• Title/Summary/Keyword: flavonoid glycosides

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Phenolic Composition and Antioxidant Activities of Different Solvent Extracts from Pine Needles in Pinus Species

  • Kang, Yoon-Han;Howard, Luke R.
    • Preventive Nutrition and Food Science
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    • v.15 no.1
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    • pp.36-43
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    • 2010
  • The purpose of this study was to investigate the phenolic acid, proanthocyanidin (PAs), and flavonol glycoside contents, as well as the antioxidant activities of pine needle extracts from six species of young pine trees. The extracts were prepared from Section Pinus (Diploxylon): P. densiflora, P. sylvestris, P. pinaster and P. pinea, and Section Strobus (Haploxylon): P. koraiensis and P. strobus. Phenolics were extracted from pine needles with 80% acetone to obtain the soluble free fraction, and insoluble residues were digested with 4 M NaOH to obtain bound ethyl acetate and bound water fractions. Phenolics were analyzed by HPLC, and the hydrophilic antioxidant activity was measured using oxygen radical absorbance capacity (ORAC). Total phenolic and flavonoid contents of the soluble free fraction were higher than those of the bound ethyl acetate and bound water fractions. The main phenolics were monomers and polymers of PAs in the soluble free fraction, and phenolic acids and flavonol glycosides in bound ethyl acetate fraction. Flavonol glycosides found in different species of pine needles were qualitatively similar within fractions, but composition varied among Pinus sections. High levels of kaempferol arabinoside and an unknown compound were present in all Strobus species. The soluble free fraction had the highest antioxidant activity, followed by bound ethyl acetate and bound water fractions.

Identification and Quantitative Analysis of Flavonol Glycosides from Ginkgo biloba Leaves by High Performance Liquid Chromatography (고속액체크로마토그라피에 의한 은행잎중 Flavonoid Glycoside의 확인 및 정량)

  • Kang, Sam-Sik;Kim, Ju-Sun;Kwak, Wie-Jong;Kim, Ki-Hyup
    • Korean Journal of Pharmacognosy
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    • v.21 no.2
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    • pp.148-152
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    • 1990
  • Seven flavonol glycosides from the EtOAc fraction of Ginkgo biloba leaves were identified by high performance liquid chromatography. Separation by reversed phase chromatography on $Lichrosorb^{\circledR}$ RP-18 column was achieved by isocratic elution. The content of the major acylated flavonol glycoside, kaempferol 3-0-[$6'-O-{p}-coumaroyl-{\beta}-_D-glucosyl(1{\rightarrow}2)-{\alpha}-_L-rhamnoside$] was about 8.0% and 0.55% for EtOAc fraction and MeOH extract, respectively.

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Two new megastigmane glycosides from Phyllanthus ussuriensis

  • Ryu , Min-Youl;Kim, Chul-Young;Kim, Jin-Woong
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.191.2-191.1
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    • 2003
  • Phyllanthus ussuriensis Rupr. et Maxim. (Euphorbiaceae) has long been used in folk medicine to treat kidney and urinary bladder disturbances, intestinal infections, diabetes, and hepatitis. Reported chemical constituents of this species are one flavonoid (rutin), two gallotannins (gallic acid, methyl gallate) and two ellagitannins.An investigation of the n-BuOH fraction of P. ussuriensis led to the isolation of two new megastigmane glycosides, 10-hydroxy-4,7-megastigmadiene-3-one-9-O-b-D-glucopyranoside (1), 10- hydroxy-4,6-megastigmadiene-3-one-9-O-b-D-glucopyranoside (2) and two known compounds roseoside (3) and 3-oxo-a-ionol-9-O-b-D-glucopyranoside (4). (omitted)

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Studies on the Constituents of the Herbs of Ajuga multiflora (I)

  • Yu, Young-Jun;Do, Jae-Chul;Jung, Keun-Young;Kwon, Soon-Youl;Son, Kun-Ho
    • Korean Journal of Pharmacognosy
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    • v.29 no.2
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    • pp.75-78
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    • 1998
  • In the course of phytochemical studies for the aerial parts of Ajuga multiflora, one flavonoid and two iridoid glycosides were isolated and identified as apigenin (1), 8-O-acetylharpagide (2) and harpagide (3) on the basis of spectroscopic evidence.

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Flavonoids from the Leaves of Alnus hirsuta (물오리나무 잎의 플라보노이드 화합물)

  • 이민원;정동욱;이연아;박명신;도상학
    • YAKHAK HOEJI
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    • v.43 no.5
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    • pp.547-552
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    • 1999
  • Phytochemical examination of the leaves of Alnus hirsuta, one of the indigenous species grows in Korea, has led to the isolation of one flavan 3-ol and two flavonol glycosides. Structures of these compounds are identified as (-)-epicatechin (1), quercetin $3-O-{\beta}-D-galactopyranoside$ (2), quercetin $3-O-{\beta}-D-galactopyranoside$ (3) by means of physico-chemical and spectral evidences.

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Isolation of Ginkgolides and the Effect of These Components on Inflammation in Mice Induced by Complete Freund's Adjuvant.

  • Lee, Soon-Hyun;Han, Yong-Moon
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.137.3-138
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    • 2003
  • The major active components of EGb 761, extract of Ginkgo biloba leaves, include flavonoid glycosides and unique diterpenes known as ginkgolides. Ginkgolides are potent inhibitors of platelet activating factor. In this study, we investigated antiinflammatory activity of ginkgolides on the Complete Freund"s Adjuvant (CFA)-induced mice. The ginkgolides were extracted from commercially available EGb 761. This extracting procedure was done by sequential treatments of the EGb 761 with chloroform, methanol, and water. (omitted)tted)

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Saponins and Flavonoid Glycosides from Yellow Sweetclover

  • Kang, Sam-Sik;Lee, Young-Soon;Lee, Eun-Bang
    • Archives of Pharmacal Research
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    • v.11 no.3
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    • pp.197-202
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    • 1988
  • A new saponin, 3-O-[${\alpha}$-L-rhamnopyranosyl$(1{\rightarrow}2)$-${\beta}$-D-glucopyranosyl$(1{\rightarrow}2)$-${\beta}$-D-glucuronopyranosyl]soyasapogenol B carboxylate (6) has been isolated from the medicinal plant yellow sweetclover together with azukisaponin II(7), robinin(8), and clovin(9). 7,8, and 9 are reported for the first time from this plant. The new saponin(6) exhibited inhibitory action on leucocyte migration in inflammation.

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