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Development of SSLP Marker Targeted to P34 Null Gene in Soybean (콩 P34 단백질 결핍 유전자를 이용한 SSLP 마커 개발)

  • Yang, Kiwoung;Ko, Jong-Min;Lee, Young-Hoon;Jeon, Myeong Gi;Jung, Chan-Sik;Baek, In-Youl;Kim, Hyun-Tae;Park, Keum-Yong
    • Korean Journal of Breeding Science
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    • v.42 no.5
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    • pp.502-506
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    • 2010
  • Soybean seed possesses about 15 allergenic proteins recognized by IgEs from soy-sensitive human. The allergenic impact of soybean proteins limit its extensive usage in a broad range of processed foods. Soybean protein P34 or Gly m Bd 30k of the cysteine protease family is one of the major allergen of the soybean seed. P34-null soybean, PI567476, was identified among soybean (Glycine max & Glycine soja Sieb. and Zucc) of approximately 16,226 accessions from USDA soybean germplasm screened. Also, for P34 gene (Williams 82; whole genome sequence cultivar) and P34 null gene (PI567476) comparative analysis of sequences listed in the NCBI database showed the presence of a SSLP (Simple Sequence Length Polymorphism) of 4 base pair. So, a SSLP marker was designed to reveal the polymorphism of the locus. In this study, a population of 339 $F_2$ recombinant inbred lines generated by cross between Taekwang (Glycine max) and PI567476 was used to select $F_{2:3}$ plant of a P34 null gene. The result separation rate Taekwang type, heterozygous type and PI567476 type were shown in 85: 187: 67 since single gene is concerned in as the separation rate of 1:2:1 in $X^2{_{0.05}}=5.99$, df=2. In future, selected plant will identify protein level, whether P34 null protein is equal to P34 null gene.

Construction of an RNase P Ribozyme Library System for Functional Genomics Applications

  • Hong, Sun-Woo;Choi, Hyo-Jei;Lee, Young-Hoon;Lee, Dong-Ki
    • Genomics & Informatics
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    • v.5 no.1
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    • pp.6-9
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    • 2007
  • An RNase P ribozyme library has been developed as a tool for functional genomics studies. Each clone of this library contains a random 18-mer and the sequence of M1 RNA, the catalytic subunit of RNase P. Repression of target gene expression is thus achieved by the complementary binding of mRNA to the random guide sequence and the successive target cleavage via M1 RNA. Cellular expression of the ribozyme expression was confirmed, and EGFP mRNA was used as a model to demonstrate that the RNase P ribozyme expression system can inhibit the target gene expression. The constructed RNase P ribozyme library has a complexity of $1.4\times10^7$. This novel library system should become a useful in functional genomics, to identify novel gene functions in mammalian cells.

The Correlations between the Balance Test, functional movement, Visual Perception Test and Functional Independent Measure in Stroke Patients (뇌졸중 환자의 균형, 기능적 보행, 시지각, 일상생활 평가도구의 상관성)

  • Lee, Dong-Jin;Kim, Seong-Yeol;Song, Chang-Ho
    • The Journal of Korean Physical Therapy
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    • v.21 no.2
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    • pp.39-45
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    • 2009
  • Purpose: The purpose of this study was to determine correlations between the Berg Balance Scale (BBS), Functional Reach Test (FRT), Timed Up & Go (TUG), Motor-Free Visual Perception Reach Test Vertical format (MVPT-V), Functional Independence Measure (FIM). Methods: The subjects used in this study were 35 stroke patients from Cheongju ST. Mary's hospital. Balance was measured by BBS, FRT. Functional mobility was measured by TUG. Visual perception was measured by MVPT-V. FIM was used to evaluate the activities of daily living. Data was analyzed using pearson product correlation. The TUG and MVPT-V index were analyzed by linear regression. Results: There was a statistically significant difference between FRT and BBS (r=0.89, p<0.01), FIM (r=0.74, p<0.05), MVPT-V (r=0.40, p<0.05), and TUG (r=-0.36, p<0.05). There was significant statistical differences between TUG and MVPT-V (r=-0.64, p<.01). However, statistically significant differences were observed between BBS and FIM (r=0.79, p<0.01). The visual close item of the MVPT-V showed the strongest variance in predicting TUG. Conclusion: The use of both quantitative and qualitive scales was shown to be a good measuring instrument for the classification of general clinical performances of stroke patients. In particular, the results suggest that the visual perception test may be able to predict functional locomotion in stroke patients.

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Evaluation of Functional Ability and Nutritional Risk according to Self-Rated Health (SRH) of the Elderly in Seoul and Kyunggi-do (서울 및 경기지역 노인의 건강자가평가에 따른 기능적 건강 및 영양위험 평가)

  • Choi, Yoon-Jung;Park, Yu-Sin;Kim, Chan;Jang, Yu-Kyung
    • Journal of Nutrition and Health
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    • v.37 no.3
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    • pp.223-235
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    • 2004
  • A functional ability and adequate nutritional status are the major determinants of health status, Self-rated health (SRH) is a worldwide method to assess health status and it is recognized as a predictor of morbidity and mortality in the elderly, This study was designed to evaluate the functional ability and nutritional risk according to SRH in the elderly. Four hundred nine free-living elderly people (118 male, 291 female), aged $\geq$ 65 years were interviewed by trained interviewers using structured questionnaires including demographic information, SRH, anthropometric measurements, functional ability, general health status, and nutritional risk. SRH was divided into three status such as “Good”, “Moderate” and “Poor” status. And all the data were analyzed by oneway ANOVA, spearman correlation, and x$^2$ analysis using SPSS 9.0 version at p 〈 0.05. Of all the subjects, 48.9% perceived their health status as “poor”, and their functional abilities (activities of daily living, instrumental activities of daily living) were more impaired than their counterparts (“good” and “moderate”). Poor self-rated health was also related to: a higher prevalence of illnesses (p 〈 0,001) especially in hypertension, arthritis. Self-rated health was significantly related to food security (p 〈 0.001), food enjoyment (p 〈 0.001) ,and nutritional knowledge (p = 0.0 13). Also NSI checklist total score was the highest in “poor” health status (p 〈 0.001). Better self-rated health was related to better food security, and better food enjoyment. However, smoking, alcoholic intake, exercise, eating behaviors, and demographic characteristics were not significantly different among the three SRH status. SRH was closely related to chronic diseases, functional ability, and nutritional risk in the elderly. Therefore, public health strategies for the elderly should be focused on the elderly who are “poor” in SRH, to improve nutritional status and functional ability, and to reduce risk factors of chronic diseases.

The Effects of Driving Rehabilitation Functional Training on Visual Perception and Driving Reaction Velocity (운전시뮬레이터 훈련이 시 지각 및 운전 반응 속도에 미치는 효과)

  • Lee, Jungsook;Kim, Sungwon
    • Journal of The Korean Society of Integrative Medicine
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    • v.5 no.4
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    • pp.77-81
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    • 2017
  • Purpose : This study examined the effects of driving rehabilitation functional training on visual perception ability and driving reaction velocity. Those subjects were put under MVPT-3 test to see their visual perceptual functions before and after the 4weeks' driving rehabilitation function training and then put to TMT A-type test to see their driving reaction velocity performance. The followings are the results of this study. Methods : Using a driving simulator, driving rehabilitation functional training was performed targeting men and women aged in 20s 20 minutes per time, two times per week, for a month. Results : As for the change in visual perception, the Raw Score of MVPT-3 very significantly increased (p<.01), and the Standard Score also very significantly increased (p<.01). As for the change in reaction velocity, TMT A-type very significantly decreased (p<.01), and TMT B-type also very significantly decreased (p<.01). Conclusion : It could be found that driving rehabilitation functional training should be effective for both visual perception and reaction velocity. Consequently, the driving rehabilitation function training can be applied to clinics as training method for functional recovery and improvement of visual perceptual functions and driving reaction velocity performance ability of the patients. Thus, various functional programs should be studied in the future.

The Assessment of the Postural Control Ability of the Volleyball Players With Functional Ankle Instability Using Balance Master System

  • Kim, Ho-Sung;Ahn, Chang-Sik;Choi, Jong-Duk
    • Physical Therapy Korea
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    • v.15 no.4
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    • pp.18-26
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    • 2008
  • The present study was aimed at investigating the postural control ability of volleyball players with functional ankle instability. The subjects were 26 male volleyball players were divided into 2 groups (13 subjects with functional ankle instability and 13 subjects with ankle stability) who could evaluate Questionnaire. All the male participants were tested by a Balance Master System. This study were to measure of static balance ability, dynamic balance ability, motor function the difference between functional ankle instability group and control group. Ankle instability group and stable group in postural sway ($^{\circ}/sec$) on film surface with eye closed in modified clinical test sensory interaction on balance, and left unilateral stance with eye opened and closed were significantly different (p<.05). The ankle instability group and stable group in limit of stability were significantly different (p<.05). The ankle instability group and stable group in left/right rhythmic weight shirt were significantly different (p<.05). The ankle instability group and stable group in turn time (sec) & turn sway ($^{\circ}$) during step/quick turn and end sway ($^{\circ}/sec$) in tandem walk were significantly different (p<.05). This study showed that volleyball players with functional ankle in stability were effected postural control ability by static balance & dynamic balance ability. Further study is needed to measure various athletic with functional ankle instability for clinical application.

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The Effects of Functional Electrical Stimulation on Forced Vital Capacity and Phonation Capabilities in Children with Spastic Cerebral Palsy

  • Ju, Joung-Youl;Kang, Kwon-Young;Shin, Hee-Joon
    • Journal of International Academy of Physical Therapy Research
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    • v.2 no.2
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    • pp.339-343
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    • 2011
  • The purpose of this study is to see the effect of functional electrical stimulation on forced vital capacity and alternating motion rate in children with spastic cerebral palsy. This study divided 20 children with spastic cerebral palsy into two groups; functional electrical stimulation treatment group and control group. Functional electrical stimulation treatment group had 20min per day treatment three times a week for four weeks and the control group did not have any treatment. Before and after intervention, this study measured forced vital capacity and alternate motion rate(/peo/,/teo/) for all children. Forced vital capacity showed statistically significant increase for the group with functional electrical stimulation(p<.05) while the control group did not show any significant increase(p>.05). Alternate motion rate showed statistically significant increase for the group with functional electrical stimulation(p<.05) while the control group did not show any significant increase(p>.05). This result shows that functional electrical stimulation affected the ability of the children with spastic cerebral palsy who have decreased breathing and phonation capability.

The Study of Muscle Activity on Functional Reaching (기능적 팔 뻗기 시 근 활성에 관한 연구)

  • Chae, Jung-Byung
    • PNF and Movement
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    • v.11 no.1
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    • pp.55-62
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    • 2013
  • Purpose : This study was assessed muscle activity and onset time in trunk and upper extremity on functional reaching. Methods : The participant was 18 female(young 10, old 8). As functional reaching, we collection data by using EMG(MP150) on transverse abdominis, external oblique, erector spinae, deltoid middle and serratus anterior. Results : 1) In functional reaching, transverse abdominis, external oblique, erector spinae and deltoid middle muscle activity was augmented on old female(p>.05). Serratus anterior was augmented on young female(p>.05). 2) In functional reaching, transverse abdominis and erector spinae muscle onset time is significantly faster old female than young female(p<.05). External oblique and serratus anterior muscle onset time is faster old female than young female(p>.05). 3) As increase of age muscle activity of external oblique was more increased that we found .511 a coefficient correlation and onset time more faster on transverse abdominis and erector spinae were each -.492 and -.554 coefficient correlation. Conclusion : The muscle activity and onset time was difference in functional reaching according to ageing and task context. It is necessary concentration and attention to old female than young female. Therefore, these results suggest that importance of anticipatory postural control and selective strategy of postural control.

GENERALIZED STABILITIES OF CAUCHY'S GAMMA-BETA FUNCTIONAL EQUATION

  • Lee, Eun-Hwi;Han, Soon-Yi
    • Honam Mathematical Journal
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    • v.30 no.3
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    • pp.567-579
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    • 2008
  • We obtain generalized super stability of Cauchy's gamma-beta functional equation B(x, y) f(x + y) = f(x)f(y), where B(x, y) is the beta function and also generalize the stability in the sense of R. Ger of this equation in the following setting: ${\mid}{\frac{B(x,y)f(x+y)}{f(x)f(y)}}-1{\mid}$ < H(x,y), where H(x,y) is a homogeneous function of dgree p(0 ${\leq}$ p < 1).