• Mycobiology
• /
• v.34 no.1
• /
• pp.38-40
• /
• 2006

In our continuing study on the chemical constituents in the fruiting bodies of Daldinia concentrica, diaporthin and orthosporin were isolated. Their chemical structures were assigned based on various spectral studies. Diaporthin and orthosporin, phytotoxins previously found in Aspergillus ochraceus, were isolated from wood-rotting mushroom D. concentrica for the first time.

• Mycobiology
• /
• v.37 no.2
• /
• pp.155-157
• /
• 2009

Thirty-seven single spore isolates were obtained from specimens of ascomycetous fruiting bodies collected from Mt. Suri, Anyang in Korea. The fungal specimens and isolates were identified as Dumontinia tuberosa based on their morphological and cultural characteristics. This is the first record of this fungus occurring in Korea.

• Journal of Mushroom
• /
• v.7 no.1
• /
• pp.1-8
• /
• 2009

Thirty strains of genus Agrocybe which had been kept in National Institute of Horticultural and Herbal Science, were tested for the formation of fruiting body. Nineteen strains of collected genus Agrocybe were formed fruiting body at bottle culture of sawdust medium. Five strains (including ASI 19003) and thirteen strains (including ASI 19007) could be grouped as A. cylindracea and A. chaxingu. Although ASI 19008 showed the formation of fruiting body, morphological characteristics were significantly different from the two groups. Cultural period of ASI 19003 strain at sawdust substrates was 29 days and ASI 19007 train was 30 days in liquid spawn inoculation. The yield and quality of ASI 19003 strain was excellent in the spring, fall, and winter, whereas ASI 19007 strain was excellent in the summer. Accordingly, these cultivar might be contribute to farmers' income by stable year-round production if using the season-oriented strain.

• Korean Journal of Microbiology
• /
• v.37 no.4
• /
• pp.239-244
• /
• 2001

We isolated a Myxobacteria strain from a soil sample obtained from Mt. Daedoon located in Choongnam, Korea. This strain, ARJ, secreted slime while swarmed on the surface of CT medium. It produced greenish yellow pigment in liquid or solid media, and the swarming edge showed green florescence under U. V. at 366 nm. It formed fruiting bodies when nutrient was exhausted, which is one of the most imkportant characteristics of Myxobacteria. The fruiting bodies did not have a stalk and consisted of naked myxospores when examined under the scanning electron microscope. These traits lead us to believe that this strain is very close to Myxococcus virescens. It showed antimicrobial activity, especially against Gram positive bacteria. Culture filtrate showed the activity but this was not due to protein. The culture filtrate also had proteolytic activity in which at least two enzymes are involved.

• Mycobiology
• /
• v.36 no.1
• /
• pp.13-18
• /
• 2008

Eight distinct bacteria were isolated form diseased mycelia of the edible mushroom, Pleurotus eryngii. 16S rDNA sequence analysis showed that the isolates belonged to a variety of bacterial genera including Bacillus (LBS5), Enterobacter (LBS1), Sphingomonas (LBS8 and LBS10), Staphylococcus (LBS3, LBS4 and LBS9) and Moraxella (LBS6). Among them, 4 bacterial isolates including LBS1, LBS4, LBS5, and LBS9 evidenced growth inhibitory activity on the mushroom mycelia. The inhibitory activity on the growth of the mushroom fruiting bodies was evaluated by the treatment of the bacterial culture broth or the heat-treated cell-free supernatant of the broth. The treatment of the culture broths or the cell-free supernatants of LBS4 or LBS9 completely inhibited the formation of the fruiting body, thereby suggesting that the inhibitory agent is a heat-stable compound. In the case of LBS5, only the bacterial cell-containing culture broth was capable of inhibiting the formation of the fruiting body, whereas the cell-free supernatant did not, which suggests that an inhibitory agent generated by LBS5 is a protein or a heat-labile chemical compound, potentially a fungal cell wall-degrading enzyme. The culture broth of LBS1 was not inhibitory. However, its cell-free supernatant was capable of inhibiting the formation of fruiting bodies. This indicates that LBS1 may produce an inhibitory heat-stable chemical compound which is readily degraded by its own secreted enzyme.

• Mycobiology
• /
• v.36 no.2
• /
• pp.81-87
• /
• 2008

The optimal conditions for mycelial growth of Phellinus linteus ATCC 26710 were determined to be a log length of 20 cm, temperature of $30^{\circ}C$ and pH of 6.0. Mycelial growth was excellent on the mushroom complete medium, and was optimal when sucrose, man nose and glucose were supplied as carbon sources. Potassium nitrate and sodium nitrate as nitrogen sources supported good mycelial growth. To evaluate P. linteus mycelial colonization on logs, sterilized short log inoculation, drilling inoculation and log-end sandwich inoculation techniques were used. Only sterilized short log inoculation produced good mycelial colonization. Initial mycelial growth and full mycelial colonization were best on 20 cm logs having 42% moisture content. The initial mycelial growth of P. linteus was accelerated over 12hr of sterilization. Basidiocarp formation was optimal using a burying method of logs after $5{\sim}6$ months, and fruiting body formation was superior in cultivation house conditions of $31{\sim}35^{\circ}C$ and in excess of 96% relative humidity.

• Mycobiology
• /
• v.38 no.1
• /
• pp.52-57
• /
• 2010

To develop a potent anti-obesity lipase inhibitor from mushroom, the lipase inhibitory activities of various mushroom extracts were determined. Methanol extracts from Phellinus linteus fruiting body exhibited the highest lipase inhibitory activity (72.8%). The inhibitor was maximally extracted by treatment of a P. linteus fruiting body with 80% methanol at $40^{\circ}C$ for 24 hr. After partial purification by systematic solvent extraction, the inhibitor was stable in the range of $40\sim80^{\circ}C$ and pH 2.0~9.0. In addition to lipase inhibitory activity, the inhibitor showed 59.4% of superoxide dismutase-like activity and 56.3% of acetylcholinesterase inhibitory activity.

• Korean Journal of Pharmacognosy
• /
• v.39 no.4
• /
• pp.365-368
• /
• 2008

Recently proteasome inhibitors have been emerged as potential anticancer agents. In a continuous study on exploring proteasome inhibitors from natural products, fruiting body of Hericium erinaceum was investigated. The MeOH extracts of the fruiting body of Hericium erinaceum was fractionated with several solvents and the fractions were evaluated on the activity to screen the proteasome inhibitors. The n-Hexane and CHCl3 frs. showed potent activity, of which chemical investigations led to ergosterol peroxide (1), hericenones C (2) and D (3). Their structures were determined by spectroscopic methods such as $^1H$-NMR, $^{13}C$-NMR, and FABMS spectra.

• Food Science and Biotechnology
• /
• v.15 no.2
• /
• pp.308-311
• /
• 2006

Saesongi (Pleurotus eryngii) was cultivated in both potato dextrose agar (PDA) and sawdust media supplemented with Ca salts. The addition of Ca phosphate and Ca carbonate to sawdust media did not affect the growth, whereas Ca sulfate addition suppressed the mycelial growth appreciably. The efficiencies of Ca accumulation in the fruiting were studied based on mycelial growth experiments on Ca-supplemented sawdust media. Supplementation with 0.1 to 5% Ca phosphate increased the Ca content in the fruiting body by 4.5-6.5 fold, to a level of $314.6{\pm}22.7$ to $449.7{\pm}29.3$.

• Journal of the Korean Wood Science and Technology
• /
• v.38 no.5
• /
• pp.439-443
• /
• 2010

The 95% aqueous EtOH extract was obtanied from the fruiting body of Russula subnigricans. Repeated silica gel column chromatography and preparative TLC afforded one fatty acid and three chlorinated hydroquinone derivatives. They were identified as nonadecanoic acid (1), 2,6-dichloro-4-methoxyphenol (2), russuphelin A (3), and russuphelin E (4) on the basis of several spectral data (MS, $^1H$ and $^{13}C$-NMR, including HMBC).