• Journal of Korean Dental Science
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• v.8 no.2
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• pp.57-64
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• 2015

Purpose: This study was conducted to determine cell viability and differentiation capability of human periodontal ligament (PDL) cells and to elucidate the effects of cryopreservation on the activity of human third molar PDL cells by comparing PDL cells with and without cryopreservation. Materials and Methods: Human PDL fibroblasts obtained from immature third molars were cultured and divided into two groups. The experimental group was cryopreserved with a slow freezing rate of $0.5^{\circ}C/min$ from $4^{\circ}C$ to $-35^{\circ}C$ followed by plunging in liquid nitrogen at $-196^{\circ}C$ and cultured after fast thawing. The control group was cultured without cryopreservation. Cell viability, growth capacity and morphology were evaluated in both groups. Bivariate statistics were used to compare 2 groups and linear mixed model analysis was used to investigate the growth trends difference over time. Result: Cell viability and growth capacity were not significantly different between the 2 groups (P>0.05). Cultured cell of both groups showed fibroblast-like in appearance, and there were no significant differences in morphology between 2 groups. The mixed model analysis revealed no significant difference of growth capacity between 2 groups over time (${\beta}=-0.0009$; P=0.138). Conclusion: This study demonstrates that cryopreservation under control does not affect the biological properties of PDL cells, supporting the feasibility of autotransplantation of cryopreserved impacted third molars.

• The Journal of Korean Society for School & Community Health Education
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• v.8 no.2
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• pp.79-95
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• 2007

A study was conducted in order to develope HACCP model in school lunch in Korea. Results: 1. Of 22 menus 4(18%) were non heating processes (#1), 2(9%) were food handling by using hands (#2), and 16(73%) were heating processes (#3). Of 279 menus 36(12.9%) belong to process #1, 8(2.9%) to process #2, and 235(84%) to process #3. 2. The critical control points for process #1 were contamination by hands of food handlers, and unsanitary food preparation habits of food handlers. Those for process #2 were improper heating temperature, contaminations by food handlers' hands, and unsanitary food handling habits, and cross contamination by unclean utensils and equipment. 3. Management criteria for the CCPs were conditions of food storage, refrigeration, freezing, food cooking temperature, personal hygiene, washing and sanitization of utensils and equipment. 4. Monitoring criteria for CCPs were observation, temperature checking, inspection of utensils and equipment, and practice of good personal hygiene. 5. Corrective actions were refusal of unsafe products, correction of improper temperature, proper cleaning and sanitization, and proper reheating time and temperature.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.16 no.3
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• pp.219-228
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• 2003

Numerical and experimental studies were conducted on the temperature distribution of a buried steel pipe and surrounding granite frozen soils in the closed system. The relationship between unfrozen water content and temperatures was analysed by laboratory test. The thermal conductivity measurements were made to compare the results with a formula presented by Lachenbruch. A steel container model that consists of a freezing chamber and a buried circular steel pipe was built for the laboratory temperature measurements. The time temperature records were measured experimentally, and those records were compared with numerical results obtained from FEM analysis in order to verify the feasibility. The latent heat effect on the granite frozen soils in the numerical study was considered.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2017.05a
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• pp.679-681
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• 2017

Numerical analysis was carried out by applying the concept of E-D(expansion-deflection) nozzle to dual bell nozzle. We used the CEA code to calculate the chemical composition of the nozzle and to analyze the freezing flow of 8 species. The turbulence model was chosen as the $k-{\omega}$ SST. We applied the concept of E-D nozzle to the dual bell nozzle and performed the calculated transition altitude and performance. As a result of the interpretation, the application of the E-D nozzle concept led to the formation of over-expansion conditions, which resulted in an increase in the transition altitude.

• Journal of Korea Multimedia Society
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• v.24 no.7
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• pp.849-859
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• 2021

Prostate cancer (PCa) is a fatal disease that occurs in men. In general, PCa cells are found in the prostate gland. Early diagnosis is the key to prevent the spreading of cancers to other parts of the body. In this case, deep learning-based systems can detect and distinguish histological patterns in microscopy images. The histological grades used for the analysis were benign, grade 3, grade 4, and grade 5. In this study, we attempt to use transfer learning and fine-tuning methods as well as different model architectures to develop and compare the models. We implemented MobileNet, ResNet50, and DenseNet121 models and used three different strategies of freezing layers techniques of fine-tuning, to get various pre-trained weights to improve accuracy. Finally, transfer learning using MobileNet with the half-layer frozen showed the best results among the nine models, and 90% accuracy was obtained on the test data set.

• Journal of Korean Society of Transportation
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• v.18 no.5
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• pp.7-16
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• 2000

This paper is focused on verifying time-space repetition of the highway accident and finding the their causes and deterrents. We classify all months into several seasonal groups, develop the model for each seasonal group and analyze the results of these models for Joong-bu highway. The existence of seasonal effect is verified by the analysis or self-organizing map and the accident indices. Agglomerative hierarchical cluster analysis which is used to decide the seasonal groups in accordance with accident patterns, winter group, spring-fall group. and summer group. The accident features of winter group are that the accident rate is high but the severity rate is low. while those of summer group are that the accident rate is low but the severity rate is high. Also, the regression model which is developed to identify the accident Pattern or each seasonal group represents that the season-related factors, such as the amount of rainfall, the amount of snowfall, days of rainfall, days of snowfall etc. are strongly related to the accident pattern of evert seasonal group and among these factors the traffic volume, amount of rainfall. the amount of snowfall and days of freezing importantly affect the local accident Pattern. So, seasonal effect should be considered to the identification of high-risk road section. the development of descriptive and Predictive accident model, the resource allocation model of accident in order to make safety management plan efficient.

• The Journal of the Korea Contents Association
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• v.16 no.4
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• pp.114-122
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• 2016

Concrete is cost-benefit and high-durable construction material, however durability problem can be caused due to steel corrosion under chloride attack. Recently deicing salt has been widely spread in snowing season, which accelerates micro-cracks and scaling in surface concrete and the melted deicing salt causes corrosion in embedded steel. The previous governing equation of Fick's 2nd Law cannot evaluate the deteriorated surface concrete so that another technique is needed for the surface effect. This paper presents chloride penetration analysis technique for concrete subjected to deicing salt utilizing multi-layer diffusion model and time-dependent diffusion behavior. For the work, field investigation results of concrete pavement exposed deicing salt for 18 years are adopted. Through reverse analysis, deteriorated depth and increased diffusion coefficient in the depth are evaluated, which shows 12.5~15.0mm of deteriorated depth and increased diffusion coefficient by 2.0 times. The proposed technique can be effectively applied to concrete with two different diffusion coefficients considering enhanced or deteriorated surface conditions.

• Korean Journal of Agricultural and Forest Meteorology
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• v.6 no.4
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• pp.235-241
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• 2004

There is a growing concern about the possible increase in inter-annual variation of minimum temperature during the winter season in Korea. This view is strengthened by frequently reported freezing injury to dormant fruit trees, while warmer winters have prevailed recently. The January minimum temperature record at fourteen weather stations was analyzed for 1951-2000. The results showed no evidence of increasing standard deviation at 3 locations between 1951-1980 and 1971-2000, while the remaining 11 stations showed a trend of decreasing standard deviation for the two periods. An empirical model explaining the spatial variation of the standard deviation was derived by regression analysis of 56 stations' data for 1971-2000. Daily minimum temperature and the site elevation may account for 68% of the observed variations. We applied this model to restore the average standard deviation of the January minimum temperature for 1971-2000, and the result was used to produce gridded minimum temperature data for the recurrence interval of 10 and 30 years at 250m resolution. A digital form of the plant hardiness zone map may be developed from this product for site-specific selection of adapted plant species.

• Journal of the Korean earth science society
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• v.36 no.6
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• pp.501-511
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• 2015

This study suggests a method of alleviating global warming by the increase of the Earth surface albedo through Artificial Sea ice Increasing (ASI) over the Available Freezing Areas (AFA). The method is developed based on the fact that the large sea surface area in or near the Arctic and the Antarctic has no ice even though both water and air temperatures are below zero and the artificial sea ice generation is thus available. The mean energy of $0.85Wm^{-2}$, which was suspected of adding to the earth by the global warming effect was calculated to offset at once when the sea ice area about $4.09{\times}10^6km^2$ was additionally increased. In addition, three techniques for producing ice plates on the sea surface (using ships, installation apparatus, and floating matter such as Green Cell Foam) for ASI were proposed. According to the result of simple analysis using the energy balance model, when ASI was maximally operated only for 3 months (September, October, and November) over AFA, it is expected that the annual mean temperature of earth surface would be decreased about $0.11^{\circ}C$ in the following year. On the other hand, in case of generating the artificial sea ice in all four seasons, a risk of triggering snowball earth was detected.

• Journal of the Korean Society for Marine Environment & Energy
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• v.14 no.4
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• pp.301-306
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• 2011

The paper presents an analysis of the heating cycle and discusses a desalination cycle that uses lowtemperature seawater. The basic heating cycle model is the heat pump cycle, and seawater desalination is usually performed by the indirect freezing desalination method. The low temperature of the seawater (below $5^{\circ}C$) acts as the heat source of the evaporator. R-134a, R-1234yf, R-600a are used as working fluids. In the 2-stage compression cycle, the compressor's work decreased by about 15.6% from that in the 1-stage compression cycle. Further, the COP in the 2-stage cycle was 17.6% higher than that in the 1-stage cycle. In the indirect desalination cycle, the energy per unit fresh water productivity in the 2-stage cycle was 19.8% lower than that in the 1-stage cycle.