• Journal of Korean Society on Water Environment
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• v.26 no.3
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• pp.518-524
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• 2010

Continuous removal of organic matters (CROM) using freshwater bivalve Anodonta woodiana was operated to evaluate the effect of water current on the water quality improvement (clearance) of eutrophic lake. The CROM system comprised three treatment steps such as flow control, treatment and analysis, and operated at the two different currents ($24L\;h^{-1}$ and $48L\;h^{-1}$) with mussels at density of $312.5indiv.\;m^{-2}$ for 12 consecutive days. Water quality including suspended solids (SS) and chlorophyll-a (Chl-a) was daily measured at the same time. Results indicate that although both the system strongly decreased the concentration of SS and chl-a, a slow CROM system was more effective to diminish the SS contents than a fast CROM system; 82% and 66%, respectively (ANOVA, P<0.0001). Clearance rates, based on chl-a, were also significantly higher in a slow system than a fast system (ANOVA, P<0.0001), although the mussel mortality was conversely. In both systems, there showed a remarkable excretion of dissolved inorganic nutrients (i.e. $NH_3-N$ and $PO_4-P$), while a slow CROM system was higher than a fast system, significantly (ANOVA, P<0.0001). Therefore, it may suggest that a slow current CROM system is more suitable to maximize the efficacy of water quality improvement, but further study is needed to diminish the mortality of mussel and to reuse the nutrient released during the operation.

• Korean Journal of Ecology and Environment
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• v.41 no.spc
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• pp.61-67
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• 2008

This study was conducted to evaluate filtering rate (FR) and pseudofeces production (PFP) of a freshwater filter-feeding bivalve, Unio douglasiae, on a toxic cyanobacterium (Microcystis aeruginosa). The experiments were conducted under the various conditions of water temperature $(5{\sim}35^{\circ}C)$, mussel size $(5.6{\sim}13.3cm)$ and food con centrations $(49{\sim}491{\mu}g\;Chl-{\alpha}L^{-1})$. Among the applied temperature, the maximum FR $(0.41L\;gAFDW^{-1}hr^{-1})$ and PFP (0.47mg $gAFDW^{-1}hr^{-1}$) were observed at 15 and $25^{\circ}C$, respectively. Both weight-based FR and PFP were not correlated with the mussel size, and the values lied in a limited range with some degree of variation. Likewise, no significant relations between FR and PFP was observed in the mussel size. The FR values were negatively correlated with food concentration, while PFP showed positive correlation. Among the applied food concentrations, the maximum FR (0.34L $gAFDW^{-1}hr^{-1}$) and PFP (0.06mg $gAFDW^{-1}hr^{-1}$) appeared in $113{\mu}g\;Chl-{\alpha}L^{-1}$ and $491{\mu}g\;Chl-{\alpha}L^{-1}$, respectively. These results indicate that the grazing of Unio douglasiae are affected by various parameters, and it may be applied as an effective biofilter to inhibit Microcystis bloom under appropriate application. However, further studies on the fate of excreted pseudofeces are needed to understand their possibility of stimulating nuisant algal growth.

• Korean Journal of Ichthyology
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• v.26 no.2
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• pp.83-88
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• 2014

An endemic Korean bitterling, Acheilognathus signifer, known as using freshwater mussels for its spawning activity, much prefers Lamprotula leai in Dalcheon stream (Goesan population) and Unio douglasiae sinuolatus in Gadaecheon (Danyang population) stream, Korea. The spawning pattern between two mussels and one bitterling was carried out in two populations from April to June, 2011 and 2013. L. leai in the Goesan population was bigger shell length and have more eggs, larvae or both with A. signifer than those of U. d. sinuolatus in the Danyang population. Also, A. signifer in the Goesan population appeared to have a longer ovipositor and lay ripen eggs more than those of A. signifer in the Danyang population. Glochidium, a larval stage, of some L. leai in the Goesan population was incubated in all the demibranch, whereas for all of U. d. sinuolatus in the Danyang population was found in the outer demibranch only. For L. leai in the Goesan population, the eggs, larvae or both of A. signifer were much more in the non-breeding mussels (giving no glochidium) than the breeding mussels having the glochidium. For U. d. sinuolatus in the Danyang population, in contrast, the non-breeding mussels have fewer eggs, larvae or both less than the breeding one.

• Korean Journal of Environmental Biology
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• v.41 no.3
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• pp.229-246
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• 2023

The bitterling (Cyprinidae, Acheilongnathinae) is a temperate freshwater fish with a unique spawning symbiosis with host mussels. Female bitterlings use their extended ovipositors to lay eggs on the gills of mussels through the mussel's exhalant siphon. In the present study, in April of 2020, we investigated spawning frequencies and patterns of three bitterling fish species in host mussel species in the Nakdong River basin (Hoecheon). During field surveys, a total of four bitterling and three mussel species were found. We observed bitterling's spawning eggs/larvae in the three mussel species: Anodonta arcaeformis(proportion spawned: 45.5%), Corbicula fluminea(12.1%), and Nodularia douglasiae (45.2%). The number of bitterlings' eggs/larvae per mussel ranged from 1 to 58. Using our developed genetic markers, we identified the eggs/larvae of each bitterling species in each mussel species (except for A. macropterus): A. arcaeformis (spawned by Acheilognathus yamatsutae), C. fluminea (A. yamatsutae and Tanakia latimarginata), and N. douglasiae (A. yamatsutae, Rhodeus uyekii, and T. latimarginata). Approximately 57.6% of N. douglasiae mussel individuals had eggs/larvae of more than one bitterling species, suggesting that interspecific competition for occupying spawning grounds is intense. This is the first report on bitterling's spawning events in the Asian clam C. fluminea from Korea; however, it should be ascertained whether bitterling's embryo undergoes successful development inside the small mussel and leaves as a free-swimming juvenile. In addition, the importance of its conservation as a new host mussel species for bitterling fishes needs to be studied further.

• Korean Journal of Ecology and Environment
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• v.47 no.4
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• pp.312-318
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• 2014

This study was conducted to establish models of filtrating rate and production of feces of a native freshwater bivalve, Anodonta woodiana, on removal organic matters in eutrophic waters. Among the applied shell size (4.3~15.5 cm), the filtrating rate and production of feces of Anodonta woodiana was $0.08{\sim}0.86L\;g^{-1}\;h^{-1}$ (average $0.24L\;g^{-1}\;h^{-1}$), $0.00{\sim}11.10mg\;g^{-1}\;h^{-1}$ (average $0.94mg\;g^{-1}\;h^{-1}$), respectively. In two different water current (high $48Lh^{-1}$, low $24Lh^{-1}$), the filtrating rate of Chl-a was $0.02{\sim}0.10L\;g^{-1}\;d^{-1}$ (average $0.05L\;g^{-1}\;d^{-1}$), $0.02{\sim}0.11L\;g^{-1}\;d^{-1}$ (average $0.07L\;g^{-1}\;d^{-1}$) and the removal rate was 65.4%, 82.1%, respectively. Response surface methodology, with a central composite design comprising 3 levels and 2 variables, was used to identify the optimal removal condition of shell length, water current and filtrating rate or feces production by bivalves. The optimum removal conditions were found that had optimized $6.21L\;mussel^{-1}\;d^{-1}$ at shell length 14.3~15.6 and water current $22{\sim}30Lh^{-1}$. The optimal conditions of production of feces ($4.2mg\;g^{-1}\;d^{-1}$) by freshwater mussels were shell length 14.3~16.3 cm and water current $36{\sim}44Lh^{-1}$.

• Korean Journal of Ecology and Environment
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• v.35 no.2 s.98
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• pp.92-102
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• 2002

This study was conducted to compare filtering abilities of three species of freshwater mussels (Cobicula fluminea, Corbicula leana and Unio douglasiae) and to evaluate their filter feeding effects on water quality change in experimental enclosure systems. Mussel feeding in both laboratory and enclosure resulted in decrease of particulate material, such as chlorophyll, total P, SS. In the treatment with 600 individuals of mussels, chllorophyll concentration and net primary productivity decreased from $87.3{\pm}4.5\;{\mu}g/L$ and $106.3{\pm}8.8\;{\mu}gC\;L^{-1}\;hr^{-1}$ to nearly the same level as the mussel-free enclosure ($25.0{\pm}0.5\;{\mu}g/L$ and $15.6{\pm}13.3\;{\mu}gC\;L^{-1}\;hr^{-1}$, respectively)(P< 0.05, n = 6, ANOVA). In concert with the decrease of chlorophyll concentration, not only was the transparency enhanced from 0.48 m to 1.2m but also the suspended solids and total phosphorus decreased from $22.0{\pm}1.0\;mg/L$ to $7.5{\pm}0.5\;mg/L$ and $133{\pm}0.8\;{\mu}g/L$ to $70{\pm}0.0\;{\mu}g/L$, respectively (P<0.001, $r^2$>0.71, n = 11). Although slight decrease of SRP concentration and the increase of inorganic nitrogen ($NH_3-N$ and $NO_2-N$) were observed in the mussel addition enclosure, there was no statistical difference between two enclosures. Based on the filtering rate on phytoplankton and nutrient release rate in forms of feces and pseudofeces, Corbicula leana appeared to be the most efficient filter-feeder among three mussel species. These results inidicate that Cobicula play an important role in controlling particulate sestons and thus it could be applied as a biocontroler for the water quality management in lakes and reservoirs with algal blooms.

• Korean Journal of Ecology and Environment
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• v.48 no.2
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• pp.108-114
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• 2015

Single - and combined effects of a domestic freshwater bivalve Unio douglasiae (7.6~8.6 cm in shell length) and zooplankton Daphnia magna (1~2 mm in body size) were examined to understand whether they inhibit the growth of harmful cyanobacterial bloom (i.e. Microcystis aeruginosa) in a eutrophic lake. The experiments were triplicated with twelve glass aquaria (40 L in volume); three aquaria without mussel and zooplankton, served as a control, three zooplankton aquaria (Z, density=40 indiv. $L^{-1}$), three mussel aquaria (M, density=0.5 indiv. $L^{-1}$), and three mussel plus zooplankton aquarium (ZM, density=40 indiv.Z $L^{-1}$ plus 0.5 indiv.M/L), respectively. Algal growth inhibition (%) calculated as a difference in the concentration of chlorophyll-a (Chl-a) before and after treatment. Chl-a in all aquaria decreased with the time, while a greatest algal inhibition was seen in the ZM aquaria. After 24 hrs of incubation, Chl-a concentration at the mid-depth (ca. 15 cm) in ZM aquaria reduced by 90.8% of the control, while 63.2% and 79.8% in Z and M aquaria, respectively. Interestingly, during the same period, the surface Chl-a was diminished by 51.9% and 65.4% relative to the control in Z and ZM aquaria, while 27.4% of initial concentration decreased in M aquarium, respectively. These results suggest that 1) this domestic freshwater filter-feeding bivalve plays a significant role in the control of cyanobacterial bloom (M. aeruginosa), and 2) the combination with zooplankton and mussel has a synergistic effect to diminish them, compared to the single treatment of zooplankton and mussel.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.1
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• pp.43-47
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• 2001

Electron spin resonance (ESR) spectroscopy was used to investigate the effect of irradiation dose on the ESR signal intensity and to identify the stability of radicals after 9 weeks of storage in order to detect irradiated shellfishes. The irradiated shellfishes (short-necked clam, purplish washington clam, freshwater clam, jackknifed clam, scallop and hard-shell mussel) presented an asymmetric absorption in shape at $g_{1}$=2.002~2.003 and $g_{2}$=1.998. The strength of the ESR signal increased linearly with the applied doses (1~7 kGy). A highly positive correlation coefficients ($R^{2}$=0.9136~0.9896) were obtained between the irradiation dose and corresponding ESR signal intensity. The intensity of the signals after irradiation was stable even after 9 weeks of storage at 5$\pm$1$^{\circ}C$.

• Journal of the Korean Society for Marine Environment & Energy
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• v.17 no.2
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• pp.131-145
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• 2014

The new passive sampler called "artificial mussel (AM)" offers a potential device to study the spatiotemporal changes of metal concentrations in different marine environment worldwide. The purpose of this study is to characterize metal (Cd, Cr, Cu, Zn, Pb) accumulation on the AM and transplanted mussel (Mytilus edulis) at 5 sites of Lake Shihwa. Both the AMs and mussels showed increasing concentrations of all five metals during the 12 weeks exposure period. Higher concentrations of Zn were showed in both the AMs and Mytilus edulis relative to other metals. The AMs accumulated higher concentrations of Cd, Cr and Zn, but they presented lower levels of Cu and Pb than Mytilus edulis. The correlations for Cd, Cu and Pb were statistically significant between the AMs and Mytilus edulis, indicating that the accumulation patterns for those metals were similar. However, no similarities for Cr and Zn were observed between two monitoring devices across all of the sites in Shihwa Lake. According to relationship for metal concentrations between dissolve phase in seawater and both the AMs and Mytilus edulis, the AMs for Cd, Cu and Zn represent more metal contamination than Mytilus edulis. Our results indicated that the AMs give a better resolution to reveal the spatial differences in dissolved metal concentration. This study suggests that the AMs can provide a time-integrated estimate of metal pollution in marine environments as well as freshwater environments of Korea.

• The Korean Journal of Malacology
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• v.25 no.2
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• pp.153-160
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• 2009

Pisidium (Neopisidium) coreanum is a freshwater snail and lives in spring water near mountain areas. Interestingly, this snail has been traditionally regarded as medicinal food, and thus has been used as folk remedies for healing broken bones. Recently, alpha classification on Pisidium (Neopisidium) coreanum through redescription has been conducted. However, not much attention has been made in beta classification. In this study, we performed the beta classification based on metallothionein (MT) genes found from various organisms. To this end, the complete cDNA sequences were obtained from the Expressed Sequence Tag (EST) sequencing project of Pisidium (Neopisidium) coreanum. The coding region (315 bp) encoded an amino acid sequence of 105 residues. The combined results from BLAST analyses, multiple sequence alignment and molecular phylogenetic study of Pc-MT gene indicate that Pisidium (Neopisidium) coreanum has similarity to freshwater bivalve such as Dreissena polymorpha (zebra mussel), Unio tumidus (swollen river mussel) and Crassostrea ariakensis (suminoe oyster).