Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effect of phytol in forage on phytanic acid content in cow's milk

  • Lv, Renlong (Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences) ;
  • Elsabagh, Mabrouk (Department of Animal Production and Technology, Faculty of Agricultural Sciences and Technologies, Nigde University) ;
  • Obitsu, Taketo (Graduate School of Integrated Sciences for Life, Hiroshima University) ;
  • Sugino, Toshihisa (Graduate School of Integrated Sciences for Life, Hiroshima University) ;
  • Kurokawa, Yuzo (Graduate School of Integrated Sciences for Life, Hiroshima University)
  • Received : 2021.02.22
  • Accepted : 2021.04.30
  • Published : 2021.10.01
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Abstract

Objective: Bioactive compounds in ruminant products are related to functional compounds in their diets. Therefore, this study aimed to explore the effect of forage sources, Italian ryegrass (IR) silage vs corn silage (CS) in the total mixed ration (TMR), on milk production, milk composition, and phytanic acid content in milk, as well as on the extent of conversion of dietary phytol to milk phytanic acid. Methods: Phytanic acid content in milk was investigated for cows fed a TMR containing either IR silage or CS using 17 cows over three periods of 21 days each. In periods 1 and 3, cows were fed CS-based TMR (30% CS), while in period 2, cows were fed IR silage-based TMR (20% IR silage and10% CS). Results: The results showed that there were no differences in fat, protein, lactose, solids-not-fat, somatic cell count, and fatty acid composition of milk among the three experimental periods. There were no differences in the plasma concentration of glucose, triglycerides, total cholesterol, and nonesterified fatty acids among the three experimental periods, while the blood urea nitrogen was higher (p<0.05) in period 2. The milk phytanic acid content was higher (p<0.05) in period 2 (13.9 mg/kg) compared with periods 1 (9.30 mg/kg) and 3 (8.80 mg/kg). Also, the phytanic acid content in the feces was higher (p<0.05) in period 2 (1.65 mg/kg dry matter [DM]) compared with period 1 (1.15 mg/kg DM), and 3 (1.17 mg/kg DM). Although the phytol contents in feces did not differ among the three feeding periods, the conversion ratio from dietary phytol to milk phytanic acid was estimated to be only 2.6%. Conclusion: Phytanic acid content in cow's milk increases with increasing phytol content in diets. However, phytol might not be completely metabolized in the rumen and phytanic acid, in turn, might not be completely recovered into cow's milk. The change of phytanic acid content in milk may be positively correlated with the change of phytol in the diet within a short time.

Keywords

Acknowledgement

This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number 26292138.

References

  1. Szumacher-Strabel M, El-Sherbiny M, Cieslak A, Szczechowiak J, Winiarska H. Bioactive lipid components from ruminant milk and meat: The new face of human health. In: Gupta VK, Tuohy M, editors. Biotechnology of bioactive compounds: sources and applications. Oxford, UK: John Wiley & Sons; 2015. pp. 599-629. https://doi.org/10.1002/9781118733103.ch25
  2. Wanders RJA, Komen J, Ferdinandusse S. Phytanic acid metabolism in health and disease. Biochim Biophys Acta Mol Cell Biol Lipids 2011;1811:498-507. https://doi.org/10.1016/j.bbalip.2011.06.006
  3. Roca-Saavedra P, Mario-Lorenzo P, Miranda JM, et al. Phytanic acid consumption and human health, risks, benefits and future trends: a review. Food Chem 2017;221:237-47. https://doi.org/10.1016/j.foodchem.2016.10.074
  4. Schroder M, Farideh Y, Walter V. Investigating the day-today variations of potential marker fatty acids for organic milk in milk from conventionally and organically raised cows. Eur Food Res Technol 2011;232:167-74. https://doi.org/10.1007/s00217-010-1374-8
  5. Schroder M, Nina LL, Ernest CT, Ensieh H, Farideh Y, Walter V. Phytanic acid concentrations and diastereomer ratios in milk fat during changes in the cow's feed from concentrate to hay and back. Eur Food Res Technol 2012;234:955-62. https://doi.org/10.1007/s00217-012-1710-2
  6. Halmemies-Beauchet-Filleau A, Kairenius P, Ahvenjarvi S, et al. Effect of forage conservation method on plasma lipids, mammary lipogenesis, and milk fatty acid composition in lactating cows fed diets containing a 60:40 forage-to-concentrate ratio. J Dairy Sci 2013;96:5267-89. https://doi.org/10.3168/jds.2013-6571
  7. Vanhatalo A, Kuoppala K, Toivonen V, Shingfield KJ. Effects of forage species and stage of maturity on bovine milk fatty acid composition. Eur J Lipid Sci Technol 2007;109:856-67. https://doi.org/10.1002/ejlt.200700023
  8. Lv R, Elsabagh M, Obitsu T, Sugino T, Kurokawa Y, Kawamura K. Effects of nitrogen fertilizer and harvesting stage on photosynthetic pigments and phytol contents of Italian ryegrass silage. Anim Sci J 2017;88:1513-22. https://doi.org/10.1111/asj.12810
  9. Lv R, Elsabagh M, Obitsu T, Sugino T, Kurokawa Y, Kawamura K. Effect of varying fermentation conditions with ensiling period and inoculum on photosynthetic pigments and phytol content in Italian ryegrass (Lolium multiflorum Lam.) silage. Anim Sci J 2020;91:e13309. https://doi.org/10.1111/asj.13309
  10. Breese EL. Exploitation of genetic resource through breeding: Lolium species. In: Mclover G, Bray RA, editors. Melbourne, Australia: Genetic Resources of Forage Plants CSIRO; 1983. pp. 275-88.
  11. Shao T, Ohba N, Shimojo M, Masuda Y. Dynamics of early fermentation of Italian ryegrass (Lolium multiflorum Lam) silage. Asian-Australas J Anim Sci 2002;15:1606-10. https://doi.org/10.5713/ajas.2002.1606
  12. Chen L, Guo G, Yu C, Zhang J, Shimojo M, Shao M. The effects of replacement of whole-plant corn with oat and common vetch on the fermentation quality, chemical com - position and aerobic stability of total mixed ration silage in Tibet. Anim Sci J 2015;86:69-76. https://doi.org/10.1111/asj.12245
  13. AOAC. Official methods of analysis. 15th ed. Arlington, VA, USA: Association of Official Analytical Chemists; 1990. https://doi.org/10.1016/0165-9936(90)87098-7
  14. Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. J Dairy Sci 1991;74:3583-97. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
  15. Sukhija PS, Paimquist DL. Rapid method for determination of total fatty acid content and composition of feedstuffs and feces. J Agric Food Chem 1988;36:1202-6. https://doi.org/10.1021/jf00084a019
  16. Liljenberg C, Odham G. Gas chromatographic determination of phytol in plant material. Physiol Plant 1969;22:686-93. https://doi.org/10.1111/j.1399-3054.1969.tb07424.x
  17. Takeda Y, Saito Y, Uchiyama M. Determination of pheophorbide a, pyropheophorbide a and phytol. J Chromatogr A 1983;280:188-93. https://doi.org/10.1016/S0021-9673(00)91558-9
  18. SAS Institute. SAS/STAT User's Guide, Version 9.1 Edition. Cary, NC, USA: SAS Institute; 2004.
  19. Rezaei J, Rouzbehan Y, Zahedifar M, Fazaeli H. Effects of dietary substitution of maize silage by amaranth silage on feed intake, digestibility, microbial nitrogen, blood parameters, milk production and nitrogen retention in lactating Holstein cows. Anim Feed Sci Technol 2015;202:32-41. https://doi.org/10.1016/j.anifeedsci.2015.01.016
  20. Paiva PG, Del Valle TA, Jesus EF, et al. Effects of crude glycerin on milk composition, nutrient digestibility and ruminal fermentation of dairy cows fed corn silage-based diets. Anim Feed Sci Technol 2016;212:136-42. https://doi.org/10.1016/j.anifeedsci.2015.12.016
  21. Blanch M, Carro MD, Ranilla MJ, Viso A, Vazuez-Anon A, Bach A. Influence of a mixture of cinnamaldehyde and garlic oil on rumen fermentation, feeding behavior and performance of lactating dairy cows. Anim Feed Sci Technol 2016;219:313-23. https://doi.org/10.1016/j.anifeedsci.2016.07.002
  22. Lv R, Sato M, Elsabagh M, Obtsu T, Sugino T. Effect of fertilization levels and harvesting stages of grass silage on ruminal phytanic acid production in vitro. The 10th Korea-Japan-China Joint Symposium on Rumen Metabolism and Physiology; 2015.
  23. Belanche A, Fuente G, Pinloche E, Newbold CJ, Baleells J. Effect of diet and absence of protozoa on the rumen microbial community and on the representativeness of bacterial fractions used in the determination of microbial protein synthesis. J Anim Sci 2012;90:3924-36. https://doi.org/10.2527/jas.2011-4802
  24. Razzaghi A, Valizadeh R, Naserian AA, Danesh Mesgaran M, Rashidi L. Effects of sucrose and sunflower oil addition to diet of Saanen dairy goats on performance and milk fatty acid profile. Livest Sci 2015;173:14-23. https://doi.org/10.1016/j.livsci.2014.12.002
  25. Dierking RM, Kallenbach RL, Roberts CA. Fatty acid profiles of orchardgrass, tall fescue, perennial ryegrass, and alfalfa. Crop Sci 2010;50:391-402. https://doi.org/10.2135/cropsci2008.12.0741
  26. Radostits OM, Gay CC, Blood DC, Hinchliffe KW. Veterinary medicine. A text book of the diseases of cattle, sheep, goats and horses. 10th ed. London, UK: Saunders Ltd; 2007.
  27. Reist M, Erdin D, von Euw D, et al. Concentrate feeding strategy in lactating dairy cows: Metabolic and endocrine changes with emphasis on leptin. J Dairy Sci 2003;86:1690-706. https://doi.org/10.3168/jds.S0022-0302(03)73755-2
  28. Huyen NT, Wanapat M, Navanukraw C. Effect of Mulberry leaf pellet (MUP) supplementation on rumen fermentation and nutrient digestibility in beef cattle fed on rice strawbased diets. Anim Feed Sci Technol 2012;175:8-15. https://doi.org/10.1016/j.anifeedsci.2012.03.020
  29. West JW, Hill GM, Utley PR. Peanut skins as a feed ingredient for lactating dairy cows. J Dairy Sci 1993;76:590-9. https://doi.org/10.3168/jds.S0022-0302(93)77379-8
  30. Cherdthong A, Wanapat M, Rakwongrit D, et al. Supplementation effect with slow-release urea in feed blocks for Thai beef cattle-nitrogen utilization, blood biochemistry and hematology. Trop Anim Health Prod 2014;46:293-8. https://doi.org/10.1007/s11250-013-0485-1
  31. Ouellet DR, Chiquette J. Effect of dietary metabolizable protein level and live yeasts on ruminal fermentation and nitrogen utilization in lactating dairy cows on a high red clover silage diet. Anim Feed Sci Technol 2016;220:73-82. https://doi.org/10.1016/j.anifeedsci.2016.07.006