• Title/Summary/Keyword: forensic science

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Mutation Cases in the Korean Population using 23 Autosomal STR Loci Analysis

  • Kim, Jeongyong;Kim, Hyojeong;Lee, Ja Hyun;Kim, Hyo Sook;Kim, Eungsoo
    • Biomedical Science Letters
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    • v.27 no.2
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    • pp.105-110
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    • 2021
  • Short Tandem Repeats (STR) analysis which characterized by genetic polymorphism has been widely used in the forensic genetic fields. Unfortunately, mutation occurred in various STR loci could make it difficult to interpret STR data. Thus, the mutation rate of STR loci plays an important role for the data interpretation in human identification and paternity test. To verify the mutation of the STR loci in the Korean population, 545 trio sets (father, mother, and child) were analyzed with two commercial STR kits that include the 23 autosomal STR loci (D1S1656, TPOX, D2S441, D2S1338, D3S1358, FGA, D5S818, CSF1PO, D7S820, D8S1179, D10S1248, TH01, D12S391, VWA D13S317, D16S539, D18S51, D19S433, D21S11, D22S1045, SE33, Penta E and Penta D). As a result, 36 mutations were observed in 14 STR loci. The types of mutation were also classified by the increase or decrease of the alleles. The overall mutation rate was 1.4×10-3, and the paternal mutation rate was four times higher than that of the maternal. This study will provide more detailed criterion for human identification by the mutation rate of STR loci in the Korean population.

Comparative study of serological detection methods on old bloodstain samples (오래된 혈흔 시료에 대한 혈청학적 식별법의 비교 연구)

  • Lee, Minji;Ahn, Eu-Ree;Kim, Da-Hye;Shin, Heejin;Jung, Ju Yeon;Lee, Sung-Jin;Chun, Byung-Won
    • Analytical Science and Technology
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    • v.31 no.5
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    • pp.201-207
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    • 2018
  • Blood is a commonly found body fluid at crime scenes, and plays an important role in identifying suspects and in the reconstruction of crime scenes. Although serological detection of blood has been widely used in the field of forensic science, research on the detection of old bloodstains is scarce. This work aimed to compare various methods for the detection of old bloodstains and validate the reliability of their results. Four presumptive tests-Tetramethylbenzidine, $Bluestar^{(R)}$, Leucomalachite Green, Kastle-Meyer tests-and two confirmatory tests-Fecal Occult Blood (FOB) and Rapid Stain $Identification^{(TM)}-Blood$ ($RSID^{TM}-Blood$) tests-were compared. Bloodstain samples from post-mortem cases were collected on gauzes and then stored at room temperature for periods from 7 to 30 years. All the presumptive tests were positive, even for the 30-year-old sample. However, FOB and $RSID^{TM}-Blood$ provided false negative results for some samples stored for 17 years or more (1988 to 2001). The results indicate that FOB and $RSID^{TM}-Blood$ are not reliable for the detection of old bloodstains. These findings can be useful in the selection of an appropriate detection method for serological testing of old bloodstains. In addition, the information will be useful background knowledge when applied in the field of forensic practice.

Identification of the Trace Evidence by UV/VIS Microspectrophotometry (현미 자외/가시광선영역 분광광도계에 의한 미세시료의 분석)

  • Shon, Sung-Kun;Park, Ha-Sun;Lee, Jin-Sook;Park, Sung-Woo;Cho, Sung-Hye
    • Analytical Science and Technology
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    • v.13 no.2
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    • pp.250-257
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    • 2000
  • Paint, fiber and dye which play a critcal role in proving the relationship between a suspect and a victim or a crime scene, are one of the most frequently encountered trace evidences at a forensic laboratory, however, in usual, because of infinitesimally small sizes of forensic samples, investigation of the spectroscopic characteristics of such samples is becomming more and more prevalent in forensic science as a non-destructive method. In this study, transmittance/reflectance profiles at ultraviolet-visible region (240-780nm), were investigated by UV/visible microspectro- photometer and used to analyze the spectral characteristics of different types of 14 microfibers, 12 inks of four colors and 44 automotive paints of two colors. Good results for discrimination were given from spectra of these samples due to the characteristic bands in uv/vis region, respectively.

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Analysis of Automotive Paints using Pyrolysis-Gas Chromatography (열분해-가스크로마토그라피에 의한 자동차 페인트 분석)

  • Shon, Sung-Kun;Park, Ha-Sun;Lee, Jin-Sook;Hong, Sung-Wook;Park, Sung-Woo;Cho, Sung-Hye
    • Analytical Science and Technology
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    • v.13 no.1
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    • pp.101-107
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    • 2000
  • The automotive paints could be generally differentiated by color, layer sequence and chemistry of the paint layers comprising each of the topcoat and the primer system. The successful identification of hit-andrun a and traffic accidental vehicles from evidential paint fiagments is greatly facilitated with a comprehensive laboratory collection of reference paint samples and the technique for direct analysis without sample preparation. The Pyrolysis-Gas Chromatography(PGC) is a precise and reliable method for performing both quantitative and qualitative analysis of polymeric materials and forensic samples. Our Forensic Laboratory is conducting the examination and identification of 73 reference paint samples; 4 colors of each domestic automotive make that is popular in Korea, by Curie Point Pyrolyzer(JHP-3) and GC with capillary column(ultra alloy-5). This method can be used not only to compare paint traces with their suspected sources, but also to identify the type, make and model of the automotive car.

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The Development of Anti-Forensic Tools for Android Smartphones (안드로이드 스마트폰을 위한 앤티-포렌식 도구 개발)

  • Moon, Phil-Joo
    • The Journal of the Korea institute of electronic communication sciences
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    • v.10 no.1
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    • pp.95-102
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    • 2015
  • Smartphone is very useful for use in the real world, but it has been exposed to a lot of crime by smartphone. Also, it occurs attempting to delete a data of smartphone memory by anti-forensic tools. In this paper, we implement an anti-forensic tool used in the Android. In addition, tests to validate the availability of the anti-forensic tool by the Oxygen Forensic Suite that is a commercial forensic tool.

Analysis of a Third-Party Application for Mobile Forensic Investigation

  • Ryu, Jung Hyun;Kim, Nam Yong;Kwon, Byoung Wook;Suk, Sang Ki;Park, Jin Ho;Park, Jong Hyuk
    • Journal of Information Processing Systems
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    • v.14 no.3
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    • pp.680-693
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    • 2018
  • Nowadays, third-party applications form an important part of the mobile environment, and social networking applications in particular can leave a variety of user footprints compared to other applications. Digital forensics of mobile third-party applications can provide important evidence to forensics investigators. However, most mobile operating systems are now updated on a frequent basis, and developers are constantly releasing new versions of them. For these reasons, forensic investigators experience difficulties in finding the locations and meanings of data during digital investigations. Therefore, this paper presents scenario-based methods of forensic analysis for a specific third-party social networking service application on a specific mobile device. When applied to certain third-party applications, digital forensics can provide forensic investigators with useful data for the investigation process. The main purpose of the forensic analysis proposed in the present paper is to determine whether the general use of third-party applications leaves data in the mobile internal storage of mobile devices and whether such data are meaningful for forensic purposes.

On the Availability of Anti-Forensic Tools for Android Smartphones (안드로이드 스마트폰을 위한 앤티-포렌식 도구들의 활용성)

  • Moon, Phil-Joo
    • The Journal of the Korea institute of electronic communication sciences
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    • v.8 no.6
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    • pp.855-861
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    • 2013
  • Smartphone is very useful for use in the real life through the improvement of computing power, faster data rate and the variety of applications. On the other hand, using the smartphone has been exposed to a lot of crime. Also, it occurs attempting to delete a data of smartphone memory by anti-forensic tools. In this paper, we investigate and analyze the anti-forensic tools used in the Android smartphone to study the characteristics and techniques of anti-forensic tools. In addition, experiments are performed to validate the availability of anti-forensic tools by the Oxygen Forensic Suite that is a commercial forensic tool.

A Case of Single-step Mutations at Two Short Tandem Repeat loci (D13S317 and DXS10148) among Three Generations of a Korean Family

  • Byeong Ju Youn;Kyungmyung Lee;Cho Hee Kim
    • Biomedical Science Letters
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    • v.28 no.4
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    • pp.327-333
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    • 2022
  • The DNA profiling of short tandem repeat (STR) markers is a powerful tool for forensic identification and forensic paternity testing. However, STR loci are susceptible to mutation that cause mismatches between parents and children when paternity is tested. Herein, we examined paternity disputes with 23 autosomal STR loci using two commercial human identification kits and revealed successive mismatches at the D13S317 locus across three generations of a Korean family. Additionally, we investigated 12 X-chromosomal STRs and discovered an inconsistency at the DXS10148 locus between the father and daughter of the same Korean family. Furthermore, we confirmed STR genotypes at the D13S317 and DXS10148 loci of the family using sequencing analysis. Consequently, we identified a successive single-step mutation at the D13S317 locus and one single-step mutation at the DXS10148 locus in three generations of the Korean family. Therefore, this case study may be useful for interpreting and understanding forensic paternity tests.

Identification of Impurities in a Sample of Illicitly Synthesized Methamphetamine

  • Kim, Sung-Hong;Kwon, Woo-Jung;Ryoo, Jae-Jung;Ko, Beom-Jun;Suh, Yong-Jun;In, Mun-Kyo
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.285.2-286
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    • 2003
  • Analysis of illicit methamphetamine samples seized in Korea is discussed. The samples are extracted with the small portion of ethyl acetate under neutral conditions and the extracts are analyzed by GC-MS. Several impuritiy peaks are found in each chromatrogram. Eight compunds (1,2-Dimethyl-3-phenylaziridine, amphetamine oxime, ephedrine, N-formylmethamphetamine, N-acetylmethamphetamine, acetylephedrine, 3,4-dimethyl-5-phenyl-2-oxazolidone, methamphetamine dimer) are identified impurities in illicit methamphetamine and the identity of the impurity is conformed synthesis. (omitted)

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