• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.1
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• pp.77-88
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• 2010

In order to ensure good animal health and performance, it is essential to produce forages with high feeding value and good hygienic quality. However, huge amounts of forages consumed by ruminants are contaminated with mold prior to harvest or during storage as hay, straw or silage. These mold can grow in forages only when nutrients are available, correct temperature exist, oxygen is present, and unbound water is available. Fungal 'species can be divided into two groups: field fungi and storage fungi. Field fungi invade the forages while the crop is still in the field, require high moisture conditions, and are such as species of Fusarium, Alternaria, Clodosporium, Diplodia, Gibberrella and Helminthosporium. Storage fungi invade forages during storage and need less moisture than field fungi. These such as species of Aspergillus and Penicillium usually do not occur any problem before harvest. Mold growth can spoil the nutritional aspects of the forages and also results in secondary metabolites that are highly toxic to animal, humans and plants. Moldy feeds are less palatable and may reduce dry matter intake. This, in turn, leads to a reduction of nutrition intake, reducing weight gains or milk production. Performance losses of 5 to 10 percent are typical with moldy feeds. Mycotoxins are toxic substances produced by fungi (molds) growing on crops in the field or storages. While greater than 400 mycotoxins have been chemically identified, the biological or veterinary medical impact of only several mycotoxins is known. Mycotoxins have attracted considerable attention as potential causes for poor performance and health disorders in domestic livestock. They can be carcinogenic, hepatotoxic, hematotoxic, immunosuppressive, estrogenic, or mutagenic. So, feeding moldy forages has adverse effects on animal health and milk consumers. Also, this author reported that rice straw hay was contaminated mycotoxigenic fungi such as Penicillium roqueforti and Fusarium culmorum in Korea. Therefore, it is an urgent need to develop an improved post harvest storage method to reduce nutrient loss and mycotoxin contamination of forages, which will have a positive impact on human health.

• Weed & Turfgrass Science
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• v.3 no.1
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• pp.13-18
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• 2014

This study was conducted to develop an eco-friendly control method for water foxtail in the field sowing barley seeds before rice harvesting. When sea water was applied pre-emergent, 1 and 2 leaves, little injury was observed on barley. Percent of water foxtail control with sea water (100%) was more than 50% at 1 leaf application timing. When sodium chloride was applied 400, 800 and $1,600kg\;ha^{-1}$, little injury was observed on barley. Percent of water foxtail control with sodium chloride was 36-44% at 30 days after sowing. When ferrous sulfate was applied at 400, 800 and $1,600kg\;ha^{-1}$, emergent injury was observed 35-50% on barley. Percent of water foxtail control with ferrous sulfate was 48-79% at 30 days after sowing. When barley was sowed at 200, 300 and $400kg\;ha^{-1}$, seeding rate standing plants have many of the more crops, water foxtail occurrence was low. And, Forage yield of 300 kg ha-1 seeding in the highest. Based on the results, it is possible to control water foxtail in the field sowing barley seeds before rice harvesting more than 50%.

• Korean Journal of Organic Agriculture
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• v.20 no.1
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• pp.71-80
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• 2012

This experiments was conducted to investigate the influence on growth of rye, triticale with legume and investigated their productivity, feed value and stock carrying capacity in order to select the appropriate forage species in the central region of Korea. In the results, Rye+Red clover showed 5.2ton/ha of dry matter yield. But there was no significant difference with Triticale+Red clover and Rye in upland field. In paddy field, Rye+Hairy vetch showed 5.2ton/ha, but there was no significant difference with Triticale+Hairy vetch and Rye. Mixture effects with Rye, Triticale and Red clover, Hairy vetch by relative yield were more than 1.00 compared to monoculture, and their mixture effect was recognized in upland and paddy field. Relative feed value was highest in the applications of Triticale mixture in upland and paddy field. The average value of $K_{CP}$ and $K_{TDN}$ showed the highest one as 2.75head/ha/yr in Rye+Red clover in upland field and 2.84head/ ha/yr in Triticale+Hairy vetch in paddy field. According to the results, Rye+Red clover in upland field and Triticale+Hairy vetch in paddy field were considered to be the most appropriate winter forage crops for the central region of Korea in terms of productivity, feed value and stock carrying capacity.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.2
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• pp.109-116
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• 2007

Environmental stress is the major limiting factor in plant productivity. As an effort to solve the global food and environmental problems using the plant biotechnology, we have developed transgenic tall fescue (Festuca arundinacea Schreb.) plants via Agrobacterium-mediated gene transfer method. To develop transgenic tall fescue plants with enhanced tolerance to the environmental stresses, both CuZn superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) genes were incorporated in a pIG121 binary vector and the both of the genes were controlled separately by an oxidative stress-inducible sweet potato peroxidase 2 (SWPA2) premoter expressed in chloroplasts. Leaf discs of transgenic plants showed 10-30% less damage compared to the wild-type when they exposed to a wide range of environmental stresses including methyl viologen (MV), $H_2O_2$ and heavy metals. In addition, when $200{\mu}M$ MV was sprayed onto the whole plants, transgenic plants showed a significant reduction of visible damage compared to wild-type plants that were almost damaged. These results suggest that over expression of CuZnSOD and APX genes in transgenic plants might be a useful strategy to protect the crops against a wide range of environmental stresses.

• Korean Journal of Agricultural Science
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• v.4 no.1
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• pp.61-65
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• 1977

To select some salt tolerant pasture species for grassland establishment on the west coastal saline land in Korea, this work was carried out from March to October in 1976 with 5 pasture seeds mainly were introduced from Australia. On the soil salt content was 0.4%, all seeds germination were failure, but all species were survived on the 0.2% salt content land. The yield of forage were much better from Kikuyu grass and Pearl millet but poor from other species. The Meadow grass could be used for protect soil erosion by lot of tillers. By the result from this experiment, only Kikuyu grass and Pearl millet were possible to use for forage crops. But to continue this work, it is suggested to introduced more effective species such like Saltwater couch, Saltshore ryegrass, Puccinella, Salt bush, Blue buch, Samphire and so on.

• Animal Bioscience
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• v.34 no.10
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• pp.1616-1622
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• 2021

Objective: Bioactive compounds in ruminant products are related to functional compounds in their diets. Therefore, this study aimed to explore the effect of forage sources, Italian ryegrass (IR) silage vs corn silage (CS) in the total mixed ration (TMR), on milk production, milk composition, and phytanic acid content in milk, as well as on the extent of conversion of dietary phytol to milk phytanic acid. Methods: Phytanic acid content in milk was investigated for cows fed a TMR containing either IR silage or CS using 17 cows over three periods of 21 days each. In periods 1 and 3, cows were fed CS-based TMR (30% CS), while in period 2, cows were fed IR silage-based TMR (20% IR silage and10% CS). Results: The results showed that there were no differences in fat, protein, lactose, solids-not-fat, somatic cell count, and fatty acid composition of milk among the three experimental periods. There were no differences in the plasma concentration of glucose, triglycerides, total cholesterol, and nonesterified fatty acids among the three experimental periods, while the blood urea nitrogen was higher (p<0.05) in period 2. The milk phytanic acid content was higher (p<0.05) in period 2 (13.9 mg/kg) compared with periods 1 (9.30 mg/kg) and 3 (8.80 mg/kg). Also, the phytanic acid content in the feces was higher (p<0.05) in period 2 (1.65 mg/kg dry matter [DM]) compared with period 1 (1.15 mg/kg DM), and 3 (1.17 mg/kg DM). Although the phytol contents in feces did not differ among the three feeding periods, the conversion ratio from dietary phytol to milk phytanic acid was estimated to be only 2.6%. Conclusion: Phytanic acid content in cow's milk increases with increasing phytol content in diets. However, phytol might not be completely metabolized in the rumen and phytanic acid, in turn, might not be completely recovered into cow's milk. The change of phytanic acid content in milk may be positively correlated with the change of phytol in the diet within a short time.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.1
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• pp.1-9
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• 2022

This study was carried out to compare the DMY (dry matter yield) of IRG (Italian ryegrass) in the southern coastal regions of Korea due to seasonal climate scenarios such as the Kaul-Changma (late monsoon) in autumn, extreme winter cold, and drought in the next spring. The IRG data (n = 203) were collected from various Reports for Collaborative Research Program to Develop New Cultivars of Summer Crops in Jeju, 203 Namwon, and Yeungam from the Rural Development Administration - (en DASH). In order to define the seasonal climate scenarios, climate variables including temperature, humidity, wind, sunshine were used by collected from the Korean Meteorological Administration. The discriminant analysis based on 5% significance level was performed to distinguish normal and abnormal climate scenarios. Furthermore, the DMY comparison was simulated based on the information of sample distribution of IRG. As a result, in the southern coastal regions, only the impact of next spring drought on DMY of IRG was critical. Although the severe winter cold was clearly classified from the normal, there was no difference in DMY. Thus, the DMY comparison was simulated only for the next spring drought. Under the yield comparison simulation, DMY (kg/ha) in the normal and drought was 14,743.83 and 12,707.97 respectively. It implies that the expected damage caused by the spring drought was about 2,000 kg/ha. Furthermore, the predicted DMY of spring drought was wider and slower than that of normal, indicating on high variability. This study is meaningful in confirming the predictive DMY damage and its possibility by spring drought for IRG via statistical simulation considering seasonal climate scenarios.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.102-102
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• 2022

More than 74% of forage crops in Korea are cultivated in winter rice fields. Italian ryegrass (IRG) can be said to be a representative forage crop, but over 70% of its seeds are dependent on imports. In addition, there are concerns that the stable supply of research fees may be disrupted due to the effects of climate change, war, and other factors. Therefore, it is necessary to secure a large production area for stable supply. Korea's reclaimed land area is 135,000 ha and its agricultural area is 112,000 ha. Therefore, this study attempted to apply IRG to the domestic IRG seed industry through stable cultivation techniques on reclaimed land. In this study, IRG 'Kowiearly' varsity was cultivated in late October 2020 and early October 2021 in the Saemangeum reclaimed land. The seeding methods were conventional drill sowing seeding, new technology spot seedling (30 × 18 cm) and new technology drill sowing seeding. The amount of sowing was conventional drill sowing seeding 2.0(kg/10a), new technology spot seedling 1.5(kg/10a), and new technology drill sowing seeding 1.5(kg/10a). Fertilizer application amount is conventional drill sowing seeding(N-P₂O₅-K₂O) 9.0-12-12(kg/10a), and new technology spot seedling and drill sowing seeding were(N-P₂O₅-K₂O) 4.5-12-12(kg/10a) respectively. Fertilizer was applied accordingly. After that, in February, the conventional drill sowing seeding, new technology drill sowing seeding and spot seedling applied 4.0 and 2.2(kg/10a) of nitrogen supplement fertilizer, respectively. Before wintering, plant length was higher in 2022 than in 2021, but leaf number was higher in 2021. Heading time was April 30, 2021 and April 25, 2022. In heading time, plant length was 74 cm in 2021 and 67 cm in 2022, lower than in 2021. On the other hand, There was no difference in the number of panicle and the number of seeds in the 2021 harvester in all treatment plots, and, thus seed yield was no differ. However, the drill sowing seeding and spot seedling of the new technology were somewhat higher than the conventional drill sowing seeding. On the other hand, seed yield was decreased in all treatment plots compared to 2022 because of raifall deficiency in 2021.

• Korean journal of applied entomology
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• v.55 no.4
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• pp.459-464
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• 2016

An outbreak of the migratory locust, Locusta migratoria, in the environment-friendly reclaimed plantations of forage crops in Sanyimyeon, Haenam-gun, Jellanam-do, Korea in August 2014 caused severe damages to various crops. Owing to its first occurrence in the Korean history, the causes underlying the outbreak and phase-transition of the migratory locust were not known. It is critical to establish the genetic relationship of the migratory locust in Sanyimyeon, Haenam-gun with the other previously reported strains in the world in order to understand the mechanisms responsible for its outbreak. The gene sequences of the 16S ribosomal RNA (rRNA) and displacement-loop (D-loop) of the mitochondria of various regional species of the migratory locust were used to perform the phylogenetic analysis. Our results suggested that the migratory locusts in Sanyimyeon, Haenam-gun are closely related with the Eurasian strains of the northern lineage. In future, these two mitochondrial genes can be used for elucidating the genetic population structures in migratory locusts in various regions. In addition, the sequence information of these genes can be used to enhance our understanding of the genetic basis of the outbreak of migratory locusts.

• Journal of Microbiology and Biotechnology
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• v.11 no.6
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• pp.1093-1098
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• 2001

The gene coding for a Lepidoptera-specific insecticidal crystalline (or control) protein (ICP), recognized as cryIAc, from Bacillus thuringiensis subsp. kurstaki HD-73, was cloned into the vector pBluscript ll SK-, and then transformed in Escherichia coli $DH5{\alpha}$. The clone was named EBtIAc and the chimeric phagemid, as pEBtIAc. Hyperexpression of CryIAc protoxin was observed in the extract of the culture of E. coli harboring pEBtIAc. Crystalline protoxin was purified by differential solubility. It was dissolved in alkaline pH, and exposed to trypsin to be activated. The molecular weights of the pro- and activated toxins on SDS-PAGE were estimated to be ca. 130 kDa and 60 kDa, respectively. The toxicity was tested by force-feeding larvae of gypsi moth (Lymantria diapar) with trypsinized protoxin. Using the batch of biologically active form of the toxin as an immunogen, anti-CryIAc antiserum was raised in a New Zealand white rabbit. Immunoglobulin G was fractionated from the seam by Protein-A sepharose affinity chromatography. Immunoreactivity of the antibody was examined by dot and Westerns blottings. It has been found that the anti- CryIAc antibody recognized the purified toxin at a level below a nanogram in terms of quantity. Using the antibody some of Bt-corns were able to be differentiated from tons of corn kernels which were imported from America as forage crops.