• Title/Summary/Keyword: fluorescence index

Search Result 142, Processing Time 0.031 seconds

Removal characteristics of organic matter during pretreatment for membrane-based food processing wastewater reclamation

  • Jang, Haenam;Lee, Wontae
    • Membrane and Water Treatment
    • /
    • v.9 no.4
    • /
    • pp.205-210
    • /
    • 2018
  • In this study, we investigated coagulants such as polyaluminum chloride (PACl) and ferric chloride ($FeCl_3$) and the combination of a coagulant and powdered activated carbon (PAC) for the removal of dissolved organic matter (DOM) from fish processing effluent to reduce membrane fouling in microfiltration. The efficiency of each pretreatment was investigated through analyses of dissolved organic carbon (DOC) and ultraviolet absorbance at 254 nm ($UVA_{254}$). Membrane flux and silt density index (SDI) analyses were performed to evaluate membrane fouling; molecular weight distributions (MWD) and fluorescence excitation-emission matrix (FEEM) spectroscopy were analyzed to assess DOM characteristics. The results demonstrated that $FeCl_3$ exhibited higher DOC and $UVA_{254}$ removals than PACl for food processing effluent and a combination of $FeCl_3$ and PAC provided comparatively better results than simple $FeCl_3$ coagulation for the removal of DOM from fish processing effluent. This study suggests that membrane fouling could be minimized by proper pretreatment of food processing effluent using a combination of coagulation ($FeCl_3$) and adsorption (PAC). Analyses of MWD and FEEM revealed that the combination of $FeCl_3$ and PAC was more efficient at removing hydrophobic and small-sized DOM.

Geochemical weathering properties of marine terrace sediment at Gangneung-Donghae area, South Korea (강릉-동해에 분포하는 해안단구 퇴적물의 지화학적 풍화 특성)

  • Hong, Seongchan;Choi, Jeong-Heon;Kim, Jong Yeon
    • Journal of The Geomorphological Association of Korea
    • /
    • v.23 no.2
    • /
    • pp.95-108
    • /
    • 2016
  • Several flights of marine terraces were developed along the Eastern coast of Korea (Gangneung-Donghae). Various dating techniques have been applied to determine the age of these terraces, with a view to better understand the regional uplift history. In this study, we compare the major element compositions of the terrace deposits and modern beach sediments to estimate the relative formation age of these terraces. We observed a discernible difference in major element geochemistry between modern beach sediments and various elevated terrace deposit (i.e. palaeobeach sediments). In general, weathering properties of marine terrace sediments are expected to be affected by the formation ages of terraces, and here, we confirm that the chemical composition are indicative of the relative age of the terraces in this region.

Neutrophil oxidative burst as a diagnostic indicator of IgG-mediated anaphylaxis

  • Won, Dong Il;Kim, Sujeong;Lee, Eun Hee
    • BLOOD RESEARCH
    • /
    • v.53 no.4
    • /
    • pp.299-306
    • /
    • 2018
  • Background IgG-mediated anaphylaxis occurs after infusion of certain monoclonal antibody-based therapeutics. New in vitro tests are urgently needed to diagnose such reactions. We investigated whether allergens trigger neutrophil oxidative burst (OB) and if neutrophil OB occurs due to allergen-specific IgG (sIgG). Methods Neutrophil OB was measured by dihydrorhodamine 123 flow cytometry using a leukocyte suspension spiked with a very small patch of the allergen crude extract, Dermatophagoides farinae (Der f). The mean fluorescence intensity ratio of stimulated to unstimulated samples was calculated as the neutrophil oxidative index (NOI). Results The Der f-specific NOI (Der f-sNOI) showed a time-dependent increase after Der f extract addition. At 15 min activation, higher Der f-sIgG levels were associated with lower Der f-sNOI values in 31 subjects (P<0.05). This inverse relationship occurs due to the initial blocking effect of free Der f-sIgG. Additionally, neutrophil OB was nearly absent (Der f-sNOI of -1) in two cases: a subject with undetectable Der f-sIgG levels and washed leukocyte suspensions deprived of Der f-sIgG. Conclusion Allergens can trigger neutrophil OB via preexisting allergen-sIgG. Neutrophil OB can be easily measured in a leukocyte suspension spiked with the allergen. This assay can be used to diagnose IgG-mediated anaphylaxis.

Evaluation of waterlogging tolerance using chlorophyll fluorescence reaction in the seedlings of Korean ginseng (Panax ginseng C. A. Meyer) accessions (엽록소 형광반응을 이용한 인삼 유전자원의 습해 스트레스 평가)

  • Jee, Moo Geun;Hong, Young Ki;Kim, Sun Ick;Park, Yong Chan;Lee, Ka Soon;Jang, Won Suk;Kwon, A Reum;Seong, Bong Jae;Kim, Me-Sun;Cho, Yong-Gu
    • Journal of Plant Biotechnology
    • /
    • v.49 no.3
    • /
    • pp.240-249
    • /
    • 2022
  • Measuring chlorophyll fluorescence (CF) is a useful tool for assessing a plant's ability to tolerate abiotic stresses such as drought, waterlogging and high temperature. Korean ginseng is highly sensitive to water stress in paddy fields. To evaluate the possibility of non-destructively diagnosing waterlogging stress using chlorophyll fluorescence (CF) imaging techniques, we screened 57 ginseng accessions for waterlogging tolerance. To evaluate waterlogging tolerance among the 2-year-old Korean ginseng accessions, we treated ginseng plants with water stress for 25 days. The physiological disorder rate was characterized through visual assessment (an assigned score of 0-5). The physiological disorder rates of Geumjin, Geumsun and GS00-58 were lower than that of other accessions. In contrast, lines GS97-62, GS97-69 and GS98-1-5 were deemed susceptible. Root traits, chlorophyll content and the reduction rates decreased in most ginseng accessions. Further, these metrics were significantly lower in susceptible genotypes compared to resistant ones. All CF parameters showed a positive or negative response to waterlogging stress, and this response continuously increased over the treatment time among the genotypes. The CF parameter Fv/Fm was used to screen the 57 accessions, and the results showed clear differences in Fv/Fm between the susceptible and resistant genotypes. Susceptible genotypes had an especially low Fv/Fm value of less than 0.8, reflecting damage to the reaction center of photosystem II. It is concluded that Fv/Fm can be used as a CF parameter index for screening waterlogging stress tolerance in ginseng genotypes.

Characterization of PET films coated with organic-inorganic hybrid coating system containing surface modified zirconia (표면 개질된 지르코니아를 함유한 유-무기 하이브리드 코팅액으로 도포된 PET 필름의 특성)

  • Lee, Soo;Kim, Sang Yup;Kim, Young Jun
    • Journal of the Korean Applied Science and Technology
    • /
    • v.35 no.3
    • /
    • pp.595-605
    • /
    • 2018
  • In recent years, researches on organic-inorganic coating films have conducted a nanocomposite system composed of organic resin matrices having excellent flexibility and chemical stability and inorganic materials having excellent mechanical properties. The o-phenylphenoxyethyl acrylate (OPPEA) used as the acrylate monomer has a high refractive index of 1.58, and the bisphenol A ethoxylate diacrylate (BAEDA) has a low refractive index but improves the chemical stability of the organic resin. In addition, zirconia used as an inorganic material exhibits excellent durability and optical properties. In this study, the BAEDA contents in acrylate monomer were controlled to produce a film with suitable optical transparency. And optimum conditions were established by comparing the changes in surface properties of PET films detected with pencil hardness tester, Abbe's refractometer, and UV-vis spectrophotometer. The hydrophobicity and the dispersibility of zirconia in acrylate monomer were much improved after modification with ${\gamma}$-methacryloxypropyltrimethoxysilane (MPS), which is a silane coupling agent. And the existence of ester C=O bond peak at $1716cm^{-1}$ introduced by MPS through FT-IR ATR spectrophotometer confirmed the completion of surface modification of zirconia with MPS. In addition, the presence of silicon atom on the surface modified zirconia was also proved using X-ray fluorescence spectrometer. When the photocurable hybrid coating was prepared by introducing chemically modified zirconia into acrylate monomer, the refractive index of this coated PET film was improved by 1.2%, compared to the only acrylate coated PET film. The homogeneous distribution of zirconia in acrylate coating layer on PET film was also identified through SEM/EDS mapping analysis technique.

Gemological Comparison between Gwangdong Tektite and Baikdusan Obsidian (텍타이트와 흑요석의 보석학적 특징비교 - 중국 광동 텍타이트와 백두산 흑요석을 중심으로 -)

  • Kim, Won-Sa
    • Journal of the Mineralogical Society of Korea
    • /
    • v.20 no.3
    • /
    • pp.181-191
    • /
    • 2007
  • The gemological charateristics of Gwangdong tektites and Baikdusan obsidians were investigated, using microscopy, density and refractive index measurements, X-ray fluorescence spectrometry, Inductively Coupled Plasma Mass spectrometry, X-ray powder diffraction, and electron microprobe analysis. The Gwangdong tektites and Baikdusan obsidians are both black in colour and slightly trans-lucent with various shades of brown when cut into a few mm thick. Both the materials yield conchoidal fracture on broken surface. The tektites occur as tear-drop shapes, ranging from 4 to 10 cm long, and in spheres, from 3 to 5 cm in diameter. On the surface numerous shallow pits up to 3 mm in diameter are present. Mohs' hardness and specific gravity are 5 to 5.5 and 2.66, respectively. The tektites are singly refractive, with an refractive index of 1.51. Numerous spherical air bubbles are randomly scattered throughout the tektites, and silica-rich glass inclusions are occasionally seen. X-ray powder diffraction analysis verifies that they are non-crystalline. The Baikdusan obsidians show very similar properties to those of the Gwangdong tektites, especially in hardness, amorphous nature and fracture. Nevertheless, the Baikdusan obsidian can readily be distinguished from the Gwangdong tektites by refractive index ($1.49{\sim}1.50$), specific gravity (2.67 to 2.68), and inclusions (absence of bubbles and presence of sanidine and magnetite crystals).

Evaluation of Photochemical Reflectance Index (PRI) Response to Soybean Drought stress under Climate Change Conditions (기후변화 조건에서 콩 한발스트레스에 대한 광화학 반사 지수 반응 평가)

  • Sang, Wan-Gyu;Kim, Jun-Hwan;Shin, Pyeong;Baek, Jae-Kyeong;Lee, Yun-Ho;Cho, Jung-Il;Seo, Myung-Chul
    • Korean Journal of Agricultural and Forest Meteorology
    • /
    • v.21 no.4
    • /
    • pp.261-268
    • /
    • 2019
  • Climate change and drought stress are having profound impacts on crop growth and development by altering crop physiological processes including photosynthetic activity. But finding a rapid, efficient, and non-destructive method for estimating environmental stress responses in the leaf and canopy is still a difficult issue for remote sensing research. We compared the relationships between photochemical reflectance index(PRI) and various optical and experimental indices on soybean drought stress under climate change conditions. Canopy photosynthesis trait, biomass change, chlorophyll fluorescence(Fv/Fm), stomatal conductance showed significant correlations with midday PRI value across the drought stress period under various climate conditions. In high temperature treatment, PRI were more sensitive to enhanced drought stress, demonstrating the negative effect of the high temperature on the drought stress. But high CO2 concentration alleviated the midday depression of both photosynthesis and PRI. Although air temperature and CO2 concentration could affect PRI interpretation and assessment of canopy radiation use efficiency(RUE), PRI was significantly correlated with canopy RUE both under climate change and drought stress conditions, indicating the applicability of PRI for tracking the drought stress responses in soybean. However, it is necessary to develop an integrated model for stress diagnosis using PRI at canopy level by minimizing the influence of physical and physiological factors on PRI and incorporating the effects of other vegetation indices.

Study on the Evaluation of Thermal Damage According to the Manufacturing Conditions of Korean Paper (한지의 제조 조건에 따른 열 손상 평가 연구)

  • Kim, Ji Won;Park, Se Rin;Han, Ki Ok;Jeong, Seon Hwa
    • Journal of Conservation Science
    • /
    • v.37 no.6
    • /
    • pp.648-658
    • /
    • 2021
  • In this study, we aimed to analyze the chemical changes that occur in Korean paper in an accelerated deterioration environment of 105℃. We selected the Korean paper produced with different types of cooking agents (plant lye, Na2CO3) and during different manufacturing seasons (winter, summer). The degree of deterioration of the Korean paper was confirmed by measuring the brightness, yellowness, and pH level, and the degree of change in each vibrational region of cellulose as deterioration progressed through infrared (FT-IR) spectroscopy. The FT-IR analysis showed that, as deterioration progressed, the absorbance of the amorphous region in cellulose decreased, whereas the absorbance of the crystalline region slightly increased. X-Ray diffraction (XRD) analysis and Raman spectroscopy were performed to verify the changes in the crystalline and amorphous regions in cellulose indicated by the FT-IR results. Furthermore, the crystallinity index (CI) was calculated; it showed a slight increase after deterioration; therefore, CI was confirmed to follow the same trend as that observed for absorbance in the FT-IR results. In addition, as a result of Raman spectroscopic analysis, the degree of decomposition of the amorphous region in the cellulose under the manufacturing conditions was confirmed by the fluorescence measured after the deterioration.

Effects of strong shading on growth and yield in sweet potato (Ipomoea batatas L. LAMK.)

  • Shin, Jong Moo;Song, Seon Hwa;Park, Chan Young;Lee, Hyeon Young;Shim, Sang In
    • Proceedings of the Korean Society of Crop Science Conference
    • /
    • 2017.06a
    • /
    • pp.241-241
    • /
    • 2017
  • Sweet potatoes (Ipomoea batatas (L.) LAMK.,) have been cultivated in Central and South America for about 2000 years and are now grown mainly in Asia and South America. Sweet potatoes are annual in the temperate region, but are classified as perennial in the tropical region. In 2000, the cultivation area of sweet potatoes decreased to about 16,000 ha in 2000, but the cultivation area increased slightly in recent 20,000 ha in Korea. Sweet potatoes do not show higher maximum dry matter production of 120 ~ 150g per plant, and the leaf area index (LAI), which maximizes dry matter production, is known as 3.0 ~ 4.0. As the leaf area increase, the penetration of light into the canopy becomes poor, and sufficient photosynthesis cannot be achieved in the lower leaves, on the other hand the respiration increase, which results in poor dry matter production. This study was conducted to know the responses of sweet potatoes to intensive shading treatment of 80% shading. This experiment was conducted for about 42 days from September 6, 2016 to October 18, 2016 at Gyeongsang National University Experimental Farm, Jinju, Korea. The plant canopy was shaded with black nylon 80% shade cloth suspended 1.2 m above the ground. The photosynthetic rate, stomatal conductance, chlorophyll fluorescence, SPAD and NDVI were measured in 3 replicates every 7 days after shading initiation. After the fresh weight was measured, the samples were dried at $80^{\circ}C$ in a dry oven and measured. By the 80% shading treatment, chlorophyll fluorescence of the treated plants was slightly higher than that of the control, the SPAD value was higher by 3.4 and NDVI value was higher by 0.01. However, photosynthetic rate and stomatal conductance were lower than those of the control. The stomatal conductance of the control were two times higher than those of the control and the photosynthetic rate of the control was four times higher than that of the control. In control, plant showed a tendency to steadily increase in fresh weight and dry weight. However, in the case of shading treatment, the tendency to increase in the fresh and dry weight of tuberous roots was not clear. The fresh weight of shoot showed a tendency to increase steadily while the difference between treatment and control was not large, but tended to decrease after frost.

  • PDF

Amount of Telomeric DNA on Pig Lymphocytes by Quantitative Fluorescence in situ Hybridization (양적형광접합보인법(Q-FISH)에 의한 돼지 백혈구 세포의 텔로미어 함량 분석)

  • Sohn, Sea-Hwan;Jung, Hyun-Jin;Choi, Duk-Soon
    • Journal of Animal Science and Technology
    • /
    • v.50 no.4
    • /
    • pp.465-474
    • /
    • 2008
  • The amount of telomeric DNA was quantified across different breeds(Landrace, Duroc, Yorkshire and Berksire), at different ages(90 days old and 180 days old) and among sexes(male and female) in pigs raised at the Performance Testing Station of Korea Swine Association, Jinkyo, Korea. The telomeric DNA amount was quantified by Quantitative Fluorescence In Situ Hybridization(Q-FISH) using a porcine telomeric DNA probe on interphase nuclei of lymphocytes. Analysis revealed that the amount of telomeric DNA on the pig lymphocytes was found to decrease with age. The quantity of telomeres significantly differed among breeds at 90 days of age. The colored breeds such as Berkshire and Duroc had higher amount of telomeric DNA than the Yorkshire and Landrace breed. In addition, the amount of telomeric DNA in male lymphocytes was significantly higher than that of females. In the correlation coefficients between the telomere quantity and their productive traits; average daily gain, loin percent and index value were positively correlated, whereas body length, feed efficiency and back fat thickness correlated negatively. However, the correlation coefficients were very low and not significant. Therefore, this study suggests that the amount of telomeres on lymphocytes can be considered as a physiological marker but not as a productive marker in pig.