• Microbiology and Biotechnology Letters
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• v.24 no.6
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• pp.647-651
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• 1996

The development of an enrichment method for the rapid and effective identification of Salmonella spp. in sewage or food was studied. As a growth factor for Salmonella, 10 mM cyclic adenosine monophosphate (cAMP) in trypticase soy broth with 0.6% yeast extract (TSBYE) increased cell number five-folds and 0.6% yeast extract in selenite broth increased cell number ten-folds of control. Bile salts in selenite broth was tested for the selection of S. enteritidis in a mixture with Staphylococcus aureus, Pseudomonas aeruginosa, Lactobacillus plantarum and Escherichia coli. The latter four strains were effectively inhibited at 0.1% bile salt. A two-step culture method was used to enrich Salmonella spp.; a primary-enrichment and secondary- enrichment culture. At a primary-enrichment step, selenite broth with 0.6% yeast extract and 10 mM cAMP was used, and at a secondary-enrichment step, 0.1% bile salt was additionally used. Culture times of a primary- enrichment and a secondary-enrichment step were 8 hr and 6 hr, respectively. In this procedure, cell number increased from 10$^{0.3}$ to 10$^{8.5}$ with inhibition of other strains within 14 hr. In the case of an initial cell concentrarion as low as 10$^{-2}$ cfu/ml, a cell number increased to 10$^{7}$ cfu/ml by using a 10 hr primary-enrichment and 6 hr secondary-enrichment procedure.

• Korean Journal of Bronchoesophagology
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• v.5 no.2
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• pp.182-190
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• 1999

OBJECTIVES : To determine the results of posterior cricoid split with costal cartilage graft in the treatment of moderate and severe subglottic stenosis in adults, and to assess the effect of T-tube as a stent in this surgery. PATIENTS AND METHODS : Four adults with moderate and severe subglottic stenosis who underwent posterior cricoid split with costal cartilage graft from 1994 to 1995. Three patients were male and one was female. All of the patients had acquired subglottic stenosis, 2 of whom had a bilateral vocal folds paralysis. The surgical procedure we used included a cricoid lamina split with costal cartilage grafting and T-tube stenting. Arytenoidectomy was a added in 2 patients with bilateral vocal folds paralysis. RESULTS : Three of the 4 patients underwent decannulation, and 1 is still undergoing treatment. 3 patients who underwent decannulation demonstrated marked improvement in their symptoms of airway obstruction and good postoperative voice quality. CONCLUSIONS : The posterior cricoidotomy lumen augmentation with costal cartilage grafting is a safe and effective technique for the treatment of moderate and severe subglottic stenosis in adults and T-tube is a good alternative stenting material for this procedure.

• Macromolecular Research
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• v.10 no.2
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• pp.122-126
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• 2002

A molecularly imprinted polymer (MIP) having both amidine and imidazole functional groups in the active site has been prepared using p-nitrophenyl phosphate as a transition state analogue (TSA). The imprinted polymer MIP with amidine and imidazole found to have the highest hydrolysis activity compared with other MIPs with either amidine or imidazole groups only. It is postulated a cooperative effect between amidine and imidazole in the hydrolysis of p-nitrophenyl methyl carbonate (NPMC) as a substrate when both groups were arranged in proximity by molecular imprinting. The rate enhancement of the hydrolysis by MIP was 60 folds over the uncatalyzed solution reaction and two folds compared with the control non-imprinted polymer CPI having both functional groups. The enzyme-mimetic catalytic hydrolysis of p-nitrophenyl acetate by MIP was evaluated in buffer at pH 7.0 with $K_{m}$ of 1.06 mM and $k_{cat}$ of 0.137 $h^{-1}$ . . .

• Food Science and Preservation
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• v.7 no.2
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• pp.177-183
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• 2000

A supercritical fluid extraction was performed for the extraction of phenolics from grape seeds which up to now have been discarded. The optimum condition for extraction process was predicted through response surface methodology using central composit experimental design. The extraction amount of grape seed phenolics was increased by increasing extraction temperature, pressure, and concentration of co-solvent (ethanol). The optimum extraction conditions were 84.83$^{\circ}$C, 51.50MPa and 1.27% ethanol. The yield of phenolics using SFE was higher with 3 folds than ethanol and 4 folds than hexane but less than 80% methanol. In the respects of food poisoning, the approved solvents were restricted to ethanol and hexane. So, SFE for extraction of phenolics could be powerful alternative method for solvent extraction.

• Speech Sciences
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• v.15 no.3
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• pp.145-156
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• 2008

The goal of this study was to investigate acoustic and physiologic characteristics of two phonation types of 'Khoomei' which is a traditional singing style of people who live around the Altai mountains or Mongolia region. It can be produced two pitches simultaneously - high melody pitch can be perceived along with a low drone pitch. Sygyt and kargyraa styles are the most popular and identifiable styles and they can be recognized as the different sounds depending on the method of voice production. Two trained Mongolians participated and have used at least 5 - 6 years. The characteristics of this voice production were measured by using flexible fiberscope, Stroboscopy, Lx Speech studio, Spead, and Doctor Speech. In Sygyt style, very high vocal fold closure (71.50%) with both true and false vocal folds contact and strong breathing support was observed. They also showed that tongue height and harmonics were increased (around 10dB) with resonance cavity movement. In contrast, it was found that Kargyraa sound had very low pitch with relaxed stomach, less laryngeal tension and lower vocal fold contact (69.50%) than hard Sygyt style sound without raising the tongue during phonation. 'Khoomei' phonation can be made by strong contact of both true and false vocal folds and by increasing the harmonics as well.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.11 no.1
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• pp.69-75
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• 2000

Background and Objectives : The Voice Range Profile(VRP) is a two-dimensional graphic dysplay of an individual's amplitude range as a function of total fundamental frequency range. It is designed as a maximum performance test which can be used as a general indicator of voice problems in the non-professional voice and as a sensitive indicator of problems with the professional voice. The purpose of the study is to obtain a baseline VRT for the classical professional singers and compare it with the normal nonsinger's profile. We also compared the difference of VRP between the classical professional singers who have normal vocal fold and who have vocal folds lesions without dysphonia. Materials and Methods : The VRPs were elicited. from 42 trained classical singers(Soprano 26, Mesosoprano 5, Tenor 9, Bariton 2) and 20 untrained nonsingers(female 10, male 10) using Voice Range Profile Model 4326(Kay Elemetrics USA). The mean values for phonational range with highest and lowest pitch level and range of voice intensity with maximum and minimum intensity level were compared between classical singers and nonsingers. Results and Conclusions : The frequency range and dynamic range were significantly increased for the classical singers in comparison to the nonsingers. But there was no significant difference were found for the VRP between the parts in the classical singers. The classical singers who have vocal fold lesions showed slightly decreased VRP compared to those with healthy vocal folds.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.11 no.1
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• pp.20-27
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• 2000

To determine what is the change of pre and postoperative voice and image analysis parameters and correlations between them, videostroboscopy was analyzed in each 18 patients with unilateral vocal cord paralyses or vocal polyps before and after the surgery from November, 1996 to April, 1999. The correlation between acoustic and aerodynamic parameters was investigated. The software-Videolink and $\pi$-View(Mediface Co, Seoul, Korea)-was used in a quantitative analysis. In unilateral vocal cord paralysis, the glottic angle is well correlated with maximum phonation time, jitter and shimmer preoperatively. The postoperative glottic angle is also correlated with preoperative maximum phonation time. In patients with the vocal polyp, the chink is postoperatively decreased, but the size of the chink and the polyp is not correlated with pre and postoperative voice analysis parameters. These findings reveal that glottic an and vocal fold angle are good indicators of e postoperative glottic configuration in unilateral vocal cord paralysis. Vocal fold ratio is also a useful indicator that represents the length of vocal folds. We consider that the computerized analysis through videostroboscopy is one of objective diagnostic methods in many voice disorders if we can measure a distance between the telelaryngoscope and vocal folds.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.24 no.2
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• pp.83-87
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• 2013

The term "phonosurgery," coined in the early 1960s, refers to surgical procedures that maintain, restore, or enhance the human voice. Phonosurgery includes phonomicrosurgery (endoscopic microsurgery of the vocal folds), laryngoplastic phonosurgery (open-neck surgery that restructures the cartilaginous framework of the larynx and the soft tissues), laryngeal injection (injection of medications as well as synthetic and organic biologic substances), and reinnervation of the larynx. Phonomicrosurgery is a means of maximally preserving the layered microstructure of the vocal fold, that is, the epithelium and lamina propria. The purpose of the surgery is usually to improve the vibratory characteristics of the layered microstructure of the vocal folds. Phonomicrosurgery has developed from convergence of microlaryngoscopic surgical technique theory and the mucosal wave theory of laryngeal sound production. Improvements in technology (i.e., laryngoscopes, handled instruments, and lasers), which in part arise from developments in more frequently performed minimally invasive surgical procedures, will probably facilitate the next generation of procedural innovations. The best methods of optimizing phonosurgical outcomes include making an accurate diagnosis, completing a comprehensive voice evaluation, providing sufficient preoperative therapy, carefully selecting patients to undergo phonomicrosurgical procedures, and requiring sufficient postoperative rest and therapy. Phonomicrosurgery will continue to evolve as a result of the interdependent collaboration of surgeons with voice scientists, speech pathologist, and other voice professionals.

• Journal of the Korean Society of Safety
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• v.30 no.6
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• pp.12-17
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• 2015

In this study, the collapse characteristic of CFRP/Al compound square tube was investigated experimentally. The conclusions are as follows; The impact collapse characteristic of CFRP/Al compound square tube was found to be the most superior stacking conditions $[90^{\circ}]_8$. It showed that a very stable collapse mode was crushing. In the member with $[0_2{^{\circ}}/90_2{^{\circ}}]_s$ and $[90_2{^{\circ}}/0_2{^{\circ}}]_s$, stacking conditions, $0^{\circ}$ fibers were splayed to the external by laminar bending, while the $90^{\circ}$ fibers were held between the folds of the aluminum member by laminar bending, local buckling and transverse crack. In the member with $[45_2{^{\circ}}/45_2{^{\circ}}]_s$ stacking conditions, fibers were held between the folds of the aluminum member by local buckling and transverse crack.

• Applied Microscopy
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• v.27 no.1
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• pp.1-12
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• 1997

Dorsal and ventral epithelium of leech, Whitemenia edentula, were observed, using both light and electron-microscope. Results are as follows. Epithelia are composed of simple columnar or irregularly-shaped epithelium, the transveral folds were seen in the cross-sectioned dorsal and ventral epithelia, but only longitudinal folds(depth $500{\mu}m$, width $350{\mu}m$) were discovered in the ventral epithelium. As to muscular tissue under the dorsal epithelium of Whitemenia edentula, that of longitudinal muscle layer is thicker and more developed than that of circular muscle layer. Seven gland cells (type-A, B, C, D, E, F and H cells) and one pigment cell (type-G cell) were discovered in the whitemenia edentula. Those were identified as three kinds of the neutral mucopolysaccharide granoles (type-A, B and F cells), two kinds of acidic mucopolysaccharide granules (type-C and D cells), and two kinds of non-reactive granules respectively (type-E and F cells). The glanules of the type-A and type-B gland cells ae electronly high-dense, and surrounded by the muscular tissue. As to the distribution of gland cells, the type-A, type-B, type-C, type-D, type-I and type-H gland cells were discovered only between the connective tissue and the circular muscle layer, while type-F gland cells were discovered only between tile circular muscle layer and the longitudinal muscle layer.