• Korean Journal of Medicinal Crop Science
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• v.25 no.6
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• pp.404-410
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• 2017

Background: Hair loss related syndromes are increasing due to environmental pollution and stress. Hair care products are mainly prepared by mixing chemicals and natural extracts, such as those obtained from medicinal plants. The purpose of this study was to investigate the effects of 70% ethanol extracts from the flowers of Calendula officinalis, fruit body of Phellinus linteus, and the whole plant of Houttuynia cordata on the growth of CCD-986 cells, hair follicle dermal papilla cells (HFDPC), and 3T3-L1 cells. Methods and Results: All sample extracts at all concentrations, except for that from P. linteus fruit body at $500{\mu}g/m{\ell}$, were cytotoxic to CCD-986 cells. However, none of the sample extracts were cytotoxic to HFDPC. The lipid differentiation of 3T3-L1 cells regulates hair regeneration via secretion of platelet derived growth factor. The 70% ethanol extract of H. cordata whole plant promoted hair growth. Adipogenesis rate significantly increased in a treatment concentration-dependent manner. Conclusions: These results suggest that 70% ethanol extracts of C. officinalis flower, P. linteus fruit body and H. cordata could be used for the development of hair care products.

• Journal of Practical Agriculture & Fisheries Research
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• v.22 no.1
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• pp.99-112
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• 2020

A edible mushroom, Hypsizygus marmoreus is commercially cultivated. However, the researches of cultivation and physiological characteristics were not conducted in Korea. In this study, we conducted on artificial cultivation of H. marmoreus and elucidated the effect of light on the mycelial growth and fruit body development using LED light sources with different wavelength; blue (peak wave length 460nm), green(peak wave length 530nm), yellow(peak wave length 590nm), red(peak wave length 630nm), and white as positive control. Mycelial growth of H. marmoreus strains were inhibited about 30~40% in inhibition ratio under the illumination with blue, green, yellow LED light. However, red LED light was not inhibited. Elongation of stipe was effective under the long wave length such as yellow and red light. Abnormal fruit body was produced under the long wavelength and dark. However, development of pileus was effective under the short wavelength such as green and blue light. Also, as a result of cultivation with mixed light for high quality and harvest, many effective numbers and yields of fruiting bodies were obtained in the mixed treatment of blue and white light, and pileus developed well.

• Journal of Mushroom
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• v.11 no.1
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• pp.21-23
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• 2013

We aim at providing consumers and farmers with various parts of mushrooms through development of new mushroom cultivation. For the mass artificial cultivation, we elucidated suitable environment conditions of Neolentinus lepideus. The results were summarized as follows. For the efficient cultivation of N. lepideus, temperature of mycelial growth and development of the fruit body were suitable $20{\sim}23^{\circ}C$ and $20^{\circ}C$, respectively.

• The Korean Journal of Mycology
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• v.36 no.1
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• pp.51-57
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• 2008

To develop artificial cultivation and improve some problems such as auto-lysis on commercialization of Coprinus comatus that has been known edible and medicinal mushroom, they were conducted for selection of superior strain, suitable culture methods for mycelial growth and fruiting, and morphological characteristics of fruit body. Strain CM 980301 of Coprinus comatus was selected as a superior strain for artificial cultivation. Wheat grain and rice straw full-grown compost media were most effective for preparation of spawn and artificial cultivation of C. comatus, respectively. Spawn running of Coprinus spp. on the rice straw full-grown compost media required to be 15 days from 24 to $28^{\circ}C$. The casing layer incubation before initiation of fruit body formation, required for 13 days at same temperature for spawn running. And then require $10{\sim}11$ days for initiation and $7{\sim}8$ days for development of fruit body from 20 to $24^{\circ}C$. The fruit body of strain CM 980301 was harvested within a week from initiation of primordium formation. The hardness of pileus and stipe that were harvested in optimal stage showed 102 to 169, and 128 to $182\;g/cm^2$, respectively. Yields of srain CM 980301 from the rice straw full-grown compost media was $37.7kg/3.3m^2$. Weight of individual fruit body was 17.9 g in average.

• The Korean Journal of Food And Nutrition
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• v.33 no.4
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• pp.363-371
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• 2020

This study was designed to evaluate the improvement in exercise endurance by Hovenia dulcis fruit hot water extract (HDWE) for the potential application as a functional food material. The effect was based on the evaluation of the forced-swimming capacity and changes in biochemical parameters in mice. Six-week-old mice were classified into four groups based on a randomized block design: the negative control group, the HDWE 50 mg/kg body weight group, the HDWE 200 mg/kg body weight group, and the 1-octacosanol 50 mg/kg body weight group (n=8 mice per group). The swimming exercise time in the HDWE-50 group was increased at 545±43 sec compared to the control group at 263±28 sec. The HDWE-200 fatigue indicators, lactate acid and ammonia content, decreased to 4.99±0.33 mg/mL and 0.63±0.05 mg/mL, respectively. In addition, the content of glycogen in the liver and muscle tended to increase in the group administered HDWE but was not significant. Likewise, the LDH and glucose contents were increased compared to the control group. The muscle damage indicators ALT (52.7±2.6 U/L) and AST (42.5±5.2 U/L) tended to decrease compared to the control group (76.4±10.2 U/L).

• Journal of Microbiology and Biotechnology
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• v.19 no.8
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• pp.774-780
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• 2009

Flammulina velutipes is a popular edible basidiomycete mushroom found in East Asia and is commonly known as winter mushroom. Mushroom development showing dramatic morphological changes by different environmental factors is scientifically and commercially interesting. To create a genetic database and isolate genes regulated during mushroom development, cDNA libraries were constructed from three developmental stages of mycelium, primordium, and fruit body in F. velutipes. We generated a total of 5,431 expressed sequence tags (ESTs) from randomly selected clones from the three cDNA libraries. Of these, 3,332 different unique genes (unigenes) were consistent with 2,442 (73%) singlets and 890 (27%) contigs. This corresponds to a redundancy of 39%. Using a homology search in the gene ontology database, the EST unigenes were classified into the three categories of molecular function (28%), biological process (29%), and cellular component (6%). Comparative analysis found great variations in the unigene expression pattern among the three different unigene sets generated from the cDNA libraries of mycelium, primordium, and fruit body. The 19-34% of total unigenes were unique to each unigene set and only 3% were shared among all three unigene sets. The unique and common representation in F. velutipes unigenes from the three different cDNA libraries suggests great differential gene expression profiles during the different developmental stages of F. velutipes mushroom.

• Journal of Mushroom
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• v.12 no.4
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• pp.258-262
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• 2014

We aim to elucidate suitable mixed color of light during development of fruit body in Pleurotus ostreatus. The four mixed color of LED(Light Emitting Diode), blue and white, green and white, blue and green and green and red LED, were irradiated for formation of fruit-body. First, as effect of mixed color of LED, the properties of fruit body of P. ostreatus in blue and white LED irradiation were showed higher commercial yields than those in control(fluorescent lamp). And the ergothioneine was the highest at the blue and white LED. Second, we could obtain higher commercial yields than those at the $1{\mu}mol/m^2/s$ of light intensity by blue and white LED in P. ostreatus.

• Journal of Mushroom
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• v.9 no.1
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• pp.34-38
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• 2011

In this study, temperature and relative humidity during growth of fruit body were applied to oyster mushroom (Pleurotus ostrestus) to elucidate the prolongation effect of storage. Although there were not big differences among conditions of cultivation, weight loss and change of pileus color were slight increased and hardness and springness of fruit body were little decreased with storage period. When whole mushrooms (250~300g) were packaged with wrap and stored at $4{\pm}1^{\circ}C$, the shelf life of the oyster mushrooms were cultivated at $16^{\circ}C$ and at $13^{\circ}C$ was 18 day and 24days, respectively. Therefore, it was elucidated that the treatment of low-temperature at the step of fruit body development affected extension of shelf life in oyster mushroom.

• Journal of Mushroom
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• v.11 no.4
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• pp.226-229
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• 2013

This study was carried out to elucidate suitable wavelength of light during development of fruit body in Lentinula edodes. The four colors of LED(Light Emitting Diode), blue, green, red and yellow, were irradiated for formation of fruit-body. The effect of color of LED at all growth stage, the lightness of cap showed darker in blue and green LED irradiation than that of red and yellow LED. The longer stipe were resulted in longer wavelength. And the activity of anti-oxidant did not showed big differences according to LED wavelength. We obtained higher commercial yields and lower ratio of abnormal fruit body in green LED than those in control(fluorescent lamp).

• Journal of Mushroom
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• v.9 no.3
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• pp.101-104
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• 2011

We aimed to develop a new variety of Neolentinus lepideus from about forty strains by bag culture. To this end, "Solhyang" which means pine smell in Korean was selected as a new commercial variety of N. lepideus. N. lepideus have yellow pileus and pine smell, which characteristics make a favorable impression on the far east Asia, Korean and Japanese. The optimum temperatures for mycelial growth and fruit body development ranged $26{\sim}32^{\circ}C$ and $18{\sim}20^{\circ}C$, respectively. The required periods of mycelial incubation and fruit body growth were 30 days and 7 days, respectively. The fresh weight of fruit body was 115g/kg with pine sawdust and corn meal power(9:1, v/v) substrate.