• Journal of the korean academy of Pediatric Dentistry
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• v.47 no.4
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• pp.397-405
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• 2020

The aim of this study is to compare cariogenic characteristics of fluoride-sensitive Streptococcus mutans [fluoride-sensitive (FS) S. mutans ] and fluoride-resistant Streptococcus mutans [fluoride-resistant (FR) S. mutans] in the presence of sucrose, and to evaluate its effect on cariogenic biofilm formation. S. mutans ATCC 25175 was continuously cultured in trypticase soy broth (TSB) containing NaF (70 ppm) for 40 days to generate FR S. mutans. FS and FR S. mutans were inoculated in TSB with or without 2% sucrose, and optical density and pH were measured every hour. An oral biofilm was formed using saliva bacteria and analyzed through confocal laser scanning microscopy and CFU count. Finally, the expression of glucosyltransferases genes of both S. mutans was investigated through RT-PCR. FR S. mutans exhibited slower growth and lower acidogenicity in the presence of sucrose compared to FS S. mutans . Both cariogenic and single species biofilm formation was lower in the presence of FR S. mutans, along with reduced number of bacteria. FR S. mutans showed significantly low levels of gtfB, gtfC, and gtfD expression compared to FS S. mutans . On the basis of results, FR S. mutans may be less virulent in the induction of dental caries.

• Journal of the Optical Society of Korea
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• v.13 no.1
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• pp.80-85
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• 2009

An experimental study of energy absorption and x-ray emission from ultrashort laser pulse irradiation of in-situ produced solid clusters has been performed. Silver clusters produced by a 30 mJ, 300 ps laser pulse were irradiated up to an intensity of $3{\times}10^{17}\;W/cm^2$ by a 70 mJ, 45 fs compressed laser pulse from the same Ti:sapphire laser. Absorption of the laser light exceeding 70% was observed, resulting in an x-ray yield (>1 keV) of ${\sim}60{\mu}J$ pulse. This may constitute a much simpler means of intense x-ray generation using ultrashort laser pulses as compared to the irradiation of structured / pre-deposited cluster targets, and it offers higher x-ray conversion efficiency than that from gas clusters and planar solid targets.

• Laser Solutions
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• v.15 no.1
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• pp.15-19
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• 2012

The fabrication of internal diffraction gratings with photoinduced refractive index modification in transparent materials was demonstrated using low-density plasma formation excited by a femtosecond (130 fs) Ti: sapphire laser (${\lambda}_p$=800 nm). The refractive index modifications with diameters ranging from $1{\mu}m$ to $3{\mu}m$ were photoinduced after plasma formation occurred upon irradiation with peak intensities of more than $2.0{\times}10^{13}W/cm^2$. The graded refractive index profile was fabricated to be a symmetric around from the center of the point at which low-density plasma occurred.

• Korean Journal of Optics and Photonics
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• v.26 no.6
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• pp.318-321
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• 2015

We demonstrate a frequency-resolved optical grating (FROG) device for measuring the intensity and phase versus time of several-tens-of-picoseconds laser pulses, using a thick nonlinear optical crystal. The huge pulse-front tilt generated by a holographic grating increases the temporal range of the device, which can make a single-shot measurement of laser pulses less than 100 ps in duration. To verify the measurement technique, we generate double pulses using a Michelson interferometer. The measured duration of a single pulse is about 300 fs and the measured maximum delay of two pulses is 60 ps, which implies that the proposed FROG device can measure laser pulses with maximum pulse width of about 120 ps.

• Journal of the Optical Society of Korea
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• v.13 no.3
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• pp.335-340
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• 2009

Ultrashort pulse laser drilling of polycarbonate track-etched membrane (pTEM) material was used to fabricate a mouse embryo cell trapping device. Holes with a diameter of $2{\mu}m$ to $5{\mu}m$ were fabricated on a $10{\mu}m$ thick membrane using a femtosecond laser with a 150 fs pulse width and 775 nm wavelength and multiple-pulse irradiation. In cell trapping tests, the overall cell occupancy of the machined holes in the fabricated pTEM was found to be more than 80%. The results of a single pulse and multiple pulse irradiation were compared in terms of the surface quality. It was generally found that a single pulse with high energy was less desirable than irradiation with multiple pulses of lower energy.

• Journal of the Optical Society of Korea
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• v.15 no.1
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• pp.56-60
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• 2011

We demonstrate passive mode-locking of a Cr:forsterite laser with a single-walled carbon nanotube saturable absorber mirror (SWCNT-SAM). Without compensation of intra-cavity dispersion, the self-mode-locked laser generates 11.7 ps pulses at a repetition rate of 86 MHz. The dispersion-compensated laser yields ultrashort pulses as short as 80 fs near $1.25\;{\mu}m$ at 78 MHz with average output powers up to 295 mW, representing the highest power ever reported for mode-locked solid-state lasers based on saturable absorption of SWCNTs in this spectral region.

• Laser Solutions
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• v.14 no.1
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• pp.1-5
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• 2011

Photo-induced gratings m flexible PDMS (polydimethly siloxane) film are directly written by a high-intensity femtosecond (130fs) Ti: Sapphire laser (${\lambda}_p$ = 800nm). The refractive index modifications with $4\;{\mu}m$ diameters were photo-induced after the femtosecond irradiation with peak intensities of more than $1{\times}10^{11}W/cm^2$. The graded refractive index profile was fabricated to be a symmetric around the center of the point at which femtosecond laser by controlling both laser power and focused depth. The change on refractive index in the laser-modified regions was estimated to be approximately $10^{-3}$. The internal flexible symmetric diffraction gratings in PDMS film was successfully fabricated using a femtosecond laser.

• Proceedings of the Optical Society of Korea Conference
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• 2009.10a
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• pp.28-29
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• 2009

• Journal of the Korean Society for Precision Engineering
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• v.24 no.12
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• pp.50-56
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• 2007

Femtosecond laser ablation of the Invar alloy and hole drilling for a shadow mask are studied. We used a regenerative amplified Ti-sapphire laser with a 1kHz repetition rate, 184fs pulse duration and 785nm wavelength. Femtosecond laser pulse was irradiated on the Invar alloy with air blowing at the condition of various laser peak power. An ablation characteristic of the Invar alloy was appeared non-linear at $125J/cm^2$ of energy fluence. For the application to a shadow mask, the hole drilling of the Invar alloy with the cross section of a trapezoidal shape was investigated. The ablated micro-holes were characterized using an atomic force microscopy(AFM). The optimal condition of hole pattern f3r a shadow mask was $4\;{\mu}m$ z-axis feed rate, 0.2mm/s circular velocity, $26.4{\mu}J$ laser peak power. With the optimal processing condition, the fine circular hole shape without burr and thermal damage was achieved. Using the femtoseocond laser system, it demonstrates excellent tool for the Invar alloy micro-hole drilling without heat effects and poor edge.

• Laser Solutions
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• v.13 no.1
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• pp.11-15
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• 2010

Indium tin oxide (ITO) provides high electrical conductivity and transparency in the visible and near IR (infrared) wavelengths. Thus, it is widely used as a transparent electrode for the fabrication of liquid crystal displays (LCDs) and organic light emitting diode displays (OLRDs), photovoltaic devices, and other optical applications. Lasers have been used for removing coating on polymer substrate for flexible display and electronic industry. In selective removal of ITO layer, laser wavelength, pulse energy, scan speed, and the repetition rate of pulses determine conditions, which are efficient for removal of ITO coating without affecting properties of the polymer substrate. ITO coating removal with a laser is more environmentally friendly than other conventional etching methods. In this paper, pattering of ITO film from polymer substrates is described. The Yb:KGW femtosecond laser processing system with a pulse duration of 250fs, a wavelength of 1030nm and a repetition rate of 100kHz was used for removing ITO coating in air. We can remove the ITO coating using a scanner system with various pulse energies and scan speeds. We observed that the amount of debris is minimal through an optical and a confocal microscope, and femtosecond laser pulses with 1030nm wavelength are effective to remove ITO coating without the polymer substrate ablation.