• Asian-Australasian Journal of Animal Sciences
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• 제29권8호
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• pp.1065-1074
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• 2016

Growth factors play an important role during early ovarian development and folliculogenesis, since they regulate the migration of germ cells to the gonadal ridge. They also act on follicle recruitment, proliferation/atresia of granulosa cells and theca, steroidogenesis, oocyte maturation, ovulation and luteinization. Among the growth factors, the growth differentiation factor 9 (GDF9) and the bone morphogenetic protein 15 (BMP15), belong to the transforming growth factor beta (TGF-${\beta}$) superfamily, have been implicated as essential for follicular development. The GDF9 and BMP15 participate in the evolution of the primordial follicle to primary follicle and play an important role in the later stages of follicular development and maturation, increasing the steroidogenic acute regulatory protein expression, plasminogen activator and luteinizing hormone receptor (LHR). These factors are also involved in the interconnections between the oocyte and surrounding cumulus cells, where they regulate absorption of amino acids, glycolysis and biosynthesis of cholesterol cumulus cells. Even though the mode of action has not been fully established, in vitro observations indicate that the factors GDF9 and BMP15 stimulate the growth of ovarian follicles and proliferation of cumulus cells through the induction of mitosis in cells and granulosa and theca expression of genes linked to follicular maturation. Thus, seeking greater understanding of the action of these growth factors on the development of oocytes, the role of GDF9 and BMP15 in ovarian function is summarized in this brief review.

• 대한한방부인과학회지
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• 제23권2호
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• pp.1-19
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• 2010

Purpose: This study was designed to investigate the effects of Gaeullijin-Tang(GIT) on the progression of the estradiol valerate(EV)-induced polycystic ovaries(PCO) in rats. Methods: PCO was induced by single intramuscular injection with estradiol valerate(EV)(4mg) in female rats. Normal group(n=8) were injected with sesame oil and orally administrated distilled water for sixty days. PCO control group (n=8) were injected with EV and orally administrated distilled water for sixty days. GIT treated group(n=8) were injected with EV and orally administrated GIT for same duration. At the end day of experiment, we measured weights of body, ovaries, adrenal glands, uterus and contents of serum androsternedione (ADD) and total estrogen. The histomorphometrical changes of ovaries were also evaluated. And we observed the NGF and CRF expression by immunohistochemistry. Results: The results were as follows - The weights(mg) of ovaries in GIT treated group($75.5{\pm}4.3$) were significantly increased(p<0.001) compared with PCO control group($54.3{\pm}4.5$). - The numbers of mature follicles in GIT treated group($5.3{\pm}1.2$) were significantly increased(p<0.05) compared with PCO control group($3.5{\pm}1.2$). - The numbers of corpora lutea in GIT treated group($1.8{\pm}1.3$) were significantly increased(p<0.05) compared with PCO control group($0.3{\pm}0.7$). Conclusion: From the above results, we concluded that Gaeullijin-Tang(GIT) contributes to stimulating normally ovulation by decreasesing ovarian NGF concentrations.

• 한국수정란이식학회지
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• 제15권2호
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• pp.147-156
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• 2000

To investigate fatty acid constituents and relative compositions in the fluid of the follicles, oviducts, uterine body and uterine body in Korean native cow, the fluids of the reproductive tract were analyzed using gas chromatography. The samples were taken from various reproductive tract of 23 Korean native cows. q. Caprylic acid (C8:0), myristic acid(C14:0), palmitic acid(C16:0), palmitoleic acid(C16:1), stearic acid(C18:0), oleic acid(C18:1), linoleic acid(C18:2), arachidonic acid(C20:4) were found in the reproductive tracts of the cows, which made 8 kinds of fatty acid in total. 2. Palmitic acid, oleic acid, stearic acid were predominant with 35.67%, 24.98% and 17.52%, respectively. while low levels of fatty acids(<5%) were myristic acid, palmitoleic acid and caprylic acid with 1.75%, 1.28% and 2.69%, respectively. 3. Two kinds of polyunsaturated fatty acids, linoleic acid arachidonic acid were found in the reproductive tracts of cows. 4. Palmitic acid among saturated fatty acids and oleic acid among unsaturated fatty acids were the highest level in all of the reproductive tracts. 5. The Highest level of arachidonic acid was found in the uterine horn. 6. The sum of the palmitic acid and oleic acid were 61.72%, 63.72%, 57.66% and 57.65% for the fluid of follicle, oviduct, uterine horn and uterine body of the cows, respectively. 7. The relative compositions of palmitic acid, palmitoleic acid and caprylic acid were higher during the luteal phase than during the follicular phase. 8. The relative compositions of arachidonic acid was higher during the follicular phase in the fluid of uterine horn and uterine body of the cows. 9. The long chain fatty acid, the palmitic acid, stearic acid, oleic acid and linoleic acidshowed higher relative compositions during the follicular phase(86.49%∼95.51%) than during the luteal phase(85.64%∼88.93%).

• Parasites, Hosts and Diseases
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• 제35권2호
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• pp.95-104
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• 1997

서울주걱흡충의 충체 항원의 유래 부위를 알기 위하여 면역전자현미경법으로 관찰하였다. 충체 조항원으로 면역시킨 마우스 혈청을 분리하여 충체 조직과 반응시켰다. 조직 부위 중 조직융해구 세포의 과립세포질세망 저정낭의 정자 사이 조직과 정자머리 주위. 맹장의 미세융모 난황이 면역된 마우스 혈청과 강하게 반응하였다. 감염 마우스 혈청과는 난황 과립이 강하게 반응하고. 맹장의 미세융모가 악하게, 다른 부위는 매우 약하게 반응하였다. 고러므로 조직융해구. 저정낭. 맹장. 난황이 조항원의 유래 부위이고. 충체분비물의 항원은 난황에서 유래하는 것이 가장 강하게 작용하는 것으로 생각한다.

• 한국수의병리학회지
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• 제7권1호
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• pp.63-65
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• 2003

A 10-year-old Korean native female Jin-do dog revealed two growing subcutaneous masses measuring 7.0 ${\times}$ 7.0 ${\times}$ 2.5 cm and 5.0 ${\times}$ 4.0 ${\times}$ 2.0 cm in the left shoulder and lower part of chest, respectively. Grossly tumor masses were well-circumscribed and exhibited ulcerated surface and purulent exudate on the cut surface. Histologically tumor masses were characterized by cystic structures some of which were fused together. Although the cells of cyst wall differentiated to those similar to infundibulum, isthmus, inferior segment of hair follicles, respectively, the most cyst wall consisted of cells similar to infundibulum The luminal content of cysts depended on the cells of cysts; infundibulum-like cyst contained lamellar keratin, isthmus-like cyst was amorphous keratin, and inferior segment-like cyst had shadow cells. From these results, these tumor masses were diagnosed as multiple trichoepithelioma. To our knowledge, this is the first report of canine multiple trichoepthelioma in a dog in Korea.

• 대한소아치과학회지
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• 제35권2호
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• pp.339-344
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• 2008

상악 영구 견치의 매복에 비해 하악 영구 견치의 매복은 매우 드물게 일어난다. 치아가 매복된 경우 장애물 존재 여부와 매복 위치와 방향, 맹출 가능한 공간의 유무, 치근의 형성 단계 등의 요소에 따라 매복 치아의 발거 혹은 장애물의 제거 후 주기적 관찰, 맹출이 지연된 치아의 외과적 노출, 교정적 견인 등의 방법으로 치료하게 된다. 본 증례는 하악골의 골절 부위에 시행된 골간 강선 결찰과 미니 플레이트에 의해 하악 좌측 견치가 매복되어 있었던 경우로 CT 검사 결과 하악 정중부위 플레이트의 스크류가 맹출을 방해하고 있는 것으로 판단되어 전신 마취 하에 플레이트를 제거하였다. 이후 자연적인 맹출을 기대하였으나, 맹출력이 부족하여 교정적 견인으로 양호한 결과를 얻었기에 보고하는 바이다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권4호
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• pp.496-500
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• 2007

Holstein cow ovaries obtained at a slaughterhouse were used to study the influence of the oocyte collection methods (slicing, puncture, aspiration I and II) on recovery efficiency and subsequent in vitro maturation and embryonic development competence of immature oocytes recovered. In the slicing method, the whole ovarian was chopped into small pieces with a surgical blade. In the puncture method, the whole ovarian surface was punctured by 18-g needle. In other 2 aspiration methods, collected oocytes by aspirating from the visible follicles using an 18-g needle attached to a 5 ml syringe (aspiration I) or using a constant negetive pressure (-80 mmHg) with a vacuum pump (aspiration II). The oocytes were classified into 4 classes on the basis of the morphology of cumulus cells and cytoplasmic appearance of oocyte. Slicing ($9.6{\pm}0.4$) and puncture ($9.7{\pm}0.4$)yielded a larger number of oocytes per ovary than other two aspiration methods (aspiration I and II were $5.8{\pm}0.3$and $5.6{\pm}0.4$, respectively) (p<0.05). The number of the highest quality oocytes (grade A) per ovary was significantly higher in slicing ($4.2{\pm}0.2$) and puncture ($4.6{\pm}0.1$) methods than in other methods (aspiration I and II were $1.2{\pm}0.2$ and $1.4{\pm}0.2$, respectively) (p<0.05). The rate of nuclear maturation of the highest and higher quality oocytes (grade A and grade B, respectively) was not affected by the oocytes collection methods. The oocytes collection methods also did not influence subsequent embryonic developmental competence after in vitro fertilization with M II stage oocytes. It is concluded that slicing and puncture methods of the ovaries can be used as an alternative techniques to aspiration by the syringe or vacuum pump.

• Asian-Australasian Journal of Animal Sciences
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• 제33권11호
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• pp.1714-1724
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• 2020

Objective: MicroRNAs (miRNAs) are the most abundant small RNAs. Approximately 2,000 annotated miRNAs genes have been found to be differentially expressed in ovarian follicles during the follicular development (FD). Many miRNAs exert their regulatory effects on the apoptosis of follicular granulosa cells (FGCs) and FD. However, accurate roles and mechanism of miRNAs regulating apoptosis of FGCs remain undetermined. Methods: In this review, we summarized the regulatory role of each miRNA or miRNA cluster on FGCs apoptosis and FD on the bases of 41 academic articles retrieved from PubMed and web of science and other databases. Results: Total of 30 miRNAs and 4 miRNAs clusters in 41 articles were reviewed and summarized in the present article. Twenty nine documents indicated explicitly that 24 miRNAs and miRNAs clusters in 29 articles promoted or induced FGCs apoptosis through their distinctive target genes. The remaining 10 miRNAs and miRNAs of 12 articles inhibited FGCs apoptosis. MiRNAs exerted modulation actions by at least 77 signal pathways during FGCs apoptosis and FD. Conclusion: We concluded that miRNAs or miRNAs clusters could modulate the apoptosis of GCs (including follicular GCs, mural GCs and cumulus cells) by targeting their specific genes. A great majority of miRNAs show a promoting role on apoptosis of FGCs in mammals. But the accurate mechanism of miRNAs and miRNA clusters has not been well understood. It is necessary to ascertain clearly the role and mechanism of each miRNA or miRNA cluster in the future. Understanding precise functions and mechanisms of miRNAs in FGCs apoptosis and FD will be beneficial in developing new diagnostic and treatment strategies for treating infertility and ovarian diseases in humans and animals.

• 한국수산과학회지
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• 제50권1호
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• pp.41-47
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• 2017

Perhaps the most common type of reproductive dysfunction in captive fish is failure of females to undergo final oocyte maturation and thus to ovulate and spawn. The success of aquaculture could therefore be improved by developing techniques to enhance natural spawning, artificial maturation, and/or to induce ovulation in farmed fish. This study aimed to investigate the effects of prostaglandin $E_2$ ($PGE_2$) and prostaglandin $F_{2{\alpha}}$ ($PGF_{2{\alpha}}$) on in vitro oocyte maturation (germinal vesicle breakdown, GVBD) and ovulation in the marine fish Chasmichthys dolichognathus. Post-vitellogenic follicles (0.80-0.94 mm diameter oocytes) were incubated with $PGE_2$ or $PGF_{2{\alpha}}$ at concentrations of 5, 50, or 500 ng/mL for 24 hours. A significant increase in GVBD was seen in 0.84 mm and 0.94 mm oocytes incubated with 50 ng/mL $PGE_2$ compared with the control. There was no significant increase in GVBD in any of the other experimental conditions (5 or 500 ng/mL $PGE_2$ or 5, 50, or 500 ng/mL $PGF_{2{\alpha}}$). Neither of the prostaglandins induced ovulation at the concentrations tested.These results suggest that GVBD was induced by incubation with 50 ng/mL $PGE_2$.

• Asian-Australasian Journal of Animal Sciences
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• 제18권7호
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• pp.949-956
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• 2005

The weekly ultrasound scanner (US) observations of reproductive organs in a commercial dairy herd with the popular stanchion style management were conducted for over 26 months. Based on reproductive records, the following were evaluated: 1) the effect of postpartum period commencement of US monitoring on herd reproductive efficacy, and 2) the effectiveness of a US monitoring-based diagnosis and subsequent treatments of reproductive disorders on postpartum reproductive efficiency. The reproductive parameters of cows, which were subjected to US monitoring between Days 30-40 (Day 0 = day of parturition), Days 41-50, Days 51-60, and above Day 61, were compared. The reproductive parameters of cows diagnosed as having reproductive disorders (RD) with US monitoring before or after the first artificial insemination (AI) were also compared. It was found that the day of commencement of US monitoring in cows diagnosed with and without RD significantly affected the period towards the first AI and the open period. In particular, cystic follicles and anoestrus detected either before or after the first AI significantly affected herd reproductive efficiency. The implementation of US monitoring improved reproductive efficiency by reducing the open period and increasing the number of milking cows in the herd. The results of this field trial indicate that the postpartum reproductive management of dairy cows with the use of the US monitoring system is one strategy to improve reproductive efficiency, especially in a high-yielding dairy herd reared stanchion management style.