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http://dx.doi.org/10.5352/JLS.2013.23.6.832

Induction of G2/M Cell Cycle Arrest by Glutamine Deprivation in Human Prostate Carcinoma PC3 Cells  

Shin, Dong Yeok (Dongnam Institute of Radiological & Medicine Sciences)
Choi, Sung Hyun (Department of System Management, Korea Lift College)
Park, Dong Il (Department of Internal Medicine, Dongeui University College of Oriental Medicine)
Choi, Yung Hyun (Department of Biochemistry, Dongeui University College of Oriental Medicine)
Publication Information
Journal of Life Science / v.23, no.6, 2013 , pp. 832-837 More about this Journal
Abstract
In this study, it was investigated the possible mechanisms by which glutamine deprivation exerts its anti-proliferative action in cultured human prostate carcinoma PC3 cells. Glutamine deprivation resulted in inhibition of growth and G2/M arrest of the cell cycle in a time-dependent manner without apoptosis induction, as determined by MTT assay, DAPI staining and flow cytometry analyses. The induction of G2/M arrest by glutamine deprivation was associated with the inhibition of expression of Cdc2, cyclin A and cyclin B1, and up-regulation of the expression of cyclin-dependent kinase (Cdk) inhibitor p21(WAF1/CIP1) in both transcriptional and translational levels. Moreover, glutamine deprivation increased the phosphorylation of checkpoint kinase (Chk)1 and Chk2; however, the levels of Cdc25C phosphorylation were decreased in response to glutamine deprivation in a time-dependent manner. Our data provide a first biochemical evidence that glutamine deprivation suppresses cell viability through G2/M phase arrest without induction of apoptosis in PC3 cells.
Keywords
Glutamine deprivation; PC3 cells; cell cycle arrest;
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