• 한국식품영양학회지
• /
• 제10권4호
• /
• pp.491-496
• /
• 1997

Rhizopus japonicus ATCC33959로부터 미생물성 chitosan을 생산하기 위한 최적 조건을 조사하였다. 탄소원으로써 starch와 glucose(1:1)를 3.0%, 질소원으로써 yeast extract를 3.0%, 무기염류로써 ZnSO4를 0.02% 첨가하고 pH를 5.0으로 맞춘 배지에 Rhizopus japonicus ATCC22959의 포자를 접종한 후 3$0^{\circ}C$에서 96시간 배양하는 것이었다. Chitosan 생산을 위한 기본 배지와 최적 배지에 각각 1.25$\times$105개의 포자를 접종하여 배양한 후 chitosan의 생산성을 비교한 결과는 각각 0.08g/200ml와 0.51g/200ml로 약 6.4배정도 수율이 증가하였다.

• 한국축산식품학회지
• /
• 제21권1호
• /
• pp.24-31
• /
• 2001

The effects of addition of activated carbon to diet of broiler on productivity and meat quality of broilers were investigated. 48 broiler raised for six week. The addition level of activated carbon to each group was added 0, 0.6, 0.9 and 1.2%, respectively. During the experimental feeding period, weekly gain and feed intake of treatment fed diets contain 0.6 and 0.9 percent activated carbon were higher compared with those fed on control diet, though effects of diets containing graded levels of activated carbon on the feed efficiency were not found. When broilers were fed activated carbon on crude protein level of birds were higher compared with that of control diet. Also, crude fat of broilers fed diet containing activated carbon were shown to decrease compared with those fed of control diet(p<0.05). The pH from activated carbon diets was rather higher than that of control(p<0.05). The content of VBN and TBARS was not significantly different among all treatments. The heating loss has tend to decrease in activated carbon diet groups(p<0.05). The WHC tend to be increase in activated carbon diet groups(p<0.05). Blood cholesterol was no significantly different.

• 한국식품영양과학회지
• /
• 제26권6호
• /
• pp.1237-1245
• /
• 1997

Interspecific fusants were made from the cells of two strains of Lactobacillus genus, a streptomycin resistant Lactobacillus sp. JC-7 and a kanamycin resistant L. acidophilus 88. The morphological and physiological properties of the fusants were examined by determining bacteriocin productivity, acid-producing activity, ability of carbohydrates utilization and three important enzyme activities. The fusants produced a bacteriocin against indicator strains and fusant No. 1, 4 exhibited a larger inhibition zone compared to that of L. acidophilus 88. $\beta$-Galactosidase, phospho-$\beta$-galactosidase, lipase activities and resistance to NaCl of Lactobacillus sp. JC-7 were better than those of L. acidophilus 88. Fusant No. 3 and 7 exhibited excellent lipase activities. Protease activity and acid productivity of L. acidophilus 88 were better than those of Lactobacillus sp. JC-7. Proteasse activities of all fusants were higher than those of parental strains, and expecially fusant No. 5 and 7 exhibited excellent proteolysis ability.

• BMB Reports
• /
• 제57권1호
• /
• pp.50-59
• /
• 2024

The application of gene engineering in livestock is necessary for various reasons, such as increasing productivity and producing disease resistance and biomedicine models. Overall, gene engineering provides benefits to the agricultural and research aspects, and humans. In particular, productivity can be increased by producing livestock with enhanced growth and improved feed conversion efficiency. In addition, the application of the disease resistance models prevents the spread of infectious diseases, which reduces the need for treatment, such as the use of antibiotics; consequently, it promotes the overall health of the herd and reduces unexpected economic losses. The application of biomedicine could be a valuable tool for understanding specific livestock diseases and improving human welfare through the development and testing of new vaccines, research on human physiology, such as human metabolism or immune response, and research and development of xenotransplantation models. Gene engineering technology has been evolving, from random, time-consuming, and laborious methods to specific, time-saving, convenient, and stable methods. This paper reviews the overall trend of genetic engineering technologies development and their application for efficient production of genetically engineered livestock, and provides examples of technologies approved by the United States (US) Food and Drug Administration (FDA) for application in humans.

• Journal of Microbiology and Biotechnology
• /
• 제10권1호
• /
• pp.69-74
• /
• 2000

The medium for erythritol production by Torula sp. in a 500-ml baffled flask was optimized to be 300 g/I sucrose, 10 g/I yeast extract, 3 g/I $KH_2PO_4$, and 10 mg/I $CuSO_4{\cdot}5H_2O{\;}at{\;}34^{\circ}C$ with initial pH of 5.5. Using this optimal medium, erythritol of 166 g/I was obtained after 140 h of cultivation, corresponding to 55.3% of the erythritol yield from sucrose with a productivity of 1.11 g/I/h. Optimal concentrations of carbbon and nitrogen sources in a fermentor were higher than that in a flask due to the higher oxygen supply of the fermentor. Employing the medium containing 300 g/I or 400 g/I sucrose for the determination of optimal C/N ratio, the C/N ratio was found to be more important than the nitrogen concentration for effective erythritol production, The optimal ratio of yeast extract to sucrose (g/g) was 20. The yield and productivity of erythritol were maximal in the medium containing 400 g/I sucrose and 20 g/I yeast extract. when dissolved oxygen in the culture was increased, the cell mass increased but the erythritol production was manimal in the range of 5 to 10% of dissolved oxygen. Under the optimal the rane of 5 to 10% of dissolved oxygen. Under the optimal culture condition of the fermentor, a final erythritol concentration of 200 gI was obtained after 120 h with a yield of 50% and the productivity was 1.67 g/I/h. The yield was the highest among erythritol-producting microorganisms

• 한국미생물·생명공학회지
• /
• 제38권4호
• /
• pp.385-390
• /
• 2010

청국장의 기호도를 개선하는 발효균으로 분리된 protease 생산균은 Bacillus licheniformis로 확인되었다. Protease 생산을 위한 배지를 최적화하기 위한 탄소원, 질소원, 인, 금속이온의 성분을 변화시키면서 균의 성장과 효소 생산성을 비교하였다. Glucose를 탄소원으로 사용하였을 때는 균의 성장은 정상적으로 일어나지만, protease 생산이 심하게 억제되는 것으로 나타났으며, Potato starch를 탄소원으로 사용하였을 때 효소 생산성이 가장 높았다. 질소원으로는 yeast extact가 효소 생산에 가장 적합하였다. 한편 2가 금속이온중 $Zn^{2+}$, $Cu^{2+}$, $Co^{2+}$을 배지에 첨가하였을 때는 균의 성장이 심하게 저해되었으며, 효소 생산도 되지 않았다. $CaCl_2$를 첨가한 배지에서는 균이 성장과 효소 생산성이 증가되었다. Potato starch(1.5%), yeast extract(1.5%), $CaCl_2$(0.7%), $K_2HPO_4$(0.03%)와 $KH_2PO_4$(0.03%)를 포함하는 것으로 구성된 최적화 배지에서 최대효소 생산성은 800 U/mL로 나타났으며 28시간 이후에는 배양액내 효소활성이 서서히 감소하였다.

• 한국미생물·생명공학회지
• /
• 제42권2호
• /
• pp.99-105
• /
• 2014

가정에서 제조된 된장을 산성조건에서 계대 배양한 후 균체외 mannanase 를 생산하는 Bacillus sp. YB-1401를 분리하였다. 분리균의 당이용능을 비롯한 생화학적 특성은 Brevibacillus laterosporus와 유사도(61.1%)가 가장 높은 반면에 16S rRNA 유전자의 염기서열은 B. amyloliquefaciens와 유사도가 가장 높았다. 분리균 YB-1401의 mannanase 생산성은 mannans에 의해 급격하게 증가하였으며, 특히 곤약(4%)이 첨가된 배지에서 약 265 U/ml로 최대 생산성을 보였다. 분리균의 mannanase는 $55^{\circ}C$와 pH 5.5 반응조건에서 최대활성을 보였으며 pH 3.5-11.0의 범위에서 1시간 방치하였을 때 실활이 거의 일어나지 않았다. 또한 LBG와 guar gum 및 mannooligosaccharides를 mannanase로 분해하였을 때 주된 반응산물로 mannobiose와 mannotriose가 생성되었으며 mannose도 소량 생성되었다.

• 농업과학연구
• /
• 제50권3호
• /
• pp.395-406
• /
• 2023

The large amount of energy required for successful crop production is the main challenge in greenhouse cropping systems. As a response to this challenge a comprehensive evaluation of greenhouse energy consumption was carried out in two structurally similar single-span greenhouses with different thermal curtain positions, with particular attention to energy productivity, specific energy, net energy, and energy ratio. The greenhouses are used for strawberry production. In the R-greenhouse (RGH), the thermal curtain hanged directly at the roof ridge, whereas in the Q-greenhouse (QGH), the thermal curtain was placed 5° from an imaginary vertical axis, from the middle of the roof ridge downwards to the north side of the greenhouse roof. The relevant data were recorded using standard methods. The results indicated that the energy expended in the RGH and QGH systems was 2,186.48 and 2,189.26 MJ/m², respectively. Electricity and nitrogen fertilizer contributed the highest energy input in both greenhouses and in all seasons. The output energy was 3.12 and 3.82 MJ/m², respectively, in RGH and QGH in season I and 4.40 and 4.87 MJ/m² in season II. In terms of energy expended, there was no significant difference between the two greenhouses, nor between the two seasons. These results indicate that greenhouses of the size used in this investigation are not viable in terms of energy productivity, energy-use efficiency, and subsequent economic performance. However, further studies should be conducted to scale-up the information obtained from this investigation.

• 대한지역사회영양학회지
• /
• 제12권2호
• /
• pp.178-188
• /
• 2007

This study was performed to suggest a new efficiency measurement indicator is necessary for evaluating management efficiency of food service operations in contract-managed foodservice companies, to distinguish efficient food service providers and inefficient ones by measuring comparative efficiency among food service operations, and to provide guidance for effective management through showing benchmarking targets for improving inefficient food service providers. The subjects of this study were the 93 B&I foodservice Operations of a domestic contract food service company. The analysis was conducted using CCR model in DEA model. A software, 'Frontier analyst', was used for the analysis. Based on the results derived from comparison of efficiency evaluation classified by providers with use of DEA, it was possible to identify efficient food providers and inefficient providers, and subsequently provide benchmarking guidelines for improvement of the inefficient groups. In analyzing the differences between the results of DEA efficiency evaluation by detailed operation status of food service providers, there was significant difference of efficiency outcomes in terms of contract types, while there was no significant difference in terms of business condition.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제5권2호
• /
• pp.123-129
• /
• 2000

A high cell density culture system for the anchorage dependent CHO cells was developed based on the combination of in removal of ammonium ion and microcarrier culture system, and semi-fed-batch feeding of glucose and glutamine was employed to the developed culture system. The glass bead was selected as an optimum microcarrier in terms of cell growth. An ammonium ion selective zeolite, Phillipsite-Gismondine, was packed in a dialysis menium ion. The semi-fed-batch operation was employer to the novel culture system for the high density cell culture, and the results showed the cell growth was improved by 32% and tPA productivity by 250%.