• Asian Pacific Journal of Cancer Prevention
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• 제15권17호
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• pp.7297-7301
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• 2014

Objective: To explore the radiosensitization effect of overexpression of silent information regulator 6 (SIRT6) on A549 non-small cell lung cancer (NSCLC) cells. Methods: Adenovirus vector Ad-SIRT6 causing overexpression of SIRT6 was established. Western blotting and MTT assay were adopted to detect the level of SIRT6 protein and the inhibitory rate of A549 cell proliferation after different concentrations of adenovirus transduction (0, 25, 100, 200, and 400 pfu/cell) for 24 h. Control group, Ad-null group and Ad-SIRT6 group were designed in this experiment and virus concentration of the latter two groups was 200 pfu/cell. Colony formation assays were employed to test survival fraction (SF) of the 3 groups after 0, 2, 4, 6, 8, 10 X-ray irradiation. Flow cytometry was used to detect the status of cell cycle of 3 groups after 48 h of 4Gy X-ray irradiation and Western blotting was used to determine the expression of apoptosis-related genes of 3 groups after 48 h of 4GyX-ray irradiation. Results: In the range of 25~400 pfu/cell, the inhibitory rate of A549 cell proliferation increased as adenovirus concentration raised. The inhibitory rates under the concentrations of 0, 25, 100, 200, and 400 pfu/cell were 0%, $4.23{\pm}0.34%$, $12.7{\pm}2.57%$, $22.6{\pm}3.38%$, $32.2{\pm}3.22%$, $38.7{\pm}4.09%$ and $47.8{\pm}5.58%$ and there were significantly differences among groups (P<0.05). SF in Ad-SIRT6 group was lower than Ad-null and control groups after 4~10Gy X-ray irradiation (P<0.05) and the sensitization enhancement ratio (SER) was 1.35 when compared with control group. Moreover, after 48 h of 4Gy X-ray irradiation, there appeared a significant increase in G1-phase cell proportion, upregulated expression of the level of apoptosis-promoting genes (Bax and Cleaved caspase-3), but a obvious decline in S-phase and G2-phase cell proportion and a significant decrease of the level of apoptosis-inhibiting gene (Bal-2) in the Ad-SIRT6 group (P<0.05). Conclusion: The over-expression of adenovirus-mediated SIRT6, which has radiosensitization effect on A549 cells of NSCLC, can inhibit the proliferation of A549 cells and cause G0/G1 phase retardation as well as induce apoptosis of cells.

• 한국항공우주학회지
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• 제41권4호
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• pp.291-298
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• 2013

본 논문에서는 압력보상형 사판식 유압 피스톤 펌프와 밸브 제어형 실린더를 결합한 EHA에 대하여 유압 실린더의 소비 유량이 작으면 펌프 회전 속도를 낮추는 새로운 개념의 하이브리드형 제어 시스템을 제안하였다. 펌프 내 압력조절기의 사판각 제어와 간섭을 피하기 위해 위치 명령 신호의 속도 성분 평균치를 이용하여 펌프의 회전속도를 조절하였고, 시스템 압력이 기준치 이하로 낮아지는 것을 방지하기 위해 압력 스위치 기능을 추가하였다. 시뮬레이션과 실험 결과에 의하면, EHA의 동적인 응답 특성에 영향을 주지 않는 조건에서 하이브리드 제어를 통해 공전 모드에서의 펌프 회전속도를 1,800rpm에서 600rpm로 낮춤으로써 하이브리드 제어를 안 할 경우에 비해 펌프 구동 동력을 약 44%까지 절감시킬 수 있음을 확인하였다.

• 동의생리병리학회지
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• 제18권5호
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• pp.1397-1403
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• 2004

By recently study, GM (Gamipaemo-tang) treatment have worked well on the allergic asthma. The purpose of this study was effect of GM extract on helper T cell, major regulator of immune system. Splenic cells from 8-week BALB/c mice were cultured in GM containing media without activation for 48 hours. The MTS assay and flow cytometry study revealed that lymphocyte treated with GM were not effective on CD4+ T cells. Subsequently CD4+ T cells were isolated and cultured in GM containing media. Either GM were not effective on CD4+ T cell without APCs. By FACS scan analysis, the expression of INF-γ, IL-4 were down-regulated in the condition skewed Th1 and Th2 cells respectively, Using ELISA analysis, the expression of INF-γ is up-regulated and IL-4 is down-regulated in the condition skewed Th1, Th2 cells respectively. With RT-PCR analysis, the expression of mRNA for INF-γ is down-regulated and IL-4 is down-regulated in the condition skewed Th1 and Th2 cells respectively. The result suggests that GM inhibited the differetiation of Th2 cells significantly and indicates GM could enhance anti-allergic immune system.

• Journal of Ginseng Research
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• 제33권1호
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• pp.65-71
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• 2009

This experiment was done to determine the optimum conditions for the induction of tetraploidy in Panax ginseng C. A. Meyer using bud length, temperature and plant growth regulator pretreatments. Highest callus formation was obtained when the medium was inoculated with flower bud in the size of 2-3 mm in length. The optimum temperature for the callus formation was high when treated at $4^{\circ}C$ for 4-5 days. Among the treatments of growth regulators and different concentration, highest callus formation was observed in combination of 5 mg/L 2,4-D and 1 mg/L kinetin for P. ginseng. As a result of flow cytometer analysis, all 7 adventitious roots were confirmed as tetraploidys. Cytological analysis revealed that the chromosome number of tetraploid roots was 96, while that of diploid roots was 48. Tetraploid ginseng roots were inoculated to flower bud size of 2-3 mm in length. The callus formation was optimum when treated with 1 mg/L 2,4-D at $4^{\circ}C$ for 5 days. Compared with control roots, tetraploid roots were thicker and longer and had few lateral branches. Fresh weight of tetraploid roots was relatively higher than the control roots.

• 한국가스학회지
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• 제23권6호
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• pp.25-32
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• 2019

많은 대량소비 가스시설에서는 천연가스를 가스공사의 배관망을 통해 공급받지 못하여 탱크로리를 통해 액화천연가스(LNG)를 가스공사로부터 공급받아 기화시켜 사용한다. 이러한 시설에서 가스 누출로 인한 인명 또는 재산피해를 방지하기 위하여 주입하는 부취제가, 최초지점과 소비지점들에서 어떤 농도로 분포하는지 분석하는 연구를 수행하였다. 일정한 유량이 흐를 때 가스시설내의 배관위치에 따라 부취농도의 변화를 확인하기 위한 분석이 이루어졌다. 시료채취는 알루미늄 재질의 4.5 L 용기를 진공상태로 만든 후, 부취제가 주입되는 정압기와 가스가 사용되는 소비지점에서 시료를 포집하였다. 포집된 가스시료로부터 가스크로마토그래피(Main Body : Agilent 7890A, Detector : ANTEC 7090)를 이용하여 부취제의 농도를 분석하였다. 안전한 가스시설관리를 위하여 대량수요자 시설의 형태별로 부취농도 관리방법의 정립이 필요한 것으로 판단된다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권8호
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• pp.4853-4858
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• 2013

Objectives: To investigate whether hypoxia has an effect on regulation of multidrug resistance (MDR) to chemotherapeutic drugs in laryngeal carcinoma cells and explore the role of hypoxia-inducible factor-$1{\alpha}$ (HIF-$1{\alpha}$). Methods: Laryngeal cancer cells were cultured under normoxic and hypoxic conditions. The sensitivity of the cells to multiple drugs and levels of apoptosis induced by paclitaxel were determined by MTT assay and annexin-V/propidium iodide staining analysis, respectively. HIF-$1{\alpha}$ expression was blocked by RNA interference. The expression of HIF-$1{\alpha}$ gene was detected by real-time quantitative RT-PCR and Western blotting. The value of fluorescence intensity of intracellular adriamycin accumulation and retention in cells was evaluated by flow cytometry. Results: The sensitivity to multiple chemotherapy agents and induction of apoptosis by paclitaxel could be reduced by hypoxia (P<0.05). A the same time, the adriamycin releasing index of cells was increased (P<0.05). However, resistance acquisition subject to hypoxia in vitro was suppressed by down-regulating HIF-$1{\alpha}$ expression. Conclusion: HIF-$1{\alpha}$ could be considered as a key regulator for mediating hypoxia-induced MDR in laryngeal cancer cells via inhibition of drug-induced apoptosis and decrease in intracellular drug accumulation.

• Asian Pacific Journal of Cancer Prevention
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• 제14권2호
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• pp.747-752
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• 2013

Oncostatin M (OSM) is a multifunctional cellular regulator acting on a wide variety of cells, which has potential roles in the regulation of gene activation, cell survival, proliferation and differentiation. Previous studies have shown that OSM can induce morphological and/or functional differentiation and maturation of many tumor cells. However, the action of OSM on the induction of differentiation of human hepatocellular carcinoma (HCC) has not been reported. Here, we investigated the effects of different concentrations of OSM on human HCC cell line SMMC-7721 growth, proliferation, cell cycling, apoptosis and differentiation in vitro. Cell growth was determined via MTT assay, proliferation by cell cycle analysis, apoptosis by flow cytometry, morphology by transmission electronic microscopy, and cell function by detection of biochemical markers. Our results demonstrated that OSM strongly inhibited the growth of SMMC-7721 cells in a dose-dependent manner, associated with decreased clonogenicity. Cell cycle analysis revealed a decreased proportion of cells in S phase, with arrest at G0/G1. The apotosis rate was increased after OSM treatment compared to the control. These changes were associated with striking changes in cellular morphology, toward a more mature hepatic phenotype, accompanied by significant reduction of the expression of AFP and specific activity of ${\gamma}$-GT, with remarkable increase in secretion of albumin and ALP activity. Taken together, our findings indicate that OSM could induce the differentiation and reduce cell viability of SMMC-7721 cells, suggesting that differentiation therapy with OSM offers the opportunity for therapeutic intervention in HCC.

• 전기학회논문지
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• 제67권8호
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• pp.1031-1039
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• 2018

If large-scale PV systems are continuously interconnected to distribution system, customer voltages could violate the allowable voltage limit($220{\pm}13V$) according to reverse power flow of PV system. In order to solve these problems, this paper proposes flexible adoption evaluation algorithm of PV system in distribution system which estimates proper introduction capacity and location of ESS(energy storage system) for keeping customer voltages within allowable voltage limit based on various operating scenarios related with connecting point and capacity of PV system. And also this paper proposes modeling of ESS, SVR(step voltage regulator) and PV system using PSCAD/EMTDC S/W and analyzes characteristics of customer voltages and the adoption ability of PV system in distribution system. From the simulation results, it is confirmed that proposed algorithm is useful for large-scale adoption of PV system in distribution system to maintain customer voltages within allowable voltage limit.

• 생물환경조절학회지
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• 제18권2호
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• pp.137-141
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• 2009

분주 크기와 배지 종류가 고추냉이 생존율과 생육에 미치는 영향을 알아보고자 환경이 조절된 생장상에서 60일간 재배하였다. 수태로 감싼 근경 크기(직경) 5mm 이상 분주 묘의 생존율은 100%였으며, 생육(초장 12cm 내외, 엽수 $3{\sim}4$장)은 양호하였다. 생육 60일의 분주 묘는 초장 20cm 이상, 엽수가 $1{\sim}2$장 증가하였다. 루톤 분의제 처리는 생존율과 생육에 효과가 없었다. 배지 종류(마사, 난석)에 따른 직경 $5{\sim}10$mm 크기의 분주 묘를 담액 수경으로 재배하였을 때 83% 이상의 생존율을 보였으며 배지 종류에 따른 생육 차이는 없었다. 따라서 근경 크기 5mm 이상의 고추냉이 분주를 환경이 조절된 생장상에서 30일간 수경재배 방식으로 순화하여 고추냉이 건전 묘를 생산할 수 있다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제18권1호
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• pp.153-163
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• 1996

Macrophage inflammatory protein $(MIP)-1{\alpha}$ is a cytokine which produces wide range of bioactivities such as proinflammatory, immunomodulatory, and hematopoietic modulatory actions. To determine whether $MIP-1{\alpha}$ acts as a negative regulator on the functions of lymphocyte, $[^3H]$-thymidine incorporation test and flow cytometric analysis were performed by using human tonsil T cell, human peripheral blood T cell, and murine cytolytic T lymphocyte (CTL) line CTLL-2, The results were as follow. 1. When human tonsil T lymphocytes were stimulated with anti-CD3 monoclonal antibody (mAb), rate of T cell proliferation was about four times increased. 200ng/ml of $MIP-1{\alpha}$ inhibited anti-CD3 mAb-mediated T cell growth as much as 60% (P<0.05). 2. The suppression of human peripheral T cell proliferation produced by $MIP-1{\alpha}$ was dramatic, but variable among T cells derived from different individuals $(40%{\sim}90%)$. 3. $MIP-1{\alpha}$inhibited the proliferation of murine CTL line CTLL-2 as much as 75%(P<0.001). 4. When the $MIP-1{\alpha}$ was added to human peripheral T cell, cell proporation of $CD4^+$ helper T cell and $CD8^+$ CTL were not noticeably affected. The expression level of CD4, not of Cd8, however, was down regulated by $MIP-1{\alpha}$ treatment $(27%{\sim}82%)$.