• Title/Summary/Keyword: food vehicle

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Subchronic Toxicity of a Combined Preparation of Ticlopidine and Giekgo Biloba Extract Orally Administered to Rats for 30 Days

  • Kim, Sung Y.;Yim, Hye K.;Yoon, Mi Y.;Kim, Sang K.;Lee, Ja Y.;Oh, Soo J.;Kim, Hye S.;Kang, Sung A.;Kim, Young C.
    • Toxicological Research
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    • v.14 no.4
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    • pp.547-555
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    • 1998
  • The subchronic toxicity of a combined preparation of ticlopidine and ginkgo biloba extract (EGb 761) mixed in a ratio of 10: 4 was examined in male and female Sprague-Dawley rats. Rats were treated with the test substance at a dose of 52 mg/kg, 156 mg/kg, or 467 mg/kg intragastrically for 30 consecutive days. Control rats were treated with vehicle only. Each group consisted of 10 rats. No death or abnormal clinical signs were observed throughout the administration period. A transient decrease in body weight gain and food intake was observed in the rats treated with the high dose (467 mg/kg), which was recovered to normal in a week. There were no drug-related differences in urinalysis and hematological results. A significant increase in serum total cholesterol and total protein was observed in both sexes of the rats treated with a dose of 467 mg/kg daily, but all the other values obtained in serum chemistry appeared to be within normal range. A dose dependent increase in liver weight was observed in both male and female rats. Relative kidney weight was also increased in the high dose groups. There was no gross pathological finding at terminal sacrifice. Microscopic histopathological examination did not show any lesion in terms of correlation with administration of the test substance. The results suggest that under the conditions employed in this study no observable effect level (NOEL) of the test substance be 52 mg/kg/day.

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Improving Field Crop Classification Accuracy Using GLCM and SVM with UAV-Acquired Images

  • Seung-Hwan Go;Jong-Hwa Park
    • Korean Journal of Remote Sensing
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    • v.40 no.1
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    • pp.93-101
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    • 2024
  • Accurate field crop classification is essential for various agricultural applications, yet existing methods face challenges due to diverse crop types and complex field conditions. This study aimed to address these issues by combining support vector machine (SVM) models with multi-seasonal unmanned aerial vehicle (UAV) images, texture information extracted from Gray Level Co-occurrence Matrix (GLCM), and RGB spectral data. Twelve high-resolution UAV image captures spanned March-October 2021, while field surveys on three dates provided ground truth data. We focused on data from August (-A), September (-S), and October (-O) images and trained four support vector classifier (SVC) models (SVC-A, SVC-S, SVC-O, SVC-AS) using visual bands and eight GLCM features. Farm maps provided by the Ministry of Agriculture, Food and Rural Affairs proved efficient for open-field crop identification and served as a reference for accuracy comparison. Our analysis showcased the significant impact of hyperparameter tuning (C and gamma) on SVM model performance, requiring careful optimization for each scenario. Importantly, we identified models exhibiting distinct high-accuracy zones, with SVC-O trained on October data achieving the highest overall and individual crop classification accuracy. This success likely stems from its ability to capture distinct texture information from mature crops.Incorporating GLCM features proved highly effective for all models,significantly boosting classification accuracy.Among these features, homogeneity, entropy, and correlation consistently demonstrated the most impactful contribution. However, balancing accuracy with computational efficiency and feature selection remains crucial for practical application. Performance analysis revealed that SVC-O achieved exceptional results in overall and individual crop classification, while soybeans and rice were consistently classified well by all models. Challenges were encountered with cabbage due to its early growth stage and low field cover density. The study demonstrates the potential of utilizing farm maps and GLCM features in conjunction with SVM models for accurate field crop classification. Careful parameter tuning and model selection based on specific scenarios are key for optimizing performance in real-world applications.

Studies on the Genetic Toxicity of Guh Sung Y.L.S.-95 (목초액 (거성 Y.L.S-95)의 유전독성에 관한 연구)

  • Lee Soo-Yong;Li Guang-Yong;Yin Hu-Quan;Jung Eun-Jung;Kim Youn-Su;Lee Hye-Young;Lee Byung-Hoon
    • Journal of Food Hygiene and Safety
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    • v.21 no.2
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    • pp.107-112
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    • 2006
  • Guh Sung Y.L.S-95 (GS95) is a kind of polyacidic solution, which contains acetic acid as a main component. We investigated in the present study tile genetic toxicity of GS95 according to the standard operation procedure from Korean Institute of Toxicology. In the Salmonella typhimurium reverse mutation assay using TA1535, TA1537, TA98 and TA100, GS95 did not induce mutation up to $5,000{\mu}g/plate$. GS95 did not induce chromosome aberration in Chinese hamster lung fibroblast in the concentration range between 1.25 and 5 mg/mL. In the rodent micronucleus assay, the frequency of micronucleated polychromatic erythrocyte in GS95 treated mice were not increased up to 5,000 mg/kg compared to the vehicle treated mice. Taken all these data together, GS95 was proven to be nongenotoxic in the concentration ranges tested.

Anti-inflammatory Effects of Turmeric (Curcuma longa L.) Extract on Acute and Chronic Inflammation Models (만성 및 급성 염증 모델에서 강황추출물의 효과)

  • Anandakumar, Senthilkumar;Joseph, Joshua Allan;Bethapudi, Bharathi;Agarwal, Amit;Jung, Eun-Bong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.4
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    • pp.612-617
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    • 2014
  • Anti-inflammatory effects of turmeric (Curcuma longa L.) rich in polysaccharides, as well as free of curcuminoids and turmerones were investigated in acute and chronic inflammatory models. Activity against the acute phase of inflammation was evaluated in carrageenan-induced paw edema and xylene-induced ear edema models. The results showed that turmeric extract significantly decreased paw edema volume in the first and third hours after carrageenan injection ($P{\leq}0.05$). Turmeric extract at all dose levels also significantly inhibited xylene-induced ear edema formation ($P{\leq}0.05$). Activity against chronic inflammation was also evaluated in cotton pellet-induced granuloma model. Turmeric extract significantly ($P{\leq}0.05$) decreased the weight of granuloma tissue on cotton pellets in a dose-dependent manner when compared to the vehicle control. Thus, the findings of the study suggest that turmeric extract in effective against both acute and chronic inflammation.

Effects of Polygonatum sibiricum rhizome extract on lipid and energy metabolism in high-fat diet-induced obese mice (고지방 식이 유도 비만 마우스 모델에서 황정 추출물의 지방질 및 에너지 대사 관련 유전자에 대한 효능 연구)

  • Jeon, Woo-Jin;Kim, Ji-Young;Oh, Ik-Hoon;Lee, Do-Seop;Shon, Suh-Youn;Seo, Yun-Ji;Yeon, Seung-Woo;Kang, Jae-Hoon
    • Korean Journal of Food Science and Technology
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    • v.49 no.2
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    • pp.192-202
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    • 2017
  • In this study, factors involved in lipid and energy metabolism following treatment with ethanolic extract of the Polygonatum sibiricum rhizome (ID1216) were evaluated in high-fat diet-induced obese mice. ID1216-treated mice showed a significant reduction in weight gain compared to non-treated mice. ID1216 treatment increased the protein levels of AMP-dependent protein kinase, sirtuin1, peroxisome proliferator-activated receptor ${\gamma}$ coactivator 1-${\alpha}$ ($PGC1{\alpha}$), peroxisome proliferator-activated receptor ${\alpha}$ ($PPAR{\alpha}$) and uncoupling proteins in the adipose tissue, liver and muscle compared to vehicle treatment. Analysis of downstream signals of the sirtuin1 $PGC1{\alpha}$-$PPAR{\alpha}$ pathway showed that ID1216 regulates the expression of ${\beta}$-oxidation related genes such as acyl-CoA oxidase, carnitine palmitoyltransferase1, acyl-CoA dehydrogenase and adipocyte protein 2. In addition, ID1216 increased the expression of adipose triglyceride lipase. These results suggest that ID1216 has anti-obesity effects by regulating the genes involved thermogenesis, ${\beta}$-oxidation and lipolysis in a diet-induced obesity model.

Effects of ethanol extract of Polygonatum sibiricum rhizome on obesity-related genes (황정 에탄올 추출물의 비만 조절 유전자에 대한 효과)

  • Jeon, Woo-Jin;Lee, Do-Seop;Shon, Suh-Youn;Seo, Yun-Ji;Yeon, Seung-Woo;Kang, Jae-Hoon
    • Korean Journal of Food Science and Technology
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    • v.48 no.4
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    • pp.384-391
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    • 2016
  • In previous studies, we confirmed that the ethanol extract of Polygonatum sibiricum (ID1216) has anti-obesity effects on high-fat diet-fed mice. To identify the obesity-related genes affected by ID1216, we studied its effects both in vivo and in vitro. In mice, single administration of ID1216 increased the expression of obesity-related genes including sirtuin1 (SIRT1), peroxisome proliferator-activated receptor ${\gamma}$ coactivator $1{\alpha}$ ($PGC1{\alpha}$) and peroxisome proliferator-activated receptor ${\alpha}$ ($PPAR{\alpha}$) compared to that in mice administered the vehicle; their downstream genes (uncoupling proteins, acyl-CoA oxidase, adipocyte protein 2, and hormone-sensitive lipase) were also increased by ID1216. In fully differentiated 3T3-L1 adipocytes, ID1216 showed the same effects on anti-obesity genes as those in the animal model. Based on these results, we propose that ID1216 has anti-obesity effects by regulating the $SIRT1-PGC1{\alpha}-PPAR{\alpha}$ pathway and their downstream genes, thereby controlling energy and lipid metabolisms.

Effect of Phyto-Extract Fermented Mixture (MP119) on the Sexual Functions and on the Toxicities of Cadmium (식물추출복합발효물(MP119)이 성기능에 미치는 영향 및 카드뮴 독성에 대한 효과)

  • Jang, Young-Sun;Jeong, Jong-Moon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.12
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    • pp.1724-1731
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    • 2009
  • This study was aimed to investigate the effect of phyto-extract fermented mixture (MP119) on the male sexual functions. The MP119 was evaluated for anti-impotency and anti-hypertensive effects via ACE (angiotensin converting enzyme) or PDE (phosphodiesterase) inhibition assay. $IC_{50}$ values of MP119 against ACE and PDE were 241.3${\pm}$35.5 ppm and 372.2${\pm}$33.8 ppm, respectively. To investigate the effect of testosterone expression by MP119, we performed cell media test using mouse Leydig-derived TM3 cells. Production of testosterone in TM3 cell was increased by MP119. Also, NO (nitric oxide) production of HUVEC (human umbilical vein endothelial cell) was increased when MP119 was added to the cultures. Forty male ICR mice were divided into 4 groups. MP119 was orally intubated for 7 days to group 1 and 3, and same volume of vehicle to group 2 and 4 as controls. After that, group 3 and 4 were intraperitoneally injected cadmium chloride at a single dose of 2 mg/kg. On the 8th experimental day, weights of testis, epididymis and seminal vesicle, number of sperm, concentrations of serum testosterone and cGMP were determined. The number of sperm, the concentrations of testosterone and cGMP were significantly increased in two experimental groups (group 1, 3). These results suggest that MP119 enhanced the sexual function of male mice, and could protect the sexual organs from the cadmium chloride as one of the endocrine disrupters.

Toxicity of Aristolochiae radix in F344 rats (청목향 Aristolochiae radix에 있어 F344 랫드의 독성)

  • Kim, Choong-Yong;Kim, Yong-Bum;Yang, Byung-Chul;Lee, Jong-Hwa;Chung, Moon-Koo;Yang, Ki-Hwa;Jang, Dong-Deuk;Han, Sang-Seop;Kang, Boo-Hyon
    • Korean Journal of Veterinary Research
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    • v.45 no.1
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    • pp.29-37
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    • 2005
  • 13-week orally repeated dose toxicity was investigated to ascertain the toxic effects of Aristolochiae radix in F344 rats at dose levels of 0, 1 (0.003 AA, aristolochic acid, mg/kg), 5 (0.014 AA mg/kg), 25 (0.068 AA mg/kg), 125 (0.34 AA mg/kg), and 500mg/kg (AA 1.36 mg/kg). No mortalities were found in any of the dose groups including vehicle control groups of both sexes during the study period. Hematologic and serum biochemical examinations revealed no changes related to the test item in any of the dose groups of both sexes. However, gross findings at necropsy implicated thickening of the stomach wall. In histopathological examinations, prominent findings related to the test item treatment were observed in the stomach and urinary bladder. There were squamous cell papilloma, squamous cell hyperplasia, ulceration and erosion observed in the non-glandular stomach. Squamouse cell hyperplasia was observed at dose levels of more than 125 mg/kg in both sexes and squamous cell papilloma was observed at dose level of 500 mg/kg in both sexes. The incidence and severity of these proliferating lesions including squamous cell hyperplasia and squamous cell papilloma increased with dose dependency. Transitional cell hyperplasia was also observed in the urinary bladder at dose levels of more than 25 mg/kg in both sexes and the incidence and severity of the lesion increased with dose dependency. In conclusion, the toxic changes related to the test item treatment were observed in the stomach and urinary bladder, and the no-observed-adverse-effect level (NOAEL) was estimated to be 5 mg/kg/day for both males and females in F344 rats.

Protection and Detoxification Effects of Oriental Herb Extract Mixture on TCDD-Induced Oxidative Stress (TCDD로 유발된 Oxidative Stress에 대한 생약재 추출물의 방어 및 해독효과)

  • Hwang, Jin-Kook;Lee, Kyung-Jin;Yang, Hee-Jin;Park, Ki-Moon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.3
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    • pp.294-301
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    • 2008
  • This study was carried out to investigate the protective effects and detoxification of oriental herb extracts mixture (Saururus chinensis, Taraxacum platycarpum, Ulmus macrocarpa, Glycyrrhiza glabra, Rhynchosia nulubilis) on TCDD-induced oxidative stress. Thirty five male rats were divided into 5 groups: normal control group received saline; vehicle control group received DMSO and acetone; only TCDD-treated group received multiple intraperitoneal injection of TCDD ($100{\mu}g/kg$) and saline; post-treated group of OHEM (400 mg/kg/day) received oral administration for 5 weeks after TCDD treatment; and pre-treated group of OHEM (400 mg/kg/day) received oral administration for 6 weeks from 1 week before TCDD treatment. The elevated serum activities of alanine transaminase (AST), aspartate transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and atherorganic index due to TCDD were significantly decreased by the treatment of OHEM (p<0.05); the pre-treatment of OHEM was especially effective. Hydropic lesions and cytoplasmic vacuolizations in the liver of TCDD-treated rats were inhibited by the treatment of OHEM. Also, OHEM treatment reduced edemas in small intestine villus of TCDD-treated rats. These results suggest that OHEM from various oriental herb extracts might be a useful protective and detoxification agent against TCDD.

Attenuation Effects of Herbal Mixture Extract on Stress and Sleep Disturbance in Rats (구속 스트레스 및 수면장애를 유도한 흰쥐에서 허브 복합추출물에 의한 개선 효과)

  • Jung, An Na;Lee, Bo Kyung;Lee, Doo Yi;Lee, Ji In;Jung, Yi-Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.3
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    • pp.313-320
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    • 2016
  • In the present study, we examined whether or not an herbal mixture extract (HME) has attenuation effects on immobilization stress and sleep disturbance in rats. Immobilization stress was induced by restricting movement using a constraint box for 2 h, and sleep disturbance was induced by exposure to 300 lx of constant light for 24 h and injection of caffeine (1 mg/kg, i.p.). Rats were orally treated with distilled water (vehicle) or HME for 3 weeks at different doses of 10, 30, and 100 mg/kg/d (HME I, II, and III, respectively). In the immobilization model, HME III treatment significantly decreased adrenal gland weight, whereas HME II and III treatment reduced plasma levels of cortisol. HME II and III also reduced the level of IL-6. In the sleep disturbance model, HME II and III meaningfully reduced the plasma level of cortisol, and the increased plasma level of melatonin. HME III significantly increased body weight. HME reduced immobilization stress and ameliorated disturbance in rats. These findings suggest that HME may have beneficial potential for attenuation of sleep disturbance and stress.