• Journal of Aquaculture
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• v.10 no.2
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• pp.153-161
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• 1997

This study was conducted to evaluate the possible utilization and the replacing range of leather meal (LM) or meat and bone meal (MBM) as a fish meal replacer for the dietary animal protein source of growing israeli carp, Cyprinus carpio. Five different diets were formulated on isonitrigenous and isocaloric basis of 38.5% curde protein and 15.3 KJ/g diet. Percentage of the graded levels of replacement of white fish meal (WFM) by LM/MBM on the basis of crude protein were at follows : diet 1, 100% WFM (control) ; diet 2, 75% WFM+25% LM ; diet 3, 50% LM ; diet 4, 75% WFM+25% MBM ; diet 5, 50 WFM+50% MBM and LM or MBM, and approximately 64.5% of plant protein. After one week of conditioning period, fish averaging 10g were divided into five groups and fed one of the experimental diets for 12 weeks. In the first and the second 4 weeks of feeding trials, there were no significant differences in weight gain between two groups of fish fed diet 1 (control) and 2 (P>0.05), while fish fed diet 3, 4 and 5 had a significantly lower body weight gain than fish fed diet 1 (P<0.05). In contrast to those two periods, all diet groups were not significantly different compared to diet 1 in body weight gain during the third 4 weeks of feeding trial (P<0.05). These results indicated that LM and MBM could be used as a fishmeal replacer up to 50% in growing israeli carp.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.5
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• pp.469-474
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• 2000

This study was conducted to investigate the utilization of defatted soybean meal as a substitute for fish meal in the diet for juvenile flounder (Paralichthys olivaceus). Duplicate groups of average weighing 35 g were fed one of four isonitrogenous ($54{\%}$) and isocaloric (575 kcal/100 g diet) diets containing $0{\%}, 10{\%}, 20{\%} and 30{\%}$ soybean meal for 45 days. Survival rates of all groups were $100{\%}$, Weight gain of fish decreased with increasing dietary soybean meal levels, However, this value was not significantly different between fish fed the control and $10{\%}$ soybean meal diet (P > 0.05). Feed efficiency and protein efficiency ratio decreased with increasing dietary soybean meal levels, but no significant differences were found among fish fed the control, $10{\%}\;and\;20{\%}$ soybean meal diets (P > 0.05). Daily feed and protein intake increased with increasing dietary soybean meal level. Crude protein and moisture content of liver tended to decrease and crude lipid content tended to increase with decreasing of dietary soybean meal levels. Plasma total cholesterol levels of fish fed the diets containing $20{\%}\;and\;30{\%}$ soybean meal were significantly lower than that of fish fed control diet (P < 0.05), Plasma GOT level significantly increased with increasing dietary soybean meal level (P < 0.05). It is concluded that soybean meal can be used as a partial substitute for fish meal up to $10{\%}$ in this dietary formulation for growth of juvenile flounder.

• Food Science and Biotechnology
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• v.17 no.3
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• pp.564-568
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• 2008

The effects of sodium copper chlorophyllin (SCC) on bioaccessibility and uptake of mercury from fish were investigated using an in vitro digestion coupled with a Caco-2 cell. Fish along with SCC was subjected to a simulated in vitro digestion, which simulates both the gastric and small intestinal phase in vivo. Mercury bioaccessibility, the amount of mercury released from fish to aqueous phase following a digestion, was measured. Various amounts of SCC (0.1-25 mg) significantly reduced mercury bioaccessibility in a dose dependent manner by 49-89% compared to the negative control (fish without SCC) (p<0.05). Mercury bioaccessibility in varying molar ratios of mercury to positive control, 2,3-dimercapto-1-propane sulfonate (DMPS) was between 24 and 52%. Mercury uptake by Caco-2 cells from test media containing aqueous phase following in vitro digestion was measured after 6 hr incubation at $37^{\circ}C$. Cellular mercury uptake with increasing amount of SCC ranged from 0.352 to $0.052\;{\mu}g$ mercury/mg protein, while those in DMPS treatment were between 0.14 and $0.27\;{\mu}g$ mercury/mg protein. Our study suggests that SCC can reduce mercury absorption following fish consumption and may be efficient as a synthetic chelating agent for long term chronic mercury exposure in fish eating populations.

• Journal of fish pathology
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• v.23 no.3
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• pp.273-280
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• 2010

To determine whether rock bream Oplegnathus fasciatus can be protected from rock bream iridovirus (RBIV) infection by intramuscular injection of long double-stranded RNAs (dsRNAs), we compared protective effect of virus-specific dsRNAs corresponding to major capsid protein (MCP), ORF 084, ORF 086 genes, and virus non-specific green fluorescent protein (GFP) gene. Furthermore, to determine whether the non-specific type I interferon (IFN) response was associated with protective effect, we estimated the activation of type I IFN response in fish using expression level of IFN inducible Mx gene as a marker. As a result, mortality of fish injected with dsRNAs and challenged with RBIV was delayed for a few days when comparing with PBS injected control group. However, virus-specific dsRNA injected groups exhibited no significant differences in survival period when compared to the GFP dsRNA injected group. Semi-quantitative analysis indicated that the degree of antiviral response via type I IFN response is supposedly equal among dsRNA injected fish. These results suggest that type I IFN response rather than sequence-specific RNA interference might involve in the lengthened survival period of fish injected with virus-specific dsRNAs.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.1
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• pp.123-128
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• 1992

In Experiment 1, when fasted chicks were fed diets containing various sources of protein for 3 days, the activities of lipogenic enzymes (acetyl-CoA carboxylase, fatty acid synthetase, citrate cleavage enzyme and malic enzyme) in the liver of growing chicks were significantly lower in the soybean protein or gluten diet than in the casein or fish protein diet. Triglycride contents of the liver and plasma of chicks fed the casein or fish protein diet were significantly lower than that of those fed soybean protein or gluten diet. In Experiment 2, the effects of dietary amino acid mixture simulating casein or protein on the activities of hepatic lipogenic enzymes were examined. The activities of acetyl-CoA carboxylase and fatty acid synthetase in the liver of chicks fed the casein diet were significantly higher than that of those fed the soybean protein diet or two diets of amino acid mixtures. Furthermore, there were no significant differences between the two diets of amino acid mixture based on casein or soybean protein. However, the activities of malic enzyme and citrate cleavage enzyme tended to be lower in the soybean-type amino acid diet than in the casein-type amino acid diet. Thus, some effects can be ascribed to the protein itself and some to the amino acid composition of the protein sources.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.8
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• pp.1045-1050
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• 2006

Optimum operation condition for pilot scale of alkaline processing for fish muscle was investigated by measuring protein solubility, yield, texture, and water-holding capacity. Recovered protein yield was 33.2% for whole fish and 61.8% for minced muscle. Optimum homogenized speed and time, using industrial scale homogenizer, were 3,000 rpm and 5 min, respectively. Limited centrifugal force of continuous cylinder type was 4,000 rpm for recovering soluble protein, and 2,000 rpm for recovering precipitated proteins. The pH control agents such as citric acid, sodium phosphate and calcium oxide decreased the breaking force and deformation of recovered protein gel. The breaking force and deformation of the recovered proteins were high compared to conventional surimi. The breaking force and deformation were decreased by addition of salt, starch and bovine plasma proteins. Whiteness of recovered protein gel was lower than that of conventional surimi. Alkaline processing greatly decreased nitrogen content and chemical oxygen demand in waste water. The results suggest that alkaline processing has a potential as industrial production for recovering the proteins from fish muscle.

• Journal of Aquaculture
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• v.18 no.3
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• pp.135-141
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• 2005

A 16-week feeding trial was conducted to estimate the optimum dietary protein to energy ratio (P/E ratio, mg/kcal) in juvenile Japanese eel, Anguilla japonica. Six experimental diets were formulated with three energy levels and two protein levels at each energy level. Three energy levels of 3800, 4150 and 4500 kcal per kg diets were included at 45 and 50% crude protein (CP) levels, respectively $(_{120}P_{45},\;_{110}P_{45},\;_{100}P_{45},\;_{130}P_{50},\;_{120}P_{50},\;and\;_{110}P_{50})$. After four weeks of the conditioning period, fish initially averaging $15.0{\pm}3g\;(means{\pm}SD)$ were randomly distributed into each tank as groups of 20 fish. Each diet was fed to fish in three randomly selected tanks at a rate of $2{\sim}3%$ wet body weight per day in the recirculated system. Weight gain (WG) and specific growth rate of fish fed diet $_{100}P_{45}$ were significantly higher (P<0.05) than those of fish fed the other diets. WG of fish fed diet $_{120}P_{50}$ was also significantly higher than those of fish fed diets $_{130}P_{50}$ and $_{110}P_{50}$. Feed efficiency ratio of fish fed diets $_{100}P_{45}$ and $_{110}P_{45}$ were significantly higher (P<0.05) than those of fish fed other diets. These results suggest that the optimum P/E ratio may be 100 mg/kcal with 45% protein diets, and 120 mg/Kcal 50% protein diets for the maximum growth of juvenile Japanese eel under the experimental condition.

• Journal of fish pathology
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• v.14 no.1
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• pp.54-58
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• 2001

The seasonal trends of plasma protein and glucose concentrations in marbled soles(Limanda yokohamae) and greenlings(Hexagrammos otakii), and the influence of transport stressor on those levels were investigated. Total plasma protein levels of marbled soles and greenlings in late spring and summer were significantly higher than those in winter(January). Plasma glucose levels were consistently increased according to elevation of water temperature both in marbled soles and greenlings. Transport stressor gave rise to decrease of plasma protein levels and increase of blood glucose levels.

• Journal of fish pathology
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• v.16 no.3
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• pp.161-164
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• 2003

An Infectious hematopoietic necrosis virus strain (IHNV-RTK) was isolated from cultured rainbow trout at Kumi and Jechun area in Korea during 2000 and 2001. In the RT-PCR amplification with the specific primer set designed from IHNV G protein region, a 540 bp PCR product was amplified from the RTK strain. The RTK strain showed higher sequence homology with the published IHNV G protein genes (RB-76, LR-73, Col-85, and Carson-89)

• Ocean and Polar Research
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• v.26 no.3
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• pp.415-423
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• 2004

An experiment was conducted to investigate the differences of total ammonia nitrogen (TAN) excretion rates and feces production rates among the offsprings from cultured Japanese strain (JJ, mean BW; $17.1{\pm}0.1g$), intraspecific hybrid strain between cultured Japanese and selected Korean strain (JK, mean BW: $17.1{\pm}0.1g$) and selected Korean strain(KK, mean BW: $21.5{\pm}0.1g$) of red sea bream in order to compare their dietary protein utilization efficiency. Fish were hand-fed with a commercial diet containing 46.7% crude protein for 2 weeks, three times daily 09:00, 13:00 and 17:00. After daily feeding, the TAN excretion rates reached peaks of 49.03, 58.75 and 36.26mg/kg fish/hr for the JJ, JK and KK strain, respectively, during the daytime. The value of the KK strain was significantly lower than that of the JJ and JX shuin (P<0.05), however daily TAN excretion rates of the JJ, JK and KK strain were not different (P>0.05). When fish were fed at satiation after 4 days of starvation, TAN excretion rates reached the maximum values 4 hours after the feeding fur the KK (31.23 mg/kg fish/hr) and 6 hours after the feeding fur the JJ (44.19 mg/tg fish/hr) and JK strain (41.70 mg/kg fish/hr). After 3 days of starvation, the daily endogenous TAU excretion rates (ETE) for the JJ, JK. and KK strain were 286.91, 215.66 and 179.29mg/kg fish/day, respectively. The value of the KK strain was lower than that of the JJ and JK strain (P<0.05). The total feces production rates of the JJ, JK and KK strain were not significantly different, however the proportions of feces production rates by time for the JJ, JK and KK strain were different (P<0.05). As overall results, efficiency of dietary protein utilization of JJ, JK and KK seems to be different and KK strain could offer a desirable option for aquaculture purpose.