• 한국약용작물학회지
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• 제26권1호
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• pp.26-31
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• 2018

Background: Angelica gigas, A. sinensis, and A. acutiloba are commercially important in the herbal medicine market, and among them, A. gigas has the highest economic value and price. However, their similar morphological traits are often used for fraud. Despite their importance in herbal medicine, recognition of the differences between Angelica species is currently inadequate. Methods and Results: A multiplex polymerase chain reaction (PCR) method was developed for direct detection and identification of A. gigas, A. sinensis, and A. acutiloba. The gene for the distinction of species was targeted at ITS in the nucleus and trnC-petN gene in chloroplasts. The optimized multiplex PCR in the present study utilized each Angelica species-specific primer pairs. Each primer pair yielded products of 229 base pairs (bp) for A. gigas, 53 bp for A. sinensis, 170 bp for A. acutiloba. Additionally non-specific PCR products were not detected in similar species by species-specific primers. Conclusions: In the present study, a multiplex-PCR assay, successfully assessed the authenticity of Angelica species (A. gigas, A. sinensis, and A. acutiloba). and whole genome amplification (WGA) was performed after DNA extraction to identify, the species in the product. The detection method of raw materials developed in the present study could be applied to herbal medicine and health functional food management.

• 한국한의학연구원논문집
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• 제14권2호
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• pp.27-33
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• 2008

Alimentotherapy refers to the treatment or prevention by means of food. As food is consumed in everyday life and its constituents varies enormously. the practice of alimentotherapy was often misunderstood. By the research on ancient classics we could find 3 prospective about this practice. Firstly, alimentotherapy was used during the recovery and also for prevention. This is a type of Life-nurturing method, which we suggest to be called life-nurturing alimentotherapy. Secondly, it was also used as a dietary prescription made by physicians. Sometimes they made prescriptions combining materials derived from food with herbal medication. This practice was exclusively made by physicians as they had to consider the characteristic of each food as well as the condition of the patient. Thirdly, sometime alimentotherapy was used in cuisine form. They used food components in major part and herbal medicine used as additional ingredients. It was primarily focused on the taste and in some part its functional aspects. The underlying theories in alimentotherapy is exactly the same as used for understanding herbal medicine in TKM. Therefore, except in the case of life-nurturing method, it should be used by expert physicians. Finally, we suggest to differentiate the terms of alimentotherapy in the aspect of life-nurturing, dietary prescriptions and cuisine to prevent confusions.

• 한국식품영양학회지
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• 제21권2호
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• pp.210-217
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• 2008

This study evaluated the hypolipidemic effects of Eleutherococcus senticosus combined with several oriental medicinal herbs. In addition, it addressed whether consuming a diet of healthy food along with a daily supplement of this composite could affect the health status of individuals suffering from diet-related disease such as obesity and hyperlipidemia. The effects of the combined medicinal herbal extracts(MHE) extracted with hot water on reducing serum lipids and improving blood parameters were examined in rats fed a high-fat diet for 5 weeks. Sprague-Dawley rats were randomly assigned to 4 groups: basal diet only(BDG), high fat diet without MHE(FCG), high fat diet and 10% MHE(F10E) and high fat diet and 20% MHE(F20E). Serum lipid contents and blood variables were examined after the experimental period. The results of were as follows. The hematological data for the 4 groups were similar indicating no significant differences. There were moderate level of serum GOT activity in the F10E and F20E groups as compared to the FCG group. Total cholesterol, LDL-cholesterol, triglyceride in serum and the atherogenic index were remarkably reduced in the MHE-supplemented groups as compared to the control group. However, F10E and F20E groups had significantly higher HDL-cholesterol levels than the control group. These results imply that combination of several medicinal herbal extracts could be used to reduce of serum lipid concentrations. The data from this study will be used as basic information with the field of functional food research to address how we can apply oriental medicinal resources to foods. Such research currently drawing considerable attention world-wide.

• 한국식품저장유통학회지
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• 제16권4호
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• pp.506-511
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• 2009

본 연구에서는 기능성 식품인 솔잎쿠키를 만들어 저장기간 중 품질특성을 모니터링 하여 솔잎쿠키의 저장기간에 따른 품질특성 변화을 비교하고자 솔잎가루 첨가량(0%, 3%, 6%, 9%)과 저장일수(1일, 3일, 5일)를 설정하여 색도 및 물성 등을 측정하고 관능검사를 실시하였다. 솔잎첨가량에 따라 명도, 적색도, 황색도(p<0.001), 경도(p<0.05) 에서 유의성이 나타났으며, 저장일수에 따라 명도와 적색도(p<0.01) 에서 유의성이 나타났다. 관능검사 항목 중 솔잎첨가량에 따라 단맛, 텁텁한 정도(p<0.05), 쓴맛, 전체적기호도(p<0.001) 에서 유의성이 나타났다.

• 대한한의학방제학회지
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• 제14권2호
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• pp.97-108
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• 2006

Objective: Functional dyspepsia refers to sustainable or repetitive pain at upper stomach related with food intake. This research was intended to observe the improvement of the symptom by administering Bowhatang to a number of functional dyspepsia patients and to clarify its clinical effects. Method: The research was performed upon the appropriate 36 patients conforming to the criteria after being diagnosed with functional dyspepsia among the 87 patients who visited the department of internal medicine at Gumi Oriental Hospital of Daegu Haany University for digestive diseases and were administered with Bowhatang. Result: 91.8% of the patients had upper stomach globus symptoms. which is of the largest percentage. and 13.5% had hunger pain. which is of the least percentage. After administering Bowhatang. by and large Likert scale was significantly reduced. According to the subjective improvement evaluation by the patients. 32 patients(86%) were improved. and 3 patients(8%) had neither improvement nor relapse. while 2 patients ( 6%) were relapsed. Conclusion: Bowhatang has shown significant improvement about the symptoms for functional dyspepsia patients. It is predicted when more outcomes become available from researches on side effects or toxicity of Bowhatang later on. it will be more often used in the clinic.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.334.2-334.2
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• 2002

As the part of our continuing study for antihyperlipidemic agents from the herbal medicinal resources. we examined the possibility of the ethylacetate fraction of Alpinia officinarum water extract in vitro and in vivo. We isolated some compounds from the ethylacetate fraction of Alpinia officinarum and measured their antihyperlipidemic activities. The active components isolated by silicagel column lmproved serum TG. HDL and LDL level in corn oil feeding and triton WR-1339 induced hyperlipidemic mice. The most active compound was 3-Methylethergalangin. Consequently. these biologically active gerbal functional foods could be used for preparing the healthy food which might diabetas. hyperlipidemia and other disease.

• Journal of Forest and Environmental Science
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• 제26권1호
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• pp.37-51
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• 2010

A mushroom as a functional food material is being increasingly used in these days. It has been that about 1,500 species of mushrooms grow naturally in Asia. Among them about 700 species are edible, but about 30-50 species are poisonous. Even a poisonous mushroom is a very interesting subject for the dietary life research from biochemical viewpoint due to the fact that any mushroom can be the physiological active material. Not a few identified poisonous materials are being used as research reagents thus far. It is suggested that more useful materials could be obtained by progresses in future researches. The identification of the poisonous ingredient can open up a route for mushroom intoxication cure. It is known that cell destruction and troubles on skin, liver, and kidney troubles can be occurred from mushroom intoxication. Recently the author reported as a collection of treatises regarding anti-tumor substances of mushroom (2005), antibiotic substances of mushroom (2006), living-body functional and control substance (2007), and medicinal mushroom commonly used in Korean herbal medicines (2008). In this sequel, published research data on protoplasm, nerve, stomach and intestines, malodor, and acute pain poison of mushroom are collected and reported.

• 한국식품영양과학회지
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• 제45권8호
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• pp.1083-1089
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• 2016

대부분의 여성은 폐경 이후 신체적 변화를 겪게 된다. 폐경의 증상으로는 홍조, 발한, 몸무게의 증가와 질 건조증 등이 있다. 본 연구에서는 여성의 폐경기 증상 완화에 효능이 있는 열 가지 종류의 생약을 조합하여 만든 혼합 추출물이 동물의 폐경 모델인 난소절제(ovariectomized, OVX) 레트에 어떠한 영향을 미치는지 알아보고자 하였다. 경구투여를 통해 각각 500 mg/kg/d, 1,000 mg/kg/d 농도의 열수 추출물(자음미단)을 8주간 섭취시켰으며, 양성대조군으로 에스트라디올($50{\mu}g/mL$)을 사용하였다. 실험을 통해 자음미단이 난소절제 레트에서 몸무게를 유의적으로 감소시켰으며, 간에서의 지방 함량 증가를 억제함을 확인하였다. 자음미단의 섭취는 페경 후 증가하는 혈액의 중성지방과 콜레스테롤 수치도 감소시켰다. 또한, 자음미단은 질의 무게를 증가시켰으며, 특히 질 내벽의 상피세포의 두께에 영향을 주었다. 자음미단의 1,000 mg/kg/d의 농도에서는 에스트로디올과 유사한 정도의 내벽 두께를 관찰할 수 있었다. 따라서 본 실험을 통해 자음미단이 폐경기 여성의 증상인 비만, 대사질환 및 질 건조증의 증상을 완화해줄 수 있으며, 이를 활용한 기능성 식품의 개발이 기대된다.

• 한국식품저장유통학회지
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• 제24권4호
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• pp.542-549
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• 2017

In this study, a peptide exhibiting antioxidant activity was isolated from sandfish (Arctoscopus japonicus) roe hydrolysate (SRH) in order to evaluate their practical uses as materials for manufacturing functional foods. The A. japonicus roe protein was hydrolyzed using Collupulin MG, and isolation of antioxidant peptide was performed using ultrafiltration (UF), prep-HPLC, and RP-HPLC. The SRH with a molecular weight below 3 kDa constituted about 38% of the whole hydrolysate, and the fraction with a molecular weight below 3 kDa showed significantly greater antioxidant activity compared to the original SRH and other fractions. The isolation fold of the antioxidant peptide isolated from SRH throughout the four-step procedure was 7.11-fold, and protein yield was 14.8%. The DPPH radical scavenging activity of isolated antioxidant peptide was above 90% at a concentration of 1.0 mg/mL, which was similar to that of the Trolox at a concentration of 0.1 mg/mL. These results suggested that the antioxidant peptide derived from A. japonicus roe could be a useful additive for producing functional foods and protein supplements. However, it is necessary to perform further study the structural characteristics of this antioxidant peptide isolated from A. japonicus roe.

• 한국식품영양학회지
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• 제33권6호
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• pp.645-654
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• 2020

Angelica gigas Nakai (A. gigas) easily changes its color during storage, and appropriate thermal treatment can improve storage stability through inactivation of enzymes such as polyphenol oxidase. Therefore, this study was performed to determine quality characteristics of dried A. gigas in response to high-temperature-short-time (HTST) treatment during storage. Dried A. gigas were treated at 120-180℃ for 10 min, the samples were stored at 4℃ and 50℃ for 10 weeks, and used for the analysis of qualities. Concerning the color values, the sample treated at 120℃ was similar to the control, and the color change was large when treated above 180℃. However, color difference (ΔE⁎ab) was lower in treated samples than in control. Browning index was similar for all the samples except for the sample treated at 180℃. Functional qualities (phenolics content, antioxidant activities, and level of major components) showed a slight difference according to storage periods in all samples without control, and nodakenin content was observed in control. The results of this study showed that HTST treatment improved storage stability such as stability of colors and browning index in dried A. gigas during storage, and the appropriate treatment temperature was 120℃ in terms of stability in color and browning index.