• 한국어병학회지
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• 제28권2호
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• pp.71-78
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• 2015

In this study, we conducted to the development of a rapid antimicrobial susceptibility test method using WST-1 which is known to water-soluble tetrazolium salt, in order to rapidly response against bacterial diseases in fish. Eight of antibiotics which are permissioned for marine organism from government were used to rapid antimicrobial susceptibility testing using the WST-1. As a result, a similar tendency was verified compare to conventional antibiotic susceptibility test results. Generally, the antibiotic susceptibility test method required about 3 days (72 hours) for determine the effective antibiotics, however, we have confirmed that the our method using WST-1 was required at least 36 hours in this study. Consequentially, our method will contribute to development of rapid antimicrobial susceptibility testing for bacterial diseases in fish.

• 수산해양교육연구
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• 제27권1호
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• pp.184-197
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• 2015

The purpose of this study is to analyze the trends that the Fishery and Shipping Department teachers at specialized vocational high schools have noticed about the characteristics of the applicant pool taking the College Scholastic Achievement Test(CSAT) since the vocational education division was first introduced in the CSAT in 2005. In addition, surveyed data from the study is highly desirable for researchers to understand the changes in the characteristics of the applicants for the fishery and maritime department in the CSAT. This study not only provides solid improvement on the selection of questions for the CSAT but also facilitates in the process of writing contemporary and relevant exam questions. The survey included 76 Teachers from 12 High School of Fisheries and Shipping Industry. The results were as follows. First, the reduction in the number of applicants for Fishery and Maritime department in the CSAT after 2010 is due to the new governmental policy that encourages students in vocational schools to find employment rather than pursuing further education. Second, the biggest group of students who forgo taking the CSAT after 2010 is those with high marks at school. Third, prediction of outcome after declining to take the person for the time being would be kept to a minimum sustained was staring personnel at some point.

• 수산해양기술연구
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• 제49권3호
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• pp.282-290
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• 2013

The global warming related to GHG (greenhouse gases) emissions from industries is a major issue globally. Furthermore, GHG emissions from the fishery industries also represent an important issue, as indicated by "The Code of Conduct for Responsible Fisheries" at the Cancun, Mexico, meeting in 1992 and by the Kyoto protocol in 2005. Korea pronounced itself to be a voluntary exclusion management country at the 16th IPCC at Cancun, Mexico, in 2010. However, few analyses of GHG emissions from Korean fisheries have been performed. Therefore, a quantitative analysis of GHG emissions from the major Korean fisheries is needed before guidelines for reducing GHG emissions from the fishing industry can be established. The aim of this study was to assess the present GHG emissions from the Korean Purse seine fishery using the LCA (life cycle assessment) method. The system boundary and allocation method were defined for the LCA analysis. The fuel consumption factor of the purse seine fishery was also calculated. The GHG emissions for the edible fish were evaluated by determining the weights of whole fish and gutted fish. Finally, the GHG emissions required to produce 1kg of whole fish and 1kg of edible fish were deduced. The results will help determine the GHG emissions from the fishery. They will also be helpful to stakeholders and the government in understanding the circumstances involved in GHG emissions from the fishing industry.

• Fisheries and Aquatic Sciences
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• 제25권5호
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• pp.264-275
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• 2022

A non-destructive environmental DNA protocol for the genetic analysis of sea cucumber (Apostichopus japonicus) resources DNA was established. Among the several commercial DNA extraction kits, the DNeasy^® Plant Mini Kit was selected as the best choice to obtain the high-quality genomic DNAs from the mucous sea cucumber. As the temperature and incubation time increased, the amount of extracted environmental DNA was also large, but it was judged that the increased amount did not affect as much as 2-3 times. Therefore, these conditions were not considered to be the main factors to consider in actual environmental DNA extraction. However, the amount of seawater relative to the size of the sample was judged as a major consideration, and a sufficient amount of environmental DNA for analysis was secured when stored within 1 min while stirring the volume of seawater corresponding to the total sea cucumber weight (g). In securing the environmental DNA of sea cucumbers, the mortality rate of sea cucumbers in all experiments was 0, and it was judged that the effects of sea cucumbers were not significant through this treatment. Through the results of this study, sea cucumber DNA research, which has been conducted in a destructive method, can be conducted non-destructively through environmental DNA analysis. Through this study, we have secured a standard protocol that can successfully extract the sea cucumber DNA through environmental DNA. It is not only excellent in terms of time and cost of traditional DNA analysis method currently used, but it is completely non-destructive in the ecosystem of the survey area. It is believed that the system can be transformed in a way that does not affect it. However, it is thought that various standard protocols should be established considering the characteristics of each type.

• Fisheries and Aquatic Sciences
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• 제25권3호
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• pp.151-157
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• 2022

Various approaches have been applied to transform aquaculture from a manual, labour-intensive industry to one dependent on automation technologies in the era of the fourth industrial revolution. Technologies associated with the monitoring of physical condition have successfully been applied in most aquafarm facilities; however, real-time biological monitoring systems that can observe fish condition and behaviour are still required. In this study, we used a video recorder placed on top of a fish tank to observe the swimming patterns of rock bream (Oplegnathus fasciatus), first one fish alone and then a group of five fish. Rock bream in the video samples were successfully identified using the you-only-look-once v3 algorithm, which is based on the Darknet-53 convolutional neural network. In addition to recordings of swimming behaviour under normal conditions, the swimming patterns of fish under abnormal conditions were recorded on adding an anaesthetic or lowering the salinity. The abnormal conditions led to changes in the velocity of movement (3.8 ± 0.6 cm/s) involving an initial rapid increase in speed (up to 16.5 ± 3.0 cm/s, upon 2-phenoxyethanol treatment) before the fish stopped moving, as well as changing from swimming upright to dying lying on their sides. Machine learning was applied to datasets consisting of normal or abnormal behaviour patterns, to evaluate the fish behaviour. The proposed algorithm showed a high accuracy (98.1%) in discriminating normal and abnormal rock bream behaviour. We conclude that artificial intelligence-based detection of abnormal behaviour can be applied to develop an automatic bio-management system for use in the aquaculture industry.

• 수산해양기술연구
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• 제47권3호
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• pp.214-221
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• 2011

This study was performed to develop a video based system which can be used to measure the averaged fish size in a non-intrusive fashion. The design was based on principles of simple stereo geometry, incorporated fish dimensions weight relationships and took into consideration fish movement to lower system costs. As the fish size is an important factor that impacts the economy of an aquaculture enterprise. Size measurements, including fork length, width or height, girth, thickness and mass, can be used to determine fish condition in the fish farm, so the averaged fish size of fish cage needs to consistently monitor in open ocean aquaculture cage. A precision of ${\pm}3%$ for replicate length measurements of a 60cm bar is obtained at distances between 2.0 and 6.0m, and the mean fork length and mean swimming speed of bluefin tuna were estimated to 48.8cm and 0.78FL/s, respectively.

• 수산해양기술연구
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• 제54권3호
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• pp.224-230
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• 2018

Squid is one of the important fisheries resources in Korea. Therefore, squid has been designated and managed as a target species of total allowable catch (TAC) since 2007, but the catch amount is gradually decreasing. The analysis was conducted to identify the change of relative fishing power index to develop the vessel and gear technology that may have improved the fishing efficiency of the offshore squid jigging fishery from 1960s to 2010s. Gross tonnage per fishing vessel increased with the increase in size until 1990, but then gradually decreased to 41.0 tons in 2000 and 37.1 tons in 2010. The illuminating power (energy consumption) by fishing lamps increased to 180 kW in 2005 and stabilized to 120 kW in 2015. Jigging machine started to be supplied to fishing vessels from the early 1970s, and fish finders began to be supplied in the early 1980s and gradually increased. Therefore, the relative fishing power index in the offshore squid jigging fishery increased from 1.0 in 1980 to 1.1 in 1990, to 3.5 in 2000 and to 2.5 in 2010, but the increment rate slowed down gradually. The results are expected to contribute to reasonable fisheries stock management.

• Fisheries and Aquatic Sciences
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• 제19권5호
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• pp.25.1-25.8
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• 2016

The fractionation of serine protease inhibitor (SPI) from fish roe extracts was carried out using polyethylene glycol-4000 (PEG4000) precipitation. The protease inhibitory activity of extracts and PEG fractions from Alaska pollock (AP), bastard halibut (BH), skipjack tuna (ST), and yellowfin tuna (YT) roes were determined against target proteases. All of the roe extracts showed inhibitory activity toward bromelain (BR), chymotrypsin (CH), trypsin (TR), papain-EDTA (PED), and alcalase (AL) as target proteases. PEG fractions, which have positive inhibitory activity and high recovery (%), were the PEG1 fraction (0-5 %, w/v) against cysteine proteases (BR and PA) and the PEG4 fraction (20-40 %, w/v) against serine proteases (CH and TR). The strongest specific inhibitory activity toward CH and TR of PEG4 fractions was AP (9278 and 1170 U/mg) followed by ST (6687 and 2064 U/mg), YT (3951 and 1536 U/mg), and BH (538 and 98 U/mg). The inhibitory activity of serine protease in extracts and PEG fractions from fish roe was stronger than that of cysteine protease toward common casein substrate. Therefore, SPI is mainly distributed in fish roe and PEG fractionation effectively isolated the SPI from fish roes.

• 수산해양기술연구
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• 제28권3호
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• pp.295-305
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• 1992

The critical strain energy release rate and the failure mechanisms of glass-carbon epoxy resin hybrid composites are investigated in the temperature range of the ambient temperature to 8$0^{\circ}C$. The direction of laminates and the volume fraction are [(+45, -45, 0, 0) sub(2) ] sub(s), 50%, respectively. The major failure mechanisms of these composites are studied using the scanning electron microscope for the fracture surface. Results are summarized as follows: 1) The critical strain energy release rate shows a maximum at ambient temperature and it tends to decrease as temperature goes up. 2) The critical strain energy release rate increases as the content of glass increases, and especially shows dramatic increase for the high glass fiber content specimens. 3) Major failure mechanisms can be classfied such as localized shear yielding, fiber-matrix debonding, matrix micro-cracking, and fiber pull-out and/or delamination.

• 수산경영론집
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• 제53권4호
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• pp.1-13
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• 2022

The introduction of the wholesalers system is being considered from various aspects in order to improve the problems of the listed auction system of wholesale corporations. The wholesalers system is a corporation that receives designation from the creator and purchases or consigns agricultural and fishery products to wholesale or broker sales, and has the advantage of reducing the distribution stage and distribution costs. The purpose of this study is to evaluate the wholesalers system introduced to improve the problems of the listed auction system of wholesale corporations through a case study of market wholesalers handling fishery products.