• The Plant Pathology Journal
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• 제35권6호
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• pp.674-683
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• 2019

Some species of the Trichoderma genus are reported as the major problem in oak wood mushroom production in Korea. In spite of economic loss by the fungi, scientific information on airborne Trichoderma species is not much available. To generate information for disease management development we analyzed airborne Trichoderma. A total of 1,063 fungal isolates were purely obtained from indoor air sampling of cultivation houses used for oak wood mushroom using sawdust media. Among the obtained isolates, 248 isolates were identified as Trichoderma fungi including T. harzianum, T. atroviride, T. citrinoviride, and T. pseudokoningii, by morphological and molecular analysis. T. harzianum was dominant among the four identified species. All the four Trichoderma species grew fast on solid nutrient media tested (potato dextrose agar [PDA], malt extract agar [MEA], Czapek's Dox + yeast extract agar [CYA] and cornmeal dextrose agar). Compact mycelia growth and mass spore production were better on PDA and CYA. In addition, T. harzianum and T. citrinoviride formed greenish and yellowish mycelium and spores on PDA and CYA. Greenish and yellowish pigment was saturated into PDA only by T. pseudokoningii. These four Trichoderma species could produce extracellular enzymes of sawdust substrate degradation such as β-glucosidase, avicelase, CM-cellulase, amylase, pectinase, xylanase, and protease. Their mycelia inhibited the growth of oak wood mushroom mycelia of two tested cultivars on dual culture assay. Among of eleven antifungal agents tested, benomyl was the best to inhibit the growth of the four Trichoderma species. Our results demonstrate that the airborne Trichoderma fungi need to be properly managed in the cultivation houses for safe mushroom production.

• Journal of Forest and Environmental Science
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• 제34권3호
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• pp.199-208
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• 2018

Quercus floribunda (Tilonj oak) is among the five species of Quercus and an evergreen tree found in Kumaun Himalayan region. Timing of germination is a crucial event determining the success of seedling establishment and survival. The aims of the study were to investigate the effect of sowing date on the germination and morphological responses of Tilonj oak (Quercus floribunda), cultivated during the month of August-September, with supplementary irrigation. The experiment was conducted by sowing seeds at two dates with one month interval in glass house conditions at DSB Campus, Nainital, Uttarakhand, India. Present study revealed that higher germination percentage (46.67 %) was recorded at sowing time $S_2$ as compared to the sowing time $S_1$ (32.86 %). Germination percentage as well as seedling growth were affected by sowing date though the differences were insignificant. At both the sowing dates, highest germination percentage was recorded for large seeds (32.86-46.67 %) followed by medium (31.43-33.33 %) and lowest germination (6.67-7.14 %) was recorded for small size seeds. In all parameters assessed, sowing time $S_2$ had the best performance in combination with large seed size. Thus, September is suitable month for best germination, growth and seedling vigour and large seed size is recommended to silviculturists and tree planters. The information on seed germination and seedling growth is vital both for conservation and rehabilitation of degraded lands.

• Journal of the Korean Wood Science and Technology
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• 제26권3호
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• pp.53-62
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• 1998

In this research, 7 species of white rot fungi were used for determining the resistance against pentachlorophenol (PCP). Three fungi with good PCP resistance were selected for evaluating the biodegradability, and biodegradation mechanism by HPLC and GC/MS spectrometry. Among 7 fungi, there were significant differences on PCP resistance on 4 different PCP concentrations. In the concentrations of 50 and 100ppm ($\mu$g of PCP per g of 2% malt extract agar), most fungi were easily able to grow, and well suited to newly PCP-added condition, but in that of more than 250ppm, the mycelia growths of Ganoderma lucidum 20435, G. lucidum 20432, Pleurotus ostreatus, and Daldinia concentrica were significantly inhibited or even stopped by the addition of PCP to the culture. However, Trametes versicolor, Phanerochaete chrysosporium, and Inonotus cuticularis still kept growing at 250ppm, indicating the potential utilization of wood rot fungi to high concentrated PCP biodegradation. Particularly, P. chrysosporium even showed very rapid growth rate at more than 500ppm of PCP concentration. Three selected fungi based on the above results showed an excellent biodegradability against PCP. P. chrysosporium degraded PCP up to 84% on the first day of incubation, and during 7 days, most of added PCP were degraded. T. versicolor also showed more than 90% of biodegradability at 7th day, and even though the initial stage of degradation was very slow, I. cuticularis has been approached to 90% at 21 st day after incubation with dense growing pattern of mycelia. Therefore, the PCP biodegradability was definitely dependent on the rapid suitability of fungi to newly PCP-added condition. In addition, the PCP biodegradation by filtrates of P. chrysosporium, T. versicolor, and I. cuticularis was very minimal or limited, suggesting that the extracellular enzyme system may be not so significantly related to the PCP biodegradation. Among the biodegradation metabolites of PCP, the most abundant one was pentachloroanisole which resulted in a little weaker toxicity than PCP, and others were tetrachlorophenol, tetrachloro-hydroquinone, benzoic acid, and salicylic acid, suggesting that PCP may be biodegraded by several sequential reactions such as methylation, radical-induced oxidation, dechlorination, and hydroxylation.

• 한국산림과학회지
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• 제35권1호
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• pp.47-55
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• 1977

우리나라에서는 최근에 고도의 경제성장(經濟成長)으로 인(因)한 국토개발사업(國土開發事業)이 전국토(全國士)에서 시행(施行)되고 있다. 특(特)히 경부고속도로(京釜高速道路)를 비롯한 각종(各種) 고속도로(高速道路)의 건설(建設)과 산업기지(産業基地) 및 택지(宅地) 공장부지(工場敷地)의 조성공사(造成工事) 등으로 자연(自然)비탈면이 많이 파괴(破壞) 훼손(毁損)되었으므로 이를 복구(復舊) 녹화(綠化)하기 위한 녹화공법(綠化工法)으로서 주로 식생공법(植生工法)이 채용되고 있으나 절토(切土)나 성토공사(盛土工事)로 인하여 인위적(人爲的)으로 만들어진 인공(人工) 비탈면의 안정에 미치는 식생(植生)의 영향에 관한 문제가 아직 종합적으로 보고(報告)된 바 있다. 그러므로 본(本) 연구(硏究)에서는 우선 비탈면의 피해형태(被害形態)를 분석(分析)하고, 식생(植生)이 비탈면의 물 수지(收支)에 미치는 영향을 분석(分析)하였으며, 또 식생(植生)이 표면침식방지(表面浸蝕防止) 및 표층붕괴(表層崩壞)에 미치는 영향을 분석(分析)하였다.

• Journal of Ecology and Environment
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• 제29권4호
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• pp.405-410
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• 2006

Landscape changes for 20 years between 1981 and 2001 in the Wangsuk stream basin located on the central Korea were investigated on the basis of physiognomic vegetation map made from the aerial photograph interpretation and field check. Changes of landscape structure were noticeable in agricultural field and forest landscape elements. Changes in the agricultural fields due to transformation of agricultural pattern into the institutional agriculture dominated landscape change, although urbanization also contributed to such change. The former change due to change of food production structure originated from socio-economic development during this period and the latter to the overpopulation of Seoul. As energy sources for heating and cooking, fertilizer, and fodder for livestock transform from plant materials to fossil fuel, manufactured one, and grain, succession of forest escaped from direct human disturbance dominated change of landscape structure in forested land. Differently from the positive landscape change in the upper area, change in the lower area deteriorated landscape quality by increasing artificial land. It was estimated that such landscape deterioration in the Wangsuk stream basin would influence water quality of the stream. In order to realize sustainable land-use against such environmental degradation, systematic environmental management based on landscape ecological perspective such as "an eco-plan for creating riparian vegetation belt," which is under preparation by Ministry of Environment, was recommended.

• 보존과학회지
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• 제31권1호
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• pp.13-20
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• 2015

목조문화재는 수분의 영향으로 열화의 가속화가 진행되기 때문에 전통적으로 표면을 수분으로부터 차단하기 위해 들기름 및 동유와 같은 유지류를 도포하였다. 그러나 목조문화재에 적합한 유지류 코팅 방법 및 성능에 대한 체계적인 연구가 부족한 실정이다. 본 연구는 천연 유지류 3종과 오일스테인 2종의 목재의 방향별, 공시재의 시간 경과 및 유지류의 중탕 여부에 따른 방수 성능을 평가하여 현장 적용 가능성을 판단하였다. 건전 목재의 섬유방향에서 상온의 천연유지류 코팅제 방수성능은 대조군과 비교하여 약 79.2%를 차단하였으며, 특히 동유는 90.5%의 수분을 차단할 정도로 우수한 성능을 나타냈다. 2년간 실내에서 컨디셔닝을 실시한 경우 유지류의 방수 성능은 약 8.8% 감소하였다. 유지류를 중탕하여 사용할 경우 약 5.0%의 방수 성능이 향상되며, 특히 아마인유는 중탕하여 사용할 경우 약 9.6%의 방수 성능을 개선할 수 있다.

• 한국버섯학회지
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• 제16권4호
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• pp.272-278
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• 2018

본 연구에서는 원목재배용 표고(Lentinula edodes) 품종에 대한 목질섬유소 분해능을 검정하였다. 5개의 국산품종(천백고, 산조 303호, 풍년고, 백화향, 수향고)을 대상으로 malt extract broth(MEB) 배지에 lignin의 첨가에 따른 RBBR(Remazol Brilliant Blue R) 탈색능을 조사한 결과, 산조 303호와 풍년고가 배양 5일째부터 우수한 분해능을 보였다. 섬유소 분해효소인 MnP와 laccase의 활성은 풍년고가 배양 7일째 MnP 활성이 2,809U/mg, laccase 활성이 2,230U/mg으로 나타났고, 산조 303호가 배양 11일째 MnP 활성이 2,673U/mg, laccase 활성이 2,049U/mg으로 최고 활성을 나타났으며, lignin을 첨가하였을 때 효소의 활성이 증가하였다. 산조 303호, 풍년고와 수향고는 배양 5주 만에 filter paper의 분해정도를 육안으로 확인이 가능할 정도로 분해능이 우수하였다.

• Journal of Ecology and Environment
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• 제43권3호
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• pp.320-331
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• 2019

Background: Dioecious plant species having both male and female plants have been investigated regarding sex-related characteristics such as sex ratio, sex-differential resource requirements, and spatial segregation of the sexes. Habitat loss and fragmentation are major threats to the survival of plant populations, but dioecious species are particularly more prone to such habitat degradation than non-dioecious species because of their dimorphic sexual system. We examined the sex-related demographics of two Ilex cornuta populations being different regarding land use history. Methods: During 2016-2017, we examined I. cornuta trees with a basal diameter ${\geq}1.5cm$ in the Yongsu-ri population (YS population) and the Gotjawal Provincial Park population (GP population). Plant sex (male, female, or unsexed) was identified. The tree size (basal diameter and height of the main stem), clonal production (the ramet numbers per genet), and vitality for each clone were measured. The associations between population, sex, tree size, clonal production, and vitality were examined using ANOVAs and contingency table analyses. Finally, point pattern analyses using O-ring statistics were conducted to assess spatial patterns. Results: Upon excluding unsexed trees, the YS population with 74 trees was significantly male-biased (0.66), while the GP population with only 26 trees had a 1:1 sex ratio. In both populations, males and females did not differ in tree size. Although the mean number of ramets differed significantly between populations, females tended to produce more ramets than males. The proportion of weak trees was significantly higher in the YS than in the GP population. Neither population showed evidence of spatial segregation of the sexes. Conclusions: The two populations of dioecious I. cornuta are characterized by the small number of trees and relatively high frequencies of non-reproductive trees. Both indicate that these populations are quite susceptible to environmental and genetic stochasticity. On the other hand, the differences between populations in sex ratio, clonal production, and vitality suggest that conservation efforts for I. cornuta need to be population-specific. In order to help recover and enable this vulnerable species to persist, it is necessary to find ways to enhance their sexual reproduction and simultaneously reduce habitat disturbances due to anthropogenic activities.

• Journal of the Korean Wood Science and Technology
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• 제31권4호
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• pp.44-49
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• 2003

수용성 방부처리(AAC, CCA-3, ACQ, CuAz) 소나무 시편의 기상열화에 대한 저항성을 평가하기 위하여 인공촉진열화 방법을 사용하였다. 기상열화에 의한 재색 및 중량변화, 물리적 표면열화, 목재조직의 변화를 종합적으로 고려할 때, AAC 처리시편을 제외한 ACQ, CCA, CuAz 처리시편은 기상열화에 대한 저항성이 있음을 알 수 있었다. 이러한 몇몇 방부처리재가 기상열화에 대하여 저항성을 나타내는 요인탐색을 위해 기상열화 산물을 분석하였는데, 그 결과, ACQ, CCA, CuAz 처리시편에서는 낮은 리그닌 함량이 측정되어 기상열화 저항성을 가지는 처리시편은 리그닌 열화가 적게 일어남을 나타냈다. 이는 방부제 유효성분이 목재 주성분 중 기상열화에 민감한 리그닌 구조에 정착함으로써 리그닌 열화에 대한 저항성이 발현되었기 때문으로 판단된다.

• Journal of Forest and Environmental Science
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• 제26권2호
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• pp.83-94
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• 2010

Chinese fir (Latin name: Cunninghaimia lanceolata) is one of the major commercial coniferous trees. Most of Chinese fir forests are managed in successive rotation sites, which lead productivity to decline. Autotoxicity is the important reason for soil degradation of Chinese fir plantation, especially, phenolic acids are considered as the major allelopathic toxins which induce autotoxicity in Chinese fir rotation stands. We performed here proteomic approach to investigate the response of proteins in Chinese fir leaves to salicylic acid. The tube plantlets of Chinese fir clone were treated with 120 mg/L salicylic acid for 1, 3 and 5th day. 2-DE, coupled with MALDI-TOF-TOF/MS, was used to separate and identify the responsive proteins. We found 12, 7, and 12 candidate protein spots that were up- or down-regulated by at least 2.5 fold after 1, 3, and 5th day of the stress, respectively. Of these protein spots, 16 spots were identified successfully. According to the putative physiological functions, these proteins were categorized into five classes (1) the proteins involved in protein stability and folding, including 26S proteome, Grp78, Hsp70, Hsp90 and PPIase; (2) the protein involved in photosynthesis and respiration, including OEC 33 kDa subunit, GAPDH; (3) the protein related to cell endurance to acid, F-ATPase; (4) the protein related to cytoskeleton, tubulin; (5) the protein related to protein translation: prolyl-tRNA synthetase. These results give new insights into autotoxic substance stress response in Chinese fir leaves and provide preliminary footprints for further studies on the molecular signal mechanisms induced by the stress.