• Journal of Acupuncture Research
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• 제18권2호
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• pp.51-66
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• 2001

The purpose of this morphological studies was to investigate the central neural pathway projecting to the acupoints $B_{62}$ and $K_6$ using the neuroanatomical method following injection of transsynaptic neurotropic virus, pseudorabies virus(PRV-Ba and PRV-Ga) into the $B_{62}$ and $K_6$. After survival times of 96 hours following injection into the twenty rats with PRV-Ba(Bartha strain) and PRV-Ga(Bartha strain, ${\beta}$-galacidodase insertion). They were perfused, and their spinal cord and brain were frozen sectioned($30{\mu}m$). These sections were stained by X-gal histochemical and PRV immunohistochemical staining method, and observed with light microscope. The results were as follows : 1. In spinal cord, overlaped PRV-Ba and PRV-Ga labeled neurons projecting to the $B_{62}$ and $K_6$ were founded in thoracic, lumbar and sacral spinal segments. In thoracic spinal segments, Densely labeled areas were founded in lamina IV, V, VII(intermediolateral nucleus) and X areas. In lumbar segemnts, labeled areas were founded in lamina II, IV, V and X areas. In sacral spinal segments, labeled areas were founded in lamina IV, V and VI areas. 2. In brain, overlaped PRV-Ba and PRV-Ga labeled neurons projecting to the $B_{62}$ and $K_6$ were founded in the $A_1$ noradrenalin cells/$C_1$ adrenalin cells/caudoventrolateral reticular nucleus, rostroventrolateral reticular nuclens, nucleus tractus solitarius, area postrema, raphe obscurus nucleus, raphe paltidus nucleus, raphe magnus nucleus, lateral paragigantoceltular nucleus, lateral rcticular nucleus, gigantocellular nucleus, locus coeruleus, subcoeruleus nucleus, motor trigeminal nucleus, Kolliker-Fuse nucleus, $A_5$ cell group, central gray matter, oculomotor nerve, paraventricular hypothalamic nucleus, median eminence, amygdaloid nucleus, frontal cortex, forelimb area, hindlimb area, 1, 2 areas of parietal cortex and granular and agranular cortex. This results were suggest that overlaped PRV-Ba and PRV-Ga labeled areas projecting to the $B_{62}$ and $K_6$ may be related to the emotional relay pathway in the central autonomic center.

• Journal of Acupuncture Research
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• 제31권1호
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• pp.131-137
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• 2014

Objectives : This study is to investigate the effects of Acanthopanacis Cortex hot aqueous extract on nitric oxide(NO), prostaglandin E2(PGE2) production and DPPH(1,1-diphenyl-2-picryl hydrazyl) radical scavenging activity in macrophages. Methods : Acanthopanacis Cortex(200 g) was heated at $100^{\circ}C$ with distilled water(2 L) for 4hrs. The extract was filtered and concentrated to 100 ml using a rotary evaporator and was frozen at $-80^{\circ}C$, then was freeze-dried. The RAW 264.7 macrophages were subcultured. In order to evaluate cytotoxicity, MTT assay was performed. Experimental groups were divided into five(control, AC 25, 50, 100 and 200 ${\mu}g/ml$) and we measured cytotoxicity. The concentrations of NO were preprocessed by Griess assay. The RAW 264.7 macrophages was pretreated by 10 ${\mu}g/ml$ LPS and experimental groups were divided into five and we measured NO production. The concentrations of $PGE_2$ were measured by enzyme immunoassay. The RAW 264.7 macrophages was pretreated by 10 ${\mu}g/ml$ LPS. Experimental groups were divided into five and we measured $PGE_2$ production. Antioxidant activity was measured by the DPPH method. experimental groups were divided into four(AC 25, 50, 100 and 200 ${\mu}g/ml$) and we measured DPPH radical scavenging activity. Results : 1. Viability of RAW 264.7 macrophages did not significantly decrease in 25, 50 and 100 ${\mu}g/ml$ Acanthopanacis Cortex hot aqueous extract compared to control group. 2. NO production in LPS-stimulated RAW 264.7 macrophages significantly inhibited in 100, 200 ${\mu}g/ml$ Acanthopanacis Cortex hot aqueous extract compared to control group. 3. $PGE_2$ production in LPS-stimulated RAW 264.7 macrophages significantly inhibited in 100, 200 ${\mu}g/ml$ Acanthopanacis Cortex hot aqueous extract compared to control group. 4. DPPH radical scavenging capability of Acanthopanacis Cortex hot aqueous extract in RAW 264.7 macrophages had the high level in 100, 200 ${\mu}g/ml$. Conclusion : According to the results, Acanthopanacis Cortexx hot aqueous extract has ability to suppress NO, $PGE_2$ production and improve DPPH free radical scavenging activity. So Acanthopanacis Cortex hot aqueous extract may have an anti-inflammation effect and antioxidant activity.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2015년도 추계학술대회
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• pp.190-193
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• 2015

현재 나와 있는 전자해도 기반의 항로표지 시스템은 ECDIS에 사용하기 위한 데이터 제작 사양만 가지고 있기 때문에 근본적으로 여러 수로데이터 표현에는 한계를 가지고 있으며, 장기간 수정 불가하도록 동결되어 있으므로 생산성이 떨어진다. 또한 격자구조 자료, 시계열 정보 같은 첨단 요구 사항을 만족시킬 수 없는 단점이 있다. 현재 S-57을 기반으로 각기 독립적인 운용체계를 구축하고 있지만 모든 프로토콜의 규격화와 포맷의 통일로 전체 모니터링 시스템이 상호연동이 될 수 있는 방안이 수요자의 가장 큰 요구사항인 것으로 파악 되었다. 또한 시중에 나와 있는 상태정보 안내 및 경보 시스템은 AIS, TRS, WCDMA 통신을 하여 각각 다른 서버에 저장을 한 후에 또 다른 서버를 이용하여 데이터를 모아 처리 하는 방식으로 나와 있다. 개발한 시스템의 성능 테스트를 위하여 현재 사용되고 있는 등명기를 이용하고 생성된 데이터들을 RS-232를 통하여 전송하였고, 상태 모니터링 시스템이 데이터를 수신 되는 것을 확인하였다. 또한 이 데이터를 데이터베이스에 넣어 외부 서버로 전송 했을 때 같은 데이터가 정상적으로 수신 되는 것을 확인 하였다. 본 논문에서는 각 다른 프로토콜을 통합하여 통합시스템을 만들고 S/W인 S-63 전자해도 기반으로 된 항로 표지 시스템인 해양교통시설의 상태정보 안내 및 경보시스템을 구현하였다.

• 한국응용곤충학회지
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• 제46권3호
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• pp.437-444
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• 2007

원산지가 열대지방인 왕우렁이가 국내에서는 월동이 불가능할 것으로 여겨졌으나 1980년대 초에 도입된 후 20여년이 지난 현재 한국 기후에 적응되어 남부지방의 일부 지역에서 월동이 확인되었다. 본 연구는 2003년부터 2006년까지 영남지역에서 왕우렁이를 이용하여 벼 잡초방제를 실시한 지역을 중심으로 왕우렁이의 월동분포와 월동지역의 환경특성을 조사하기 위하여 수행하였다. 영남지역의 왕우렁이 월동실태를 조사한 결과, 하동, 합천, 산청, 김해, 밀양, 창원, 울산, 부산, 경주, 포항, 칠곡, 구미, 상주 및 영주에서 월동을 확인하였는데, 월동지역은 해마다 북상하였다. 왕우렁이가 월동되는 지역의 환경을 조사한 결과 주로 깊은 웅덩이나 겨울에도 물이 계속 흐르는 하천이나 수로, 지하수가 계속 흘러내리는 수로 및 수막재배를 하는 비닐하우스 단지 주변의 도랑 등이다. 이들 장소들의 특징은 겨울에도 수온이 크게 내려가지 않아 물이 얼지 않거나 표면에만 물이 얼고 속에는 물이 얼지 않는 장소들이었다.

• 수산경영론집
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• 제1권2호
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• pp.1-22
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• 1970

Our fisheries protucts industry has developed rapidly during past 10 years ; production was about twice: fihing fleets increased twice in number and 3 times in tonnage ; export was 5 times. Govermment is trying to develop deep sea fisheries in order to surmount the depression of coast fisheries. At present more than 270 deep sea fishing boats are working with superior skill to other country at the South Pacific, the Atlantic, the Indian, and the North Pacific Ocean. Our deep sea fisheries is consisted of the tuna long line and the trawler. The tuna long line of them has 230 crafts in 1969 ana the deep sea trawler has 40 crafts, too. Comparing to 1962, the number of the deep sea fishing boats has been increased highly to 54 times, 7.71 times average per year increasing rate. The rate of the tuna long line to the trawler at the end of 1969 shows 85:15, More than half of them are 100~200 (equation omitted), if we classify them according to (equation omitted) or boat craft. 70% of them has less than 5 years ships age. The Korean Marine Industry Development Corporation has more than 1/3 fishing fleets, with 91 crafts, if We consider it according to corporation. Considering it according to the financial resources, dependence upon foreign loan is as high as 88%. Catches was 74, 450 M/T ($24, 663, 000)at the end of November in 1969 and it was increased to 113.5 times in catch amount and 118 times in value, comparing to those of 1962. Considering it according to the ocean, the order is arranged to the Pacific, the Atlantic, and the Indian Ocean. The average production amount of each craft is 250~400 S/T a year. The result of export took up 1/3 of total fisheries product export with $ 22, 398, 000 at the end of november in 1969. Employee cost of fishing coast is 8% higher than other fishing. The profit is highest in our fisheries. Most of the products except the trawler fishing are sofa at the fishing grounds to the processing company, and they lose much money. They buy most of bait from Japan, giving $8~10 for 1C/S(10kg). Fish price is $390~520 according to the kind of fish for S/T at the fishing grounds, and the rapid frozen fishes brought to Japan are about $ 800 for S/T. There is much difference. in price. Problems 1. Want of self capital. 2. To get the refrigeration boats enough. 3. International Fishing Regulation. 4. To get high price and to secure consuming grounds. 5. To get home-production of bait. 6. To exploit new fishing grounds. 7. To get larger boats. 8. To get mariner enough. When the problems mentioned above are solved, the Deep Sea Fisheries of oun courtry will be developed more largely.

• Clinical and Experimental Reproductive Medicine
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• 제25권1호
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• pp.59-64
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• 1998

This study was designed to evaluate the influence of pronuclear age on the survival and post-thawing development after cryopreservation of mouse embryos. Freezing and thawing were performed in the different pronuclear stages of mouse embryos after IVF. Embryos were obtained from $F_1$ hybrid mice and classified into 4 groups according to the pronuclear stage (6hr, 9hr, 12hr and 15hr after insemination). Pronuclear ova were slowly cooled in a biological freezer using 1.5M 1,2-propanediol and 0.1M sucrose as cryoprotectant. Thawing was done at room temperature and 1,2-propanediol was removed by multi-step dilutions. Both frozen-thawed embryos and control fresh embryos were cultured in vitro in Ham's F-10 medium supplemented with 4mg/ml BSA. In control group, the development rate after 48hr was 99.3%, and the complete hatching rate after 144hr was 61.3%. In experimental groups, the survival rate after thawing was 95.4% in 6hr, 88.7% in 9hr, 75.2% in 12hr and 62.4% in 15hr after insemination, the development rate after 48hr was 61.1, 77.0, 67.0 and 79.6%, respectively, and the complete hatching rate after 144hr was 25.7, 43.7, 42.2 and 60.0%, respectively. The survival rate in 15hr was significantly lower (p<0.05) compared with other groups. In vitro development rates after 48hr were similar in all groups, but complement hatching rate was significantly lower (p<0.05) in 6hr group. In conclusion, cryopreservation of mouse pronuclear ova with 2 distinct pronuclei (9hr and 12hr groups) showed better results after thawing compared with early (6hr group) or late pronuclear ova just prior to cleavage (15hr group).

• Journal of Acupuncture Research
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• 제17권2호
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• pp.95-117
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• 2000

The purpose of this morphological studies was to investigate the relation between the meridian, acupoints and viscera using neuroanatomical tracers. The common locations of the spinal ganglia, sympathetic chain ganglia, spinal cord and brain projecting to the large intestine meridian were observed following injection of transganglionic tracer, WGA-HRP and transsynaptic neurotropic virus, pseudorabies virus(PRV), Bartha strain(Ba) and PRV-Ba-Gal (Galactosidase)) into the the large intestine(cecum, colon and rectum), ST37 and LI4. After survival times of 96 hours following injection into the thirty rats with WGA-HRP, PRV-Ba and PRV-Ba-Gal. They were perfused, and their spinal ganglia, sympathetic chain ganglia, spinal cord and brain were frozen sectioned($30{\mu}m$). These sections were stained by HRP and X-gal histochemical and PRV immunohistochemical staining method, and observed with a light microscope. The results were as follows : 1. WGA-HRP labeled neurons innervating the large intestine were observed bilaterally within the T13-L4 sympathetic chain ganglia, and T9-11 spinal ganglia. WGA-HRP labeled neurons innervating ST37 were observed within the L3-5 sympathetic chain ganglia, and L2-4 spinal ganglia. WGA-HRP labeled neurons innervating LI4 were observed in the middle cervical ganglion and stellate ganglion, and C5-8 spinal ganglia. 2. In spinal cord, PRV-Ba labeled neurons projecting to the large intestine, ST37 and LI4 were found in thoracic, lumbar and sacral spinal segments. Densely labeled areas of each spinal cord segment were founded in lamina N, V, VII(intermediolateral nucleus), Ⅸ, X and dorsal nucleus. 3. In medulla oblongata, PRV-Ba and PRV-Ba-Gal labeled neurons projecting to the large intestine, ST37 and LI4 were commonly found in the A1 noradrenalin cells/C1 adrenalin cells/caudoventrolateral reticular nucleus, dorsal motor nucleus of vagus nerve, nucleus tractus solitarius, raphe obscurus nucleus, raphe pallidus nucleus, raphe magnus nucleus and gigantocellular nucleus. 4. In pons, PRV-Ba and PRV-Ba-Gal labeled neurons were commonly found in locus coeruleus, Kolliker-Fuse nucieus and A5 cell group. 5. In midbrain, PRV-Ba and PRV-Ba-Gal labeled neurons were commonly found in central gray matter. 6. In diencephalon, PRV-Ba and PRV-Ba-Gal labeled neurons were commonly found in paraventricular hypothalamic nucleus. These results suggest that PRV-Ba and PRV-Ba-Gal labeled common areas projecting to the large intestine may be correlated to that of the large intestine meridian, ST37 and LI4. Especially, These morphological results provide that interrelationship of meridian-acupoints -viscera may be related to the central autonomic pathways.

• 동아시아식생활학회지
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• 제22권2호
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• pp.290-297
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• 2012

쑥을 급여하지 않은 한우육(T0구)과 쑥을 급여한 한우육(T1구)을 $-20^{\circ}C$에서 12개월간 동결한 후 해동하여 일반성분, 무기질, 아미노산 및 지방산 조성을 분석하였다. 한편, 해동 후 냉장에 의한 물리 화학적 및 기호적 특성 변화를 비교하여 쑥의 급여 효과를 검토하였다. 동결 한우육의 일반성분은 T0구와 T1구간의 유의적 차이가 없었다. 무기질에서 Ca, P, K, Mg 및 Zn의 함량은 처리구간의 유의적 차이를 보이지 않았으며, Na은 T0구, Fe는 T1구에서 유의적으로 많게 분석되었다. 아미노산 총량은 처리구간의 유의적 차이가 없었으나, leucine은 T0구가 많은 반면 glycine, cysteine, histidine 및 arginine은 T1구가 유의적으로 많게 분석되었다. 지방산에서 stearic acid는 T0구가 많은 반면, palmitoleic acid, oleic acid 및 불포화지방산 함량은 T1구가 유의적으로 많았다. 경도는 쑥의 급여에 관계없이 해동 후 냉장에 의해 낮아져서 연도가 향상됨을 알 수 있었다. 동결 한우육의 신선도, 지방산패도 및 항산화력의 변화는 T0구보다 T1구가 느리게 나타나서 쑥의 급여에 의한 좋은 효과를 보였다. 가열육의 맛, 다즙성, 연도 및 종합적 기호도는 해동 후 0일째와 3일째 모두 처리구간의 유의적 차이가 없었으나, 향은 쑥을 급여한 T1구가 유의적으로 우수하였다.

• 대한화학회지
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• 제55권4호
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• pp.603-611
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• 2011

Ethoxylacetyl oxime 리간드 [HL, (1) 및 $H_2L^1$, (3)]와 아세트산 구리(II) 일수화물을 반응시켜 팔면체 및 사각평면착물을 각각 얻었다. 이들 착물을 원소분석, IR 및 UV-Vis 스펙트럼, 자기수자율, 전기전도도 및 ESR 스펙트럼으로 조사하였다. DMF에서 이들 착물의 몰전기전도도 측정한 결과 비이온성을 보였다. 실온 및 77 K에서 [$(L)_2Cu(H_2O)_2$], (2) 착물의 ESR 스펙트럼은 배위결합성을 갖는 축대칭($d_{x2-y2}$)의 특성을 보이며, 또한 전형적인 쌍극자 상호작용에 의해 큰 띠나비를 갖는다. 그러나 착물 [$(L^1)Cu$], (4)는 고체상태에서 큰 띠넓임으로 인해 초미세 갈라짐이 관찰되지 않았는데, 이는 구리(II) 결합자리들 사이의 스핀-교환 상호작용에 의한 것으로 확인되었다. 실온에서 도포된 구리(II) 착물의 스펙트럼은 배위된 질소원자로 인해 극초미세 갈라짐을 보였다. 또한 이 물질은 공유 결합성을 갖는 축형태($d_{x2-y2}$)의 특성을 보이고, 구리(II) 이온의 환경은 기본적으로 사각-평면 배열을 갖는다. 77 K의 얼어있는 메탄올에서 [$(L_1)Cu$], (4)의 스펙트럼은 이합체 화합물의 삼중항 상태의 특성을 보이며, 두 구리(II) 이온 중심 사이의 거리를 계산한 결과 4.8 이다.

• Tuberculosis and Respiratory Diseases
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• 제44권3호
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• pp.505-515
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• 1997

배 경 : 폐암환자에서 기관지 침습에 관하여 CT의 정확도를 알아보고, 수술을 시행한 폐암환자에서 CT의 역할을 알아보기 위하여 본 연구를 시행하였다. 대상 및 방법 : 저자들은 폐절제술을 시행한 95명과 엽절제술을 시행한 61명의 수술전 CT를 분석하였다. 엽절제술을 시행 환자중 7명에서는 절제한 기관지 말단에서 암세포가 발견되어 폐절제술을 시행했다. 또한 저자들은 충분한 생검을 한 수술못한 60명의 수술전 CT도 분석하였다. 3mm 이상의 기관지벽의 비후, 불규칙한 기관지벽의 비후 그리고 기관지 직경의 감소를 기관지 침습으로 정하였다. 절제한 기관지 말단의 암세포 발견(20명), 수술한 기관지 말단에서의 폐암재발(6명) 그리고 기관지 생검상 양성(수술 안한군에서 34명)을 기관지 침습으로 생각하였다. 결 과 : 기관지 침습에 관하여 CT는 예민도 (11.5%), 낮은 양성기대치 (38%), 그러나 높은 특이도 (96%), 그리고 비교적 높은 정확도 (84%)를 나타냈으며, 수술 안한 군에서는 높은 예민도(62%), 양성 기대치를 보였다. 결 론 : 시행한 폐암환자에서 CT는 폐암의 기관지 침습에 대하여 예민도와 양성 기대치가 낮았다. 이와 같은 결과로 기관지 침습에 대한 CT의 유용성은 제한적이라 생각되며, 수술전 기관지경에 의한 기관지벽 생검이 필요하리라 생각된다.