• Title/Summary/Keyword: flow cell

Search Result 3,119, Processing Time 0.028 seconds

FLOW CYTOMETRIC ANALYSIS OF LYMPHOCYTE AND CYCLING CELL DISTRIBUTION IN PERIAPICAL LESIONS (유세포 분석기를 이용한 치근단 병소의 임파구 조성 및 CYCLING CELL 분포에 관한 연구)

  • Oh, Tae-Seok;Lim, Sung-Sam
    • Restorative Dentistry and Endodontics
    • /
    • v.18 no.2
    • /
    • pp.317-340
    • /
    • 1993
  • This study was designed 1) to compare the distributions of periapical inflammatory cells and 2) to identify lymphocytes and compare the lymphocyte distribution with T lymphocyte subpopulation and then 3) to examine the distribution of cycling cell in human dental periapical lesions. From each of the twenty-five human dental periapical lesions observed one small portion was fixed, embeded in paraffin, sectioned serially and stained with HE. The periapical inflammatory cells were counted to obtain the relative concentration of lymphocyte, plasma cell, macrophage and neutrophil. The large part of each lesion was analysed using Flow cytometer and monoclonal antibodies to obtain the relative concentration of T lymphocyte, B lymphocyte, T'helper cell and T suppressor/cytotoxic cell. In addition to that, seven human dental periapical lesions were examined with DNA analysis to observe the distribution of cycling cell. Following results were obtained: 1. 24 cases of the 32 periapical lesions examined were diagnosed as periapical granuloma and the remaining 8 cases as periapical cyst. Lymphocytes comprised 42.1% of total inflammatory cells in periapical granuloma and 41.8% in periapical cyst. Corresponding percentages for macrophages were 33.8% and 30.3%; for plasma cells, 15.9% and 19.0%; for neutrophils, 8.2% and 8.8%. 2. All of the periapical lesions examined had T lymphocyte, B lymphocyte, T helper cell, T suppressor/cytotoxic cell. And in all cases, T lymphocytes were observed predominantly more than B lymphocytes. 3. In 2 cases of the control group only T lymphocytes were found, and in the remaining 2 cases T lymphocytes were observed predominantly. 4. T helper cells were observed predominantly more than T suppressor/cytotoxic cells in all cases of perapical granulomas. 5. T suppressor/cytotoxic cells were observed predominantly more than T helper cells in 4 cases of periapical cysts (total 5 cases were examined) and only in one case T helper cells were more than T suppressor/cytotoxic cells. 6. In control group, T helper cells were predominant in 2 cases and T helper cells were equivalent to T suppressor/cytotoxic cells in one case. In remaining one case T suppressor/cytotoxic cells were predominant. 7. As the result of DNA analysis, the average proliferating indices of the various groups examined were measured as follows: in the control group 5.45%, in periapical granuloma 6.64%, in periapical cyst 10.1%. The highest index was observed in periapical cyst.

  • PDF

A Study on the Cell Structure for Capacitive Deionization System (축전식 탈염 시스템을 위한 셀 구조에 관한 연구)

  • Lee, Ju-Young;Seo, Seok-Jun;Park, Jung-Woo;Moon, Seung-Hyeon
    • Korean Chemical Engineering Research
    • /
    • v.48 no.6
    • /
    • pp.791-794
    • /
    • 2010
  • This study presents channel design of a CDI stack to achieve high removal efficiency in a large scale by applying parallel flow structure with a concentrated stream. The flow pattern in the stack was simulated by COMSOL Multiphysics program. To prove the salt removal performance, a unit cell and 20 cell stacks were tested at a flow rate condition of 18 ml/min and 360 ml/min, respectively. The removal efficiencies of the unit cell and the 20 cell stacks were obtained as 70.8 % and 75.6 %, respectively, with 100 mg/L sodium chloride solution. During the operation of cell test, water pressures of unit cell and 20 cell pair stack maintained in the ranges of 1.1 psi and 1.3~1.5 psi, respectively. It was demonstrated that the parallel cell structure with two concentrated streams can be employed in a large scale CDI for salt removal.

Molecular Biological Study of Anti-cancer Effects of Bee Venom on Human Melanoma Cell (약침용봉독액(藥鍼用蜂毒液)이 흑색종세포(黑色腫細胞)에 미치는 항암효과(抗癌效果)에 대(對)한 분자생물학적(分子生物學的) 연구(硏究))

  • Park, Chan-Yol;Nam, Sang-Soo;Kim, Chang-Hwan;Lee, Jae-Dong;Kang, Sung-Keel;Lee, Yun-Ho;Ahn, Byoung-Choul
    • Journal of Acupuncture Research
    • /
    • v.17 no.2
    • /
    • pp.169-186
    • /
    • 2000
  • To study anti-cancer effect and molecular biological mechanism of bee venom for aqua-acupuncture, the effects of bee venom on cell viability, apoptosis, and cell cycle were analyzed using MTT assay, tryphan blue assay, [3H]thymidine release assay, flow cytometric analysis, activity of caspase-3 protease activity assay, and immunocytometric analysis of PCNA. To explore whether anti-cancer effects of bee venom are associated with the transcriptional control of gene expression, quantitative RT-PCR analysis of apoptosis- and cell cycle-related genes was performed. The obtained results are summarized as follows: 1. The MTT assay demonstrated that cell viability was decreased by bee venom in a dose-dependant manner. 2. Significant induction of apoptosis was identified using tryphan blue assay, [$^3H$]thymidine release assay, and flow cytometric analysis of sub $G_1$ fraction. 3. In analysis of caspase-3 protease activity, the activity had increased significantly, in a dose-dependant manner. 4. Quantitative RT-PCR analysis of the apoptosis-related genes showed that Bcl-2 and $Bcl-X_L$ were down-regulated whereas Bax was up-regulated by bee venom treatment. 5. In flow cytometric analysis of cell cycle and immunocytometric analysis of PCNA expression, cell numbers of $G_1$ phase was increased by a dose-dependant manner. 6. In quantitative RT-PCR analysis of the cell cycle-related genes, p21, p27, and p57 were increased, while Cyclin D1, CDK4, c-Myc, c-Fos, and Histone H3 were decreased. In contrast, there were no remarkable changes in expression levels of CDC2 and c-Jun.

  • PDF

A NEW PRESSURE GRADIENT RECONSTRUCTION METHOD FOR A SEMI-IMPLICIT TWO-PHASE FLOW SCHEME ON UNSTRUCTURED MESHES (비정렬 격자 기반의 물-기체 2상 유동해석기법에서의 압력기울기 재구성 방법)

  • Lee, H.D.;Jeong, J.J.;Cho, H.K.;Kwon, O.J.
    • Journal of computational fluids engineering
    • /
    • v.15 no.2
    • /
    • pp.86-94
    • /
    • 2010
  • A thermal-hydraulic code, named CUPID, has been developed for the analysis of transient two-phase flows in nuclear reactor components. A two-fluid three-field model was used for steam-water two-phase flows. To obtain numerical solutions, the finite volume method was applied over unstructured cell-centered meshes. In steam-water two-phase flows, a phase change, i.e., evaporation or condensation, results in a great change in the flow field because of substantial density difference between liquid and vapor phases. Thus, two-phase flows are very sensitive to the local pressure distribution that determines the phase change. This in turn puts emphasis on the accurate evaluation of local pressure gradient. This paper presents a new reconstruction method to evaluate the pressure gradient at cell centers on unstructured meshes. The results of the new scheme for a simple test function, a gravity-driven cavity, and a wall boiling two-phase flow are compared with those of the previous schemes in the CUPID code.

IMPROVEMENT OF A SEMI-IMPLICIT TWO-PHASE FLOW SOLVER ON UNSTRUCTURED MESHES (비정렬 격자계에서의 물-기체 2상 유동해석코드 수치 기법 개선)

  • Lee, H.D.;Jeong, J.J.;Cho, H.K.;Kwon, O.J.
    • 한국전산유체공학회:학술대회논문집
    • /
    • 2010.05a
    • /
    • pp.380-388
    • /
    • 2010
  • A thermal-hydraulic code, named CUPID, has been developed for the analysis of transient two-phase flows in nuclear reactor components. A two-fluid three-field model was used for steam-water two-phase flows. To obtain numerical solutions, the finite volume method was applied over unstructured cell-centered meshes. In steam-water two-phase flows, a phase change, i.e., evaporation of condensation, results in a great change in the flow field because of substantial density difference between liquid and vapor phases. Thus, two-phase flows are very sensitive to the local pressure that determines the phase change. This in turn puts emphasis on the accurate evaluation of local pressure gradient. This paper presents a new numerical scheme to evaluate the pressure gradient at cell centers on unstructured meshes. The results of the new scheme for a simple test function a gravity-driven cavity, and a wall boiling two-phase flow are compared with those of the previous schemes in the cupid code.

  • PDF

Analytical Application of Glow Discharge Atomic Absorption Spectroscopy (GD-AAS) Using Three Types of Jet Configurations Under Power Mode

  • Hwang, Jun Ho;Lee, Ki Beom;Kim, Min Su;Lee, Seong Ro;Kim, Hasuck;Kim, Hyo Jin;Lee, Gae Ho
    • Analytical Science and Technology
    • /
    • v.8 no.4
    • /
    • pp.443-448
    • /
    • 1995
  • Three anode configurations of six-jet, cone-jet and cylindrical-jet are tested for their analytical performance under power mode operation. The effect of pressure, power and gas flow rate on atomic absorption signals have been studied. The increase of atomic absorption signal of sample element is observed at a fixed pressure in all configurations as the gas flow rate increase up to 300-600 seem, and as the power dissipated in the glow discharge cell increase. The lower the pressure is in the glow discharge cell at a fixed discharge power and argon flow rate, the greater the absorbance of sample element is. The optimum conditions are taken from these data and a calibration curve of Cu in low-alloy steel sample is obtained. In this calibration curve, six-jet configuration shows the best analytical results varies as the sample element.

  • PDF

Development of Low Dissipative AUSM-type Scheme (Low Dissipative AUSM-type 수치기법 개발)

  • Kim, Kyu-Hong
    • Journal of the Korean Society for Aeronautical & Space Sciences
    • /
    • v.32 no.9
    • /
    • pp.12-26
    • /
    • 2004
  • A new treatment of cell-interface flux in AUSM-type methods is introduced to reduce the numerical dissipation. Through analysis of TVD limiters, a criterion for the more accurate prediction of cell-interface state is found out and M-AUSMPW+ is developed by determining the transferred property newly and appropriately within the criterion. The superiority of M-AUSMPW+ is clearly revealed in multi-dimensional flow problems. It can eliminate numerical dissipation effectively in a non-flow aligned grid system. As a result, M-AUSMPW+ is shown to be much more accurate and effective than other previous schemes in multi-dimensional problems. Through a stationary contact discontinuity, a vortex flow, a shock wave/boundary layer interactions and viscous shock tube problems, it is verified that accuracy of M-AUSMPW+ is improved.

Effect of Anodic Gas Compositions on the Overpotential in a Molten Carbonate Fuel Cell

  • Lee C.G.;Kim D.H.;Hong S.W.;Park S.H.;Lim H.C.
    • Journal of the Korean Electrochemical Society
    • /
    • v.9 no.2
    • /
    • pp.77-83
    • /
    • 2006
  • Anodic overpotential has been investigated with gas composition changes in a $100cm^2$ class molten carbonate fuel cell. The overpotential was measured with steady state polarization, reactant gas addition (RA), inert gas step addition (ISA), and electrochemical impedance spectroscopy (EIS) methods at different anodic inlet gas compositions, i.e., $H_2:CO_2:H_2O=0.69:0.17:0.14\;atm\;and\;H_2:CO_2:H_2O=0.33:0.33:0.33\;atm$, at a fixed $H_2$ flow rate. The results demonstrate that the anodic overpotential decreases with increasing $CO_2\;and\;H_2O$ flow rates, indicating the anode reaction is a gas-phase mass-transfer control process of the reactant species, $H_2,\;CO_2,\;and\;H_2O$. It was also found that the mass-transfer resistance due to the $H_2$ species slightly increases at higher $CO_2\;and\;H_2O$ flow rates. EIS showed reduction of the lower frequency semi-circle with increasing $H_2O\;and\;CO_2$ flow rate without affecting the high frequency semi-circle.