• Biomolecules & Therapeutics
• /
• 제25권3호
• /
• pp.315-320
• /
• 2017

We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5-FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells.

• 한국해양학회지:바다
• /
• 제7권4호
• /
• pp.247-256
• /
• 2002

평택화력발전소 취수구 주변 해역에서 세립질퇴적물의 운반양상을 규명하기 위하여 표층퇴적물과 부유퇴적물에 대한 모니터링을 실시하였다. 표층퇴적물은 동계에는 상대적으로 개방된 북측해역에서 조립하고 남측해역에서 세립한 반면. 하계에는 조석의 영향에 따라 서측해역에서 조립하고 동측해역에서 세립한 퇴적상의 변화를 보인다. 부유퇴적물의 농도는 동계에 높고 하계에 낮았으며, 표층수보다는 중.저층수에서 높은 농도를 보였다. 또한 조석에 따라서는, 조류의 비대칭성에 따라 육지 방향으로 이동되는 양이 바다 방향으로 이동되는 양에 비해 뚜렷하게 많았으며, 낙조에서 창조로 바뀌는 수심이 낮은 창조 초반에 급격히 증가하는 양상을 보였다. 장.단기간에 걸쳐 관측된 부유퇴적물의 순이동률은 3.4$\times$$10^{-3}$ kg.m$^{-2}$ .s$^{-1}$～5.7$\times$$10^{-3}$ kg.m$^{-2}$ .s$^{-1}$범위로 육지쪽 취수구 방향으로 이동되었다. 이와 같은 현상은 취수구 주변에서 육지쪽으로 발생되는 인위적인 흐름과 주변의 인공구조물에 의해 조류의 순환양상이 변화되었기 때문으로 해석된다.

• 한국수자원학회:학술대회논문집
• /
• 한국수자원학회 2018년도 학술발표회
• /
• pp.291-291
• /
• 2018

하천 및 수자원의 효율적인 관리와 더불어 다양한 수공구조물의 운영 및 관리를 위해서 구조물 주변에서 발생하는 불연속 흐름 및 급변류 등의 현상과 구조물 운영을 반영한 수치해석 기법을 이용한 모델 개발의 중요성이 커지고 있다. 본 연구에서는 하천의 불연속 흐름을 모의하기 위한 1차원 흐름해석 모형(K-River)을 개발하였다. 본 모형은 천이류와 급변류를 수치적으로 안정하게 처리하기 위하여 지배방정식을 보존형 Saint-Venant 방정식으로 선정하고, FVM과 Forward Euler 방법을 이용하여 이산화를 수행하였다. 수치흐름률을 계산하기 위해서 불규칙 단면과 하상의 급경사 등에 신뢰도가 높은 기법으로 판단되는 근사 Riemann해법 중 하나인 HLL flux를 이용하였다. 개발된 K-river 모형의 검증을 위해서 해석해가 존재하는 타원형의 하상융기가 있는 하도에 적용하였으며, 국내에서 하천 설계 및 관리를 위해서 광범위하게 이용되고 있는 1차원 흐름해석 모형인 FDM기반의 HEC-RAS 모의결과와 비교 검토를 수행하였다. 그 결과, FDM기법에서는 모의되지 않는 일부 급변류 패턴을 개발 모형을 통해 모의가능하였으며, 전체적으로 K-River가 기존 모형 보다 해석해에 근사한 결과를 나타내었다. 또한, 배수문을 비롯하여 합류부, 분류부, 펌프장, 암거 등이 설치되어 운영되고 있는 아라뱃길에 적용하여 K-River의 적용성을 평가하였다. K-River를 이용하여 아라뱃길의 흐름분석을 수행한 경우가 HEC-RAS를 이용한 경우보다 수위와 유량의 유동을 시간에 따라 세밀하게 모의하였으며, 이는 배수효과에 의한 파의 전달이 FDM기법 대비 정확히 모의되기 때문으로 판단된다. 추후 연구에서는 현재보다 시간간격을 상세화 하여 수집된 관측수위를 통해 추가적인 검증을 수행하고, 다양한 특성을 가진 타 하천 등에 적용하여 모형의 적용성을 확대하고자 한다.

• Membrane and Water Treatment
• /
• 제1권4호
• /
• pp.297-316
• /
• 2010

Membrane fouling is one of the major operational concerns of membrane processes which results in loss of productivity. This paper investigates the ultrafiltration (UF) results of synthetic oil-in-water (o/w) emulsion using flat sheets of polysulfone (PSf) membrane synthesized with four different compositions. The aim is to identify the mechanisms responsible for the observed permeate flux reduction with time for different PSf membranes. The experiments were carried out at four transmembrane pressures i.e., 68.9 kPa, 103.4 kPa, 137.9 kPa and 172.4 kPa. Three initial oil concentrations i.e., 75 $mgL^{-1}$, 100 $mgL^{-1}$ and 200 $mgL^{-1}$ were considered. The resistance-in-series (RIS) model was applied to interpret the data and on that basis, the individual resistances were evaluated. The significances of these resistances were studied in relation to parameters, namely, transmembrane pressure and initial oil concentration. The total resistance to permeate flow is found to increase with increase in both transmembrane pressure and initial oil concentration while for higher oil concentration, resistance due to concentration polarization is found to be the prevailing resistance. The applicability of the constant pressure filtration models to the experimental data was also tested to explain the blocking process. The study shows that intermediate pore blocking is the dominant mechanism at the initial period of UF while in the later period, the fouling process is found to approach cake filtration like mechanism. However, the duration of pore blocking mechanism is different for different membranes depending on their morphological and permeation properties.

• 대한조선학회 특별논문집
• /
• 대한조선학회 2015년도 특별논문집
• /
• pp.50-55
• /
• 2015

Application of newly conceptualized Anti-Splash Devices designed for COT vent pipes were studied on a P/V valve located on the upper deck of an oil carrier vessel. Anti-Splash devices are used in the shipbuilding industry in order to avoid oil overflow and spray accidents caused by excess pressure and vacuum condition in the cargo oil tanks. These conditions are caused by the transverse and longitudinal sloshing forces that arise from ship motion during sea voyages. The main issue with existing Anti-Splash device model is flux at the outlet of the Anti-Splash Device, and so, new conceptual models for the Anti-Splash device were developed and compared to existing Anti-Splash device model using CFD analysis. Transient analysis was used to capture the flow and velocity of each model and a comparative analysis was performed between old and new-concept models. This data was used to determine the optimal design parameters in order to develop an optimized Anti-Splash Device. A Factory acceptance test was performed on the new-concept models in order to verify the performance and efficiency against their design requirements and other criterion. The final step performed was to apply the optimized Anti-Splash Device models for COT vent pipes to an actual vessel and verify performance through a seawater cargo operation during a sea voyage as per the ship owner's request. The patent for the aforementioned device was obtained by the Korean Intellectual property Office dated Dec. 18th,2014.

• 설비공학논문집
• /
• 제12권1호
• /
• pp.50-58
• /
• 2000

Experiments were carried out to obtain the effects of nozzle configuration and jet to jet spacing on the heat transfer characteristics of single line of circular water jets impinging on a constant heat flux plane surface. The nozzle configurations are Cone type, Reverse cone type and Vertical circular type, and the nozzle arrays are single jet(nozzle dia. 8 mm), 1 row of 3 jets and 1 row of 5 jets. Jet velocities ranging from 3m/s to 8m/s were investigated for the nozzle to target plate spacing of 80 mm. For the Cone and Reverse cone type nozzle arrays, the average Nusselt number of 1 row of 5 jets was larger than that of 1 row of 3 jets at Re$_{D}$<45000, but that of 1 row of 3 jets was larger than that of 1 row of 5 jets at $Reo\le45000$. For the Vertical circular type nozzle, however, the average Nusselt number of 1 row of 3 jets was larger than that of 1 row of 5 jets at all jet velocities. In the condition of fixed mass flow rates, the maximum heat transfer augmentation was obtained for 1 row of 5 jets and was over 2 times larger than that of the single jet for all nozzle configurations. The nozzle configurations that produce the maximum average Nusselt number are as follows: For 1 row of 3 jets, the Vertical circular type at $Reo\le45000$ and the Reverse cone type at $Reo\le45000$. But, they are the Reverse cone type at Re$_{D}$<55000 and the Vertical circular type at$Reo\le55000$ for 1 row of 5 jets.

• 한국기후변화학회지
• /
• 제7권1호
• /
• pp.49-57
• /
• 2016

In the present study, the time series of the number of days on which temperatures were not higher than $-5^{\circ}C$ in winter in Seoul was analyzed. The results showed a decreasing tendency until recently. Statistical change-point analysis was conducted to examine whether climate regime shifts existed in this time series. According to the results, the number of days on which temperatures were not higher than $-5^{\circ}C$ in winter in Seoul drastically decreased since 1988. Therefore, to find out the reason for the recent decrease in the number of days, differences between the means of large-scale environments in winder during 1988~2010 and those during 1974~1987 were analyzed. In all layers of the troposphere, anomalous anticyclones developed in regions around the Korean Peninsula and thus the Korean Peninsula was affected by westerlies or south-westerlies. This was associated with the recent a little further northward development of western North Pacific subtropical high. Therefore, environments good for warm and humid air to flow into the Korean Peninsula were formed. To examine whether relatively warm and humid air actually flowed into the Korean Peninsula recently, temperatures and specific humidity in all layers in the troposphere were analyzed and according to the results the Korean Peninsula showed warm and humid anomalies. In the analyses of sensible heat net flux and maximum temperatures at a height of 2 m that can be felt by humans, the East Asia Continent including the Korean Peninsula showed positive anomalies.

• 한국화재소방학회논문지
• /
• 제33권1호
• /
• pp.50-59
• /
• 2019

과환기 화재조건에서 화원과 측벽의 거리에 따른 화재특성에 관한 실험 및 수치해석 연구가 수행되었다. 1/3 축소된 ISO 9705 화재실이 제작되었으며, Spruce wood crib이 연료로 사용되었다. 구획 내부의 현상 이해를 위하여 Fire Dynamics Simulator (FDS)를 활용한 시뮬레이션이 수행되었다. 개방된 공간 화재에 비해 구획실 화재는 벽면의 열 피드백 효과로 인하여 질량 감소율과 열발생률이 증가됨이 확인되었다. 측벽과 화원의 거리가 감소됨에 따라 화염으로의 공기 유입 제한에도 불구하고 주요 화재특성인 최대 질량 감소율, 열발생률, 화재 성장률, 온도 및 열유속이 증가되었다. 특히 측벽과 화원이 접촉되었을 때 이들 물리량의 가장 큰 변화가 확인되었다. 추가로 화원과 측벽의 거리에 따른 구획 내부의 유동구조의 변화로 인하여 온도의 수직분포에 상당한 변화가 발생됨이 확인되었다.

• Tuberculosis and Respiratory Diseases
• /
• 제85권2호
• /
• pp.147-154
• /
• 2022

Background: The expression of calcium signaling pathway molecules is altered in various carcinomas, which are related to the proliferation and altered characteristics of cancer cells. However, changes in calcium signaling in anti-cancer drug-resistant cells (bearing a T790M mutation in epidermal growth factor receptor [EGFR]) remain unclear. Methods: Afatinib-mediated changes in the level of store-operated Ca²⁺ entry (SOCE)-related proteins and intracellular Ca²⁺ level in non-small cell lung cancer cells with T790M mutation in the EGFR gene were analyzed using western blot and ratiometric assays, respectively. Afatinib-mediated autophagic flux was evaluated by measuring the cleavage of LC3B-II. Flow cytometry and cell proliferation assays were conducted to assess cell apoptosis and proliferation. Results: The levels of SOCE-mediating proteins (ORAI calcium release-activated calcium modulator 1 [ORAI1], stromal interaction molecule 1 [STIM1], and sarco/endoplasmic reticulum Ca²⁺ ATPase [SERCA2]) decreased after afatinib treatment in non-small cell lung cancer cells, whereas the levels of SOCE-related proteins did not change in gefitinib-resistant non-small cell lung cancer cells (PC-9/GR; bearing a T790M mutation in EGFR). Notably, the expression level of SOCE-related proteins in PC-9/GR cells was reduced also responding to afatinib in the absence of extracellular Ca²⁺. Moreover, extracellular Ca²⁺ influx through the SOCE was significantly reduced in PC-9 cells pre-treated with afatinib than in the control group. Additionally, afatinib was found to decrease the level of SOCE-related proteins through autophagic degradation, and the proliferation of PC-9GR cells was significantly inhibited by a lack of extracellular Ca²⁺. Conclusion: Extracellular Ca²⁺ plays important role in afatinib-mediated autophagic degradation of SOCE-related proteins in cells with T790M mutation in the EGFR gene and extracellular Ca²⁺ is essential for determining anti-cancer drug efficacy.

• Nuclear Engineering and Technology
• /
• 제53권12호
• /
• pp.3879-3891
• /
• 2021

A heat pipe residual heat removal system is proposed to be incorporated into the reactor driven subcritical (RDS) facility, which has been proposed by MIT Nuclear Reactor Laboratory for testing and demonstrating the Fluoride-salt-cooled High-temperature Reactor (FHR). It aims to reduce the risk of the system operation after the shutdown of the facility. One of the main components of the system is an air-cooled heat pipe heat exchanger. The alkali-metal high-temperature heat pipe was designed to meet the operation temperature and residual heat removal requirement of the facility. The heat pipe model developed in the previous work was adopted to simulate the designed heat pipe and assess the heat transport capability. 3D numerical simulation of the subcritical facility active zone was performed by the commercial CFD software STAR CCM + to investigate the operation characteristics of this proposed system. The thermal resistance network of the heat pipe was built and incorporated into the CFD model. The nominal condition, partial loss of air flow accident and partial heat pipe failure accident were simulated and analyzed. The results show that the residual heat removal system can provide sufficient cooling of the subcritical facility with a remarkable safety margin. The heat pipe can work under the recommended operation temperature range and the heat flux is below all thermal limits. The facility peak temperature is also lower than the safety limits.