• 원예과학기술지
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• 제31권4호
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• pp.400-406
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• 2013

This study was conducted to investigate the growth and flowering of African marigold (Tagetes erecta L.) and salvia (Salvia splendens F. Sello ex Ruem & Schult.) seedlings before and after storage under fluorescent lamps and green LED radiation conditions with different light intensities during storage. The both seedlings were kept under a storage room controlled at $8^{\circ}C$ air temperature and $40{\pm}10%$ relative humidity conditions. Light intensities were maintained at 15 and $30{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ during storage. In lighting treatments, dry weight of African marigold at 28 days after storage was not significantly different, and decreased approximately 29% compared to pre-storage under dark treatment. There was no significant difference in the leaf area of salvia seedlings stored under dark condition compared to before storage, but the leaf area under green light radiation with higher light intensity (treatment GH) was two times greater than before storage. The survival rate after transplanting of African marigold stored under dark condition was 10%, and days to flowering increased compared to those stored under fluorescent and green light with higher light intensity (treatment FLH, GH). Comparing to before storage, growth and flowering of the both seedlings after storage were significantly promoted by the light exposure during storage. The present experimental results show that the light intensity should be decided to maintain minimum growth during lighting storage and storage quality of the seedlings such as flowering promotion and extended blooming period after lighting treatment during storage period from the above results.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.27-39
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• 2005

a-Ketol linolenic acid [KODA, 9,10-ketol-octadecadienoic acid, or 9-hydroxy-10 -oxo-12(Z), 15(Z)-octadecadienoic acid] was found as a stress-induced factor in Lemna paucicostata. KODA reacts with catecholamines to generate many products that strongly induce flowering in L. paucicostata, although KODA itself was inactive. KODA contains an asymmetric carbon at the 9-position in the molecule; the 9-hydroxyl group is predominantly 9R, with an enantiomeric excess of 40% (70% 9R and 30% 9S). We analyzed two major products of the reaction between KODA and norepinephrine, named FN1 and FN2. FN1 was identified as a tricyclic a-ketol fatty acid, 9(R)-11-{(2'R,8’R,10'S,11'S)-2',8'-dihydroxy-7'-oxo-11'-[(Z)-2-pentenyl]-9'-oxa-4'-azatricyclo[6.3.1.01.5]dodec-5'en-10'-yl}-9-hydroxy-10-oxoundecanoic acid. FN2 was the C-9 epimer of FN1. FN1 was derived from 9R-type KODA and FN2 from 9S-type. FN1 showed strong flower-inducing activity, but FN2 was inactive. Pharbitis nil (violet) is a typical short-day plant; flowering can be induced by exposing a seedling cultivated under continuous light to a single 16-h dark period. We analyzed endogenous KODA levels and showed that they were closely related to flower induction: KODA sharply increased in the later part of a 16-h dark period, on the other hand, it failed to increase in the night-break experiment. In addition to it, KODA increased transiently in immature flower buds in all the plants we examined, including P. nil. No such increase of KODA was seen in foliar buds of P. nil. When KODA was sprayed on seedlings of Pharbitis, flower induction was promoted only by the (R)-form of KODA. We also found that KODA enhances flowering in garden plants such as carnations and impatienses. These phenomena indicate that KODA may be involved in flowering formationg of plants and it is potentially useful for a regulating agent for commercial plant flowering.

• 한국약용작물학회지
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• 제14권4호
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• pp.239-243
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• 2006

개화 이후 고사하는 일임성 식물인 바위솔은 가을철에 집중되는 자연산의 출하시기와 중복되지 않도록 비가림 하우스에서 인공재배되고 있다. 본 연구는 하우스 시설로 인한 차광 (무차광, 35, 55, 75, 95% 차광)이 바위솔의 생장 및 개화에 미치는 영향을 파악하고자 실시되었던 바 그 결과를 요약하면 다음과 같다. 1. 초장, 화서장, 엽과 포엽수 및 경직경은 광도가 가장 약한 95% 차광시 가장 짧고, 적거나, 가늘었던 반면, 여타 차광 처리간에는 차이가 적다 할지라도 차광이 심할수록 줄어드는 경향을 보였다. 2. 엽과 포엽중, 경중, 근중, 지상부중, 전체건물중은 무차광에서 가장 많았으며 차광이 심할수록 감소되는 경향을 보였다. 3. 형성된 소화수는 건물중과 같이 무차광에서 가장 많고 차광이 심할수록 감소되었으나, 95% 차광시 아주 적었다. 4. 차광처리로 소화가 개화된 개체는 관찰되지 않아 광도가 제한되는 시설하우스에서 여타 처리를 통하여 바위솔의 인공재배는 가능할 것으로 판단된다.

• 한국작물학회지
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• 제41권1호
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• pp.103-108
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• 1996

풋콩의 품종별, 수확시기에 따른 품질특성의 변화를 관찰하여 얻어진 결과를 요약하면 다음과 같다. 1. 공시품종의 100립중은 4가지 모두 개화후 43일까지는 증가하였으며, 그 이후에는 감소하는 경향을 나타내었다. 2. 당함량은 도우야와 화엄풋콩의 경우 총당과 sucrose함량이 초기에 높다가 개화후 37일 이후에 감소하는 경향을 나타내었으며, 시로후미는 개화후 40일에 총당함량이 급격히 감소하였다가 개화후 43일부터 증가하는 경향을 보여주었으며, 시로후사는 당함량이 일정한 수준을 유지하였다. 3. 비타민 C 함량은 도우야와 시로후미는 개화후 37일 이후에 급격히 증가하다자 평형상태를 보였고, 시로후사와 화엄풋콩은 개화후 43일경에 최고치를 나타내었다. 4. 풋콩의 texture측정법으로 주로 사용하는 경도의 변화를 살펴본 결과, 개화후 일수가 증가할수록 경도가 증가하는 경향을 볼 수 있었다. 5. 수확시기별, 품종별 관능특성을 알아 본 결과, 수확시기에 따른 기호도의 차이가 나타났으며, 또한 관능특성간의 상관관계에서는 고소한 맛과 비린 맛이 강한 부의 상관을 나타내었다.

• 한국약용작물학회지
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• 제26권3호
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• pp.214-219
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• 2018

Background: This study was carried out to determine the best time for collecting ginseng berries without reducing the ginsenoside-Re content of ginseng roots, which are used as food, medicine, or cosmetic materials. Methods and Results: The test variety of ginseng used in this study was is Chunpung, which was collected from a 4-year-old ginseng field. Ginseng berries were collected at 7, 14, 21, 28, 35, 42, 49, and 56 days after flowering. The number of berry bunches per $1.62m^2$ ranged from 43.4 to 61.4, while the weight of berries per $1.62m^2$ was the greatest when they were collected 49 days after flowering. The root fresh weight per $1.62m^2$ was increased by 0.21 - 1.00 kg compared with that before the test, but root weight gain was decreased as the berry collection time was delayed. Total ginsenoside content of 4-year-old ginseng was the highest when berries were collected 7 days after flowering, while the ginsenoside-Re contents was the highest when collection was done 14 days after flowering. Conclusions: The most suitable period for ginseng berry collection was proposed to be from 14 to 21 days after flowering, as this is when the content of ginsenoside-Re, which is useful as a medicinal or cosmetic material, is still high and the ginseng root has not yet decreased in weight.

• 원예과학기술지
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• 제17권6호
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• pp.755-757
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• 1999

본 연구는 동양란 '철골소심'의 개화증진을 위하여 BA처리후 적정한 관리온도를 알아보기 위해 수행되었다. 개화율은 주야간의 기온이 $30/25^{\circ}C$을 유지했을 때 90%, $25/20^{\circ}C$와 대조구에서 50%로 나타났으며 $20/15^{\circ}C$처리에서는 전혀 개화가 되지 않았다. 기형화율은 $25/20^{\circ}C$에서는 나타나지 않았고 $30/25^{\circ}C$에서는 4%, 대조구에서는 9%로 나타났다. $30/25^{\circ}C$에서 화경장, 화경수 등이 증가하였고 화아품질이 향상되었다. BA 살포 후 철골소심의 개화 유도를 위한 한계 기온은 $20^{\circ}C$ 이상인 것으로 나타났다.

• 식물조직배양학회지
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• 제26권2호
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• pp.109-113
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• 1999

인삼의 기내 화아형성과 cytokinin과의 관계를 조사하기 위하여 접합자배, 유식물체, 자엽마디 절편체를 cytokinin(BA, kinetin, 2-iP, zeatin) 5 $\mu$M 단독 혹은 GA$_3$ 5 $\mu$M와 함께 MS 배지에서 배양하였다. 화아형성은 재료에 관계없이 BA처리구에서 가장 높게 나타났으며, kinetin, 2-iP, zeatin 순으로 나타났다. Cytokinin과 GA$_3$를 함께 첨가한 경우에는 특히 자엽마디 절편체 재료에서 화아형성 빈도가 현저히 증가하여 BA와 함께 처리하였을 때는 100% 화아형성을 나타내었다. 사용한 cytokinin은 adenine 기본골격과 다양한 측쇄구조 (-R)로 이루어져 있는데 이 측쇄구조의 분배계수 (logP)가 인삼의 기내 화아형성과 높은 상관성을 나타내어 cytokinin류의 지용성이 화아형성에 중요하게 관여함이 시사되었다.

• Journal of Ecology and Environment
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• 제41권9호
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• pp.256-264
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• 2017

Background: The aim of this study was to clarify the relationship between the sexual reproduction and the resource allocation in a natural Polygonatum humile population grown in a temperate mixed forest gap. For this aim, the plant size, the node which flower was formed, the fruiting rate, and the dry weight of each organ were monitored from June 2014 to August 2015. Results: Firstly, in 3-13-leaf plants, plants with leaves ${\leq}8$ did not have flowers and in plants with over 9 leaves the flowering rate increased with the number of leaves. Among plants with the same number of leaves, the total leaf area and dry weight of flowering plants were larger than those of non-flowering plants. The minimum leaf area and dry weight of flowering plants were $100cm^2$ and 200 mg, respectively. Secondary, the flowers were formed at the 3rd~8th nodes, and the flowering rate was highest at the 5th node. Thirdly, cumulative values of leaf properties from the last leaf (the top leaf on a stem) to the same leaf rank were greater in a plant with a reproductive organ than in a plant without a reproductive organ. Fourthly, fruit set was 6.1% and faithful fruit was 2.6% of total flowers. Biomasses of new rhizomes produced per milligram dry weight of leaf were $0.397{\pm}190mg$ in plants that set fruit and $0.520{\pm}0.263mg$ in plants that did not, and the difference between the 2 plant groups was significant at the 0.1% level. Conclusions: P. humile showed that the 1st flower formed on the 3rd node from the shoot's base. And P. humile showed the minimum plant size needed in fruiting, and fruiting restricted the growth of new rhizomes. However, the fruiting rate was very low. Thus, it was thought that the low fruiting rate caused more energy to invest in the rhizomes, leading to a longer rhizome. A longer rhizome was thought to be more advantageous than a short one to avoid the shading.

• BMB Reports
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• 제51권4호
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• pp.163-164
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• 2018

CONSTANS (CO) induces the expression of FLOWERING LOCUS T (FT) in the photoperiodic pathway, and thereby regulates the seasonal timing of flowering. CO expression is induced and CO protein is stabilized by FLAVIN-BINDING KELCH REPEAT F-BOX PROTEIN 1 (FKF1) in the late afternoon, while CO is degraded by CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) during the night. These regulatory cascades were thought to act independently. In our study, we investigated the relationship between FKF1 and COP1 in the regulation of CO stability in response to ambient light conditions. A genetic analysis revealed that FKF1 acts as a direct upstream negative regulator of COP1, in which cop1 mutation is epistatic to fkf1 mutation in the photoperiodic regulation of flowering. COP1 activity requires the formation of a hetero-tetramer with SUPPRESSOR OF PHYA-105 (SPA1), [$(COP1)_2(SPA1)_2$]. Light-activated FKF1 has an increased binding capacity for COP1, forming a FKF1-COP1 hetero-dimer, and inhibiting COP1 homo-dimerization at its coiled-coil (CC) domain. Mutations in the CC domain result in poor COP1 dimerization and misregulation of photoperiodic floral induction. We propose that FKF1 represses COP1 activity by inhibiting COP1 dimerization in the late afternoon under long-day conditions, resulting in early flowering.

• Molecules and Cells
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• 제41권8호
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• pp.781-798
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• 2018

Plants have evolved strategies to cope with drought stress by maximizing physiological capacity and adjusting developmental processes such as flowering time. The WOX13 orthologous group is the most conserved among the clade of WOX homeodomain-containing proteins and is found to function in both drought stress and flower development. In this study, we isolated and characterized OsWOX13 from rice. OsWOX13 was regulated spatially in vegetative organs but temporally in flowers and seeds. Overexpression of OsWOX13 (OsWOX13-ov) in rice under the rab21 promoter resulted in drought resistance and early flowering by 7-10 days. Screening of gene expression profiles in mature leaf and panicles of OsWOX13-ov showed a broad spectrum of effects on biological processes, such as abiotic and biotic stresses, exerting a cross-talk between responses. Protein binding microarray and electrophoretic mobility shift assay analyses supported ATTGATTG as the putative cis-element binding of OsWOX13. OsDREB1A and OsDREB1F, drought stress response transcription factors, contain ATTGATTG motif(s) in their promoters and are preferentially expressed in OsWOX13-ov. In addition, Heading date 3a and OsMADS14, regulators in the flowering pathway and development, were enhanced in OsWOX13-ov. These results suggest that OsWOX13 mediates the stress response and early flowering and, thus, may be a regulator of genes involved in drought escape.