• Proceedings of the Korean Society of Applied Pharmacology
• /
• 2000.04a
• /
• pp.6-7
• /
• 2000

Recently, free radicals such as active oxygen species, nitric oxide, etc are believed to be one of the key substances in physiological and pathological, toxicological phenomena, and oxidative damages, and all organism have defencing system against such as free radicals. Formation and extinction of free radicals may be regulated through bio-redox system, in which various enzymes and compounds should be involved in very complicated manner. Thus, direct and non-invasive measurement of in vivo free radical reactions with living animals must be essential to understand the role of free radicals in pathophysiological phenomena. Electron spin resonance spectroscopy (ESR) is very selective and sensitive technique to detect free radicals, but a conventional ESR spectrometer has large detect in application to living animals, since high frequent microwave is absorbed with water, resulting in generation of high fever in living body. In order to estimate in vivo free radical reactions in living whole animals, we develop in vivo ESR-CT technique using nitroxide radicals as spin probes. Nitroxide radicals and their reduced forms, hydroxylamines, are known to interact with various redox systems. We found that! ! the signal decay due to reduction of nitroxyl radicals is influenced by aging, inspired oxygen concentration, ischemia-referfusion injury, radiation, etc. In the present paper, I will introduce in vivo ESR technique and my laboratory recent results concerning non-invasive evaluation of free radical reactions in living mice.

• Biomedical Science Letters
• /
• v.17 no.1
• /
• pp.7-12
• /
• 2011

Free-living amoebae ingest several kinds of bacteria. In other words, the bacteria can survive within free-living amoeba. To determine how Escherichia coli K1 isolate causing neonatal encephalitis and non-pathogenic K12 interact with free-living amoebae, e.g., Acanthamoeba castellanii (T1), A. astronyxis (T7), Naegleria fowleri, association, invasion and survival assays were performed. To understand pathogenicity of free-living amoebae, in vitro cytotoxicity assay were performed using murine macrophages. T1 destroyed macrophages about 64% but T7 did very few target cells. On the other hand, N. fowleri which needed other growth conditions rather than Acanthamoeba destroyed more than T1 as shown by lactate dehydrogenase (LDH) release assay. In association assays for E. coli binding to amoebae, the T7 exhibited significantly higher association with E. coli, compared with the T1 isolates (P<0.01). Interestingly, N. fowleri exhibited similar percentages of association as T1. Once E. coli bacteria attach or associate with free-living amoeba, they can penetrate into the amoebae. In invasion assays, the K1 (0.67%) within T1 was observed compared with K12 (0%). E. coli K1 and K12 exhibited high association with N. fowleri and bacterial CFU. To determine the fate of E. coli in long-term survival within free-living amoebae, intracellular survival assays were performed by incubating E. coli with free-living amoebae in PBS for 24 h. Intracellular E. coli K1 within T1 (2.5%) and T7 (1.8%) were recovered and grown, while K12 were not found. N. fowleri was not invaded and here it was not recovered.

• Journal of Nutrition and Health
• /
• v.35 no.3
• /
• pp.332-351
• /
• 2002

The purpose of this study was to investigate effect of living condition and aging on food intake in Korea. 98 free-meal receivers and 81 members in well-being center for the aged were asked about their food frequency and food consumption and their socioeconomic status by a questionnaire. All data were analyzed by SPSS (Statistical Packages for the Social Sciences) 10.0 program. There were statistical differences of living condition, marital status, former job and teeth status between free-meal receivers and people with stable lives. Moreover, all age groups of free-meal receivers had lower income, living expenses, educational period and frequency of physical activity than those of people with stable lives. But smoking and drinking was much higher in free-meal receivers. Arthritis was the most prevalent disease in both groups. And free-meal receivers had higher blood pressure and lower Body Mass Index than people with stable lives. Food consumption of free-meal receivers was definitely lower than that of people with stable lives in terms of food frequency and standard amount of food eaten. But noodles, bean sprouts and eggs were very important foods for free-meal receives. Food consumption of the aged was affected by age, educational period, income, blood pressure, duration after retirement, frequency of physical activity and Body Mass Index. Therefore, as various socioeconomic status affects on food intakes of the aged, more effective and practical nutritional programs which consider the receivers'socioeconomic status are needed for the nutritionally at-risk groups like the aging free-meal receivers.

• Journal of The Korea Institute of Healthcare Architecture
• /
• v.15 no.1
• /
• pp.33-40
• /
• 2009

This study aims to plan connecting living facilities considering on the Barrier Free Walking of the disabled and the elderly in the residential area. We had found several physical problems on the pedestrian's way that becomes the major factor of disturbing the barrier free movement for using living facilities in the local area by investigations and enquetes. We had suggested the arrangement method for these physical problems on the pedestrian's way and building the ideal area for living facilities by the disposition stages with the result of investigations and enquetes. After these process, we had planned connecting living facilities considering on the Barrier Free Walking of the disabled and the elderly in the residential area.

• Journal of the Korean Society of Food Culture
• /
• v.17 no.3
• /
• pp.275-288
• /
• 2002

The purpose of this study was to investigate the cognitive differences among the aged who have different living conditions. 91 free-meal receivers and 86 people with stable lives were asked about their living conditions and cognitive function by using K-MMSE. All data were analyzed by SPSS 10.0 package. Free meal receivers had poorer socioeconomic status than the subjects who had stable lives. Cognitive function of free meal receivers was lower than the people with stable lives in the most cognitive factors. Especially age of 50 to 64, pre-aged group, who had been receiving free meals, showed lowest cognitive level than the other age groups. Among 7 cognitive factors, there was the greatest difference in attention and calculation between 2 groups. Percentage of 'conclusive dementia' among the free meal receivers was statistically higher than the people with stable lives and that of 'conclusively normal' was statistically lower in free meal receivers comparing with the people with stable lives. Moreover, socioeconomic factors like income, former job, marital status, housing and education, blood pressure and physical activity were significantly related to the subject's cognitive function.

• Korean Institute of Interior Design Journal
• /
• v.26 no.3
• /
• pp.13-24
• /
• 2017

Promoting barrier-free living environments is necessary in residential facilities on which senior citizens rely most of their lives. Safety of elder people can be reassured by a certification system based on the existing barrier-free certification program in public facilities. The range of certification does not cover all living area, but is limited to the bathroom, which is a sanitary space. Given the nature of BIM modeling, an existing multi-family building was selected and modeled, as the certification process requires all the necessary information and various viewport. BIM modeling of the bathroom was conducted in accordance with the requirements of the certification for barrier-free living environment. The results suggested that there is a need for a removal of thresholds, larger doors, better selection of finishing materials, sufficient room for a wheelchair, larger space next to the toilet, and the availability of an emergency bell and grab bars. Such information supports the potentials of BIM modeling, and it is expected that an automated certification system would be established in the foreseeable future.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.54 no.1
• /
• pp.23-30
• /
• 2021

To examine the optimal temperature, light intensity, and shell-type for shell-living conchocelis production, we tested the shell infiltration of free-living conchocelis fragments under various environmental conditions. Under a combination of various temperatures (10, 15, 20, 25 and 30℃) and light intensities (1, 5, 10, 20, 40, and 80 μmol m-2 s-1), the optimal infiltration conditions of the evaluated three Pyropia species were 20-25℃ and 5-80 μmol m-2 s-1 for P. yezoensis, 20-30℃ and 20-80 μmol m-2 s-1 for P. seriata, and 20-25℃ and 20-80 μmol m-2 s-1 for P. dentata. The infiltration efficiency of free-living conchocelis for different shell types was greater in Korean and Chinese oyster Crassostrea gigas shells than that in scallop Argopecten irradians and clam Meretrix lusoria shells. These results suggest that oyster shells are suitable substrates for shell-living conchocelis production. In conclusion, the present results for optimal infiltration conditions for free-living conchocelis of the three examined Pyropia species will contribute significantly to the production of stable shell-living conchocelis.

• ALGAE
• /
• v.22 no.1
• /
• pp.23-29
• /
• 2007

Free-living heterotrophic stramenopile flagellates, which lack chloroplasts, were encountered in deep-sea sediments of Gippsland Basin (Australia) and classified into 10 species (8 genera, 5 families, 3 orders). Their descriptions were based on living specimens by light microscopy. Those species rarely found in this study were Bicosoeca gracilipes, Caecitellus parvulus, Cafeteria minuta, Cafeteria roenbergensis, Pseudobodo tremulans, Spumella sp., Paraphysomonas sp., Actinomonas mirabilis, Ciliophrys infusionum and Developayella elegans. Their morphological characters and geographic distribution are presented.

• Nutritional Sciences
• /
• v.5 no.3
• /
• pp.146-154
• /
• 2002

The aim of this study was to investigate the effects of household type on the health- and food-related behavior of the elderly. The survey was conducted on 304 free-living elderly persons in Chun-Chon city in 1999. The subjects were divided into three groups by their household types : living alone (n=35), living with their spouses (n=59), and living with their extended family (n=191). The results were obtained by personal interviews using questionnaires. Compared to the group living with their spouses and/or extended families, the elderly living alone were : less satisfied with the amount of monthly pocket money available; less involved in social activities; taking less vitamin supplements; doing less physical exercise; more frequently skipping meals and dining out; less frequently consuming meats, vegetables, fruits and seaweed; and having the highest rate of depression. The elderly living with their spouses tended to have the best physical functions and the greatest ability to carry out basic daily activities. The elderly living with their extended families had a higher incidence of diseases compared to the other groups. The preferred snacks were cookies for the elderly living alone, and fruits for the other two groups. In summary, it has been shown that household type should be taken into consideration for formulating adequate future strategies for effective health and nutrition programs.

• Parasites, Hosts and Diseases
• /
• v.30 no.2
• /
• pp.151-153
• /
• 1992

We have successfully cryopreserved free-living amoebae in order to maintain them feasibly under the conditions in our laboratory. The viability of trophozoites was higher when frozen by slow cooling (overall $0.7^{\circ}C$/min) than by fast cooling (overall $1.3^{\circ}C$/min). Glycerol and dimethylsulfoxide at the final concentration of 7.5% each was used for cryopreservation of free-living amoebae trophosoites. The survival rate was 2∼39% after storage in the liquid nitrogen for 60 days. Gross cultural or morphological changes were not noted in trophozoites thawed from frozen suspensions.