• 한국축산식품학회지
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• 제41권5호
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• pp.802-815
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• 2021

To investigate the effect of postmortem phases on lamb meat quality, the physicochemical quality, microstructure and water mobility of oyster cut, short loin, knuckle and silverside muscles from Small-Tail Han sheep were evaluated in the pre-rigor, rigor mortis and post-rigor phases. Pre-rigor lamb meat had higher pH and water holding capacity (WHC), whereas lower CIE L^*, b^*, hue angle values than rigor mortis and post-rigor meat (p<0.05). The Warner-Bratzler shear force (WBSF) values were higher in rigor mortis short loin and silverside than their pre-rigor and post-rigor counterparts, pre-rigor short loin had lower WBSF value than its post-rigor counterpart (p<0.05). Muscle fibers shrank laterally and longitudinally during the onset of rigor mortis. Rigor mortis and postrigor lamb meat exhibited wide I-bands, dark A-bands, short sarcomeres and large inter-myofibrillar spaces. The shift of immobilized water to free water and repulsion from the intra-myofibrillar space to the extracellular space result in the increase of water loss in rigor mortis and post-rigor lamb meat. The results of the principal component analysis (PCA) indicated that rigor mortis and post-rigor lamb meat had similar quality properties but different from pre-rigor lamb meat. In conclusion, the lamb meat in the pre-rigor phase had good tenderness, color and WHC. The results of this research could provide some theoretical references for lamb meat production and processing.

• 대한수의학회지
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• 제58권4호
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• pp.177-182
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• 2018

Canine adenovirus type 2 (CAV-2) infection results in significant respiratory illness in dogs. Isolating and culturing CAV-2 allows for investigations into its pathogenesis and the development of vaccines and diagnostic assays. In this study, we successfully isolated a virus from a naturally infected dog in Gyeonggi-do, Korea. The virus was propagated in Madin-Darby canine kidney (MDCK) and Vero cells and showed a specific cytopathic morphology that appeared similar to a bunch of grapes. The virus was first confirmed as CAV-2 based on these cytopathic effects, an immunofluorescence assay, hemagglutination assay, and electron microscopy. The viral titer of the isolate designated APQA1601 reached $10^{6.5}$ 50% tissue culture infections dose per mL in MDCK cells and exhibited no hemagglutination units with erythrocytes from guinea pig. The virus was also confirmed by polymerase chain reaction and next-generation sequencing. The APQA1601 strain had the highest similarity (~99.9%) with the Toronto A26/61 strain, which was isolated in Canada in 1976 when the nucleotide sequences of the full genome of the APQA1601 strain were compared with those of other CAV strains. Isolating CAV-2 will help elucidate the biological properties of CAV-2 circulating in Korean dogs.

• 한국축산식품학회지
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• 제42권5호
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• pp.816-832
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• 2022

To evaluate the effects of early postmortem vacuum packaging (VP) on meat quality during postmortem chilled storage, hot-boned lamb was vacuum-packaged at 1, 6, 12, 24, and 48 h postmortem and stored around 2℃ until 168 h postmortem, with lamb packaged in plastic wrap as the control (aerobic packaging). Intramuscular pH decline was delayed when lamb was vacuum packaged at 1, 6, and 12 h postmortem (p<0.05). The lamb vacuum-packaged at 1 h postmortem (VP-1h group) had significantly lower shear force values and purge losses accompanied by lower free thiol group values than other treatments during postmortem storage and was also higher in extractable calpain-1 activity by 6 h postmortem (p<0.05). Free thiol group concentrations were significantly higher after VP at 6 and 12 h postmortem (p<0.05). Packaging lamb under vacuum very early postmortem produced the lowest shear force and purge loss, likely by slowing heat loss and muscle temperature decline, implying that lamb quality is improved by VP when applied very early postmortem. This was at the expense of protein oxidation, which was unrelated to other meat quality measurements, most likely because potential contracture during hot boning confounded its impact. Further research is required to understand the implications of the interaction between protein oxidation, VP, and hot boning on the acceptability of lamb.

• 대한수의학회지
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• 제62권3호
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• pp.19.1-19.6
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• 2022

Japanese encephalitis virus (JEV) and Akabane virus (AKAV) are mosquito-borne viruses that cause encephalitis and reproductive disorders in horses and cattle, respectively. There is no treatment for JEV or AKAV infections in animals. Therefore, we evaluated the antiviral activity of 18-mer amphipathic peptides in the 1b-4/21-C series on JEV and AKAV using Vero cells in vitro and evaluated their effects on JEV in mice. Of 6 peptides, 1b-4/21-C12 had the lowest IC₅₀ of 0.313 against JEV and its use as an antiviral against JEV and AKAV was examined. The IC₅₀ of 1b-4/21-C12 against JEV and AKAV was 0.78 and 1.14 µM, respectively. Mice treated with 5 or 2 mg/kg of 1b-4/21-C12 had 32% and 16% survival rates, respectively, and the surviving mice treated with 1b-4/21-C12 began to gain weight beginning 8 days post challenge with the virulent Nakayama strain. Moreover, 20 µM 1b-4/21-C peptide had no cytotoxic effects on Vero cells. Our in vitro and in vivo results indicate that 1b-4/21-C12 has antiviral activity against enveloped JEV and AKAV and might be useful as a therapeutic substance.

• Journal of Veterinary Science
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• 제21권2호
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• pp.22.1-22.9
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• 2020

Rabid raccoon dogs (Nyctereutes procyonoides koreensis) have been responsible for animal rabies in South Korea since the 1990s. A recombinant rabies vaccine strain, designated as ERAGS, was constructed for use as a bait vaccine. Therefore, new means of differentiating ERAGS from other rabies virus (RABV) strains will be required in biological manufacturing and diagnostic service centers. In this study, we designed two specific primer sets for differentiation between ERAGS and other RABVs based on mutation in the RABV glycoprotein gene. Polymerase chain reaction analysis of the glycoprotein gene revealed two DNA bands of 383 bp and 583 bp in the ERAGS strain but a single DNA band of 383 bp in the field strains. The detection limits of multiplex reverse transcription polymerase chain reaction (RT-PCR) were 80 and 8 FAID₅₀/reaction for the ERAGS and Evelyn-Rokitnicki-Abelseth strains, respectively. No cross-reactions were detected in the non-RABV reference viruses, including canine distemper virus, parvovirus, canine adenovirus type 1 and 2, and parainfluenza virus. The results of multiplex RT-PCR were 100% consistent with those of the fluorescent antibody test. Therefore, one-step multiplex RT-PCR is likely useful for differentiation between RABVs with and those without mutation at position 333 of the RABV glycoprotein gene.

• 한국농공학회논문집
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• 제65권2호
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• pp.13-20
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• 2023

In the past, the Ministry of Agriculture, Food, and Rural Affairs (MAFRA) conducted research into and production of agricultural statistics. As a result of organizational changes, these responsibilities were transferred to Statistics Korea in 1998 and 2008. So, the MAFRA managed data without a formal system for managing data and statistical information. The demand for a foundation to improve the usefulness of agri-food statistics has recently increased due to the necessity for basic data representing the realities of rural and agricultural areas. This is especially true for field-oriented agricultural administration. This study aims to propose a strategy for the systematically integrated administration of dispersed data and statistical information. It served as the foundation for the integrated management of distributed administrative and statistical data.

• 한국농촌건축학회논문집
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• 제24권4호
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• pp.41-48
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• 2022

The purpose of this study is to present the implications of the rural living area setting and rural spatial structure analysis of the rural spatial strategy plan through case analysis. Following the decentralization of autonomy and the promotion of the transfer of state affairs to local areas, some of the general agricultural and fishing village development projects of the Ministry of Agriculture, Food and Rural Affairs were transferred to local areas. In addition, in 2020, the Ministry of Agriculture, Food and Rural Affairs introduced a rural agreement system for 113 cities and counties. However, since the understanding of the field is still low, this study attempted to present implications through a case analysis of the structure of rural space and the establishment of rural living areas in three local governments in Chungcheong. As a result of the study, in the stage of establishing the living zone, it is necessary to comprehensively review the contents reviewed in the analysis of the current status and conditions. However, the two local governments had insufficient interpretation of the axis by function and the establishment of the base area, or were using the upper plan as it was. Second, the source of the data used in the survey on the distribution status of living SOC facilities is unclear. Since the distribution status of living SOC facilities overlaps with accessibility analysis, OD data analysis, and resident survey results and is used as the basis for setting living areas, the data source should be clearly marked, and verification should be possible. Third, amenity (facility) resources were classified and analyzed according to the conditions and characteristics of local governments. The local government that applied the guidelines as they were was A local government, and the remaining local governments conducted surveys and analysis according to the current status. Forth, since the establishment of the rural living area is the minimum unit supported by government policies through future rural agreements, it is necessary to distinguish them based on quantitative standards in the process of establishing them. Only one case area presented clear grounds and standards, and the rest of the case areas presented standards but are based on non-quantitative standards. In order for rural agreements to be sustainable in the future and benefit local residents, it is necessary to use or establish a government-level institution that can collect and produce objective data. In addition, it is necessary to find ways to specify and standardize the standards for setting the living area and specify them in the guidelines.

• 농업과학연구
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• 제47권1호
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• pp.95-104
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• 2020

The Ministry of Agriculture, Food, and Rural Affairs is currently pushing forward policies targeting the Rural Convergence Industry initiative to incubate the front-runners of the '6th industry', to increase off-farm income, to create rural jobs for elderly, and to create women-based farms. As a result, in 2017, a total of 11,604 Rural Convergence Industries were in business, and the goods produced from those industries were diverse. However, the quality and safety of those products are not being checked and managed thoroughly, which has caused consumers to not trust products made from the Rural Convergence Industries. Through a survey and the ordering logit model, this study analyzed consumer perception of the safety and quality of goods from the Rural Convergence Industries. The analysis revealed that we must first raise awareness of the importance of rural agriculture areas and the products of the Rural Convergence Industries to uplift consumer perceptions regarding the products produced by the industries. The analysis also revealed that diverse points of distribution (sellers), education on the importance of rural and urban areas, and marketing Rural Convergence Industry products within suburban consumer populations are necessary to increase the consumption of these products.

• 한국농촌건축학회논문집
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• 제17권1호
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• pp.35-44
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• 2015

This study is regarding Rural village renovation project that improve the poor residential environment of backward rural district in Korea and the quality of life of the rural area residents. Especially, by comparing the Kyungbuk, Yeungju, Juchi village's case which was progressed by MAFRA (Ministry of Agriculture, Food and Rural Affairs) in 2014 for the demonstration project with the successful case MELAP(Model project on the Effective Utilization of Rural Land through the activation of internal potential element) which is one of the Rural area Development Program in German Federal Ministry of Baden-$W{\ddot{u}}rttemberg$, this essay attempts to suggest the improvement Plan of Rural village renovation project which will be developed forward.

• 정보처리학회논문지:소프트웨어 및 데이터공학
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• 제12권3호
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• pp.117-124
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• 2023

농림축산식품부는 2008년 조직개편을 통해 식품 정책에 대한 기능이 이관되어 보건복지부 등에 산재하여 있던 식품산업을 통합관리 하게 되었고, 1, 2, 3차 산업을 포괄한 종합 정책을 수립하고 있다. 최근 4차 산업혁명과 더불어 농축산식품산업에도 스마트팜, 푸드테크 등 새로운 사업개념이 등장하였다. 농림축산식품부는 4차산업에 적합한 정책을 수립하기 위해 농축식품산업의 사업체 규모를 정확히 추정하는 것이 필요하게 되었다. 농림축산식품부는 전방과 후방 산업의 연계분석을 통한 부가가치 산출과 농축산식품산업의 정확한 모집단을 추정하기 위해서 2017년부터 농축산식품산업 특수분류를 위해 연구하기 시작하였고 통계청으로부터 농축산식품 특수분류 승인을 받았다. 특수 분류를 기반으로 한 농축산식품산업의 모집단 추정은 매출액, 종사자 수 등 전 산업에서 차지하는 비중이 정책의 수립과 중요도에 많은 영향을 미치기 때문에 매우 중요하다. 본 논문은 농축산식품 특수분류와 한국표준산업분류에서의 추출된 표본을 이용하여 현재적격률을 산정하고 모집단을 추정하는 과정과 현재적격률을 보완하기 위한 추정적격률을 제안하여 보다 모집단을 잘 반영하는 방안을 제시하고자 한다.