• Reproductive and Developmental Biology
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• 제36권3호
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• pp.193-198
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• 2012

This study was to examine expression of the recombinant full-length adiponectin (recombinant adiponectin) in insect ovarian cell culture system and to characterize structural properties of the recombinant adiponectin secreted in medium. Gene construct encoding the recombinant adiponectin contained N-terminal collagen-like domain (110 Amino Acids, AAs), C-terminal globular domain (137 AAs) and C-terminal peptides for detection with V5 antibody (26 AAs included adaptor peptide) and purification using the 6xHis tag (6 AAs). The approximate molecular weight of the product (monomer) was 35 kDa. Molecular mass species of the expressed recombinant adiponectin were monomer (~35 kDa), dimer (~70 kDa), trimer (~105 kDa) and hexamer (~210 kDa). The major secreted species were the LMW forms, such as monomer, dimer, and trimer. There was MMW of hexamer as minor form. HMW multimers (~300 kDa) were shown as a tracer or not detected on the SDS-PAGE in several experiments (data not shown). The multimer forms in this study were not compatible to those in animal or human serum and adipose tissue by other researcher's study in which the major multimer forms were HMW. By protein denaturing experiments with reducing reagent (${\beta}$-MeOH), anionic detergent (SDS) and heat ($95^{\circ}C$) on the SDS-PAGE, not all adiponectin multimers seemed to have disulfide bond linked structure to form multimers. The recombinant adiponectin which expressed in insect ovarian cell culture system seemed to have the limitation as full physiological regulator for the application to animal and human study.

• 콘크리트학회논문집
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• 제15권1호
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• pp.102-109
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• 2003

모르타르 충전식 철근이음은 철근위치의 오차를 쉽게 흡수하여 시공이 용이하고 또한 콘크리트 타설을 최소화할 수 있는 프리캐스트 공법의 철근이음 방법중 하나로서 적용하는 경우가 증가하는 추세이다. 그러나 아직도 이에 대한 연구는 불충분한 실정으로 그라우트 충전식 철근이음 시스템의 보다 적극적인 활용을 위해서는 그 동안 연구의 미비한 부분을 보완하고 개선하여 그라우트 충전식 철근이음의 보다 합리적인 설계방법을 제시하여 실용화시킬 필요가 있다. 이에 본 연구에서는 모르타르 충전식 철근이음에서의 구속효과를 파악하기 위하여 슬리브 표면에 변형률 게이지를 부착한 실물크기의 D25, D19 철근용 스플라이스 슬리브 이음 실험체를 제작한 후에 가력실험을 실시하였다. 이 실험결과로부터 슬리브의 구속효과가 모르타르 충전식 철근이음의 부착성능에 미치는 영향을 고찰하여 다음과 같은 결론을 얻었다. 본 실험에서 측정한 슬리브 표면의 변형률 분포로부터 철근이음에 작용하는 구속력을 산정한 결과, 철근이음 실험체에 최고 $200{\sim}300kgf/{cm}^2$ 이상의 원주방향 구속응력이 작용하였고 이런 구속응력은 철근 정착길이가 감소할수록 커지는 경향이 있었다. 또한 횡방향 구속효과를 고려한 Untrauer와 Merry의 부착강도식에 측정한 슬리브 표면의 변형률로부터 구한 구속응력을 적용하면 본 연구의 실험값을 5% 이내의 편차범위에서 예측할 수 있었다.

• 한국지반공학회논문집
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• 제24권10호
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• pp.147-160
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• 2008

본 논문에서는 국내 몇몇 시험현장에서 수행한 총 54회 단일앵커시험과 4회 실규모 앵커기초에 대한 결과를 제시하였다. 시험결과, 암반앵커의 인발에 대한 파괴메커니즘은 암종 및 암질, 근입깊이, 불연속면의 특성, 텐던의 강도 등에 영향을 받는 것으로 나타났다. 불량한 암반내 얕은 앵커의 경우(정착심도 1.0m 이하) 대부분 그라우트-암반 부착파괴로 나타났으나 이러한 경우에도 깊이를 증가시키면 암반파괴를 유도할 수 있다. 반면에, 얕은 앵커기초라 하더라도 암반상태가 좋으면 부착파괴가 아닌 암반파괴의 형태를 보인다. 한편 실내부착강도 시험결과는 표면부터 진행성파괴가 나타나며 점차 아래로 전파된다. 이때 측정된 텐던-그라우트 부착강도는 그라우트 일축압축강도의 약 $18{\sim}25%$로 나타났으며, 방식 쉬이스로 인한 부착강도의 감소는 보이지 않았다. 연구결과로부터 암반앵커시스템의 인발지지력을 지배하는 주요 파라메터를 결정하고 적용 암반의 분류기준을 제시하였으며, 최종적으로 인발에 대한 암반앵커기초의 지지력을 평가할 수 있는 간편화된 절차를 제안하였다.

• Steel and Composite Structures
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• 제4권5호
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• pp.333-353
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• 2004

Strength of reinforced concrete beams can easily be increased by the use of externally bonded CFRP composites. However, the mode of failure of CFRP strengthened beam is usually brittle due to tension-shear failure in the concrete substrate or bond failure near the CFRP-Concrete interface. In order to improve the ductility of CFRP strengthened concrete beams, critical variables need to be investigated. This experimental and analytical research focused on a series of reinforced concrete beams strengthened with CFRP composites to enhance the flexural capacity and ductility. The main variables were the amount of CFRP composites, the amount of longitudinal and shear reinforcement, and the effect of CFRP end diagonal anchorage system. Sixteen full-scale beams were investigated. A new design guideline was proposed according to the effects of the above-mentioned variables. The experimental and analytical results were found to be in good agreement.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2001년도 봄 학술발표회 논문집
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• pp.63-68
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• 2001

It is now common practice to strengthen reinforced concrete beams by steel plates to their tention surfaces. Such plated beams are designed for flexure using conventional prediction equation and assumption that full bond will be developed between the concrete and the plates. Very advanced design rules have already been developed at the University of Adelaide for adhesive bonding steel plates to reinforced concrete beams in order to prevent premature debonding. This work on plated continuous reinforced concrete beams is to determine experimentally whether these design rules, that were developed for steel plated simply supported beams, could be applied to steel and FRP plated continuous beams. This paper also suggests how to increase the ductility of plated beams.

• 제어로봇시스템학회논문지
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• 제7권5호
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• pp.399-406
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• 2001

This paper presents a dynamic modeling and a sliding mode controller for the high-speed/high-accuracy position control system. The selected target system is the wire bonder assembly which is used in the semiconductor assembly process. This system is a reciprocating one around the pivot point that consists of VCM(voice coil motor) as an actuator and transducer horn as a bonding tool. For the modeling elements, the sys-tem is divided into electrical circuit, magnetic circuit and mechanical system. Each system is modeled using the bond graph method and united into the full system. Two major aims are considered in the design of the controller. The first one is that the horn must track the given reference trajectory. The second one is that the controller must be realizable by using the DSP board. Computer simulation and experimental results show that the designed sliding mode controller provides better performance than the PID controller.

• 한국공작기계학회:학술대회논문집
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• 한국공작기계학회 2000년도 추계학술대회논문집 - 한국공작기계학회
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• pp.83-89
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• 2000

This paper presents a dynamic modeling and a sliding mode controller for the high-speed / high-accuracy position control system. Selected target system is the wire bonder head assembly which is used in semiconductor assembly process. This system is a reciprocating one around the pivot point that consists of VCM(voice coil motor) as a actuator and transducer horn as a bonding tool. For the modeling elements, the system is divided into electrical circuit, magnetic circuit and mechanical system. Each system is modeled by using the bond graph method and united into the full system. Two major aims are considered in the design of the controller. The first one is that the horn must track the given reference trajectory. The second one is that the controller must be realizable by using the DSP board. Computer simulation and experimental results show that the designed sliding mode controller provides better performance than the PID controller.

• 한국세라믹학회지
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• 제12권3호
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• pp.8-12
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• 1975

The crystal structure of synthetic magnesite has been studied by X-ray method. Magnesite is trigonal R3c, with a=4.637$\AA$, c=15.023$\AA$ and Z=6. Intensity data were collected with a Rigaku automated four-circle diffractometer and Mo-K$\alpha$ radiation. The structure was refined by the full-matrix least squares method using anisotropic thermal parameters. The final R index for 234 reflections is 0.037. The C-O and Mg-O bond lengths were 1.283 and 2.105$\AA$, respectively. The interatomic angles of three kinds of O-Mg-O were 88.25, 91.75 and 180.00$^{\circ}$, respectively. It is clarified that the distortion of the Mg-O6 octahedron in magnesite is smaller than that of Ca-O6 in calcite.

• Bulletin of the Korean Chemical Society
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• 제29권5호
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• pp.963-968
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• 2008

For the [$Cr_2(H_2tmp)_2Cl_4$] compound, simplified models with two bridging methoxo ligands have been studied. The influence of the bridging Cr-O-Cr bond angles on the exchange coupling between metal atoms in the model compound has been analyzed by means of density functional calculations with the broken-symmetry approach. Coupling constant calculated for the full structure is in good agreement with the experimentally reported value, confirming the validity of the computational strategy used in this work to predict the exchange coupling in a family of related dinuclear Cr(III) compounds. The calculations indicate a good correlation between the calculated coupling constant and the sum of the squared energy gap of three pairs of metal $t_{2g}$ OMSOs with a limited variation of the Cr-O-Cr angle. The spin density distribution and the mechanism of magnetic coupling interactions are discussed.

• Archives of Pharmacal Research
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• 제19권1호
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• pp.71-73
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• 1996

The molecular structure of pirprofen, 3-chloro-4-(2,5-dihydro-1H-pyrrol-1-yl)-.alpha.-methyl-benzeneacetic acid, was determined by single crystal X-ray diffraction analysis. The compound was recrystallized from a mixture of chloroform and toluene in triclinic, space group P over $\bar1,\; with\; a=4.577(1),\; b=11.213(2),\; C=12.485(2){\AA},\alpa.=107.39(1),\;\beta=97.79(1),\;\gamma=92.03(2),\; and Z=2$ The calculated density is $1.384 g/cm^3$. The structure was solved by the direct method and refined by full matrix least-squares procedure to the final R value of 0.034 for 1681 independent reflections. The non-aromatic dihydropyrrol group is found to be coplanar to the central aromatic ring. The molecules are dimerized through the intermolecular hydrogen bonds at the carboxyl group in the crystal.