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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Antidiabetic Activity and Enzymatic Activity of Commercial Doenjang Certified for Traditional Foods

전통식품 품질인증 일부 시판 된장의 효소활성 및 항당뇨 활성

  • Lee, So-Young (Fermentation and Functional Research Group, Korea Food Research Institute) ;
  • Kim, In-Sun (Fermentation and Functional Research Group, Korea Food Research Institute) ;
  • Park, So-Lim (Fermentation and Functional Research Group, Korea Food Research Institute) ;
  • Lim, Seong-Il (Fermentation and Functional Research Group, Korea Food Research Institute) ;
  • Choi, Hye-Sun (Department of Agro-food resources, National Academy of Agricultural science, RDA) ;
  • Choi, Shin-Yang (Fermentation and Functional Research Group, Korea Food Research Institute)
  • 이소영 (한국식품연구원 발효기능연구단) ;
  • 김인선 (한국식품연구원 발효기능연구단) ;
  • 박소림 (한국식품연구원 발효기능연구단) ;
  • 임성일 (한국식품연구원 발효기능연구단) ;
  • 최혜선 (농진청 국립농업과학원 농식품자원부) ;
  • 최신양 (한국식품연구원 발효기능연구단)
  • Received : 2012.11.15
  • Accepted : 2012.11.20
  • Published : 2012.12.31
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Abstract

We investigated the anti-diabetic activity and enzymatic activity of 24 commercial doenjang samples certified for traditional foods. Twenty four doenjang samples showed the wide ranges in enzymatic activities (protease activities 0-50.45 unit/g, ${\alpha}$-amylase activities 0-675.9 unit/g, ${\beta}$-amylase 13.6-308.6 unit/g), and there were no difference in enzymatic activity by the producing region. To evaluate the potential anti-diabetic activity of 24 doenjang samples, we examined the effect of doenjang methanol extract (DME) on 2-[n-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amyno]-2-deoxy-d-glucose (2-NBDG) uptake. Ten samples among 24 samples significantly stimulated the uptake of 2-NBDG. When the cells were treated with DME at 400 ug/mL, No. 17 and 23 specially stimulated 2-NBDG uptake by 1.23-fold and 1.25-fold, respectively, compared with untreated control cell. And there were no cytotoxicity in the C2C12 cells treated with DME at concentration of 500 ug/mL. Among 24 samples, No. 6, 7, 12, 21 and 24 showed the ${\alpha}$-glucosidase inhibitor activity at concentration of 10 mg/mL; however, they were less effective than acarbose which is a commercial ${\alpha}$-glucosidase inhibitor.

Keywords

References

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