. The sequence data were compared with the GenBank database. The mutated gene of three strains, CYT4, CYT5, and OS11, was found to be identical, and it was nrdAB that encoded ribonucleoside diphosphate reductase. The gene nrdAB was at min 50.5 on the Escherichia coli linkage map and 2,348,084 on the physical map, and is involved in hemAe-related reduction-oxidation reaction. OS6 and OS14 mutant strains had insertion at min 8.3 and the mutated gene was hemB. The hemB encodes 5-aminolevulinate dehydratase or porphobilinogen synthase. The OS3 mutant had insertion in cydB at min 16.6. The cydAB encodes cytochrome d oxidase. In the case of OS1, the fusion was made with sucA, the E1 component of ${\alpha}-ketoglutarate$ dehydrogenase.

• Proceedings of the KSAR Conference
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• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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• pp.44-44
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• 2003

In previous reports, pVPSV.IGR2.1 transgenic mouse were described that brain tumor and lymphoma by reason of Vasopressin-SV40 T antigen. In this study, we produced pVPSV.IGR3.6 transgenic mouse that used pVPSV.IGR3.6 vector. Expression of transgene was vary different in transgenic mouse. We obtained 6 transgenic mouse line, moreover they had died at the age of 2~6 weeks without transmitting the transgene to their offspring, and had tumorigenesis on same location with pVPSV.IGR2.1 transgenic mouse. Only a founder mouse was investigated for expression of fusion gene. Here we extended this transgenic approach to the study of tumor progression. From the mouse, we confirmed brain tumor cell, after then cultured for investigate characterization. In this report, we demonstrate that reduction of survival rate in transgenic mouse fused vasopressin gene length, acquisition of brain tumor cell, composition with astrocyte cells and neuronal cells. Finally, cells had no change with increase of passage.