• 한국식품과학회지
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• 제49권1호
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• pp.56-62
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• 2017

The purpose of this study was to determine how seeding time changes the properties of soybean products meju and doenjang. Soybeans were seeded on the last day of May (5L), on the mid day of June (6M), and on the last day of June (6L), respectively. The 5L soybeans experienced a distinguishing hot and humid climate at the ripening stage, and these climate conditions resulted in smaller seed sizes. Fermentation briskly progressed in all doenjang until 120 days, and the 5L doenjang exhibited the lowest fermentation efficiency. The 5L soybeans showed the highest GABA, polyphenol contents, and ACE inhibitory activity, and the 6M soybeans showed the highest radical scavenging activity among the groups tested. In doenjang, the functional properties generally increased as the aging time approached the $120^{th}$ day, and the 6M doenjang showed the highest functional properties at the $120^{th}$ day. Therefore, 6M soybeans had the most appropriate characteristics when producing doenjang.

• 한국식생활문화학회지
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• 제22권5호
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• pp.633-644
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• 2007

The study results regarding the ingredient differences, sensory characteristics, purchasing type, usage and improvement direction for home-made traditional doenjang and factory produced commercial doenjang are as follows. The L-value indicates that home-made traditional doenjang has a higher value in average than the factory produce done, and the a-value indicates the opposite. Home-made traditional doenjang had higher water content than commercial doenjang ; however the pH values of commercial doenjang and home-made were 5.34 and 5.32 respectively, which was very similar. Factory produced commercial doenjang showed higher protein content than the home-made traditional doenjang. Regarding the correlation between ingredients, there was a significantly negative relationship between the L-value and a-value but a significantly positive relationship between the L-value and b-value. There were no significant relationship with water content, pH and protein content. For the color and taste, which are the sensory characteristics, commercial doenjang showed higher value than the traditional doenjang, but for smell, the values were similar. Regarding grittiness, the factory produced commercial doenjang had bigger particles than the traditional doenjang. Preference was a bit higher in the traditional doenjang. Of the 380 study subjects, most were from 40 to 49 years old (65.5%), and the most family type were nuclear families which was a total of 400 people (69%). Moreover, the most residential type was apartment which was 355people (61.2%), and for the monthly income, more than 2,510,000won was 48.3%. For the educational background, college education was 304 people (52.4%), and high school education was 199 people, 34.3%. In the usage, most of the people eat doenjang more than once a week, and usually their parents make the doenjang. People used both commercial doenjang and home-made traditional doenjang >home-made only >factory produced commercial doenjang only in that order. The reasons for using the home-made traditional doenjang aredelicate taste and flavor>more nutritious> anti-cancer ingredients in that order. The reason they use the factory produced commercial doenjang is because they don't know how to make it at home. The things that needed to be improved in the home-made traditional doenjang are bad smell> entire quality> flavor> color in order, indicating that studies for reducing bad smell are required. The things that needed to be improved in the factory produced commercial doenjang are taste & flavor> entire quality>bad smell> color in that order, indicating that people are more concern about it tasting like home-made than the smell. From the above results, we can see that better functional doenjang should be developed for family health and to increase the consumption of the doenjang, which has good functional psychological activities, also more various types of foods that use doenjang and scientific studies to reduce the home-made doenjang smell should be continuously studied. Moreover, studies on how to make the factory produced commercial doenjang taste more like traditional doenjang should be performed.

• KSBB Journal
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• 제27권6호
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• pp.361-366
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• 2012

We investigated the anti-diabetic activity and enzymatic activity of 24 commercial doenjang samples certified for traditional foods. Twenty four doenjang samples showed the wide ranges in enzymatic activities (protease activities 0-50.45 unit/g, ${\alpha}$-amylase activities 0-675.9 unit/g, ${\beta}$-amylase 13.6-308.6 unit/g), and there were no difference in enzymatic activity by the producing region. To evaluate the potential anti-diabetic activity of 24 doenjang samples, we examined the effect of doenjang methanol extract (DME) on 2-[n-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amyno]-2-deoxy-d-glucose (2-NBDG) uptake. Ten samples among 24 samples significantly stimulated the uptake of 2-NBDG. When the cells were treated with DME at 400 ug/mL, No. 17 and 23 specially stimulated 2-NBDG uptake by 1.23-fold and 1.25-fold, respectively, compared with untreated control cell. And there were no cytotoxicity in the C2C12 cells treated with DME at concentration of 500 ug/mL. Among 24 samples, No. 6, 7, 12, 21 and 24 showed the ${\alpha}$-glucosidase inhibitor activity at concentration of 10 mg/mL; however, they were less effective than acarbose which is a commercial ${\alpha}$-glucosidase inhibitor.

• Nutrition Research and Practice
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• 제6권6호
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• pp.520-526
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• 2012

Various forms of fermented soybean products are well documented for their health benefits. The efficacy of anti-obesogenic effect of Doenjang, one of the most commonly used seasonings in Korean cuisine, has been reported only in animal models; thus, an evaluation of Doenjang needs to be conducted in human studies. We aimed to test the hypothesis that Doenjang supplementation reduces body weight and changes body composition in overweight adults. A total of 51 overweight adults participated in this study. A group of males with BMI ${\geq}23kg/m^2$ and waist to hip ratio (WHR) ${\geq}$ 0.90, and a group of females with BMI ${\geq}23kg/m^2$ and WHR ${\geq}$ 0.85 were randomly assigned to either a Doenjang supplement (9.9 g dry/day) group or a placebo group for a 12-week randomized, double-blind and placebo-controlled study. Anthropometric parameters, abdominal fat distribution by computerized tomography (CT) and blood components were measured before and after the intervention period. After the 12-week study, the Doenjang supplementation group had significant reductions in body weight (kg), body fat mass (kg) and body fat (%) compared to the placebo group, the supplementation of Doenjang resulted in a significant reduction in visceral fat ($cm^2$), although no changes were observed in total and subcutaneous fat are as ($cm^2$), serum lipid profiles and dietary intakes. The present study demonstrated that daily supplementation of 9.9 g dry/day of Doenjang for 12 weeks reduces body weight and visceral fat in overweight adults.

• Food Science and Biotechnology
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• 제18권4호
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• pp.1030-1034
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• 2009

A fermenting microorganism involved in formation of ortho-dihydroxyisoflavones (ODIs) during aging of doenjang (Korean fermented soybean paste) has been investigated. Microorganisms in ODI-containing doenjang were isolated by cultivating on yeast mold (YM) agar medium containing 0-7% NaCl. ODI formation of the isolated strains was examined by gas chromatography/mass spectrometry (GC/MS) analysis after cultivation in modified YM broth or soybean extract medium. An ODI-producing microbe was identified as Bacillus subtilis HS-1 based on 16S rRNA gene sequence analysis. The strain has produced 8-hydroxydaidzein as a major product during growth in the modified YM broth or soybean extract medium. Therefore, it was concluded that one of the microorganisms involved in the formation of ODIs in doenjang was B. subtilis HS-1.

• 생명과학회지
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• 제13권5호
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• pp.559-568
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• 2003

본 연구는 된장 숙성 중 각종 미생물중에서 항산화 및 혈전 용해효소를 분비하는 균주를 분리 동정하고 이들 균주를 이용하여 기능성 항산화 및 혈전용해활성이 있는 된장을 제조하였다. 본 실험에서 된장에서 분리한 균주 중 1차적으로 항산화 활성 및 혈전용해 활성이 우수한 균주 BH-23을 선별하였다. 분리, 선별한 균주 BH-23은 형태학적, 생리화학적 및 생화학적 특성을 확인한 결과 Bacillus subtilis으로 잠정 적으로 결정하였고, 편의상 Bacillus subtilis BH-23으로 명명하였다. Bacillus subtilis BH-23의 최적 생육조건은 온도 $40^{\circ}C$, pH 5.0에서 가장 좋았고, 식염이 15%에서도 성장속도는 좋았다. 그리고 Bacillus subtilis BH-23의 항산화 활성 및 항혈전효소의 최적생성 조건은 본 균주의 성장조건과 거의 일치하였다. Bacillus subtilis BH-23을 이용하여 만든 제품과 시판된장과 비교 분석한 결과 Bacillus subtilis BH-23로 만든 된장은 시판된장보다 암모니아 취가 거의 없었으며 된장의 색깔도 탁하지 않았으며 풍미도 우수하였다. 따라서 Bacillus subtilis BH-23으로 만든 된장을 본래의 제품에 큰 영향이 없으면서 항산화 및 혈전용해 효소을 분비하는 기능성 된장을 제조할 수 있을 것으로 생각되어진다.

• 한국식품저장유통학회지
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• 제17권2호
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• pp.243-249
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• 2010

본 연구는 세이지의 다양한 생리적 기능성과 우리 전통된장을 접목하여 허브를 첨가한 된장을 개발하고자 된장의 염도와 세이지분말 첨가량을 달리한 후 반응표면분석법으로 최적조건을 얻고자 관능특성을 조사하였다. 그 결과, 색은 1.20~2.70, 냄새는 1.60~3.20, 맛은 1.40~3.50, 조직감은 1.60~3.50, 전반적인 기호도는 1.60~3.05의 값을 나타내어 실험구의 조건에 따라 차이를 보였다. 이차회귀식에 의하여 형성된 반응표면 분석 결과 색, 냄새, 맛, 조직감 및 전반적인 기호도의 $R^{2}$가 0.11~0.41로 낮은 편이었으며, 5% 이내에서 유의성이 인정되지 않았다. 관능검사에서 가장 높게 평가된 것은 색은 염도 21.50%, 세이지분말 첨가량 3.10%일 때 2.91, 냄새는 염도 22.52%, 세이지분말 첨가량 3.68%일 때 3.21로, 맛은 염도 8.62%, 세이지분말 첨가량 4.46%일 때 2.87으로, 조직감은 염도 8.00%, 세이지분말 첨가량 6.06%일 때 2.88로, 전반적인 선호도는 염도 20.40%, 세이지분말 첨가량 2.66%를 첨가하였을 때 2.74로 나타났다. 한국인에게 익숙하지 않은 허브이지만, 세이지를 첨가한 된장이 일반된장 보다는 높은 점수로 평가되어 기능성 된장으로의 가능성을 확인할 수 있었다.

• 한국식품영양학회지
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• 제29권5호
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• pp.785-794
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• 2016

This research was conducted to evaluate quality changes in traditional Doenjang and manufactured Doenjang during a storage period of 8 weeks. Low-salt Doenjang and commercial Doenjang were purchased from different manufacturers and proximate analysis as well as changes in isoflavone, polyphenol, flavonoid contents of the samples were investigated using a mass spectrophotometer. The salinity of traditional Doenjang, low salt Doenjang, and commercial Doenjang were $13.2{\pm}1.15$, $7.17{\pm}2.74$, $10.67{\pm}0.35%$, respectively and the salt concentrations of the soybean pastes did not change during storage. After 8 weeks at $35^{\circ}C$, chromatic values of all the paste samples decreased somewhat, with traditional Doenjang exhibiting fewer changes as compared to manufactured Doenjang. Amino acid nitrogen, acidity, microbial population all tended to increase with time, although some samples showed fluctuations during the test period. Moreover, the total isoflavone contents of traditional Doenjang increased with storage time while that of manufactured Doenjang tended to decrease. The isoflavone aglycone was shown to be the highest in traditional Doenjang, while isoflavone glycoside was abundant in manufactured Doenjang. Total flavonoid contents showed similar trends regardless of samples; initial contents of total flavonoid was 0.6 mg/g which increased to more than twice to 1.4 mg/g at the end of storage period. Composition profile of Doenjang extracts was analyzed using UPLC-Q-ToF.

• 한국식품영양과학회지
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• 제41권12호
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• pp.1842-1846
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• 2012

된장과 고추장에 대하여 Bacillus cereus 포자를 $10^5$ spore/g 접종하고 이의 저감화에 적용 가능한 허들로서 additive(95% ethanol 3%, 1% oregano 추출물 0.3%), Joule 가열($95^{\circ}C$, 5분) 및 초고압처리(500 MPa, $45^{\circ}C$, 5분)를 적용하여 Bacillus cereus 포자의 살균효과와 저장성을 분석하였다. 된장에 있어 AJ(additive+Joule 가열) 및 AJP(additive+Joule 가열+초고압) 처리구는 각각 2.80 log 및 3.74 log 감소효과를 보였고 고추장의 경우에는 각각 4.71 log 및 5.60 log 감소를 보여 Joule 가열처리구는 된장과 고추장에 있어서 additive 처리구와 함께 Bacillus cereus 포자의 살균에 상당한 시너지 효과를 나타내는 것으로 판단되었다. 한편 $30^{\circ}C$ 저장시험에서 additive, Joule 가열 및 초고압 병행 처리는 된장 및 고추장에서 낮은 수준의 포자를 유지하는 데에도 유효한 효과를 보여 AJ(additive+Joule 가열) 및 AJP(additive+Joule 가열+초고압) 처리기법은 된장 및 고추장의 Bacillus cereus 포자에 대한 효율적인 살균방법으로서 적용이 가능할 것으로 보였다.

• 한국식품영양과학회지
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• 제32권7호
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• pp.1002-1010
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• 2003

생리활성이 우수하다고 알려진 된장의 소비확대를 위하여 시판 된장을 이스트 발효 식빵 제조에 이용하고자 식빵 배합비에 7종(A; 재래식 된장, B∼E; 시판 개량식 된장, F;시판 Miso type 된장, G; Japanese Miso)의 동결건조한 된장분말을 2.5∼10.0% 첨가하여 발효력과 오븐팽창력을 관찰하고 빵품질을 물리적 측면에서 조사하였다. 사용된 시판된장 7종의 이화학적 특성치로는 일반성분을 비롯하여 아미노태질소, 총산도, 환원당, 유리당, 염도, 유기산 함량 및 단백질분산지수(PDI)를 제시하였다. 빵 배합비에 된장분말 5.0% 이하의 첨가는 오븐팽창력의 현저한 증가로 모든 된장 첨가식빵 부피가 증가하여 무첨가구에 비하여 최대 13%까지 높게 나타났다. 또한 된장첨가로 기계적 조직감 측정치인 경도(hardness), 검성 (gumminess), 씹힘성(chewiness) 등이 감소하여 전반적으로 부드러워졌고, 빵의 색은 어두워졌다. 그러므로 식빵제조에 시판된장의 사용은 빵의 물리적 품질 향상에 매우 긍정적인 효과를 가져왔다. 이때 식빵의 부피나 조직감은 된장 종류나 첨가수준에 의하여 독립적으로 영향을 받을 뿐만 아니라 이들 처리조합의 상호작용에 의하여도 크게 영향을 받는 것으로 나타났다.