• 콘크리트학회논문집
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• 제11권4호
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• pp.117-127
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• 1999

In concrete incorporating high volume ground granulated blast-furnace slag that has frozen at early age, to evaluated the results of resistance to freezing and thawing is very difficult because the hydration of the concrete increases over the duration of rapid freezing and thawing test. Hence, the dynamic modulus of elasticity of specimens after freezing and thawing will be favorable results unless the hydration effect is taken into consideration. In this study, a method of evaluating to the resistance to freezing and thawing of concrete subjected freezing at early age, in which the effect of hydration is modified for its increase during rapid freezing and thawing test, is investigated.

• 한국농림기상학회지
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• 제16권1호
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• pp.51-58
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• 2014

배의 휴면기간 중 저온 유지 시간 및 온도에 따른 내동성을 비교하고자 감천배, 만풍배, 신고, 원황, 추황배, 풍수, 한아름, 행수, 화산, 황금배 등의 1년생 가지를 각각 $-21^{\circ}C$, $-24^{\circ}C$, $-27^{\circ}C$, $-30^{\circ}C$, $-33^{\circ}C$의 온도로 6, 9, 12시간 동안 처리한 후 꽃눈 발아율, 전해질 누출률, TTC검정을 하였다. 저온처리 시간에 따른 꽃눈발아율은 $-21^{\circ}C$에서 6시간일 경우 품종간 거의 차이가 없었으나 온도가 낮아지고 지속시간이 길어질수록 낮아졌다. 전해질 누출률은 $-30^{\circ}C$ 이하에서는 저온지속시간에 관계없이 대부분의 품종이 30%이상의 높은 전해질 누출률을 보여 온도가 낮아질수록 저온지속 시간이 길수록 전해질 누출률이 높아졌다. 저온에서 높은 발아율을 보였던 만풍배, 신고, 추황배는 $-30^{\circ}C$ 9시간에서 각각 26.1%, 14.7%, 28.3%로 다른 품종보다 낮은 전해질 누출률을 보였다. TTC 검정을 통한 흡광률은 $-21^{\circ}C$에서 6시간일 경우 66.0~96.5%, 9시간에는 49.4~91.9%, 12시간에는 37.3~89.4%로 저온유지 시간이 길어질수록 흡광률이 낮아졌다. 배의 품종별 내동성 확인 결과 꽃눈발아율은 전해질누출률 또는 TTC 검정을 통한 흡광률과의 높은 상관관계를 보였으며 배의 외재휴면 기간 중 내동성은 저온 유지시간 및 온도에 따라 다르며 품종별 저온 피해 한계온도가 다름을 확인할 수 있었다.

• 한국도로학회논문집
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• 제7권2호
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• pp.87-95
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• 2005

동절기에 대기온도가 영하로 내려감에 따라 포장하부층의 동결 및 응해가 반복되어 포장체의 구조적 성능이 저하된다. 이를 방지하기 위하여 동상에 대한 많은 연구가 수행되었으며, 그 결과, 우리나라 전역의 동결지수선도가 작성되어 동상방지층 설계 등의 도로건설에 이용되고 있다. 하지만, 포장층의 두께와 재료, 그리고 지형에 따른 동결심도의 변화에 대한 조사가 체계적으로 이루어지지 않아 현행 동상방지층 설계의 유효성에 대한 평가가 시급한 실정이다. 본 논문에서는 한국도로공사 시험도로에서 측정되는 대기온도 자료와 콘크리트 및 아스팔트 포장의 깊이별 온도 및 함수량 자료를 이용하여, 시험도로의 동결지수 및 동결가능 깊이를 조사하였다. 또한, 조사된 결과를 기존의 동결심도 추정식에 의한 결과와 비교하였다. 본 연구는 시험도로에서 측정된 동결 관련자료에 대한 초기연구로서 , 장기적인 자료의 축적과 분석을 통하여 보다 신뢰성 있는 결과가 도출될 수 있을 것으로 판단된다.

• Geomechanics and Engineering
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• 제31권6호
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• pp. 583-597
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• 2022

Frost heave can cause uneven ground uplift that may damage geo-infrastructure. To assist damage-prevention strategies, standard frost heave testing methods and frost susceptibility criteria have been established and used in various countries. ASTM International standard testing method is potentially the most useful standard, as abundant experimental data have been acquired through its use. ASTM International provides detailed recommendations, but the method is expensive and laborious because of the complex testing procedure requiring a freezing chamber. A simple frost heave testing method using a temperature-controllable cell has been proposed to overcome these difficulties, but it has not yet been established whether a temperature-controllable cell can adequately replace the ASTM International recommended apparatus. This paper reviews the applicability of the ASTM International testing method using the temperature-controllable cell. Freezing tests are compared using various soil mixtures with and without delivering blow to depress the freezing point (as recommended by ASTM International), and it is established that delivering blow does not affect heave rate, which is the key parameter in successful characterization of frost susceptibility. As the freezing temperature decreases, the duration of supercooling of pore water shortens or is eliminated; i.e., thermal shock with a sufficiently low freezing temperature can minimize or possibly eliminate supercooling.

• Geomechanics and Engineering
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• 제13권5호
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• pp.793-807
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• 2017

Regularities of temperature variation were determined in points of subgrade of the highway. Measurement of temperature was performed by special sensors, based on the effect of thermal resistance. Regular measurements of temperature were performed for two sections of the highway with asphalt concrete and cement concrete pavements for continuous period from November 2010 to March 2016. Multi-year experimental data, which we obtained, allowed establishing of peculiarities for temperature variation in points of subgrade in time and temperature distribution in the depth for annual cycle. Characteristics were determined for winter period-depth, duration and freezing rate, duration and defreezing rate for pavement and subgrade of the highway.

• 한국지반공학회논문집
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• 제29권1호
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• pp.81-92
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• 2013

본 논문에서는 계절적 환경변화로 인한 동결-융해 반복과정에 노출되는 옹벽의 시간 의존적 거동에 대한 수치 해석 연구 내용을 다루었다. 이를 위해 먼저 동결-융해 반복과정에 노출되는 옹벽에 대한 열-수리-응력 연계해석 기반의 모델링 기법을 정립하였으며 이러한 모델링 기법을 토대로 다양한 온도변화 조건에 대한 매개변수 연구를 수행하였다. 그 결과 다양한 온도변화 특성 중 옹벽의 거동에 미치는 주된 영향인자는 계절적 평균동결온도와 반복회수인 것으로 나타났다. 반면 동결온도의 지속시간에 대한 영향을 검토한 결과 벽체변위 및 수평토압에 큰 영향을 미치지 않는 것으로 검토되었다. 한편, 벽체에 작용하는 수평토압은 동결-융해 작용에 의한 영향을 거의 받지 않는 것으로 검토되어 결국 동결-융해로 인한 벽체 변위 증가 현상은 토압 증가에 그 원인을 찾기 보다는 동결-융해 현상으로 인한 재료적 열화 현상에 그 원인을 찾아야 할 것으로 검토되었다.

• 한국수산과학회지
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• 제6권3_4호
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• pp.101-111
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• 1973

생 전복을 액체질소동결, 송풍동결, 반송풍동결, 정지공기동결의 네가지 동결방법으로 동결하여 $-20^{\circ}C$에서 3개월간 저장하여 동결방법에 의한 전복근육의 물성적 변화, 단백질의 변성 등을 검토하여 다음과 같은 결과를 얻었다. 1) 생전복의 사후경직은 다른 어류보다 빨랐다. 2) 압측시험치, 전단시험치는 액체질소동결을 제외하고는 동결방법에 의한 차는 없었고, 저장기간이 길어짐에 따라 약간 감소하였다. 3) 염가용성질소는 동결저장기간중 용출량이 완만하게 감소하였다. 4) 유리수의 유출량은 패주와 다리 사이에는 차가 있었고, 저장기간중 패주는 유리수가 증가하는 경향이었다. 5) Texture의 Panel test 결과 저장기간을 통하여 Texture가 감소하는 경향이었으며, Texture의 변화는 유리수의 변화(P<0.01), 단백질의 변성(P<0.05), 압축시험치의 변화(P<0.05)와 유의의 상관관계가 있었다. 본 논문을 지도하여 주신 동경수산대학 교수 전중 화부 박사 그리고 논문의 작성에 조언해 주신 부산 수산대학 식품공학과 이응호 박사님께 감사의 뜻을 표한다.

• 한국작물학회지
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• 제48권5호
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• pp.407-412
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• 2003

In order to measure an antifreezing tolerance, antifreeze proteins accumulated upon cold acclimation in apoplast were analyzed. As Dongborilho were cold-acclimated for 3 to 74 days there was an abrupt increase in apoplastic proteins up to 30 days and then decrease to the similar levels. Among the known antifreeze proteins, CLP produced in E. coli. and TLP purified from apoplast were used to generate antisera that allow to measure and localize the proteins in leaves of barley. The CLP of 27.7 kDa and TLPs of 6, 26, 27, 30, and 31 kDa were increased in their amounts in apoplast as cold treatment being longer. There was a correlation among the amounts of those proteins accumulated in apoplast and freezing tolerance as shown in field and ion leakage tests for five cultivars. The deposit of CLP was localized in the marginal area and the area adjacent to leaf vescular bundle cells in an increasing manner according to duration of cold acclimation but no variation was observed in terms of it's distribution. Based on the close correlation between levels of antifreeze proteins and degrees of freezing tolerance, the immunological methods was to develop to estimate a freezing tolerance in barley.

• Clinical and Experimental Reproductive Medicine
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• 제25권3호
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• pp.261-268
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• 1998

The present study was to assess the effect of ultrarapid freezing on the development of 1-cell mouse zygote using cryoprotectants, DMSO (dimethyl sulfoxide) or PROH (1,2-propanediol). We investigated the effect of the type and concentration of cryoprotectant, and of the temperature and time of prefreezing equilibration on their capacity to develop to the blastocyst stage in vitro. The concenration, the equilibration temperature, and the exposure time seemed to serve as an important factor in ultrarapid freezing of 1-cell mouse zygotes. In addition to the exposure time and the concentration of cryoprotectant appeared to playa key role in the development of the embryo. In general, the development of the embryo was more effective at $3^{\circ}C$ than $23^{\circ}C$ and 4.5 M than 3 M for 3 to 5 minutes. At $23^{\circ}C$ the development of the embryo was stimulated by DMSO while at $3^{\circ}C$ it was stimulated by PROH. Thus it has been suggested that there exists a correlation between the concentration of cryoprotectants and exposure time in the development of the embryo. In conclusion, we found that for ultrarapid freezing of mouse 1-cell embryos in DMSO, or PROH-based solution, viability shown optimum depending on the cryoprotectant, the concentration of the cryoprotectant and on the temperature and the duration of equilibration.

• 한국수정란이식학회지
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• 제11권3호
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• pp.271-281
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• 1996

The effect of superovulation (PMSG, FSH) on the ovarian response of matured cows were tested. The survival rates of bovine embryos and ovarian oocytes frozen by slow, rapid freezing and vitrification were investigated. A total of 15 heads of cow were devided into 3 groups by injection dose of GTH (PSMG, FSH). Each group was superrovulated with injections of 2, 500, 3, OOOJU PSMG and 40mg FSH followed by injection of 30mg PGF2a. Embryos were non-surgically recovered from superovulated cows 6~7days after estrus. The recovered embryos were frozen in 10% glycerol + 10% sucrose by slow and rapid freezing. Ovarian oncytes were frozen in 20% g]ycerol+l0% ethylene glycerol + 30% Ficol + 10% sucrose by vitrification and the survival of frozen embryos and ovarian oncytes were judged by FDA-test. The results are summarized as follows; 1. Estrus after the injection of 2500, 3000 I.U. PMSG and 4Omg FSH were 32.8, 35.0 and 43.4 and the duration of estrus were 18.6, 18.8 and 22.4 hours respectively. 2. The average sizes of the left ovaries were 5.4cm (2, 500 IU PMSG), 5.1cm (3, OOOIU PMSG) and 6.4cm (FSH), and the right were 6.2cm (2, 5001U PMSG), 5.7cm (3, OOOIU PMSG) and 7.&m (FSH) respectively. There were significant differences in the right overies among treatments (P<0.05). 3. The average number of ovarian follicles in the left ovaries were 4.8 (2, 500 IU PMSG), 5.2(3, 000 IU PMSG) and 7.8 (FSH) respectively. There were significant difference in the right ovaries among treatments (P<0.05). 4. In the average numbers of ovulation points in the left ovaries were 3.0 (2, 5001U PMSG), 3.2 (3, OOOIU PMSG) and 4.4 (FSH) respectively, and the right were 7.2 (2, 5001U PMSG), 7.8(3, 000IU PMSG) and 11.4 (FSH). There were significant differences in the right ovaries among treatments (P<0.05). 5. The numbers of the recovered embryos were 20 (2, 5001U PMSG), 19 (3, 000 IU PMSG) and 21 (FSH) respectively, and oncytes and degenerted oncytes were 6.5 and 11.0 Estrus periods of post parturation were 52.4days (2, 5001U PMSG), 69.8days (3, OOOIU PMSG) and 62.4days (FSH) respectively. 6. The FDA score of cow morulae frozen by slow freezing, sernirapid frezing and vitrified freezing was higher in slow (3.1) and vitrified freezing (3.0) than that in semirapid freezing (1.28). The FDA-scores of cow, pig and rabbit ovarian oocytes frozen in 20% glycerol + 10% ethylene glycol + 30% Ficoll + 10% sucrose by vitrification were higher in cows (3.3) than both in pigs (2.6) and rabbits (2.3).