Browse > Article
http://dx.doi.org/10.5713/ajas.2008.70534

An Antisense Oligodeoxynucleotide to the LH Receptor Attenuates FSH-induced Oocyte Maturation in Mice  

Yang, Jiange (State Key Laboratory of Agrobiotechnology, College of Biological Sciences China Agricultural University)
Fu, Maoyong (State Key Laboratory of Agrobiotechnology, College of Biological Sciences China Agricultural University)
Wang, Songbo (State Key Laboratory of Agrobiotechnology, College of Biological Sciences China Agricultural University)
Chen, Xiufen (State Key Laboratory of Agrobiotechnology, College of Biological Sciences China Agricultural University)
Ning, Gang (State Key Laboratory of Agrobiotechnology, College of Biological Sciences China Agricultural University)
Xu, Baoshan (State Key Laboratory of Agrobiotechnology, College of Biological Sciences China Agricultural University)
Ma, Yuzhen (State Key Laboratory of Agrobiotechnology, College of Biological Sciences China Agricultural University)
Zhang, Meijia (State Key Laboratory of Agrobiotechnology, College of Biological Sciences China Agricultural University)
Xia, Guoliang (State Key Laboratory of Agrobiotechnology, College of Biological Sciences China Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.21, no.7, 2008 , pp. 972-979 More about this Journal
Abstract
It has been recently shown that expression of the LH receptor (LHR) in cumulus cells is related with FSH-induced meiotic resumption of mouse cumulus enclosed oocytes (CEOs). However, to date, it is still unclear whether LHR expression in cumulus cells plays a key role during FSH-induced oocyte maturation. The purpose of this study was to characterize the functional role of LHRs in cumulus cells. CEOs were isolated from eCG-primed preovulatory follicles and cultured in hypoxanthine (HX) arrested medium. LHR protein expression in cumulus cells was time-dependent increasing during the process of FSH-induced oocyte maturation. While the sense oligodeoxynucleotide (ODN) had no effect, antisense ODN inhibited FSH-induced LHR expression and meiotic resumption. Moreover, this antisense ODN against LHR could inhibit FSH-induced mitogen-activated protein kinase (MAPK) phosphorylation. This study suggested that LHR expression in cumulus cells is involved in FSH-induced oocyte meiotic resumption, which process is possibly regulated by MAPK cascade.
Keywords
Cumulus-enclosed Oocyte; LH Receptor; Oligodeoxynucleotide; Mouse;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
Times Cited By SCOPUS : 6
연도 인용수 순위
1 Harrington, F. E., F. J. Bex, R. L. Elton and J. B. Roach. 1970. The ovulatory effects of follicle stimulating hormone treated with chymotrypsin in chlorpromazine blocked rats. Acta Endocrinol. (Copenh) 65(2):222-228.
2 Jonassen, J. A., K. Bose and J. S. Richards. 1982. Enhancement and desensitization of hormone-responsive adenylate cyclase in granulosa cells of preantral and antral ovarian follicles: effects of estradiol and follicle-stimulating hormone. Endocrinol. 111(1):74-79.   DOI   ScienceOn
3 Stein, C. A. and Y. C. Cheng. 1993. Antisense oligonucleotides as therapeutic agents is the bullet really magical? Sci. 261(5124):1004-1012.   DOI
4 Chen, L., P. T. Russell and W. J. Larsen. 1994. Sequential effects of follicle-stimulating hormone and luteinizing hormone on mouse cumulus expansion in vitro. Biol. Reprod. 51(2):290-295.   DOI   ScienceOn
5 Yamashita, S., K. Nakamura, Y. Omori, K. Tsunekawa, M. Murakami and T. Minegishi. 2005. Association of human follitropin (FSH) receptor with splicing variant of human lutropin/choriogonadotropin receptor negatively controls the expression of human FSH receptor. Mol. Endocrinol. 19(8):2099-2111.   DOI   ScienceOn
6 Xie, H., G. Xia, A. G. Byskov, C. Y. Andersen, S. Bo and Y. Tao. 2004. Roles of gonadotropins and meiosis-activating sterols in meiotic resumption of cultured follicle-enclosed mouse oocytes. Mol. Cell Endocrinol, 218(1-2):155-163.   DOI   ScienceOn
7 Wang, X. N. and G. S. Greenwald. 1993. Human chorionic gonadotropin or human recombinant follicle-stimulating hormone (FSH)-induced ovulation and subsequent fertilization and early embryo development in hypophysectomized FSHprimed mice. Endocrinol. 132(5):2009-2016.   DOI   ScienceOn
8 White, J. H., A. Wise, M. J. Main, A. Green, N. J. Fraser, G. H. Disney, A. A. Barnes, P. Emson, S. M. Foord and F. H. Marshall. 1998. Heterodimerization is required for the formation of a functional GABA(B) receptor. Nature 396(6712):679-682.   DOI   ScienceOn
9 Vassart, G., L. Pardo and S. Costagliola. 2004. A molecular dissection of the glycoprotein hormone receptors. Trends Biochem. Sci. 29(3):119-126.   DOI   ScienceOn
10 Wagner, R. W. 1994. Gene inhibition using antisense oligodeoxynucleotides. Nature 372(6504):333-335.   DOI   ScienceOn
11 AbdAlla, S., H. Lother, A. el Massiery and U. Quitterer. 2001. Increased AT(1) receptor heterodimers in preeclampsia mediate enhanced angiotensin II responsiveness. Nat. Med. 7(9):1003-1009.   DOI   ScienceOn
12 Byskov, A. G., C. Yding Andersen, A. Hossaini and X. Guoliang. 1997. Cumulus cells of oocyte-cumulus complexes secrete a meiosis-activating substance when stimulated with FSH. Mol. Reprod. Dev. 46(3):296-305.   DOI   ScienceOn
13 Andersen, C. Y., M. Baltsen and A. G. Byskov. 1999. Gonadotropin-induced resumption of oocyte meiosis and meiosis-activating sterols. Curr. Top. Dev. Biol. 41:163-185.   DOI
14 Ascoli, M., F. Fanelli and D. L. Segaloff. 2002. The lutropin/choriogonadotropin receptor, a 2002 perspective. Endocr. Rev. 23(2):141-174.   DOI   ScienceOn
15 Aboul-Fadl, T. 2005. Antisense oligonucleotides: the state of the art. Curr. Med. Chem. 12(19):2193-2214.   DOI   ScienceOn
16 Galway, A. B., P. S. Lapolt, A. Tsafriri, C. M. Dargan, I. Boime and A. J. Hsueh. 1990. Recombinant follicle-stimulating hormone induces ovulation and tissue plasminogen activator expression in hypophysectomized rats. Endocrinol. 127(6):3023-3028.   DOI   ScienceOn
17 Fu, M., X. Chen, J. Yan, L. Lei, S. Jin, J. Yang, X. Song, M. Zhang and G. Xia. 2007. Luteinizing hormone receptors expression in cumulus cells closely related to mouse oocyte meiotic maturation. Front Biosci. 12:1804-1813.   DOI
18 Dias, J. A., B. D. Cohen, B. Lindau-Shepard, C. A. Nechamen, A. J. Peterson and A. Schmidt. 2002. Molecular, structural, and cellular biology of follitropin and follitropin receptor. Vitam Horm 64:249-322.   DOI
19 Fan, Q. R. and W. A. Hendrickson. 2005. Structural biology of glycoprotein hormones and their receptors. Endocrine 26(3):179-188.   DOI   ScienceOn
20 Chul-Wook Kim and Ki-Hwa Chung. 2004. MAP Kinase is activated dring the maturation of porcine oocytes. Asian-Aust. J. Anim. Sci. 17(8):1069-1075.   과학기술학회마을   DOI
21 van Tol, H. T., M. J. van Eijk, C. L. Mummery, R. van den Hurk and M. M. Bevers. 1996. Influence of FSH and hCG on the resumption of meiosis of bovine oocytes surrounded by cumulus cells connected to membrana granulosa. Mol. Reprod. Dev. 45(2):218-224.   DOI   ScienceOn
22 Tsafriri, A., M. E. Lieberman, Y. Koch, S. Bauminger, P. Chobsieng, U. Zor and H. R. Lindner. 1976. Capacity of immunologically purified FSH to stimulate cyclic AMP accumulation and steroidogenesis in Graafian follicles and to induce ovum maturation and ovulation in the rat. Endocrinol. 98(3):655-661.   DOI   ScienceOn
23 Su, Y. Q., G. L. Xia, A. G. Byskov, G. D. Fu and C. R. Yang. 1999. Protein kinase C and intracellular calcium are involved in follicle-stimulating hormone-mediated meiotic resumption of cumulus cell-enclosed porcine oocytes in hypoxanthinesupplemented medium. Mol. Reprod. Dev. 53(1):51-58.   DOI   ScienceOn
24 Nuti, L. C., W. H. McShan and R. K. Meyer. 1974. Effect of ovine FSH and LH on serum steroids and ovulation in hypophysectomized immature female rats. Endocrinol. 95(3):682-689.   DOI   ScienceOn
25 Thomas, D. M. and D. L. Segaloff. 1994. Hormone-binding properties and glycosylation pattern of a recombinant form of the extracellular domain of the luteinizing hormone/chorionic gonadotropin receptor expressed in mammalian cells. Endocrinol. 135(5):1902-1912.   DOI   ScienceOn
26 Su, Y. Q., K. Wigglesworth, F. L. Pendola, M. J. O'Brien and J. J. Eppig. 2002. Mitogen-activated protein kinase activity in cumulus cells is essential for gonadotropin-induced oocyte meiotic resumption and cumulus expansion in the mouse. Endocrinol. 143(6):2221-2232.   DOI   ScienceOn
27 Pakarainen, T., F. P. Zhang, L. Nurmi, M. Poutanen and I. Huhtaniemi. 2005. Knockout of luteinizing hormone receptor abolishes the effects of follicle-stimulating hormone on preovulatory maturation and ovulation of mouse graafian follicles. Mol. Endocrinol. 19(10):2591-2602.   DOI   ScienceOn
28 Opalinska, J. B. and A. M. Gewirtz. 2002. Nucleic-acid therapeutics: basic principles and recent applications. Nat. Rev. Drug Discov. 1(7):503-514.   DOI   ScienceOn
29 Simoni, M., J. Gromoll and E. Nieschlag. 1997. The folliclestimulating hormone receptor: biochemistry, molecular biology, physiology, and pathophysiology. Endocr. Rev. 18(6):739-773.   DOI   ScienceOn
30 Shimada, M., M. Nishibori, N. Isobe, N. Kawano and T. Terada. 2003. Luteinizing hormone receptor formation in cumulus cells surrounding porcine oocytes and its role during meiotic maturation of porcine oocytes. Biol. Reprod. 68(4):1142-1149.   DOI
31 Su, Y. Q., J. M. Denegre, K. Wigglesworth, F. L. Pendola, M. J. O'Brien and J. J. Eppig. 2003. Oocyte-dependent activation of mitogen-activated protein kinase (ERK1/2) in cumulus cells is required for the maturation of the mouse oocyte-cumulus cell complex. Dev. Biol. 263(1):126-138.   DOI   ScienceOn
32 Richards, J. S., T. Jahnsen, L. Hedin, J. Lifka, S. Ratoosh, J. M. Durica and N. B. Goldring. 1987. Ovarian follicular development: from physiology to molecular biology. Recent Prog. Horm. Res. 43:231-276.
33 Richards, J. S. 1994. Hormonal control of gene expression in the ovary. Endocr. Rev. 15(6):725-751.   DOI
34 Pierce, J. G. and T. F. Parsons. 1979. Glycoprotein hormones: similar molecules with different functions. UCLA Forum Med. Sci. 21:99-117.
35 Uilenbroek, J. T. and J. S. Richards. 1979. Ovarian follicular development during the rat estrous cycle: gonadotropin receptors and follicular responsiveness. Biol. Reprod. 20(5):1159-1165.   DOI   ScienceOn
36 Kerkela, R., M. Ilves, S. Pikkarainen, H. Tokola, J. Ronkainen, O. Vuolteenaho, J. Leppaluoto and H. Ruskoaho. 2002. Identification of PKCalpha isoform-specific effects in cardiac myocytes using antisense phosphorothioate oligonucleotides. Mol. Pharmacol. 62(6):1482-1491.   DOI   ScienceOn
37 Jones, K. A., B. Borowsky, J. A. Tamm, D. A. Craig, M. M. Durkin, M. Dai, W. J. Yao, M. Johnson, C. Gunwaldsen, L. Y. Huang, C. Tang, Q. Shen, J. A. Salon, K. Morse, T. Laz, K. E. Smith, D. Nagarathnam, S. A. Noble, T. A. Branchek and C. Gerald. 1998. GABA(B) receptors function as a heteromeric assembly of the subunits GABA(B)R1 and GABA(B)R2. Nature 396(6712):674-679.   DOI   ScienceOn
38 Lavoie, C., J. F. Mercier, A. Salahpour, D. Umapathy, A. Breit, L. R. Villeneuve, W. Z. Zhu, R. P. Xiao, E. G. Lakatta, M. Bouvier and T. E. Hebert. 2002. Beta 1/beta 2-adrenergic receptor heterodimerization regulates beta 2-adrenergic receptor internalization and ERK signaling efficacy. J. Biol. Chem. 277(38):35402-35410.   DOI   ScienceOn
39 Lu, Z., G. Xia, A. G. Byskov and C. Y. Andersen. 2000. Effects of amphotericin B and ketoconazole on mouse oocyte maturation: implications on the role of meiosis-activating sterol. Mol. Cell Endocrinol. 164(1-2):191-196.   DOI   ScienceOn
40 McFarland, K. C., R. Sprengel, H. S. Phillips, M. Kohler, N. Rosemblit, K. Nikolics, D. L. Segaloff and P. H. Seeburg. 1989. Lutropin-choriogonadotropin receptor: an unusual member of the G protein-coupled receptor family. Sci. 245(4917):494-499.   DOI
41 Lezoualc'h, F., I. Seugnet, A. L. Monnier, J. Ghysdael, J. P. Behr and B. A. Demeneix. 1995. Inhibition of neurogenic precursor proliferation by antisense alpha thyroid hormone receptor oligonucleotides. J. Biol. Chem. 270(20):12100-12108.   DOI   ScienceOn
42 LaBarbera, A. R. and R. J. Ryan. 1981. Porcine granulosa cells in suspension culture. I. follicle-stimulating hormone induction of human chorionic gonadotropin-binding sites on cells from small follicles. Endocrinol. 108(4):1561-1570.   DOI   ScienceOn