• KSII Transactions on Internet and Information Systems (TIIS)
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• v.15 no.10
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• pp.3858-3874
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• 2021

As an essential part of the urban transportation system, precise perception of the traffic flow parameters at the traffic signal intersection ensures traffic safety and fully improves the intersection's capacity. Traditional detection methods of road traffic flow parameter can be divided into the micro and the macro. The microscopic detection methods include geomagnetic induction coil technology, aerial detection technology based on the unmanned aerial vehicles (UAV) and camera video detection technology based on the fixed scene. The macroscopic detection methods include floating car data analysis technology. All the above methods have their advantages and disadvantages. Recently, indoor location methods based on wireless signals have attracted wide attention due to their applicability and low cost. This paper extends the wireless signal indoor location method to the outdoor intersection scene for traffic flow parameter estimation. In this paper, the detection scene is constructed at the intersection based on the received signal strength indication (RSSI) ranging technology extracted from the wireless signal. We extracted the RSSI data from the wireless signals sent to the road side unit (RSU) by the vehicle nodes, calibrated the RSSI ranging model, and finally obtained the traffic flow parameters of the intersection entrance road. We measured the average speed of traffic flow through multiple simulation experiments, the trajectory of traffic flow, and the spatiotemporal map at a single intersection inlet. Finally, we obtained the queue length of the inlet lane at the intersection. The simulation results of the experiment show that the RSSI ranging positioning method based on wireless signals can accurately estimate the traffic flow parameters at the intersection, which also provides a foundation for accurately estimating the traffic flow state in the future era of the Internet of Vehicles.

• Journal of Life Science
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• v.23 no.6
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• pp.832-837
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• 2013

In this study, it was investigated the possible mechanisms by which glutamine deprivation exerts its anti-proliferative action in cultured human prostate carcinoma PC3 cells. Glutamine deprivation resulted in inhibition of growth and G2/M arrest of the cell cycle in a time-dependent manner without apoptosis induction, as determined by MTT assay, DAPI staining and flow cytometry analyses. The induction of G2/M arrest by glutamine deprivation was associated with the inhibition of expression of Cdc2, cyclin A and cyclin B1, and up-regulation of the expression of cyclin-dependent kinase (Cdk) inhibitor p21(WAF1/CIP1) in both transcriptional and translational levels. Moreover, glutamine deprivation increased the phosphorylation of checkpoint kinase (Chk)1 and Chk2; however, the levels of Cdc25C phosphorylation were decreased in response to glutamine deprivation in a time-dependent manner. Our data provide a first biochemical evidence that glutamine deprivation suppresses cell viability through G2/M phase arrest without induction of apoptosis in PC3 cells.

• Biomolecules & Therapeutics
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• v.17 no.3
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• pp.282-287
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• 2009

Ochnaflavone is a natural biflavonoid and mainly found in the caulis of Lonicera japonica (Caprifoliaceae). Biological activities such as anti-inflammatory and anti-atherogenic effects have been previously reported. The anticancer activity of ochnaflavone, however, has been poorly elucidated yet. In the present study, we investigated the effect of ochnaflavone on the growth inhibitory activity in cultured human colon cancer cell line HCT-15. Ochnaflavone inhibited the proliferation of the cancer cells with an $IC_{50}$ value of $4.1{\mu}M$. Flow cytometric analysis showed that ochnaflavone arrested cell cycle progression in the G2/M phase, and induced the increase of sub-G1 peak in a concentration-dependent manner. Induction of cell cycle arrest was correlated with the modulation of the expression of cell cycle regulating proteins including cdc2 (Tyr15), cyclin A, cyclin B1 and cyclin E. The increase of sub-G1 peak by the higher concentrations of ochnaflavone (over $20{\mu}M$) was closely related to the induction of apoptosis, which was evidenced by the induction of DNA fragmentation, activation of caspase-3, -8 and -9, and cleavage of poly-(ADP-ribose) polymerase. These findings suggest that the cell cycle arrest and induction of apoptosis might be one possible mechanism of actions for the anti-proliferative activity of ochnaflavone in human colon cancer cells.

• Journal of Microbiology and Biotechnology
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• v.20 no.2
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• pp.433-437
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• 2010

In the course of screening for novel modulators of cell cycle progression and apoptosis as anticancer drug candidates, we generated an analog of sangivamycin, MCS-C2, which was elucidated as 4-amino-6-bromo-7-cyclopentyl-7H-pyrrolo[2,3-d]pyrimidine-5-carboxamide. In the present study, we evaluated the molecular mechanisms of MCSC2-induced cell cycle arrest and apoptosis in A549 human lung cancer cells. To investigate the effects of MCS-C2 on cell cycle progression in A549 cells, we measured the DNA content of A549 cells treated with $5\;{\mu}M$ MCS-C2 using flow cytometry. The analysis revealed an appreciable $G_2$ phase arrest in treated cells. This event was associated with significant upregulation of p53 and $p21^{Cip1}$. In addition, the TUNEL assay was used to examine apoptotic induction in treated cells, and the effects of MCS-C2 on the expression of apoptosis-associated proteins were examined by Western blot. Apoptotic induction in MCS-C2-treated A549 cells was associated with cytochrome c release from mitochondria, which in turn resulted in the activation of caspase-9 and -3 and the cleavage of poly(ADP-ribose) polymerase (PARP). Based on these results, we conclude that MCS-C2 is a candidate therapeutic agent for the treatment of human lung cancer via upregulation and activation of p53.

• The Journal of Internal Korean Medicine
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• v.36 no.1
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• pp.13-21
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• 2015

Objectives : In Korea, Rhus verniciflua Stokes (RVS) has been used in traditional medicine for various diseases such as back pain, syndromes of the blood system in women, gastrointestinal disease, and cancer. However, the molecular mechanisms of its anti-cancer activity have not been clearly elucidated yet. Methods : This study investigated the possible mechanisms by which RVS extract (RVE) exerts its anti-proliferative action in cultured human breast carcinoma MCF-7 cells. Results : Treatment with RVE in MCF-7 cells resulted in inhibition of cell viability through G1 arrest of the cell cycle and induction of apoptosis in a time- and concentration-dependent manner, as determined by MTT assay and flow cytometry analysis. The induction of G1 arrest by RVE treatment was associated with the inhibition of cyclin D1, cyclin-dependent kinase (Cdk) 2, retinoblastoma protein (pRB), and mouse double minute 2 (MDM2) expression. Moreover, RVE treatment concentration dependently increased the levels of tumor suppressor p53, which was associated with the marked induction of Cdk inhibitors such as p21 (Waf1/Cip1) and p27 (Kip1). However, the inhibition of p53 function by the wild-type p53-specific inhibitor, pifithrin-α, abolished the above-mentioned effects of RVE, showing that p53 was responsible for the cytotoxicity of RVE Conclusions : These data indicate that a molecular pathway involving p53-dependent G1 cell cycle arrest plays a pivotal role in the cellular response to RVE, and demonstrate the potential applications of RVE as an anti-cancer drug for breast cancer treatment.

• Korean Journal of Medicinal Crop Science
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• v.26 no.5
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• pp.362-369
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• 2018

Background: For stable induction of tetraploidy in Fallopia multiflora Haraldson, colchicine was treated to establish the condition of induction and investigated the morphological and cytogenetic traits of the tetraploid plants obtained compared to those of diploid ones. Methods and Results: For the induction of tetraploidy, F. multiflora plants were soaked in aqueous solutions of colchicine at various concentration (0.1, 0.5, and 1.0%). After this, 2% dimethyl sulfoxide (DMSO) was added at room temperature on a shaker set at 150 rpm for periods of 12, 24, and 48 h. The induction rate of tetraploids appeared to be the highest in plants treated with 0.5% colchicine for 24 h. As the colchicine concentration and soaking time increased above these levels, the growing tip of the roots did not develop and they began to rot. When compared to diploid plants, tetraploids differed greatly in various characteristics, including the sizes and shapes of the leaves, fruits, flowers and roots. The induced tetraploid F. multiflora had larger guard cells, and chloroplasts, increased number of chloroplast in the guard cells and decreased stomatal densities. Conclusions: When colchicine induced plants for tetraploid, it can be distinguished from diploids, in various characteristics such as morphological changes as stomatal size, number of chloroplasts per guard cell, number of chromosomes and flow cytometry. Therefore, it proved that these methods are suitable, quick and easy methods for the identification of the ploidy level of F. multiflora.

• Journal of Power System Engineering
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• v.12 no.1
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• pp.13-19
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• 2008

In this study, a variable induction and exhaust system is applied to turbocharged diesel engine to improve the volumetric efficiency, especially, in a low and transient engine speed range where much of the pollutant matters are expelled out. The volumetric efficiency is known as one of the most important factor which affects significantly engine performance, fuel economy and further emission and noise level. As the torque increase with the engine speed up, the gas flow in an exhaust pipe become pulsating and then has an effect on boost up capacity of air charging into the cylinder and expelling capacity to atmosphere simultaneously. But at a low and idling speed, the pulsation effect was not so significant. Accordingly, resonator was employed to compensate their loss. The variable induction system consists of the secondary pipe, resonator, intercooler, and torque variance were examined with extended operating conditions. In the mean time, for interpretation and well understanding for the phenomena of wave action that arising during intake and exhaust process between turbocharger and variable intake system, the concept of the combined supercharging was introduced. Some of results are depicted which deal with a pressure history during valve events of induction process. Consequently, by the governing of these phase and amplitude of pulsating wave, it enables us to estimate and evaluate for the intake system performance and also, designing stage of the system layout.

• Geophysics and Geophysical Exploration
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• v.10 no.4
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• pp.275-284
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• 2007

For precise interpretation of magnetotelluric (MT) data distorted by irregular surface terrain, topography effects are investigated by computing apparent resistivities, phases, tippers and induction vectors for a three-dimensional (3D) hill-and-valley model. To compute MT responses for the 3D surface topography model, we use a 3D MT modeling algorithm based on an edge finite-element method which is free from vector parasites. Distortions on the apparent resistivity and phase are mainly caused by distorted currents that flow along surface topography. The distribution of tipper amplitudes over both hill and valley are the same, while the tipper points toward the center of hill and the base of the valley. The real part of induction vector also points in the same direction as that of tipper, while the imaginary part in the opposite direction.

• The Korean Journal of Physiology and Pharmacology
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• v.24 no.6
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• pp.517-527
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• 2020

Layer 2/3 pyramidal neurons (L2/3 PyNs) of the cortex extend their basal dendrites near the soma and as apical dendritic tufts in layer 1, which mainly receive feedforward and feedback inputs, respectively. It is suggested that neuromodulators such as serotonin and acetylcholine may regulate the information flow between brain structures depending on the brain state. However, little is known about the dendritic compartment-specific induction of synaptic transmission in single PyNs. Here, we studied layer-specific serotonergic and cholinergic induction of long-term synaptic plasticity in L2/3 PyNs of the agranular insular cortex, a lateral component of the orbitofrontal cortex. Using FM1-43 dye unloading, we verified that local electrical stimulation to layers 1 (L1) and 3 (L3) activated axon terminals mostly located in L1 and perisomatic area (L2/3). Independent and AMPA receptor-mediated excitatory postsynaptic potential was evoked by local electrical stimulation of either L1 or L3. Application of serotonin (5-HT, 10 μM) induced activity-dependent longterm depression (LTD) in L2/3 but not in L1 inputs. LTD induced by 5-HT was blocked by the 5-HT₂ receptor antagonist ketanserin, an NMDA receptor antagonist and by intracellular Ca²⁺ chelation. The 5-HT₂ receptor agonist α-me-5-HT mimicked the LTD induced by 5-HT. However, the application of carbachol induced muscarinic receptor-dependent LTD in both inputs. The differential layer-specific induction of LTD by neuromodulators might play an important role in information processing mechanism of the prefrontal cortex.

• Journal of Electrical Engineering and Technology
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• v.9 no.5
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• pp.1511-1519
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• 2014

The objective of this paper is to propose a modelling of a small compressed air energy storage system, which drives an induction generator based on a field-oriented control (FOC) principle for a renewable power generation. The proposed system is a hybrid technology of energy storage and electrification, which is developed to use as a small scale of renewable energy power plant. The energy will be transferred from the renewable energy resource to the compressed air energy by reciprocating air compressor to be stored in a pressurized vessel. The energy storage system uses a small compressed air energy storage system, developed as a small unit and installed above ground to avoid site limitation as same as the conventional CAES does. Therefore, it is suitable to be placed at any location. The system is operated in low pressure not more than 15 bar, so, it easy to available component in country and inexpensive. The power generation uses a variable speed induction generator (IG). The relationship of pressure and air flow of the compressed air, which varies continuously during the discharge of compressed air to drive the generator, is considered as a control command. As a result, the generator generates power in wide speed range. Unlike the conventional CAES that used gas turbine, this system does not have any combustion units. Thus, the system does not burn fuel and exhaust pollution. This paper expresses the modelling, thermodynamic analysis simulation and experiment to obtain the characteristic and performance of a new concept of a small compressed air energy storage power plant, which can be helpful in system designing of renewable energy electrification. The system was tested under a range of expansion pressure ratios in order to determine its characteristics and performance. The efficiency of expansion air of 49.34% is calculated, while the efficiency of generator of 60.85% is examined. The overall efficiency of system of approximately 30% is also investigated.