• Journal of the Korean Magnetic Resonance Society
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• v.17 no.2
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• pp.81-85
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• 2013

Various fluorine-containing materials are used in electronic devices like LCD display panels and Li-ion batteries. The structural conformation of fluorine in fluorinated materials is an important contributing factor that influences the chemical and physical properties. The conformation can be changed by heat and stress during manufacture or use. Understanding the conformational changes is critical for understanding the performance and durability of electronic devices. Solid-state NMR spectroscopy could be widely used for the analysis of various fluorine-containing materials for electronic devices. However, conventional CPMAS probes cannot be used for in-situ analysis of fluorine-containing electronic devices like LCD panels and Li-ion batteries. In this paper, we show the design, construction, and optimization of a $^{19}F-^{13}C$ double-resonance solid-state NMR probe for a 400MHz wide-bore magnet with a flat square coil for in-situ analysis of fluorine-containing electronic devices without observing fluorine background signals. This custom-built probe does not show any fluorine background signals, and can have higher efficiency for lossy samples.

• KSBB Journal
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• v.11 no.6
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• pp.704-711
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• 1996

An optimal process design of a foreign protein production system was carried out using a bioprocess flowsheeting software, BioPro Designer, with a capability of economic analysis. The flowsheeting program was applied to a production system of the tailspike protein of Salmonella phage P22, and helped save time and efforts in selecting an optimal process. A wild type tailspike and two types of mutant tailspikes, tsf G244\longrightarrow,R and Su A334\longrightarrowV, were considered in this study to show that the folding characteristics of foreign protein produced inside host influenced the selection of the best production system. An optimal production system for mature tailspike was chosen under the criterion of capital investment per unit mass of mature protein recovered.

• Proceedings of the Korean Magnetic Resonance Society Conference
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• 2002.08a
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• pp.82-82
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• 2002

• Molecules and Cells
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• v.38 no.9
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• pp.789-795
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• 2015

A chromosome territory is composed of chromosomal subdomains. The internal structure of chromosomal subdomains provides a structural framework for many genomic activities such as replication and DNA repair, and thus is key to determining the basis of their mechanisms. However, the internal structure and regulating proteins of a chromosomal subdomain remains elusive. Previously, we showed that the chromosome territory expanded after BAF53 knockdown. Because the integrity of chromosomal subdomains is a deciding factor of the volume of a chromosome territory, we examined here the effect of BAF53 knockdown on chromosomal subdomains. We found that BAF53 knockdown led to the disintegration of histone H2B-GFP-visualized chromosomal subdomains and BrdU-labeled replication foci. In addition, the size of DNA loops measured by the maximum fluorescent halo technique increased and became irregular after BAF53 knockdown, indicating DNA loops were released from the residual nuclear structure. These data can be accounted for by the model that BAF53 is prerequisite for maintaining the structural integrity of chromosomal subdomains.

• Journal of Plant Biotechnology
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• v.2 no.1
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• pp.1-12
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• 2000

• Proceedings of the Zoological Society Korea Conference
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• 1997.10b
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• pp.281.2-281
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• 1997

No Abstract, See Full Text

• Animal cells and systems
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• v.13 no.4
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• pp.405-409
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• 2009

When DNA double-strand breaks (DSBs) were induced in mammalian cells, many DNA damage response proteins are accumulated at damage sites to form nuclear foci called IR-induced foci. Although the formation of foci has been shown to promote repair efficiency, the structural organization of chromatin in foci remains obscure. BAF53 is an actin-related protein which is required for maintenance of chromosome territory. In this study, we show that the formation of IR-induced foci by $\gamma$-H2AX and 53BP1 were reduced when BAF53 is depleted, while DSB- activated ATM pathway and the phosphorylation of H2AX remains intact after DNA damage in BAF53 knockdown cells. We also found that DSB repair efficiency was largely compromised in BAF53 knockdown cells. These results indicate that BAF53 is critical for formation of foci by $\gamma$-H2AX decorated chromatin at damage sites and the structural organization of chromatin in foci is an important factor to achieve the maximum efficiency of DNA repair.

• Journal of the Korean Magnetic Resonance Society
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• v.16 no.2
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• pp.147-161
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• 2012

Human melanocortin-4 receptor (hMC4R) among MC-Rs, expressed in the brain, is in charge of the control on energy homeostasis and food intake. The structure and function of human MC4R have been studied to understand their essential function and roles. To investigate the structure and function, it is necessary to prepare sufficient amounts of proteins. However, their expression and purification is demanding and time-consuming due to their innate insoluble and toxic properties. The heterozygous mutations of hMC4R, exchange of Asp 90 to Asn located in second transmembrane, cause severe obesity in human. To obtain purified hMC4R wt-TM2 for structural studies, it was first over-expressed and purified by fast protein liquid chromatography (FPLC) and then solution NMR studies were performed to get high-resolution spectra. In here, we established optimized purification scheme to get more purified target peptide.

• Bulletin of the Korean Chemical Society
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• v.30 no.12
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• pp.3123-3126
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• 2009

• Korean Journal of Microbiology
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• v.25 no.1
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• pp.52-56
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• 1987

The evidences that Lam B protein of E. coli is used as a receptor for infections of bacteriophage N4 as well as bacteriophage lambda were obtained from the following experimental results. First, all of the isolated lambda resistant dlones possessing foreign DNA fragments in the plasmids were also resistant to bacteriophage N4, but not to bacteriophage $\phi$ 80, T4 and T7. Second, when the plasmid DNA was treated with various restriction enzymes and ligated to delete the total or a portion of the foreign DNA fragments, the deleted plasmids lost the resistant activities to lambda and N4, simultaneously. Third, after amplification of Lam B protein about 200 times by inducing the protein using maltose as a sole carbon source, the host E. coli became sensitive to both lambda and N4.