• Reproductive and Developmental Biology
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• v.36 no.3
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• pp.193-198
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• 2012

This study was to examine expression of the recombinant full-length adiponectin (recombinant adiponectin) in insect ovarian cell culture system and to characterize structural properties of the recombinant adiponectin secreted in medium. Gene construct encoding the recombinant adiponectin contained N-terminal collagen-like domain (110 Amino Acids, AAs), C-terminal globular domain (137 AAs) and C-terminal peptides for detection with V5 antibody (26 AAs included adaptor peptide) and purification using the 6xHis tag (6 AAs). The approximate molecular weight of the product (monomer) was 35 kDa. Molecular mass species of the expressed recombinant adiponectin were monomer (~35 kDa), dimer (~70 kDa), trimer (~105 kDa) and hexamer (~210 kDa). The major secreted species were the LMW forms, such as monomer, dimer, and trimer. There was MMW of hexamer as minor form. HMW multimers (~300 kDa) were shown as a tracer or not detected on the SDS-PAGE in several experiments (data not shown). The multimer forms in this study were not compatible to those in animal or human serum and adipose tissue by other researcher's study in which the major multimer forms were HMW. By protein denaturing experiments with reducing reagent (${\beta}$-MeOH), anionic detergent (SDS) and heat ($95^{\circ}C$) on the SDS-PAGE, not all adiponectin multimers seemed to have disulfide bond linked structure to form multimers. The recombinant adiponectin which expressed in insect ovarian cell culture system seemed to have the limitation as full physiological regulator for the application to animal and human study.

• Journal of the Korea Concrete Institute
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• v.15 no.1
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• pp.102-109
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• 2003

The grouted splice steeve has been applied widely due to its superior construction efficiency, such as the unnecessity of post concrete and the large allowable limit to the arrangement of reinforcing bars. However, studies on grout-filled splice steeve still have not been sufficiently peformed. The purpose of this study is to investigate the confining effect of mortar grouted splice sleeve on reinforcing bar, known to strengthen the bond capacity between grout mortar and reinforcing bar. To accomplish this objective, totally 6 full-sized specimens were made and tested under monotonic loading. Each specimens were equipped with strain gauges at the 12 location of sleeve and reinforcing bar. The experimental variables adopted in this study are embedment length and size of reinforcing bars. Following conclusions are obtained; 1) Under ultimate strength condition, the confining pressure of grouted splice sleeve calculated from measured tangential and axial strain of the sleeve is over $200{\sim}300kgf/{cm}^2$ at any location of sleeve and improved with reduction in embedment length of reinforcing bar. 2) Untrauer and Henry's equation which describe bond strength of mortar as a function of its compressive strength and confining pressure, predicted the measured bond capacity of this test within the 5% limits.

• Journal of the Korean Geotechnical Society
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• v.24 no.10
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• pp.147-160
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• 2008

This paper presents the results of full-scale loading tests performed on 54 passive anchors and 4 group anchored footings grouted to various lengths at several sites in Korea. The test results, the failure mechanisms as well as uplift capacities of rock anchors depend mostly on rock type and quality, embedded fixed length, properties of the discontinuities, and the strength of rebar. Anchors in poor quality rocks generally fail along the grout/rock interfaces when their depths are very shallow (a fixed length of less than 1 m). However, even in such poor rocks, we can induce a more favorable mode of rock pull-up failure by increasing the fixed length of the anchors. On the other hand, anchors in good quality rocks show rock pull-up failures with high uplift resistance even when they are embedded at a shallow depth. Laboratory test results revealed that a form of progressive failure usually occurs starting near the upper surface of the grout, and then progresses downward. The ultimate tendon-grout bond strength was measured from $18{\sim}25%$ of unconfined compressive strength of grout. One of the important findings from these tests is that the measured strains along the corrosion protection sheath were so small that practically the reduction of bond strength by the presence of sheath would be negligible. Based on test results, the main parameters governing the uplift capacity of the rock anchor system were determined. By evaluation of the ultimate uplift capacity of anchor foundations in a wide range of in situ rock masses, rock classification suitable for a transmission tower foundation was developed. Finally, a very simple and economical design procedure is proposed for rock anchor foundations subjected to uplift tensile loads.

• Steel and Composite Structures
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• v.4 no.5
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• pp.333-353
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• 2004

Strength of reinforced concrete beams can easily be increased by the use of externally bonded CFRP composites. However, the mode of failure of CFRP strengthened beam is usually brittle due to tension-shear failure in the concrete substrate or bond failure near the CFRP-Concrete interface. In order to improve the ductility of CFRP strengthened concrete beams, critical variables need to be investigated. This experimental and analytical research focused on a series of reinforced concrete beams strengthened with CFRP composites to enhance the flexural capacity and ductility. The main variables were the amount of CFRP composites, the amount of longitudinal and shear reinforcement, and the effect of CFRP end diagonal anchorage system. Sixteen full-scale beams were investigated. A new design guideline was proposed according to the effects of the above-mentioned variables. The experimental and analytical results were found to be in good agreement.

• Proceedings of the Korea Concrete Institute Conference
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• 2001.05a
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• pp.63-68
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• 2001

It is now common practice to strengthen reinforced concrete beams by steel plates to their tention surfaces. Such plated beams are designed for flexure using conventional prediction equation and assumption that full bond will be developed between the concrete and the plates. Very advanced design rules have already been developed at the University of Adelaide for adhesive bonding steel plates to reinforced concrete beams in order to prevent premature debonding. This work on plated continuous reinforced concrete beams is to determine experimentally whether these design rules, that were developed for steel plated simply supported beams, could be applied to steel and FRP plated continuous beams. This paper also suggests how to increase the ductility of plated beams.

• Journal of Institute of Control, Robotics and Systems
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• v.7 no.5
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• pp.399-406
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• 2001

This paper presents a dynamic modeling and a sliding mode controller for the high-speed/high-accuracy position control system. The selected target system is the wire bonder assembly which is used in the semiconductor assembly process. This system is a reciprocating one around the pivot point that consists of VCM(voice coil motor) as an actuator and transducer horn as a bonding tool. For the modeling elements, the sys-tem is divided into electrical circuit, magnetic circuit and mechanical system. Each system is modeled using the bond graph method and united into the full system. Two major aims are considered in the design of the controller. The first one is that the horn must track the given reference trajectory. The second one is that the controller must be realizable by using the DSP board. Computer simulation and experimental results show that the designed sliding mode controller provides better performance than the PID controller.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2000.10a
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• pp.83-89
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• 2000

This paper presents a dynamic modeling and a sliding mode controller for the high-speed / high-accuracy position control system. Selected target system is the wire bonder head assembly which is used in semiconductor assembly process. This system is a reciprocating one around the pivot point that consists of VCM(voice coil motor) as a actuator and transducer horn as a bonding tool. For the modeling elements, the system is divided into electrical circuit, magnetic circuit and mechanical system. Each system is modeled by using the bond graph method and united into the full system. Two major aims are considered in the design of the controller. The first one is that the horn must track the given reference trajectory. The second one is that the controller must be realizable by using the DSP board. Computer simulation and experimental results show that the designed sliding mode controller provides better performance than the PID controller.

• Journal of the Korean Ceramic Society
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• v.12 no.3
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• pp.8-12
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• 1975

The crystal structure of synthetic magnesite has been studied by X-ray method. Magnesite is trigonal R3c, with a=4.637$\AA$, c=15.023$\AA$ and Z=6. Intensity data were collected with a Rigaku automated four-circle diffractometer and Mo-K$\alpha$ radiation. The structure was refined by the full-matrix least squares method using anisotropic thermal parameters. The final R index for 234 reflections is 0.037. The C-O and Mg-O bond lengths were 1.283 and 2.105$\AA$, respectively. The interatomic angles of three kinds of O-Mg-O were 88.25, 91.75 and 180.00$^{\circ}$, respectively. It is clarified that the distortion of the Mg-O6 octahedron in magnesite is smaller than that of Ca-O6 in calcite.

• Bulletin of the Korean Chemical Society
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• v.29 no.5
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• pp.963-968
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• 2008

For the [$Cr_2(H_2tmp)_2Cl_4$] compound, simplified models with two bridging methoxo ligands have been studied. The influence of the bridging Cr-O-Cr bond angles on the exchange coupling between metal atoms in the model compound has been analyzed by means of density functional calculations with the broken-symmetry approach. Coupling constant calculated for the full structure is in good agreement with the experimentally reported value, confirming the validity of the computational strategy used in this work to predict the exchange coupling in a family of related dinuclear Cr(III) compounds. The calculations indicate a good correlation between the calculated coupling constant and the sum of the squared energy gap of three pairs of metal $t_{2g}$ OMSOs with a limited variation of the Cr-O-Cr angle. The spin density distribution and the mechanism of magnetic coupling interactions are discussed.

• Archives of Pharmacal Research
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• v.19 no.1
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• pp.71-73
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• 1996

The molecular structure of pirprofen, 3-chloro-4-(2,5-dihydro-1H-pyrrol-1-yl)-.alpha.-methyl-benzeneacetic acid, was determined by single crystal X-ray diffraction analysis. The compound was recrystallized from a mixture of chloroform and toluene in triclinic, space group P over $\bar1,\; with\; a=4.577(1),\; b=11.213(2),\; C=12.485(2){\AA},\alpa.=107.39(1),\;\beta=97.79(1),\;\gamma=92.03(2),\; and Z=2$ The calculated density is $1.384 g/cm^3$. The structure was solved by the direct method and refined by full matrix least-squares procedure to the final R value of 0.034 for 1681 independent reflections. The non-aromatic dihydropyrrol group is found to be coplanar to the central aromatic ring. The molecules are dimerized through the intermolecular hydrogen bonds at the carboxyl group in the crystal.