• Asian-Australasian Journal of Animal Sciences
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• 제12권4호
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• pp.515-519
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• 1999

With the purpose of determining the influence of sexual partners on the oestrous behaviour and to evaluate the accuracy of predicting the time from implant withdrawal to sexual receptivity following a treatment with Synchromate B (SMB), 15 adult Brahman cows were used in each of three phases. During phase I and n, random pairs of animals were induced to display oestrus one pair after the other at daily intervals, while in phase III, cows were induced alternately, every other day, one cow on the 1st day, two on the 3rd, one on the 5th, two on the 7th until all cows were treated. Sixty six percent of the cows in phases I and II, and 80% in phase III came into oestrous after treatment. The interval between implant withdrawal and, expected and observed oestrous was statistically different in all phases. Clustering of oestrous was evident. Cows displayed sexual receptivity within a. range of -24 to +96; -24 to +72 and -216 to +192 hours after implant withdrawal for the three phases, respectively, with a tendency for cows treated first (within treatments), to delay their oestrus signs and vice versa. In phase III, four cows showed oestrous behaviour with the implant in place. These in spite of not observing pre-ovulatory follicles. Correlation values of 0.99, 0.93 and 0.90 (P<0.05) were found respectively among treatments, between the number of cows coming into oestrus and the number of mounts observed. These findings suggest that there are social and behavioural factors in a herd that may override exogenous synchronisation treatments.

• Asian-Australasian Journal of Animal Sciences
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• 제21권7호
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• pp.972-979
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• 2008

It has been recently shown that expression of the LH receptor (LHR) in cumulus cells is related with FSH-induced meiotic resumption of mouse cumulus enclosed oocytes (CEOs). However, to date, it is still unclear whether LHR expression in cumulus cells plays a key role during FSH-induced oocyte maturation. The purpose of this study was to characterize the functional role of LHRs in cumulus cells. CEOs were isolated from eCG-primed preovulatory follicles and cultured in hypoxanthine (HX) arrested medium. LHR protein expression in cumulus cells was time-dependent increasing during the process of FSH-induced oocyte maturation. While the sense oligodeoxynucleotide (ODN) had no effect, antisense ODN inhibited FSH-induced LHR expression and meiotic resumption. Moreover, this antisense ODN against LHR could inhibit FSH-induced mitogen-activated protein kinase (MAPK) phosphorylation. This study suggested that LHR expression in cumulus cells is involved in FSH-induced oocyte meiotic resumption, which process is possibly regulated by MAPK cascade.

• Asian-Australasian Journal of Animal Sciences
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• 제31권3호
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• pp.316-326
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• 2018

Objective: This study investigated the expression of genes in cashmere goats at different periods of their fetal development. Methods: Bioinformatics analysis was used to evaluate data obtained by transcriptome sequencing of fetus skin samples collected from Inner Mongolia cashmere goats on days 45, 55, and 65 of fetal age. Results: We found that FoxN1, FoxE1, and FoxI3 genes of the Fox gene family were probably involved in the growth and development of the follicle and the formation of hair, which is consistent with previous findings. Real-time quantitative polymerase chain reaction detecting system and Western blot analysis were employed to study the relative differentially expressed genes FoxN1, FoxE1, and FoxI3 in the body skin of cashmere goat fetuses and adult individuals. Conclusion: This study provided new fundamental information for further investigation of the genes related to follicle development and exploration of their roles in hair follicle initiation, growth, and development.

• 대한한방부인과학회지
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• 제30권1호
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• pp.1-15
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• 2017

Objectives: The present study was carried out to investigate the effects of YJT on experimental hypothyroidism induced by sodium perchlorate and methimazolein in mice. Methods: 30 one-month-old C57BL6 mice were decided into 4 groups: 1) normal (n=6), 2) sodium perchlorate and methimazolein-induced hypothyroidism control (n=8), 3) hypothyroidism mice treated with low YJT (n=8), 4) hypothyroidism mice treated with high YJT (n=8). Sodium perchlorate and methimazolein were administered for 4 weeks, YJT (low and high) was administered for 2 weeks after sodium perchlorate and methimazolein were initiated for a total duration of 2 week. The changes were observed : weight of body, T3, T4, TSH, follicular cells in the thyroid tissues, LDL cholesterol, HDL cholesterol, triglyceride and free fatty acid, FBG, AST, ALT and so on. Results: YJT did not affect body weight gain. YJT restored free T4 level decreased by sodium perchlorate and methimazolein and prevented shrinking of follicles and proliferation of follicular cells in the thyroid tissues. In addition, YJT lowered total and LDL cholesterol levels elevated by sodium perchlorate and methimazolein respectively and ameliorated distribution of fat in liver tissues. In addition, the effect on fasting blood glucose (FBG), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were also investigated. Conclusions: These data suggest that YJT can be used to treat woman patients which are accompanied with hypothyroidism relatively safely.

• Archives of Plastic Surgery
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• 제43권6호
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• pp.498-505
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• 2016

Background Algae have traditionally been used for promotion of hair growth. Use of hair regrowth drugs, such as minoxidil, is limited due to side effects. The aim of this study was to examine a mixture of Saccharina japonica and Undaria pinnatifida (L-U mixture) on hair growth and to compare the promoting effect of hair growth by a 3% minoxidil and a L-U mixture. Methods To evaluate the hair growth-promoting activity, saline, 50% ethanol, 3% minoxidil, and the L-U mixture were applied 2 times a day for a total of 14 days on the dorsal skin of C57BL/6 mice after depilation. Analysis was determined by using a high-resolution hair analysis system, real-time polymerase chain reaction, and H&E staining. Results On day 14, the hair growth effect of the L-U mixture was the same as that of the 3% minoxidil treatment. The L-U mixture significantly (P<0.05) stimulated hair growth-promoting genes, as vascular endothelial growth factor (VEGF) and insulin-like growth factor -1. Increase of VEGF was observed in the L-U mixture group compared with minoxidil and the negative control. In contrast, the L-U mixture suppressed the expression of transforming growth factor-${\beta}1$, which is the hair loss-related gene. In histological examination in the L-U mixture and minoxidil groups, the induction of an anagen stage of hair follicles was faster than that of control groups. Conclusions This study provides evidence that the L-U mixture can promote hair growth in mice, similar to the effect from minoxidil, and suggests that there is potential application for hair loss treatments.

• 문화기술의 융합
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• 제4권3호
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• pp.253-259
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• 2018

떫은감 자원은 전통적으로 다방면의 여러 조직학적 질병을 치료하고 모발 성장을 비롯한 다양한 생물학적 활성에 매우 높은 효과가 있음이 알려져 왔다. 본 연구는 대머리 패턴의 검은 쥐 모델에 유전적으로 입증된 떫은감 추출물에 대한 발모 및 탈모방지 효능을 알아보고자 하였다. 표준화된 제형을 사용하여 대조군, 떫은감 추출물(10 mg/mouse/day)과 양성대조군인 미녹시딜(2 %)을 매일 두번씩 모발이 성장할 때까지 매일 등 피부에 일정량씩 도포 투여하여 조사하였다. C57BL/ 6 Black 쥐에서 모발 성장 세대를 통해 모발 성장의 변화 패턴이 관찰되었다. 모발 존재 면적과 모발의 길이는 처리 대조군 마우스 보다 떫은감 처리 마우스 처리구에서 유의하게 증가 하였다 (P>0.001). 또한 조직학적 평가에서도 대조군과 비교하여 떫은감 추출물의 처리구에서 모낭세포의 숫자가 현저하게 증가하였다. 따라서 본 실험 결과를 통하여 떫은감 추출물을 피부 도포시 누드 마우스의 모발 성장을 촉진하고 모발의 존재 영역을 확장시키고 모발 길이를 증가시켜 장기 모발 성장기의 지표가 될 수 있음을 알 수 있었다.

• 대한세포병리학회지
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• 제7권2호
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• pp.122-133
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• 1996

This is a retrospective review of fine-needle aspiration cytology(FNAC) smears of 153 cases of thyroid disease performed during August 1989 to July 1995, which were confirmed histologically following surgical operations. FNAC results showed 63 cases(41.2%) of adenomatous goiter, 45 cases(29.4%) of papillary carcinoma, 29 cases(19.0%) of follicular neoplasm, 4 cases(2.6%) of follicular variant of papillary carcinoma, 4 cases(2.6%) of Hashimoto's thyroiditis, 4 cases(2.6%) of $H\ddot{u}rthle$ cell neoplasm, 2 cases(1.3%) of medullary carcinoma and one case(0.7%) each of subacute thyroiditis and of anaplastic carcinoma. The overall accuracy of cytological diagnosis was 83.7%. These data strongly suggest thyroid FNAC is a reliable preoperative diagnostic tool, but FNAC has been less valuable in the diagnosis of follicular lesions than any other disease of the thyroid. Adenomatous goiter was not infrequently interpreted as follicular neoplasia that requires surgery for diagnostic conformation and vice versa. The following findings are considered to be compatible with follicular neoplasm: 1) microfollicles, 2) nuclear grooving, 3) irregularity of nuclear membrane, and 4) irregular arrangement or crowding of follicular cells in groups. The FNAC criteria of adenomatous goiter are as follows: 1) atrophic follicular cells, 2) presence of macrophages, 3) abundant colloid, and 4) large follicles. It is recommended that aspiration of thyroid lesions in order to analyse with critical clinico-pathological approach and surgery is considered only for nodules that are clinically suspicious or unresponsive to hormone therapy or when a diagnosis of follicular neoplasm is made.

• 한국가축번식학회지
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• 제14권1호
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• pp.23-30
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• 1990

These studies were carried out to investigate the effects of fetal calf serum(FCS), estrous porcine serum(EPS), porcine follicular fluid(PFF), hormone and matured cumulus cell(MCC) on in vitro maturation and fertilization of porcine follicular oocytes. The ovaries and testes were obtianed from slaughtered Landrace sow and boars, respectively. The follicular oocytes surrounded with cumulus cells were recovered by aspirating follicular fluid from the visible follicles of diameter 3~5 mm and the semen were prepared from boar's epididymal cauda. The follicular oocytes were cultured in TCM-199 medium containing hormones, FCS, EPS, PFF and MCC for 48hrs. in a incubator with 5% CO2 in air at 36$^{\circ}C$ and then matured oocytes were again cultured for 18~20 hrs. with $1.5\times$106/ml motile capacitated sperm in the modified Tyroide solution containing 100$\mu\textrm{g}$/ml of heparin. The results obtained in these experiments were summarized as follows : 1. The maturation and fertilization rate of the follicular oocytes, cultured in the TCM-199 medium supplemented with 10% FCS and PMSG＋HCG were 55.6~64.5% and 33.3~37.1%, respectively. 2. The maturation and fertilization rate of the follicular oocytes cultured in the TCM-199 medium supplemented with 20% EPS and PMSG＋HCG were 50.0~55.0% and 30.3~33.3%, respectively. 3. The maturation rate(59.0~64.2%) and fertilization rate(34.8~39.3%) of follicular oocytes cultured in TCM-199 medium supplemented 20% FCS and 50% PFF were higher than those of follicular oocytes cultured in TCM-199 medium supplemented with 5%, 10% and 15% FCS and 10% and 50% PFF. 4. The maturation rate(60.0%) and fertilization rate(40.0%) of follicular oocytes cultured in TCM-199 medium supplemented with 20% FCS and granulosa cell (1$\times$106/ml) were significantly higher than those of fiollicular oocytes cultured in TCM-199 medium supplemented with 5%, 10% and 15% FCS and granulosa cell.

• 한국가축번식학회지
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• 제14권1호
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• pp.84-91
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• 1990

본 실험은 돼지난포란의 체외성숙과 체외수정 효과를 높일 수 있는 방법을 찾기 위하여 시도되었으며 직경 1~2mm와 3~7mm 난포로부터 채란된 난자를 mKRB(-BSA)에 돼지발정혈청(ESS), FCS 또는 투석돼지난포액(DFF)을 첨가한 성숙배양에서 24~48시간, 37$^{\circ}C$에서 배양하였다. 성숙된 난포란은 정소상체 정자와 24시간 배양 후 전핵행성 여부를 조사하였다. 36~48시간 배양에서 50~60%의 난자가 metaphase II에 도달되었고 난포 크기(1~2mm와 3~7mm)간에 체외성숙율의 차이는 없었으나 3~7mm 난포란에서 성숙분열이 다소 빨랐다. 체외성숙배양액에 5% ESS, 15% FCS 및 DFF 첨가시 대조구보다 다소 성숙율이 높았다. 체외수정율(전핵형성)은 5% ESS와 15% FCS 첨가 성숙시킨 난포란과 체내 수정능획득 정자와의 수정에서 각각 높은 경향이 있었다. 따라서 돼지난포란의 체외성숙과 수정에 ESS, FCS 및 투석난포액이 유효한 요인이 됨을 알 수 있다.

• 한국가축번식학회지
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• 제20권4호
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• pp.427-432
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• 1997

These studies were performed to approach the precise pathway inducing the meiotic inhibitory action of hypoxanthine on mouse follicular oocytes and to identify the cause of detrimental effect of hypoxanthine on viability of the oocyte in vitro. In addition, a correlation between the meiotic inhibitory effect and the detrimental effect of hypoxanthine was investigated. Mouse follicular oocytes at germinal vesicle(GV) stage were collected from the ovaries of ICR mice by puncturing the antral follicles with a fine needle, at 48 hours after PMSG injection. Oocytes were cultured in Modified Whittingham's T6 media containing hypoxanthine and several materials that involved in metabolism of hypoxanthine, and the effects of the materials on the actions of hypoxanthine were investigated by observing germinal vesicle breake down (GVBD), 1st polar body (PB) extrusion and viability of the oocytes. Phophodiesterase significantly reduced the meiotic inhibitory effect of dbcAMP but did not influence on the inhibitory effect of hypoxanthine. Allopurinol and 6-MP significantly enhanced the meiotic inhibitory effect of hypoxanthine, but the materials themselves also showed the meiotic inhibitory action like hypoxanthine. Hypoxanthine-guanine phosphoribosyltransferase significantly enhanced the meiotic inhibitory effect of hypoxanthine, on the contrary HGPRT itself promoted meiotic resumption of the oocytes. Catalase did not induce any change in the meiotic inhibitory effect of hypoxanthine, but SOD increased the GVBD rate suppressed by hypoxanthine. The detrimental effect of hypoxanthine on viability of the oocytes was significantly reduced by allopurinol and catalase, but SOD increased the GVBD rate suppressed by hypoxanthine. The detrimental effect of hypoxanthine on viability of the oocytes was significantly reduced by allopurinol and catalase, but SOD did not reduce the deterimental effect of hypoxanthine. In conclusion, the meiotic inhibtory effect of hypoxanthine may be caused by guanyl dervartives converted from hypoxanthine via salvage pathway, and superoxide anion may partially participate in the inhibitory effect of hypoxanthine. The detrimental effect of hypoxanthine on viability of the oocytes be cused by hydrogen peroxide produced during the metabolism of hypoxanthine.