• Title/Summary/Keyword: fungal population

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Influence of Yeast Fermented Cassava Chip Protein (YEFECAP) and Roughage to Concentrate Ratio on Ruminal Fermentation and Microorganisms Using In vitro Gas Production Technique

  • Polyorach, S.;Wanapat, M.;Cherdthong, A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.1
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    • pp.36-45
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    • 2014
  • The objective of this study was to determine the effects of protein sources and roughage (R) to concentrate (C) ratio on in vitro fermentation parameters using a gas production technique. The experimental design was a $2{\times}5$ factorial arrangement in a completely randomized design (CRD). Factor A was 2 levels of protein sources yeast fermented cassava chip protein (YEFECAP) and soybean meal (SBM) and factor B was 5 levels of roughage to concentrate (R:C) ratio at 80:20, 60:40, 40:60, 20:80, and 0:100, respectively. Rice straw was used as a roughage source. It was found that gas production from the insoluble fraction (b) of YEFECAP supplemented group was significantly higher (p<0.05) than those in SBM supplemented group. Moreover, the intercept value (a), gas production from the insoluble fraction (b), gas production rate constants for the insoluble fraction (c), potential extent of gas production (a+b) and cumulative gas production at 96 h were influenced (p<0.01) by R:C ratio. In addition, protein source had no effect (p>0.05) on ether in vitro digestibility of dry matter (IVDMD) and organic (IVOMD) while R:C ratio affected the IVDMD and IVOMD (p<0.01). Moreover, YEFECAP supplanted group showed a significantly increased (p<0.05) total VFA and $C_3$ while $C_2$, $C_2:C_3$ and $CH_4$ production were decreased when compared with SBM supplemented group. In addition, a decreasing R:C ratio had a significant effect (p<0.05) on increasing total VFA, $C_3$ and $NH_3$-N, but decreasing the $C_2$, $C_2:C_3$ and CH4 production (p<0.01). Furthermore, total bacteria, Fibrobacter succinogenes, Ruminococcus flavefaciens and Ruminococcus albus populations in YEFECAP supplemented group were significantly higher (p<0.05) than those in the SBM supplemented group while fungal zoospores, methanogens and protozoal population remained unchanged (p>0.05) as compared between the two sources of protein. Moreover, fungal zoospores and total bacteria population were significantly increased (p<0.01) while, F. succinogenes, R. flavefaciens, R. albus, methanogens and protozoal population were decreased (p<0.01) with decreasing R:C ratio. In conclusion, YEFECAP has a potential for use as a protein source for improving rumen fermentation efficiency in ruminants.

A Survey on the population of Toxigenic Fungi in Crude Drugs in Seoul (시판 유통중인 한약재에서의 위해성 진균 분포에 관한 연구)

  • Lee Young-Ki;Park Ok-Soon;Oh Young-Hee;Kim Mu-Sang;Kim Young-Soo
    • Journal of environmental and Sanitary engineering
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    • v.20 no.2 s.56
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    • pp.29-32
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    • 2005
  • We just carried out this study in order to obtain an appropriate information of the population of toxigenic fungi in crude drugs in seoul. Results of fungal examination on twenty-three kinds crude drugs were described in this report. In 21 crude drugs, colonies of fungi were possible to identify into 7 genera. Predominant genera of fungi in crude drugs were Aspergillus spp. $(14,\;46.6\%)$, Scopulariospsis spp. $(7,\;23.3\%)$, Penicillium spp., Rhizopus spp., Fusarium spp $(2,\;6.6\%,\;respectively)$ and Phoma sp., Chaetomium sp. $(1,\; 3.3\%,\;respectively)$. Mycotoxin producing fungi like Aspergillus spp., Penicillum spp., and Fusarium spp. were 18 colonies$(60\%)$, totally.

Vegetative Compatibility Groups and Pathogenicity Variation among Isolates of Fusarium oxysporum f.sp. melonis

  • Ahn, Il-Pyung;Lee, Yong-Hwan
    • The Plant Pathology Journal
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    • v.16 no.4
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    • pp.227-230
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    • 2000
  • A total of 90 isolates of Fusarium oxysporum f.sp. melonis, the causal agent of oriental melon (Cucumis melo var. makuwa) wilt, was isolated from symptomatic tissues of oriental melon from 4 provinces in Korea. These isolates were grouped into vegetative compatibility groups (VCGs) by demonstrating heterokaryosis through complementation using nitrate nonutilizing (nit) mutants. No self-incompatibility was observed in any of isolates. All isolates were grouped into 3 VCGs ; A, B, and C. iSOLATES BELONGING TO VCG A and VCG B accounted for 87% and 91% of the fungal population collected in 1991 and 1993, respectively. As the increment of cultivation period in the same field, the proportion of isolates belonging to VCG B increased whereas that of isolates belonging to VCG A decreased. Mean virulence of a total population increased as the increment of cultivation period in the same field. Isolates belonging to VCG B showed the highest increment of virulence. These data suggest that replanting of a host plant in the same field may cause increase of virulence in the pathogens. Furthermore, virulence of F. oxysporum f.sp. melonis isolates is related to the VCGs.

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Exploration of Mycobiota in Cypripedium japonicum, an Endangered Species

  • Cho, Gyeongjun;Gang, Geun-Hye;Jung, Hee-Young;Kwak, Youn-Sig
    • Mycobiology
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    • v.50 no.2
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    • pp.142-149
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    • 2022
  • Orchids live with mycorrhizal fungi in mutualism. This symbiotic relationship plays an essential role in the overall life cycle of orchids from germination, growth, settlement, and reproduction. Among the 1000 species of the orchid, the Korean lady's slipper, Cypripedium japonicum, is known as an endangered species. Currently, only five natural habitats of the Korean lady's slipper remain in South Korea, and the population of Korean lady's slipper in their natural habitat is not increasing. To prevent extinction, this study was designed to understand the fungal community interacting in the rhizosphere of the Korean lady's slipper living in the native and artificial habitats. In-depth analyses were performed to discover the vital mycorrhizal fungi contributing to habitat expansion and cultivation of the endangered orchid species. Our results suggested that Lycoperdon nigrescens contributed most to the increase in natural habitats and Russula violeipes as a characteristic of successful cultivation. And the fungi that helped L. nigrescens and R. violeipes to fit into the rhizosphere community in Korean lady's slipper native place were Paraboeremia selaginellae and Metarhizium anisopliae, respectively. The findings will contribute to restoring and maintaining the endangered orchid population in natural habitats.

A Comparative Study on the Mushroom Populations between Matsutake-Producing and Non-producing Pinus densiflora Stands (송이발생림(松栮發生林)과 미발생림(未發生林)의 버섯분포(分布)에 관(關)한 비교연구(比較硏究))

  • Lee, Kyung Joon;Kim, Yang Sup;Lee, Tae Soo;Kim, Kyo Soo
    • Journal of Korean Society of Forest Science
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    • v.72 no.1
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    • pp.27-31
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    • 1986
  • To compare the fungal distribution of matsutake-producing (Tricholoma matsutake) stand with that of non-producing stand, mushrooms on forest floor were collected in September of 1984 from 28-year-old non-producing and 55-year-old matsutake-producing stands of Pinus densiflora in Kangwon Province. A total of 18 fungal species in 12 genera were collected from a non-producing stand, and all the species except one were putative ectomycorrhizal. Common genera in non-producing stand included Suillus, Russula, Lactarius, Hydnum, and Amanita. From a matsutake-producing stand a total of 30 fungal species in 22 genera were collected, of which 22 species were ectomycorrhizal. Common genera in the producing stand included Hydnum, Tricholoma, Laccaria, and Cantharellus. Common species in both stands were six species only. It is concluded that matsutake-producing stand had more diversified fungal population than non-producing stand, probably because of higher contents of organic matters in soil and better development of understory vegetation through the ageing of the stand.

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A Study on the Foot and Ankle Disease of Shelter-based Homeless People (노숙인 거주 시설의 족부 족관절 실태에 대한 조사)

  • Min, Hak-Jin;Kim, Ki Chun;Kim, Jae Woo
    • Journal of Korean Foot and Ankle Society
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    • v.21 no.4
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    • pp.156-164
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    • 2017
  • Purpose: Foot and ankle disease (FAD) is a frequent cause of morbidity among the homeless population. Various conditions, exacerbated by malnutrition, poor lifestyle habits, psychiatric disorders, physical injuries, poor hygiene, and limited access to healthcare, have been described in this population. The purpose of this study was to investigate the cognition and management status of FAD in shelter based homeless people. Materials and Methods: Fifty-two male and twenty-five female volunteer homeless individuals were recruited from two homeless shelters. Each person completed a questionnaire assessing any presence of pain, pain management, as well as foot and ankle care status. A foot and ankle surgeon examined the physical status of the individual's foot and ankle, including tenderness and instability. A radiologic evaluation was done for 18 male and 11 female homeless people who agreed to participate in this test for the existence osteophyte or joint space narrowing representing osteoarthritis and some reference angles for hallux valgus, flatfoot and cavus foot. Results: Homeless people had higher prevalence of body mass index, diabetes, and smoking than the general population. The most prevalent infectious disease was fungal infection (male 78%, female 68%), with a low compliance of management for FAD. Conclusion: Although most of shelter-based homeless people showed an acceptable foot and ankle management status, the potential risk for FAD development and exacerbation of mild FAD was high.

Studies on the Population of Toxigenic Fungi in Foodstuffs - II. Fungal flora of various kind of Rice-cakes (각종(各種) 식품종(食品中)의 유독성(有毒性) 진균(眞菌)에 관(關)한 연구(硏究) - II. 각종(各種) 떡중(中)의 사상균(絲狀菌) 분포(分布)에 대(對)하여)

  • Koh, Choon-Myung;Choi, Tae-Joo;Lew, Joon
    • The Journal of the Korean Society for Microbiology
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    • v.7 no.1
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    • pp.55-58
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    • 1972
  • The determination of fungi in various kind of rice-cakes have been carried out in order to information of the toxigenic fungi in Korean rice-cakes. The results as follows: 1. Predominant genera of fungi in most rice-cakes were Pencillium sp.(32.2%) and Aspergillus sp.(30.1%). 2. Of the 63 various rice-cakes, 143 strains of fungi were isolated and 135 strains were possible to identify into 9 genera. 3. Artemisia asiatics and some kind of colouring matters have been an antifungal activity.

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Chemical Control of Fusarium Wilt of Pigeonpea (Pigeonpea의 Fusarium 시들음병에 대한 화학적 방제)

  • Singh, Rajesh
    • The Korean Journal of Mycology
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    • v.26 no.4 s.87
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    • pp.416-423
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    • 1998
  • The fungicidal effects of four commercial fungicides, two herbicides and two insecticides have been examined on Fusarium udum, causing wilt disease of pigeonpea in vitro and in vivo. The fungicides Bavistin and MeMc inhibited the growth of the test pathogen completely at 8 and 30 ppm. The herbicide Butachlore inhibited the growth of the test pathogen up to 80.4%. The insecticides, Ekalux and Thiodane partially inhibited the radial growth at 1000 ppm. In unsterilized and sterilized soil MeMc was most effective in controlling the disease in comparison to Bavistin and Ekalux. Maximum rhizosphere fungal population was recorded in MeMc amended soil and minimum in case of Bavistin.

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Variation of Soil Mycoflora in Decomposition of Rice Stubble from Rice-wheat Cropping System

  • Vibha, Vibha;Sinha, Asha
    • Mycobiology
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    • v.35 no.4
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    • pp.191-195
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    • 2007
  • The colonization pattern and extent of decay produced in paddy stubble by soil inhabiting mycoflora were done by using nylon net bag technique. Among the three methods used for isolation of fungi, dilution plate technique recorded the highest number of fungi followed by damp chamber and direct observation method. Nutrient availability and climatic conditions (temperature, humidity and rainfall) influenced the occurrence and colonization pattern of fungi. Maximum fungal population was recorded in October ($48.99{\times}10^4/g$ dry litter) and minimum in May ($11.41{\times}10^4/g$ dry litter). Distribution of Deuteromycetous fungi was more in comparison to Zygomycetes, oomycetes and ascomycetes. In the early stage of decomposition Mucor racemosus, Rhizopus nigricans, Chaetomium globosum and Gliocladium species were found primarly whereas at later stages of decomposition preponderance of Aspergillus candidus, Torula graminis, Cladosporiun cladosporioides and Aspergillus luchuensis was recorded.

Comparative Evaluation of Conidia, Blastospores and Culture Filtrates from Entomopathogenic Fungi against Tetranychus urticae

  • Yun, Hwi-Geon;Kim, Dong-Jun;Lee, Ji-Hoon;Ma, Ji-In;Gwak, Won-Seok;Woo, Soo-Dong
    • International Journal of Industrial Entomology and Biomaterials
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    • v.35 no.1
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    • pp.58-62
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    • 2017
  • The two-spotted spider mite (Tetranychus urticae) has sustained damage on more than 200 host plants worldwide. Many farmers have relied on chemical acaricides to control mite, but the abuse of acaricides has caused serious resistance to mite. To overcome this problem, microbial control using entomopathogenic fungi have been studied. Entomopathogenic fungi have been an important role against the control of pest, and most of their culture products have been demonstrated to have virulence against pest population. In this study, we evaluated and compared the virulence of culture filtrates, aerial conidia and blastospores of selected Metarhizium anisopliae 4-2 and Beauveria bassiana 2R-3-3-1, respectively, among two-spotted spider mite-pathogenic fungi. As a result, the virulence was confirmed in all treatments, and the accumulated mortality rates were between 77 and 100% within 7 days. Especially, treatment with the fungal culture filtrate alone exhibited quite high virulence, and combined treatment with aerial conidia or blastospores enhanced activity. However, the median lethal time of treatments was not significantly different. When two isolates were compared, M. anisopliae 4-2 showed higher virulence than B. bassiana 2R-3-3-1. These results suggest that the selected two fungal isolates and their culture products could be used effectively for the control of two-spotted spider mite.