• 농업과학연구
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• 제40권2호
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• pp.93-99
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• 2013

The disease and insect were surveyed locally in greenhouse, fruit packing house and store house of 51 farms in 13 towns having purpose of paprika exportation. By analysis, various disease and insect were not only founded locally but more ones detected in farms having old facilities and no natural enemy. We found 15 pathogens such as Fusarium spp., Alternaria solani, Leveilluila taurica, PepMV (Pepino mosaic virus) and TMV (Tobacco mosaic virus) in greenhouse, Fusarium spp. in fruit packing house and Penicillium spp. in store house. We found 15 insects in greenhouse such as Bemisia tabaci, rialeurodes vaporariorum and Myzus persicae in greenhouse, Hylobitelus haroldi in fruit packing house. However, the problem quarantine disease and insect for importation and exportation were not detected in inspection time.

• Mycobiology
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• 제38권2호
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• pp.113-117
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• 2010

Trichoderma strains were evaluated under field conditions to assay their efficacy in suppressing Alternaria fruit rot disease and promoting chili plant growth. The experiment was conducted at the Botanical Garden, Rajshahi University, Bangladesh from July 2006 to March 2007. Application of Trichoderma harzianum IMI 392432 significantly (p = 0.05) suppressed the disease compared to Alternaria tenuis (T2) treatment and improved both growth and yield. The treatment T4 (T. harzianum IMI-392432 + A. tenuis) was most effective in reducing disease percentage (72.27%) compared to A. tenuis (T1) treatment. The highest seed germination rate (85.56%) and the highest growth and yield (12.5 g/plant) was also recorded in the same treatment (T4), followed by T5 (T. harzianum IMI-392433 + A. tenuis), T6 (T. harzianum IMI-392434 + A. tenuis), T2 (T. virens IMI-392430 + A. tenuis), and T3 (T. pseudokoningii IMI-392431 + A. tenuis) treatment, while single treatment with A. tenuis significantly decreased these values.

• The Plant Pathology Journal
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• 제40권2호
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• pp.205-217
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• 2024

Brown rot disease, caused by Monilinia spp., poses a significant threat to pome and stone fruit crops globally, resulting in substantial economic losses during pre- and post-harvest stages. Monilinia fructigena, M. laxa, and M. fructicola are identified as the key agents responsible for brown rot disease. In this study, we employed the amplified fragment length polymorphism (AFLP) method to assess the genetic diversity of 86 strains of Monilinia spp. isolated from major stone fruit cultivation regions in South Korea. Specifically, strains were collected from Chungcheong, Gangwon, Gyeonggi, Gyeongsang, and Jeolla provinces (-do). A comparative analysis of strain characteristics, such as isolation locations, host plants, and responses to chemical fungicides, was conducted. AFLP phylogenetic classification using 20 primer pairs revealed the presence of three distinct groups, with strains from Jeolla province consistently forming a separate group at a high frequency. Furthermore, M. fructicola was divided into three groups by the AFLP pattern. Principal coordinate analysis and PERMANOVA were applied to compare strain information, such as origin, host, and fungicide sensitivity, revealing significant partition patterns for AFLP according to geographic origin and host plants. This study represents the utilization of AFLP methodology to investigate the genetic variability among M. fructicola isolates, highlighting the importance of continuous monitoring and management of variations in the brown rot pathogen.

• 한국식물병리학회지
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• 제10권3호
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• pp.169-174
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• 1994

A new unidentified pear leaf spot disease presumed to first occur in the late 1970's has recently become prevalent over the pear growing areas, and caused the greatest problem for pear production in Korea. The disease began to develop on pear leaves at mid- to late May, peaked at mid- to late une, but stopped further development until September in cool climate. Leaf lesions are 0.9∼2.5 mm in diam., oval or irregular to rectangular in shape, first appeared reddish purple, later changed to dark brown, and to whitish grey in the late season. Lesions were limited to appear only on the mature, hardened leaves, initially from leaf margin or near the leaf veins, and later scattered over the leaf surface. Individual lesions usually did not enlarge, but often coalesced each other, commonly causing shot holes and eventual early falling. The disease was most severe on the major pear cultivars Niitaka and Okusankichi ranged with 4 to 100% infections in trees, depending on the orchards, but not on the cultivar Chojuro. Damages from the disease included lower fruit weight, and higher acid and less sugar content in fruits, resulting in lowering the overall fruit quality. Etiology of the disease including identification of the causal organism is in a separate paper.

• The Plant Pathology Journal
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• 제34권6호
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• pp.480-489
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• 2018

Bitter rot caused by Colletotrichum species is a common fruit rotting disease of apple and one of the economically important disease in worldwide. In 2015 and 2016, distinct symptoms of bitter rot disease were observed in apple orchards in five regions of South Korea. In the present study, infected apples from these regions were utilized to obtain eighteen isolates of Colletotrichum spp. These isolates were identified and characterized according to their morphological characteristics and nucleotide sequence data of internal transcribed spacer regions and glyceraldehyde-3-phosphate-dehydrogenase. Molecular analyses suggested that the isolates of Colletotrichum causing the bitter rot disease in South Korea belong to 4 species: C. siamense; C. fructicola; C. fioriniae and C. nymphaeae. C. siamense and C. fructicola belonged to Musae Clade of C. gloeosporioides complex species while C. fioriniae and C. nymphaeae belonged to the Clade 3 and Clade 2 of C. acutatum complex species, respectively. Additionally, we also found that the isolates of C. gloeosporioides species-complex were more aggressive than those in the C. acutatum species complex via pathogenicity tests. Taken together, our results suggest that accurate identification of Colletotrichum spp. within each species complex is required for management of bitter rot disease on apple fruit in South Korea.

• 식물병연구
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• 제24권2호
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• pp.113-122
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• 2018

Ripe rot, Botrytis storage rot and Sclerotinia rot are major fungal diseases that lead to deterioration of fruit quality in Korea. Ripe rot, which is caused by Botryosphaeria dothidea, affects harvested fruits during post-storage ripening, while Botrytis storage rot, caused by Botrytis cinerea, affects harvested fruits during cold storage, and Sclerotinia rot, caused by Sclerotinia sclerotiorum, mainly affects immature fruits on the trees. Major fungal fruit rots tend to affect yellow- and red-fleshed cultivars of kiwifruit more severely because of the sharp increase in their cultivation acreage in recent years in Korea. In this review, we summarize symptoms and epidemiological characteristics of the major fungal fruit rots and propose integrated management methods of the diseases that can be practically utilized at the farmers' orchards in order to prevent the diseases based on our research works and field experiences and the research works of others conducted during the last three decades worldwide.

• 한국식물병리학회지
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• 제11권1호
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• pp.98-99
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• 1995

A disease causing fruit rot and leaf and vine blight on watermelon was found in Euseong in July, 1991 and in Bonghwa, Kyungpook province in August, 1993. Abundant zoosporangia characteristics of Phytophthora capsici were formed on the surface of the infected fruits. The zoosporangia were ovoid to ellipsoid, tapering to the base and with conspicuous papilla. The fungus isolated from the infected fruits was pathogenic on seedlings of watermelon, pumpkin, and pepper. The morphological characteristics and host range agreed with those of P. capsici. The watermelon disease caused by P. capsici was reported long time ago in Japan and America but rot yet in Korea. Thus, the disease on watermelon caused by P. capsici is reported as a new record in Korea.

• 한국식물병리학회지
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• 제10권2호
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• pp.99-104
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• 1994

A computer-based diagnosing system for diseases of grasses, ornamental plant and fruit trees was developed using a 16 bit personal computer (Model Acer 900) and BASIC was used as a programing language. the developed advisory system was named as Korean Plant Disease Advisory System (KOPDAS). The diagraming system files were composed of a system operation file and several database files. The knowledge-base files are composed of text files, code files and implement program files. The knowledge-base of text files are composed of 79 files of grasses diseases, 122 files of ornamental plant diseases and 67 files of fruit tree diseases. The information of each text file include disease names, causal agents, diseased parts, symptoms, morphological characteristics of causal organisms and control methods for the diagnosing of crop diseases.

• 한국약용작물학회지
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• 제28권6호
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• pp.435-444
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• 2020

Background: As the Positive List System (PLS) is implemented in broad application to agricultural products, attention to the correct use of pesticides is also needed in the production of mulberry leaves and fruit. In this regard, three types of pesticides against mulberry popcorn disease were applied 2 - 3 times both in the field and greenhouses to prepare safety standards. Residual pesticide analysis was conducted on mulberry fruits and leaves. Methods and results: Three pesticieds, thiophanate-methyl, thiophanate-methyl·triflumizole and fluopyram registered as PLS pesticides for mulberry popcorn disease, were sprayed in the Wanju and in Buan regions, after which residual pesticide analysis was conducted using high-performance liquid chromatography (HPLC). As three pesticieds were either undetected or below the permissible level in mulberry fruit, demonstrating that they were suitable for safe spraying. However, 5.6 mg/kg of thiophanate-methyl was detected in the greenhouse after three application, which was slightly above maximum residue limit (MRL). Furthermore the level of thiophanate-methyl·triflumizole was higher than 0.2 mg/kg ("Gwasang No. 2" variety, spraying twice) or similar to 0.09 mg/kg ("Daesim" variety, spraying thrice) the permissible level (0.1T mg/kg) as the thiophanate-methyl was detected in mulberry leaves in the greenhouse. Conclusions: The spraying frequency for controlling mulberry popcorn disease in greenhouses should be limited to two times or less, especially when mulberry leaves are treated with thiophanate-methyl·triflumizole careful consideration is required if the leaves are to be used as food materials.

• 식물병연구
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• 제10권4호
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• pp.313-318
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• 2004

우리나라에서 오이 속썩음은 대전을 비롯한 여러 오이 재배지역에서 발생하였다. 본 병의 발생은 조사포장에서 평균 4.2%, 최대 21.5%까지 발생하였다. 병 발생은 오이 꽃이 붙어 있는 부위부터 시작되었다. 병원균을 접종하여 감염된 오이의 내부조직은 길이 2cm, 폭이 2mm 이상으로 갈변되었다. 후에 갈변은 진전되어 과일의 심피에 이르렀으며, 감염된 오이의 끝부분이 울퉁불퉁하게 되었다. 병반부위에서 분리된 병원균은 형태적 특징에 의하여 Didymella bryoniae으로 동정되었다. 병원균의 균사생장 온도범위는 $5{\sim}32^{\circ}C$이고, 최적 균사생장 온도는 $26{\sim}28^{\circ}C$이었다. 포자현탁액을 오이 꽃에 접종하여 오이 과일의 내부에서 자연감염된 것과 유사한 병징이 나타났다. 오이, 수박, 참외, 멜론 및 호박에서 분리된 D. bryoniae균주들은 접종실험에 의하여 오이 속썩음이 나타났다. 오이 속썩음의 발생 온도범위는 $10{\sim}32^{\circ}C$이며, 최적 발생온도는 $25{\sim}28^{\circ}C$이었다.