• Journal of Aquaculture
• /
• v.10 no.2
• /
• pp.171-178
• /
• 1997

Infectious pancreatic necrosis virus (IPNY) is an economically important fish pathogen since it causes the high-mortality disease in early stage of hatchery-reared fishes. In order to develop a rapid, sensitive and highly specific detection method for IPNV, reverse transcription-polymerase chain reaction (RT-PCR) was carried out using the oligonucleotide primers selected from the sequence of VP2, a major capsid polypertide of IPNV. As little as 40ng of purified IPNV dsRNA was detected by RT-PCR amplification, but no amplification products were obtained when nucleic acid genomes from other fish pathogens such as IHNV were used as RT-PCR templates. in situ RT-PCR methods are useful for the rapid and sensitive identification of IPNV.

• Journal of Aquaculture
• /
• v.21 no.3
• /
• pp.196-200
• /
• 2008

Korean spotted barbel Hemibarbus mylodon, is an endangered and endemic freshwater species in the Korean peninsula. Amplified fragment length polymorphism (AFLP) was used to analyze the genetic diversity and population genetic structure of three populations (Bukhan, Namhan and Imjin-river). Fifteen AFLP primer pairs produced 795 products of which 135 were polymorphic(17%). Percentages of polymorphic bands were similar among the three populations, with accounting 11.9%, 11.1%, and 13.4% for Bukhan, Namhan and Imjin-river populations, respectively. An average genetic similarity among the three populations was 0.969. The average heterozygosity (0.033-0.040) and genetic diversity (0.036-0.043) were significantly low. Pairwise distance and fixation index analyses of three populations also suggested quite a low genetic differentiation one another. These results would provide a fundamental baseline data to develop the effective strategy for the management and restoration of this endangered fish species.

• Food Science of Animal Resources
• /
• v.33 no.2
• /
• pp.214-220
• /
• 2013

The effects of different cations on its composition and flavor characteristics of pidan white and yolk produced with duck egg in comparison to its fresh egg were investigated. Mineral content such as calcium, magnesium, sodium and potassium were significantly increased in pidan yolk irrespective of its cations in pickle solution in comparison to the fresh yolk (P<0.05). It confirmed the migration of minerals from the pickling solution to the egg. However, calcium and magnesium was found lower in 0.2% $PbO_2$ treated pidan. Less pidan flavor compounds were generated in pidan white produced with the aid of 0.2% $PbO_2$. It confirmed that binding of lead prevent the maillard reaction in the pidan treated with $PbO_2$. Benzaldehyde, ketones, alcohol and acid found in the pidan white treated with 0.2% $ZnCl_2$ reveals that volatiles are generated most likely from maillard reaction. However, pidan yolk of both 0.2% $PbO_2$ and 0.2% $ZnCl_2$ showed higher generation of volatiles more likely from yolk lipids. Butanal, pentanal, hexanal, and heptanal are of those aldehydes found in 0.2% $ZnCl_2$ treated pidan yolk whereas hexanal is the only aldehyde detected in 0.2% $PbO_2$ treated pidan yolk. Thus, cations in the pickling solution affect the flavor characteristics of pidan white and yolk.

• Korean journal of applied entomology
• /
• v.50 no.3
• /
• pp.247-251
• /
• 2011

Wood products are sometimes infested with cossonid weevils. A cossonid weevil, Hexarthrum brevicorne Wollaston has been found on wood boards used for printing books in the Jeonju Confucian temple and school (2004), a wood cabinet in the Museum of Milyang (2007), a wood wardrobe in the Museum of Seoul (2008) and on wood boards used for printing Buddhist scriptures in the temple of Suncheon (2008). Wood utensils for living in the Museum of Seoul were found to be infested with another cossonid weevil, Rhyncolus sculpturatus (Waltl) in 2008. To protect the cultural property from insect pests in the field of conservation science, more comprehensive insect pest management (IPM) programs are required.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.17 no.2
• /
• pp.135-140
• /
• 2017

The demand for aquatic products in the domestic and overseas is increased, so that the aquaculture industry can achieve high performance by controlling and standardizing the production even with a relatively small amount of resources compared with existing fisheries. However, traditional method has problems of low productivity such as natural disasters and ecosystem pollution, and it is necessary to develop a new culture system that can move to the optimal culture site. In order to find the optimal location, you need to collect and analyze the necessary data such as temperature and DO in real time. Data analysis was performed by using K-means clustering method based on machine learning, so that it was possible to decision when and where to move the farm by repeated unsupervised learning. The proposed research could solve the problems of low productivity such as natural disasters and ecosystem pollution if applied to regressive fish farmers.

• Korean Journal of Plant Resources
• /
• v.30 no.3
• /
• pp.272-279
• /
• 2017

EP was obtained through 20% ethanol extraction of Pueraria lobata root, and the fermented form of EP, FEP, was prepared from the EP after incubating with Lactobacillus rhamnosus vitaP1. There was no significant toxicity by EP and FEP up to $1000{\mu}g/ml$ in NIH-3T3, HaCaT, and B16F10 cells. In addition to antioxidant potentials of EP and FEP determined by DPPH and ABST assays, we confirmed increase of procollagen type I and elastin synthesis by supplementation of the EP and FEP at the concentration of $50{\mu}g/ml$ using ELISA kits. The protein expression levels of matrix metalloprotease (MMP)-1, -3, and -9, those are involved in the degradation of collagen or other skin matrix proteins, were remarkably suppressed while their inhibitory protein metallopeptidase inhibitor 1 (TIMP-1) was greatly up-regulated by supplementation of the EP and FEP at a concentration of $50{\mu}g/ml$. Taken together, both EP and FEP supplementation could be involved in the suppression of the skin wrinkle formation through inhibiting degradation of collagen and stimulating the synthesis of collagen and elastin. The results showed that the anti-wrinkle potential of the EP and FEP will be a promising candidate for developing cosmeceutical compounds or products.

• Journal of the Korean Chemical Society
• /
• v.5 no.1
• /
• pp.42-47
• /
• 1961

A titration of a small amount of iron with standard potassium ferrocyanide using potassium thiocyanate as indicator has been studied. A sample solution containing $0.1{\sim}1.0$ mg. $Fe^{3+}$ in 60 ml. is pipeted into 100 ml. Erlenmyer flask and the pH of the solution is adjusted to $1.5{\sim}3.0$ with 0.1 N or 1 N $HNO_3$ and $NH_4OH.$ To this solution one ml. of 1 M KCNS solution as indicator is added. The solution colored by iron thiocyanate complex is titrated with 1/200 M or 1/400 M standard solution of potassium ferrocyanide from a 5 ml. micro-buret. Near the end point, when the color of sample changes from deep red to green, about 20 ml. of ether is added and shake the flask vigorously. The red color is extracted to the ether layer. To settle the ether layer a few drops of ethanol is added and then standard solution is added dropwise and shake vigorously. The end point is reached when the color of the ether layer disappears owing to the quantitative formation of $Fe_4[Fe(CN)_6]_3.$ In this titration, 0.lmg. of $Fe^{3+}$ can be determined within 1.0% of titration error, provided the following optimum conditions, i.e., pH $1.5{\sim}3.0$, final concentration of KCNS indicator; $0.01{\sim}0.02M$, at room temperature. The titration found to be interfered by the presence of slightly soluble salts, stable complex forming ions and the ions which would be reduced by ferrocyanide or oxidized by ferric ion.

• 한국지구물리탐사학회:학술대회논문집
• /
• 1999.08a
• /
• pp.1-20
• /
• 1999

In Japan, geophysical exploration methods have been widely applied to civil engineering and construction fields for a long time. In particular, seismic refraction has long played a significant role in geotechnical site investigations for tunnels, dams and landslides. However, our growing interest on the social and natural environment makes the methods available and its application fields diversify. Digital technologies such as personal computer have revolutionized our ability to acquire large volumes of data rapidly, and to produce more reliable results for subsurface image. Also, color graphics easily visualizes survey results In a more understandable manner, These days geophysical methods are essential to assessing grouting effects, predicting the front of tunnel cutting face, monitoring the movement, pollution and purification process of groundwater. Now three-dimensional exploration techniques have developed for the site characterization in civil engineering and construction needs.

• Food Science of Animal Resources
• /
• v.39 no.1
• /
• pp.84-92
• /
• 2019

Listeria monocytogenes is a major cause of serious foodborne illness in the dairy foods. Although Caenorhabditis elegans model is well established as a virulence model of pathogenic bacteria, its application on L. monocytogenes is critically unclear. The objective of this study was to carry out an evaluation of L. monocytogenes toxicity using C. elegans nematode as a simple host model. We found that C. elegans nematodes have high susceptibility to L. monocytogenes infection, as a consequence of accumulation of bacteria in the worms' intestine. However, L. innocua, which is known to be non-toxic, is not accumulate in the intestine of worms and is not toxic similarly to Escherichia coli OP50 known as the normal feed source of C. elegans. Importantly, immune-associated genes of C. elegans were intensely upregulated more than 3.0-fold when they exposed to L. monocytogenes. In conclusion, we established that C. elegans is an effective model for studying the toxicity of L. monocytogenes and we anticipate that this system will result in the discovery of many potential anti-listeria agents for dairy foods.

• Korean Journal of Remote Sensing
• /
• v.38 no.6_2
• /
• pp.1653-1661
• /
• 2022

In this study, the classification of cold water and normal water based on Geo-Kompsat 2A images was performed. Daily mean surface temperature products provided by the National Meteorological Satellite Center (NMSC) were used, and convolution neural network (CNN) deep learning technique was applied as a classification algorithm. From 2019 to 2022, the cold water occurrence data provided by the National Institute of Fisheries Science (NIFS) were used as the cold water class. As a result of learning, the probability of detection was 82.5% and the false alarm ratio was 54.4%. Through misclassification analysis, it was confirmed that cloud area should be considered and accurate learning data should be considered in the future.