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Medium Optimization for the Protease Production by Bacillus licheniformis Isolated from Cheongkookjang  

Yoon, Ki-Hong (Department of Food Science & Biotechnology, Woosong University)
Shin, Hye-Young (Department of Food Science & Biotechnology, Woosong University)
Publication Information
Microbiology and Biotechnology Letters / v.38, no.4, 2010 , pp. 385-390 More about this Journal
Abstract
Bacillus licheniformis fermenting soybean product with highest score in consumer acceptance had been isolated from homemade Cheongkookjang. In order to develop the medium composition, effects of ingredients including nitrogen sources, carbon sources, metal ions and phosphate were examined for protease production of the isolate. Potato starch increased the protease productivity, while glucose repressed it. Yeast extract was the most effective nitrogen source for enzyme production. The calcium was found to increase protease activity slightly while cell growth and enzyme production was completely inhibited by divalent ions such as $Zn^{2+}$, $Cu^{2+}$ and $Co^{2+}$. The maximum protease productivity was reached approximately 800 unit/mL in the optimized medium consisting of potato starch (1.5%), yeast extract (1.5%), $CaCl_2$(0.7%), $K_2HPO_4$(0.03%) and $KH_2PO_4$(0.03%). The protease activity of culture filtrate was gradually decreased after incubation for 28 h.
Keywords
Bacillus licheniformis; protease productivity; medium; optimization;
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Times Cited By KSCI : 10  (Citation Analysis)
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